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Protein

Mitochondrial inner membrane protease subunit 2

Gene

IMP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic component of the mitochondrial inner membrane peptidase (IMP) complex. IMP catalyzes the removal of signal peptides required for the targeting of proteins from the mitochondrial matrix, across the inner membrane, into the inter-membrane space. The two catalytic IMP subunits seem to have non-overlapping substrate specificities. IMP2 substrates include nuclear encoded CYB2, mitochondrially encoded COX2 and cytochrome c1. Required for the stability of IMP1.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei41By similarity1
Active sitei91By similarity1

GO - Molecular functioni

  • endopeptidase activity Source: SGD
  • serine-type peptidase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease

Enzyme and pathway databases

BioCyciYEAST:G3O-32740-MONOMER

Protein family/group databases

MEROPSiS26.012

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial inner membrane protease subunit 2 (EC:3.4.21.-)
Gene namesi
Name:IMP2
Ordered Locus Names:YMR035W
ORF Names:YM9973.09
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR035W
SGDiS000004638 IMP2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei134 – 152HelicalSequence analysisAdd BLAST19

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi41S → A or T: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi91K → H or R: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi95G → D: Reduces processing of CYB2 and presence of IMP1 and SOM1 in the IMP complex. 1 Publication1
Mutagenesisi123G → S: Reduces processing of cytochrome c1; no effect on presence of IMP1 and SOM1 in the IMP complex. 1 Publication1
Mutagenesisi124D → N or Y: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi131D → N or E: Abolishes enzymatic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001095381 – 177Mitochondrial inner membrane protease subunit 2Add BLAST177

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

MaxQBiP46972
PaxDbiP46972
PRIDEiP46972

Interactioni

Subunit structurei

Component of the mitochondrial inner membrane peptidase (IMP) complex which at least consists of IMP1, IMP2 and SOM1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MDM30Q059302EBI-9231,EBI-31799

Protein-protein interaction databases

BioGridi35207, 304 interactors
DIPiDIP-1941N
IntActiP46972, 6 interactors
MINTiP46972
STRINGi4932.YMR035W

Structurei

3D structure databases

ProteinModelPortaliP46972
SMRiP46972
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S26 family. IMP2 subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075044
HOGENOMiHOG000003673
InParanoidiP46972
KOiK09648
OMAiYVFLSRW
OrthoDBiEOG092C52AJ

Family and domain databases

InterProiView protein in InterPro
IPR037730 IMP2
IPR036286 LexA/Signal_pep-like_sf
IPR000223 Pept_S26A_signal_pept_1
IPR019758 Pept_S26A_signal_pept_1_CS
IPR019756 Pept_S26A_signal_pept_1_Ser-AS
IPR015927 Peptidase_S24_S26A/B/C
PANTHERiPTHR12383:SF3 PTHR12383:SF3, 1 hit
PfamiView protein in Pfam
PF00717 Peptidase_S24, 1 hit
PRINTSiPR00727 LEADERPTASE
SUPFAMiSSF51306 SSF51306, 1 hit
PROSITEiView protein in PROSITE
PS00501 SPASE_I_1, 1 hit
PS00761 SPASE_I_3, 1 hit

Sequencei

Sequence statusi: Complete.

P46972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRAGSSKRF LRNTLIAISW VPVLLTINNN VVHIAQVKGT SMQPTLNPQT
60 70 80 90 100
ETLATDWVLL WKFGVKNPSN LSRDDIILFK APTNPRKVYC KRVKGLPFDT
110 120 130 140 150
IDTKFPYPKP QVNLPRGHIW VEGDNYFHSI DSNTFGPISS GLVIGKAITI
160 170
VWPPSRWGTD LKLSTGRDCI SKRAILE
Length:177
Mass (Da):19,930
Last modified:November 1, 1995 - v1
Checksum:i2335F334D8E9A552
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49213 Genomic DNA Translation: CAA89151.1
AY692994 Genomic DNA Translation: AAT93013.1
BK006946 Genomic DNA Translation: DAA09934.1
PIRiS53952
RefSeqiNP_013749.1, NM_001182532.1

Genome annotation databases

EnsemblFungiiYMR035W; YMR035W; YMR035W
GeneIDi855051
KEGGisce:YMR035W

Similar proteinsi

Entry informationi

Entry nameiIMP2_YEAST
AccessioniPrimary (citable) accession number: P46972
Secondary accession number(s): D6VZL0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 18, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

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