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UniProtKB - P46995 (SET2_YEAST)
Protein
Histone-lysine N-methyltransferase, H3 lysine-36 specific
Gene
SET2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Histone methyltransferase that trimethylates histone H3 'Lys-36' forming H3K36me3. Involved in transcription elongation as well as in transcription repression. The methyltransferase activity requires the recruitment to the RNA polymerase II, which is CTK1 dependent.
7 PublicationsMiscellaneous
Present with 217 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- L-lysyl36-[histone H3] + 3 S-adenosyl-L-methionine = 3 H+ + N6,N6,N6-trimethyl-L-lysyl36-[histone H3] + 3 S-adenosyl-L-homocysteinePROSITE-ProRule annotation3 PublicationsEC:2.1.1.359PROSITE-ProRule annotation3 Publications
GO - Molecular functioni
GO - Biological processi
- ascospore formation Source: SGD
- DNA-templated transcription, elongation Source: SGD
- DNA-templated transcription, termination Source: SGD
- histone deacetylation Source: SGD
- histone methylation Source: SGD
- negative regulation of antisense RNA transcription Source: SGD
- negative regulation of histone H3-K14 acetylation Source: SGD
- negative regulation of histone H3-K9 acetylation Source: SGD
- negative regulation of reciprocal meiotic recombination Source: SGD
- positive regulation of histone acetylation Source: SGD
- regulation of DNA-dependent DNA replication initiation Source: SGD
- regulation of histone exchange Source: SGD
- regulation of transcription, DNA-templated Source: SGD
Keywordsi
Molecular function | Methyltransferase, Repressor, Transferase |
Biological process | Transcription, Transcription regulation |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.359, 984 |
Reactomei | R-SCE-3214841, PKMTs methylate histone lysines R-SCE-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks |
Names & Taxonomyi
Protein namesi | Recommended name: Histone-lysine N-methyltransferase, H3 lysine-36 specific (EC:2.1.1.3593 Publications)Alternative name(s): Lysine N-methyltransferase 3 SET domain-containing protein 2 |
Gene namesi | Name:SET2 Synonyms:EZL1, KMT3 Ordered Locus Names:YJL168C ORF Names:J0520 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003704, SET2 |
VEuPathDBi | FungiDB:YJL168C |
Subcellular locationi
Nucleus
Other locations
- Chromosome Curated
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 195 | R → G: Reduces dramatically histone methyltransferase activity toward nucleosomes. 1 Publication | 1 | |
Mutagenesisi | 201 | C → A: Reduces dramatically histone methyltransferase activity toward nucleosomes. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186087 | 1 – 733 | Histone-lysine N-methyltransferase, H3 lysine-36 specificAdd BLAST | 733 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | PhosphoserineCombined sources | 1 | |
Modified residuei | 522 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P46995 |
PaxDbi | P46995 |
PRIDEi | P46995 |
PTM databases
iPTMneti | P46995 |
Interactioni
Subunit structurei
Interacts with the RNA polymerase II hyperphosphorylated CTD.
Interacts with CYC8.
7 PublicationsBinary interactionsi
P46995
With | #Exp. | IntAct |
---|---|---|
HHT2 [P61830] | 2 | EBI-16985,EBI-8098 |
Protein-protein interaction databases
BioGRIDi | 33591, 655 interactors |
DIPi | DIP-2150N |
IntActi | P46995, 62 interactors |
MINTi | P46995 |
STRINGi | 4932.YJL168C |
Miscellaneous databases
RNActi | P46995, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P46995 |
SMRi | P46995 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P46995 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 63 – 118 | AWSPROSITE-ProRule annotationAdd BLAST | 56 | |
Domaini | 120 – 237 | SETPROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 244 – 260 | Post-SETPROSITE-ProRule annotationAdd BLAST | 17 | |
Domaini | 475 – 507 | WWAdd BLAST | 33 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 32 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 504 – 548 | DisorderedSequence analysisAdd BLAST | 45 | |
Regioni | 619 – 718 | Binding to RNA polymerase II CTDAdd BLAST | 100 | |
Regioni | 672 – 692 | DisorderedSequence analysisAdd BLAST | 21 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 548 – 630 | Sequence analysisAdd BLAST | 83 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 15 – 32 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 504 – 531 | Polar residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 534 – 548 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Domaini
The AWS and SET domains are necessary for transcription repression.2 Publications
Sequence similaritiesi
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4442, Eukaryota |
HOGENOMi | CLU_008492_1_1_1 |
InParanoidi | P46995 |
OMAi | YANKYMG |
Family and domain databases
CDDi | cd19172, SET_SETD2, 1 hit |
Gene3Di | 1.10.1740.100, 1 hit |
InterProi | View protein in InterPro IPR006560, AWS_dom IPR003616, Post-SET_dom IPR025788, Set2_fungi IPR001214, SET_dom IPR044437, SETD2/Set2_SET IPR013257, SRI IPR038190, SRI_sf IPR001202, WW_dom IPR036020, WW_dom_sf |
Pfami | View protein in Pfam PF17907, AWS, 1 hit PF00856, SET, 1 hit PF08236, SRI, 1 hit PF18507, WW_1, 1 hit |
SMARTi | View protein in SMART SM00570, AWS, 1 hit SM00508, PostSET, 1 hit SM00317, SET, 1 hit SM00456, WW, 1 hit |
SUPFAMi | SSF51045, SSF51045, 1 hit |
PROSITEi | View protein in PROSITE PS51215, AWS, 1 hit PS50868, POST_SET, 1 hit PS51568, SAM_MT43_SET2_1, 1 hit PS50280, SET, 1 hit |
i Sequence
Sequence statusi: Complete.
