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UniProtKB - P46935 (NEDD4_MOUSE)

Entry version 205 (29 Sep 2021)
Sequence version 3 (20 Jun 2003)
Previous versions | rss
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Protein

E3 ubiquitin-protein ligase NEDD4

Gene

Nedd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (By similarity).

Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Involved in ubiquitination of ERBB4 intracellular domain E4ICD1 (PubMed:19193720).

Predominantly involved in ubiquitination of membrane bound forms of ERBB4 rather than processed precursors and intermediate membrane-anchored 80 kDa fragments (m80HER4), with a lesser role in ubiquitination of ERBB4 intracellular domain E4ICD1 (PubMed:19047365).

Promotes ubiquitination of RAPGEF2. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2 (By similarity).

Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (By similarity).

By similarity2 Publications

Miscellaneous

A cysteine residue is required for ubiquitin-thioester formation.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity EC:2.3.2.26

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Activated by NDFIP1- and NDFIP2-binding.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei854Glycyl thioester intermediate1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTransferase
Biological processNeurogenesis, Ubl conjugation pathway

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-MMU-1169408, ISG15 antiviral mechanism
R-MMU-1253288, Downregulation of ERBB4 signaling
R-MMU-8948747, Regulation of PTEN localization
R-MMU-8948751, Regulation of PTEN stability and activity
R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00143

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
E3 ubiquitin-protein ligase NEDD4 (EC:2.3.2.26By similarity)
Alternative name(s):
HECT-type E3 ubiquitin transferase NEDD4
Neural precursor cell expressed developmentally down-regulated protein 4
Short name:
NEDD-4
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Nedd4
Synonyms:Kiaa0093, Nedd-4, Nedd4-1, Nedd4a
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 9

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...MGIi		MGI:97297, Nedd4

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSMUSG00000032216

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Lethal during late gestation. Embryos show a retarded development and defects in vasculogenesis and angiogenesis.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi854C → S: Loss of ubiquitin-ligase activity. No effect on VEGFR-2/KDFR degradation. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001203201 – 887E3 ubiquitin-protein ligase NEDD4Add BLAST887

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei215PhosphoserineBy similarity1
Modified residuei287PhosphothreonineCombined sources1
Modified residuei309PhosphoserineCombined sources1
Modified residuei380PhosphoserineBy similarity1
Modified residuei385PhosphoserineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Auto-ubiquitinated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		P46935

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P46935

MaxQB - The MaxQuant DataBase

More...MaxQBi		P46935

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P46935

PRoteomics IDEntifications database

More...PRIDEi		P46935

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		252877

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P46935

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P46935

SwissPalm database of S-palmitoylation events

More...SwissPalmi		P46935

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Brain.

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSMUSG00000032216, Expressed in metanephric loop of Henle and 334 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		P46935, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		P46935, MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Binds SCNN1A, SCNN1B and SCNN1G.

Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase and may induce its recruitment to exosomes.

Interacts with UBE2D2 (PubMed:9182527). Binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif (PubMed:9407065).

Interacts with BEAN1, LITAF, RNF11, WBP1, WBP2, PMEPAI and PRRG2 (PubMed:11042109).

Interacts with murine leukemia virus Gag polyprotein (via PPXY motif) (PubMed:15908698).

Interacts (via C2 domain) with GRB10 (via SH2 domain) (PubMed:12697834, PubMed:15060076, PubMed:18286479, PubMed:20980250).

Interacts with ERBB4 (PubMed:19193720, PubMed:19047365).

Interacts with TNIK; the interaction is direct, allows the TNIK-dependent recruitment of RAP2A and its ubiquitination by NEDD4 (PubMed:20159449).

Interacts (via WW3 domain) with TNK2; EGF promotes this interaction.

Interacts (via WW3 domain) with FGFR1 (via C-terminus).

Interacts with OTUD7B (By similarity).

Interacts with ISG15 (By similarity).

Interacts (via WW domain) with RAPGEF2; this interaction leads to ubiquitination and degradation via the proteasome pathway.

Interacts (via WW domains) with ARRDC3 (via PPXY motifs) (By similarity).

Interacts with LAPTM4B; may play a role in the lysosomal sorting of LAPTM4B (By similarity).

Interacts with ZBTB7B (PubMed:28784777).

Interacts with PRRG4 (via cytoplasmic domain) (By similarity).

