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Protein

DNA polymerase delta small subunit

Gene

POL31

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA polymerase delta (DNA polymerase III) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.1 Publication

Miscellaneous

Present with 626 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  • DNA binding Source: InterPro
  • DNA-directed DNA polymerase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionDNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-31652-MONOMER
BRENDAi2.7.7.7 984
ReactomeiR-SCE-69166 Removal of the Flap Intermediate
R-SCE-69183 Processive synthesis on the lagging strand

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase delta small subunit (EC:2.7.7.7)
Alternative name(s):
Hydroxyurea-sensitive protein 2
Gene namesi
Name:POL31
Synonyms:HUS2, HYS2, SDP5
Ordered Locus Names:YJR006W
ORF Names:J1427, YJR83.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

SGDiS000003766 POL31

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000961741 – 487DNA polymerase delta small subunitAdd BLAST487

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei20PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP46957
PaxDbiP46957
PRIDEiP46957

PTM databases

iPTMnetiP46957

Interactioni

Subunit structurei

DNA polymerase delta is a heterotrimer of POL3, POL32 and HYS2.

Binary interactionsi

WithEntry#Exp.IntActNotes
POL3P154365EBI-6080,EBI-6134

Protein-protein interaction databases

BioGridi33762, 474 interactors
ComplexPortaliCPX-2101 DNA polymerase delta complex
DIPiDIP-2525N
IntActiP46957, 5 interactors
MINTiP46957
STRINGi4932.YJR006W

Structurei

3D structure databases

ProteinModelPortaliP46957
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000006780
HOGENOMiHOG000189057
InParanoidiP46957
KOiK02328
OMAiDPANFML
OrthoDBiEOG092C3F88

Family and domain databases

InterProiView protein in InterPro
IPR007185 DNA_pol_alpha/epsilon_bsu
IPR024826 DNA_pol_delta/II_ssu
PANTHERiPTHR10416 PTHR10416, 1 hit
PfamiView protein in Pfam
PF04042 DNA_pol_E_B, 1 hit

Sequencei

Sequence statusi: Complete.

P46957-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDALLTKFNE DRSLQDENLS QPRTRVRIVD DNLYNKSNPF QLCYKKRDYG
60 70 80 90 100
SQYYHIYQYR LKTFRERVLK ECDKRWDAGF TLNGQLVLKK DKVLDIQGNQ
110 120 130 140 150
PCWCVGSIYC EMKYKPNVLD EVINDTYGAP DLTKSYTDKE GGSDEIMLED
160 170 180 190 200
ESGRVLLVGD FIRSTPFITG VVVGILGMEA EAGTFQVLDI CYPTPLPQNP
210 220 230 240 250
FPAPIATCPT RGKIALVSGL NLNNTSPDRL LRLEILREFL MGRINNKIDD
260 270 280 290 300
ISLIGRLLIC GNSVDFDIKS VNKDELMISL TEFSKFLHNI LPSISVDIMP
310 320 330 340 350
GTNDPSDKSL PQQPFHKSLF DKSLESYFNG SNKEILNLVT NPYEFSYNGV
360 370 380 390 400
DVLAVSGKNI NDICKYVIPS NDNGESENKV EEGESNDFKD DIEHRLDLME
410 420 430 440 450
CTMKWQNIAP TAPDTLWCYP YTDKDPFVLD KWPHVYIVAN QPYFGTRVVE
460 470 480
IGGKNIKIIS VPEFSSTGMI ILLDLETLEA ETVKIDI
Length:487
Mass (Da):55,296
Last modified:November 1, 1995 - v1
Checksum:iF9E200BFE97A2C07
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156L → H in BAA08859 (PubMed:7567461).Curated1
Sequence conflicti465S → N in BAA08859 (PubMed:7567461).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50324 Genomic DNA Translation: BAA08859.1
X87611 Genomic DNA Translation: CAA60928.1
Z49506 Genomic DNA Translation: CAA89528.1
BK006943 Genomic DNA Translation: DAA08797.1
PIRiS55194
RefSeqiNP_012539.1, NM_001181663.1

Genome annotation databases

EnsemblFungiiYJR006W; YJR006W; YJR006W
GeneIDi853462
KEGGisce:YJR006W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50324 Genomic DNA Translation: BAA08859.1
X87611 Genomic DNA Translation: CAA60928.1
Z49506 Genomic DNA Translation: CAA89528.1
BK006943 Genomic DNA Translation: DAA08797.1
PIRiS55194
RefSeqiNP_012539.1, NM_001181663.1

3D structure databases

ProteinModelPortaliP46957
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33762, 474 interactors
ComplexPortaliCPX-2101 DNA polymerase delta complex
DIPiDIP-2525N
IntActiP46957, 5 interactors
MINTiP46957
STRINGi4932.YJR006W

PTM databases

iPTMnetiP46957

Proteomic databases

MaxQBiP46957
PaxDbiP46957
PRIDEiP46957

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR006W; YJR006W; YJR006W
GeneIDi853462
KEGGisce:YJR006W

Organism-specific databases

SGDiS000003766 POL31

Phylogenomic databases

GeneTreeiENSGT00390000006780
HOGENOMiHOG000189057
InParanoidiP46957
KOiK02328
OMAiDPANFML
OrthoDBiEOG092C3F88

Enzyme and pathway databases

BioCyciYEAST:G3O-31652-MONOMER
BRENDAi2.7.7.7 984
ReactomeiR-SCE-69166 Removal of the Flap Intermediate
R-SCE-69183 Processive synthesis on the lagging strand

Miscellaneous databases

PROiPR:P46957

Family and domain databases

InterProiView protein in InterPro
IPR007185 DNA_pol_alpha/epsilon_bsu
IPR024826 DNA_pol_delta/II_ssu
PANTHERiPTHR10416 PTHR10416, 1 hit
PfamiView protein in Pfam
PF04042 DNA_pol_E_B, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPOD2_YEAST
AccessioniPrimary (citable) accession number: P46957
Secondary accession number(s): D6VWI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 7, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names
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Main funding by: National Institutes of Health

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