P46995-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKNQSVSAS EDEKEILNNN AEGHKPQRLF DQEPDLTEEA LTKFENLDDC
60 70 80 90 100
IYANKRIGTF KNNDFMECDC YEEFSDGVNH ACDEDSDCIN RLTLIECVND
110 120 130 140 150
LCSSCGNDCQ NQRFQKKQYA PIAIFKTKHK GYGVRAEQDI EANQFIYEYK
160 170 180 190 200
GEVIEEMEFR DRLIDYDQRH FKHFYFMMLQ NGEFIDATIK GSLARFCNHS
210 220 230 240 250
CSPNAYVNKW VVKDKLRMGI FAQRKILKGE EITFDYNVDR YGAQAQKCYC
260 270 280 290 300
EEPNCIGFLG GKTQTDAASL LPQNIADALG VTVSMEKKWL KLKKLSGEPI
310 320 330 340 350
IKNENENINI EFLQSLEVQP IDSPVDVTKI MSVLLQQDNK IIASKLLKRL
360 370 380 390 400
FTIDDDSLRH QAIKLHGYTC FSKMLKLFIT EQPQVDGKGN ETEEDDIKFI
410 420 430 440 450
KGILDFLLEL PKTTRNGIES SQIDNVVKTL PAKFPFLKPN CDELLEKWSK
460 470 480 490 500
FETYKRITKK DINVAASKMI DLRRVRLPPG WEIIHENGRP LYYNAEQKTK
510 520 530 540 550
LHYPPSGSSK VFSSRSNTQV NSPSSSGIPK TPGALDSKKH KLSDEEYERK
560 570 580 590 600
KQKRLEYERI ALERAKQEEL ESLKQKLKLE NERKSVLEDI IAEANKQKEL
610 620 630 640 650
QKEEAKKLVE AKEAKRLKRK TVSQSQRLEH NWNKFFASFV PNLIKKNPQS
660 670 680 690 700
KQFDHENIKQ CAKDIVKILT TKELKKDSSR APPDDLTKGK RHKVKEFINS
710 720 730
YMDKIILKKK QKKALALSSA STRMSSPPPS TSS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 594 | A → F in CAA89464 (PubMed:8641269).Curated | 1 | |
Sequence conflicti | 605 | A → S in CAA89464 (PubMed:8641269).Curated | 1 | |
Sequence conflicti | 716 | A → G in CAA89464 (PubMed:8641269).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49444 Genomic DNA Translation: CAA89464.1 BK006943 Genomic DNA Translation: DAA08635.2 |
PIRi | S56951 |
RefSeqi | NP_012367.2, NM_001181601.2 |
Genome annotation databases
EnsemblFungii | YJL168C_mRNA; YJL168C; YJL168C |
GeneIDi | 853271 |
KEGGi | sce:YJL168C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49444 Genomic DNA Translation: CAA89464.1 BK006943 Genomic DNA Translation: DAA08635.2 |
PIRi | S56951 |
RefSeqi | NP_012367.2, NM_001181601.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1E0N | NMR | - | A | 479-505 | [»] | |
2C5Z | NMR | - | A | 620-719 | [»] | |
BMRBi | P46995 | |||||
SMRi | P46995 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33591, 655 interactors |
DIPi | DIP-2150N |
IntActi | P46995, 62 interactors |
MINTi | P46995 |
STRINGi | 4932.YJL168C |
PTM databases
iPTMneti | P46995 |
Proteomic databases
MaxQBi | P46995 |
PaxDbi | P46995 |
PRIDEi | P46995 |
Genome annotation databases
EnsemblFungii | YJL168C_mRNA; YJL168C; YJL168C |
GeneIDi | 853271 |
KEGGi | sce:YJL168C |
Organism-specific databases
SGDi | S000003704, SET2 |
VEuPathDBi | FungiDB:YJL168C |
Phylogenomic databases
eggNOGi | KOG4442, Eukaryota |
HOGENOMi | CLU_008492_1_1_1 |
InParanoidi | P46995 |
OMAi | YANKYMG |
Enzyme and pathway databases
BRENDAi | 2.1.1.359, 984 |
Reactomei | R-SCE-3214841, PKMTs methylate histone lysines R-SCE-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks |
Miscellaneous databases
EvolutionaryTracei | P46995 |
PROi | PR:P46995 |
RNActi | P46995, protein |
Family and domain databases
CDDi | cd19172, SET_SETD2, 1 hit |
Gene3Di | 1.10.1740.100, 1 hit |
InterProi | View protein in InterPro IPR006560, AWS_dom IPR003616, Post-SET_dom IPR025788, Set2_fungi IPR001214, SET_dom IPR044437, SETD2/Set2_SET IPR013257, SRI IPR038190, SRI_sf IPR001202, WW_dom IPR036020, WW_dom_sf |
Pfami | View protein in Pfam PF17907, AWS, 1 hit PF00856, SET, 1 hit PF08236, SRI, 1 hit PF18507, WW_1, 1 hit |
SMARTi | View protein in SMART SM00570, AWS, 1 hit SM00508, PostSET, 1 hit SM00317, SET, 1 hit SM00456, WW, 1 hit |
SUPFAMi | SSF51045, SSF51045, 1 hit |
PROSITEi | View protein in PROSITE PS51215, AWS, 1 hit PS50868, POST_SET, 1 hit PS51568, SAM_MT43_SET2_1, 1 hit PS50280, SET, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SET2_YEAST | |
Accessioni | P46995Primary (citable) accession number: P46995 Secondary accession number(s): D6VW19 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | September 21, 2011 | |
Last modified: | February 23, 2022 | |
This is version 200 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families