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UniProtKB - P47011 (GLG2_YEAST)
Protein
Glycogenin-2
Gene
GLG2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Self-glucosylating initiator of glycogen synthesis. Catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues. These chains act as primers for the elongation reaction catalyzed by glycogen synthase. Capable of transferring glucosyl residues to unbound acceptors such as free oligoglucans or oligoglucan derivatives.
3 PublicationsMiscellaneous
Present with 981 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- L-tyrosyl-[glycogenin] + UDP-α-D-glucose = α-D-glucosyl-L-tyrosyl-[glycogenin] + H+ + UDP1 PublicationEC:2.4.1.1861 Publication
- [1,4-α-D-glucosyl](n)-L-tyrosyl-[glycogenin] + UDP-α-D-glucose = [1,4-α-D-glucosyl](n+1)-L-tyrosyl-[glycogenin] + H+ + UDP1 PublicationEC:2.4.1.1861 Publication
Cofactori
Mn2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 95 | SubstrateBy similarity | 1 | |
Sitei | 104 | Important for catalytic activityBy similarity | 1 | |
Metal bindingi | 120 | ManganeseBy similarity | 1 | |
Metal bindingi | 122 | ManganeseBy similarity | 1 | |
Metal bindingi | 249 | ManganeseBy similarity | 1 |
GO - Molecular functioni
- glycogenin glucosyltransferase activity Source: SGD
- glycosyltransferase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- UDP-alpha-D-glucose:glucosyl-glycogenin alpha-D-glucosyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- glycogen biosynthetic process Source: SGD
Keywordsi
Molecular function | Transferase |
Biological process | Glycogen biosynthesis |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-SCE-3322077, Glycogen synthesis R-SCE-6798695, Neutrophil degranulation R-SCE-70221, Glycogen breakdown (glycogenolysis) |
Protein family/group databases
CAZyi | GT8, Glycosyltransferase Family 8 |
Names & Taxonomyi
Protein namesi | Recommended name: Glycogenin-21 Publication (EC:2.4.1.1861 Publication)Alternative name(s): Glycogen synthesis initiator protein 21 Publication Glycogenin glucosyltransferase 21 Publication |
Gene namesi | Name:GLG21 Publication Ordered Locus Names:YJL137C ORF Names:J0663 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003673, GLG2 |
VEuPathDBi | FungiDB:YJL137C |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 230 | Y → F: Eliminates glycogen accumulation; when associated with F-232 and F-367. 1 Publication | 1 | |
Mutagenesisi | 232 | Y → F: Eliminates glycogen accumulation; when associated with F-230 and F-367. 1 Publication | 1 | |
Mutagenesisi | 367 | Y → F: Eliminates glycogen accumulation; when associated with F-230 and F-232. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215182 | 1 – 380 | Glycogenin-2Add BLAST | 380 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 230 | O-linked (Glc...) tyrosine1 Publication | 1 | |
Glycosylationi | 232 | O-linked (Glc...) tyrosine1 Publication | 1 | |
Glycosylationi | 367 | O-linked (Glc...) tyrosineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
MaxQBi | P47011 |
PaxDbi | P47011 |
PRIDEi | P47011 |
Interactioni
Subunit structurei
Interacts with glycogen synthase GSY2.
1 PublicationProtein-protein interaction databases
BioGRIDi | 33620, 53 interactors |
DIPi | DIP-1345N |
IntActi | P47011, 2 interactors |
MINTi | P47011 |
STRINGi | 4932.YJL137C |
Miscellaneous databases
RNActi | P47011, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 10 – 16 | Substrate bindingBy similarity | 7 | |
Regioni | 120 – 122 | Substrate bindingBy similarity | 3 | |
Regioni | 158 – 160 | Substrate bindingBy similarity | 3 | |
Regioni | 185 – 189 | Substrate bindingBy similarity | 5 | |
Regioni | 249 – 255 | Substrate bindingBy similarity | 7 | |
Regioni | 331 – 355 | DisorderedSequence analysisAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 338 – 352 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1950, Eukaryota |
GeneTreei | ENSGT00940000175485 |
HOGENOMi | CLU_017171_4_1_1 |
InParanoidi | P47011 |
OMAi | AGWPDIF |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR002495, Glyco_trans_8 IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF01501, Glyco_transf_8, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
i Sequence
Sequence statusi: Complete.