By similarity12 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		201723, 82 interactors

Database of interacting proteins

More...DIPi		DIP-32323N

The Eukaryotic Linear Motif resource for Functional Sites in Proteins

More...ELMi		P46935

Protein interaction database and analysis system

More...IntActi		P46935, 22 interactors

Molecular INTeraction database

More...MINTi		P46935

STRING: functional protein association networks

More...STRINGi		10090.ENSMUSP00000034740

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P46935, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1887
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P46935

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini58 – 183C2PROSITE-ProRule annotationAdd BLAST126
Domaini249 – 282WW 1PROSITE-ProRule annotationAdd BLAST34
Domaini405 – 438WW 2PROSITE-ProRule annotationAdd BLAST34
Domaini460 – 493WW 3PROSITE-ProRule annotationAdd BLAST34
Domaini552 – 887HECTPROSITE-ProRule annotationAdd BLAST336

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni217 – 549Mediates interaction with TNIK1 PublicationAdd BLAST333
Regioni236 – 257DisorderedSequence analysisAdd BLAST22
Regioni271 – 290DisorderedSequence analysisAdd BLAST20
Regioni302 – 322DisorderedSequence analysisAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi271 – 285Basic and acidic residuesSequence analysisAdd BLAST15
Compositional biasi306 – 322Basic and acidic residuesSequence analysisAdd BLAST17

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The WW domains mediate interaction with PPxY motif-containing proteins (By similarity). The WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, PMEPAI, NDFIP1 and PRRG2 (PubMed:11042109).By similarity1 Publication

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG0940, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000158905

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_002173_0_3_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P46935

Identification of Orthologs from Complete Genome Data

More...OMAi		PRFENTN

Database of Orthologous Groups

More...OrthoDBi		271539at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		P46935

TreeFam database of animal gene trees

More...TreeFami		TF323658

Family and domain databases

Conserved Domains Database

More...CDDi		cd00078, HECTc, 1 hit
cd00201, WW, 3 hits

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		2.60.40.150, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR000008, C2_dom
IPR035892, C2_domain_sf
IPR024928, E3_ub_ligase_SMURF1
IPR000569, HECT_dom
IPR035983, Hect_E3_ubiquitin_ligase
IPR001202, WW_dom
IPR036020, WW_dom_sf

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00168, C2, 1 hit
PF00632, HECT, 1 hit
PF00397, WW, 3 hits

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF001569, E3_ub_ligase_SMURF1, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00239, C2, 1 hit
SM00119, HECTc, 1 hit
SM00456, WW, 3 hits

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF49562, SSF49562, 1 hit
SSF51045, SSF51045, 3 hits
SSF56204, SSF56204, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50004, C2, 1 hit
PS50237, HECT, 1 hit
PS01159, WW_DOMAIN_1, 3 hits
PS50020, WW_DOMAIN_2, 3 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

P46935-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSDMAADES EAPVLSEDEV WEFCLDKTED GGGSPGSDVT DTCEPPCGCW 
        60         70         80         90        100
ELNPNSLEEE HVLFTADPYL ELHNDDTRVV RVKVIAGIGL AKKDILGASD 
       110        120        130        140        150
PYVRVTLYDP MSGILTSVQT KTIKKSLNPK WNEEILFRVL PQRHRILFEV 
       160        170        180        190        200
FDENRLTRDD FLGQVDVPLY PLPTENPRME RPYTFKDFVL HPRSHKSRVK 
       210        220        230        240        250
GYLRLKMTYL PKNGSEDENA DQAEELEPGW VVLDQPDAAT HLPHPPEPSP 
       260        270        280        290        300
LPPGWEERQD VLGRTYYVNH ESRRTQWKRP SPDDDLTDED NDDMQLQAQR 
       310        320        330        340        350
AFTTRRQISE DVDGPDNRES PENWEIVRED ENTEYSGQAV QSPPSGHIDV 
       360        370        380        390        400
QTHLAEEFNT RLAVCGNPAT SQPVTSSNHS SRGGSLQTCI FEEQPTLPVL 
       410        420        430        440        450
LPTSSGLPPG WEEKQDDRGR SYYVDHNSKT TTWSKPTMQD DPRSKIPAHL 
       460        470        480        490        500
RGKTDSNDLG PLPPGWEERT HTDGRVFFIN HNIKKTQWED PRLQNVAITG 
       510        520        530        540        550
PAVPYSRDYK RKYEFFRRKL KKQTDIPNKF EMKLRRANIL EDSYRRIMGV 
       560        570        580        590        600
KRADLLKARL WIEFDGEKGL DYGGVAREWF FLISKEMFNP YYGLFEYSAT 
       610        620        630        640        650
DNYTLQINPN SGLCNEDHLS YFKFIGRVAG MAVYHGKLLD GFFIRPFYKM 
       660        670        680        690        700
MLQKLITLHD MESVDSEYYS SLRWILENDP TELDLRFIID EELFGQTHQH 
       710        720        730        740        750
ELKTGGSEIV VTNKNKKEYI YLVIQWRFVN RIQKQMAAFK EGFFELIPQD 
       760        770        780        790        800
LIKIFDENEL ELLMCGLGDV DVNDWREHTK YKNGYSMNHQ VIHWFWKAVW 
       810        820        830        840        850
MMDSEKRIRL LQFVTGTSRV PMNGFAELYG SNGPQSFTVE QWGTPDKLPR 
       860        870        880 
AHTCFNRLDL PPYESFDELW DKLQMAIENT QGFDGVD
Length:887
Mass (Da):102,706
Last modified:June 20, 2003 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iAE7DD3ED63986C50
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A571BDN7A0A571BDN7_MOUSE
HECT-type E3 ubiquitin transferase
Nedd4
1,136Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A571BEP7A0A571BEP7_MOUSE
E3 ubiquitin-protein ligase NEDD4
Nedd4
496Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
V9GXK3V9GXK3_MOUSE
E3 ubiquitin-protein ligase NEDD4
Nedd4
195Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
Q3V335Q3V335_MOUSE
E3 ubiquitin-protein ligase NEDD4
Nedd4
305Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
V9GWV8V9GWV8_MOUSE
E3 ubiquitin-protein ligase NEDD4
Nedd4
196Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
V9GXS6V9GXS6_MOUSE
E3 ubiquitin-protein ligase NEDD4
Nedd4
30Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAB63360 differs from that shown. Reason: Frameshift.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
D85414 mRNA Translation: BAA12803.1
U96635 mRNA Translation: AAB63360.1 Frameshift.
AK088620 mRNA Translation: BAC40458.1
AK088767 mRNA Translation: BAC40558.1
AK122203 mRNA Translation: BAC65485.1
AK133838 mRNA Translation: BAE21875.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS72275.1