P47011-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKKVAICTL LYSRDYLPGA LTLAYQLQKL LKHAVVEDEI TLCLLIEKKL
60 70 80 90 100
FGDEFKPQEI ALIRSLFKEI IIIEPLKDQE KSIEKNKANL ELLKRPELSH
110 120 130 140 150
TLLKARLWEL VQFDQVLFLD ADTLPLNKEF FEILRLYPEQ TRFQIAAVPD
160 170 180 190 200
IGWPDMFNTG VLLLIPDLDM ATSLQDFLIK TVSIDGADQG IFNQFFNPIC
210 220 230 240 250
NYSKEVLHKV SPLMEWIRLP FTYNVTMPNY GYQSSPAMNF FQQHIRLIHF
260 270 280 290 300
IGTFKPWSRN TTDYDDHYYQ LWRSTQRELY SECHLSNYFT HLQLGNIETE
310 320 330 340 350
TNFYHEPPCL QDLLNHGKRE NQKHVDLDIT SVDRNASQKS TAEKHDIEKP
360 370 380
TSKPQSAFKF DWESTDYLDR VQRAFPKPDT
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 241 | Missing AA sequence (PubMed:8900126).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25436 Genomic DNA Translation: AAA91644.1 X87371 Genomic DNA Translation: CAA60818.1 Z49412 Genomic DNA Translation: CAA89432.1 BK006943 Genomic DNA Translation: DAA08663.1 |
PIRi | S55176 |
RefSeqi | NP_012398.1, NM_001181570.1 |
Genome annotation databases
EnsemblFungii | YJL137C_mRNA; YJL137C; YJL137C |
GeneIDi | 853304 |
KEGGi | sce:YJL137C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25436 Genomic DNA Translation: AAA91644.1 X87371 Genomic DNA Translation: CAA60818.1 Z49412 Genomic DNA Translation: CAA89432.1 BK006943 Genomic DNA Translation: DAA08663.1 |
PIRi | S55176 |
RefSeqi | NP_012398.1, NM_001181570.1 |
3D structure databases
SMRi | P47011 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33620, 53 interactors |
DIPi | DIP-1345N |
IntActi | P47011, 2 interactors |
MINTi | P47011 |
STRINGi | 4932.YJL137C |
Protein family/group databases
CAZyi | GT8, Glycosyltransferase Family 8 |
Proteomic databases
MaxQBi | P47011 |
PaxDbi | P47011 |
PRIDEi | P47011 |
Genome annotation databases
EnsemblFungii | YJL137C_mRNA; YJL137C; YJL137C |
GeneIDi | 853304 |
KEGGi | sce:YJL137C |
Organism-specific databases
SGDi | S000003673, GLG2 |
VEuPathDBi | FungiDB:YJL137C |
Phylogenomic databases
eggNOGi | KOG1950, Eukaryota |
GeneTreei | ENSGT00940000175485 |
HOGENOMi | CLU_017171_4_1_1 |
InParanoidi | P47011 |
OMAi | AGWPDIF |
Enzyme and pathway databases
Reactomei | R-SCE-3322077, Glycogen synthesis R-SCE-6798695, Neutrophil degranulation R-SCE-70221, Glycogen breakdown (glycogenolysis) |
Miscellaneous databases
PROi | PR:P47011 |
RNActi | P47011, protein |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR002495, Glyco_trans_8 IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF01501, Glyco_transf_8, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GLG2_YEAST | |
Accessioni | P47011Primary (citable) accession number: P47011 Secondary accession number(s): D6VW47 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - SIMILARITY comments
Index of protein domains and families