NCBI Reference Sequences

More...RefSeqi		NP_035020.2, NM_010890.3

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENSMUST00000034740; ENSMUSP00000034740; ENSMUSG00000032216

Database of genes from NCBI RefSeq genomes

More...GeneIDi		17999

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		mmu:17999

UCSC genome browser

More...UCSCi		uc009qqe.1, mouse

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85414 mRNA Translation: BAA12803.1
U96635 mRNA Translation: AAB63360.1 Frameshift.
AK088620 mRNA Translation: BAC40458.1
AK088767 mRNA Translation: BAC40558.1
AK122203 mRNA Translation: BAC65485.1
AK133838 mRNA Translation: BAE21875.1
CCDSiCCDS72275.1
RefSeqiNP_035020.2, NM_010890.3

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M7FX-ray2.00B71-246[»]
SMRiP46935
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi201723, 82 interactors
DIPiDIP-32323N
ELMiP46935
IntActiP46935, 22 interactors
MINTiP46935
STRINGi10090.ENSMUSP00000034740

PTM databases

iPTMnetiP46935
PhosphoSitePlusiP46935
SwissPalmiP46935

Proteomic databases

EPDiP46935
jPOSTiP46935
MaxQBiP46935
PaxDbiP46935
PRIDEiP46935
ProteomicsDBi252877

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		25114, 284 antibodies

The DNASU plasmid repository

More...DNASUi		17999

Genome annotation databases

EnsembliENSMUST00000034740; ENSMUSP00000034740; ENSMUSG00000032216
GeneIDi17999
KEGGimmu:17999
UCSCiuc009qqe.1, mouse

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		4734
MGIiMGI:97297, Nedd4
VEuPathDBiHostDB:ENSMUSG00000032216

Rodent Unidentified Gene-Encoded large proteins database

More...Rougei		Search...

Phylogenomic databases

eggNOGiKOG0940, Eukaryota
GeneTreeiENSGT00940000158905
HOGENOMiCLU_002173_0_3_1
InParanoidiP46935
OMAiPRFENTN
OrthoDBi271539at2759
PhylomeDBiP46935
TreeFamiTF323658

Enzyme and pathway databases

UniPathwayiUPA00143
ReactomeiR-MMU-1169408, ISG15 antiviral mechanism
R-MMU-1253288, Downregulation of ERBB4 signaling
R-MMU-8948747, Regulation of PTEN localization
R-MMU-8948751, Regulation of PTEN stability and activity
R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		17999, 0 hits in 57 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		Nedd4, mouse

Protein Ontology

More...PROi		PR:P46935
RNActiP46935, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSMUSG00000032216, Expressed in metanephric loop of Henle and 334 other tissues
ExpressionAtlasiP46935, baseline and differential
GenevisibleiP46935, MM

Family and domain databases

CDDicd00078, HECTc, 1 hit
cd00201, WW, 3 hits
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008, C2_dom
IPR035892, C2_domain_sf
IPR024928, E3_ub_ligase_SMURF1
IPR000569, HECT_dom
IPR035983, Hect_E3_ubiquitin_ligase
IPR001202, WW_dom
IPR036020, WW_dom_sf
PfamiView protein in Pfam
PF00168, C2, 1 hit
PF00632, HECT, 1 hit
PF00397, WW, 3 hits
PIRSFiPIRSF001569, E3_ub_ligase_SMURF1, 1 hit
SMARTiView protein in SMART
SM00239, C2, 1 hit
SM00119, HECTc, 1 hit
SM00456, WW, 3 hits
SUPFAMiSSF49562, SSF49562, 1 hit
SSF51045, SSF51045, 3 hits
SSF56204, SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50004, C2, 1 hit
PS50237, HECT, 1 hit
PS01159, WW_DOMAIN_1, 3 hits
PS50020, WW_DOMAIN_2, 3 hits

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNEDD4_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P46935
Secondary accession number(s): O08758, Q3UZI2, Q8BGB3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 20, 2003
Last modified: September 29, 2021
This is version 205 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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