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UniProtKB - P46919 (GPDA_BACSU)
Protein
Glycerol-3-phosphate dehydrogenase [NAD(P)+]
Gene
gpsA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Involved in the biosynthesis of the sn-glycerol 3-phosphate required for phospholipid synthesis.
1 PublicationCatalytic activityi
Activity regulationi
Slightly inhibited by sn-glycerol 3-phosphate.1 Publication
: glycerophospholipid metabolism Pathwayi
This protein is involved in the pathway glycerophospholipid metabolism, which is part of Membrane lipid metabolism.UniRule annotationView all proteins of this organism that are known to be involved in the pathway glycerophospholipid metabolism and in Membrane lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 106 | NAD; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 106 | SubstrateUniRule annotation | 1 | |
Binding sitei | 141 | NAD; via amide nitrogenUniRule annotation | 1 | |
Active sitei | 192 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 256 | NADUniRule annotation | 1 | |
Binding sitei | 282 | NADUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 8 – 13 | NADUniRule annotation | 6 |
GO - Molecular functioni
- glycerol-3-phosphate dehydrogenase [NAD(P)+] activity Source: UniProtKB
- glycerol-3-phosphate dehydrogenase [NAD+] activity Source: InterPro
- glycerol-3-phosphate dehydrogenase [NADP+] activity Source: RHEA
- NAD binding Source: InterPro
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- glycerol-3-phosphate biosynthetic process Source: UniProtKB-UniRule
- glycerol-3-phosphate catabolic process Source: InterPro
- glycerol-3-phosphate metabolic process Source: GO_Central
- glycerophospholipid metabolic process Source: UniProtKB-UniPathway
- phospholipid biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | BSUB:BSU22830-MONOMER |
UniPathwayi | UPA00940 |
Names & Taxonomyi
Protein namesi | Recommended name: Glycerol-3-phosphate dehydrogenase [NAD(P)+]UniRule annotation (EC:1.1.1.94UniRule annotation)Alternative name(s): NAD(P)H-dependent dihydroxyacetone-phosphate reductase NAD(P)H-dependent glycerol-3-phosphate dehydrogenaseUniRule annotation |
Gene namesi | Name:gpsAUniRule annotation Synonyms:glyC Ordered Locus Names:BSU22830 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Cytosol
- cytosol Source: GO_Central
Other locations
- glycerol-3-phosphate dehydrogenase complex Source: InterPro
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Cells lacking this gene are auxotrophic for glycerol.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000137926 | 1 – 345 | Glycerol-3-phosphate dehydrogenase [NAD(P)+]Add BLAST | 345 |
Proteomic databases
jPOSTi | P46919 |
PaxDbi | P46919 |
PRIDEi | P46919 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 256 – 257 | Substrate bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0240, Bacteria |
InParanoidi | P46919 |
OMAi | NRMFGNM |
PhylomeDBi | P46919 |
Family and domain databases
Gene3Di | 1.10.1040.10, 1 hit |
HAMAPi | MF_00394, NAD_Glyc3P_dehydrog, 1 hit |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR013328, 6PGD_dom2 IPR006168, G3P_DH_NAD-dep IPR006109, G3P_DH_NAD-dep_C IPR011128, G3P_DH_NAD-dep_N IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF07479, NAD_Gly3P_dh_C, 1 hit PF01210, NAD_Gly3P_dh_N, 1 hit |
PIRSFi | PIRSF000114, Glycerol-3-P_dh, 1 hit |
PRINTSi | PR00077, GPDHDRGNASE |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00957, NAD_G3PDH, 1 hit |
i Sequence
Sequence statusi: Complete.
P46919-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKVTMLGAG SWGTALALVL TDNGNEVCVW AHRADLIHQI NELHENKDYL
60 70 80 90 100
PNVKLSTSIK GTTDMKEAVS DADVIIVAVP TKAIREVLRQ AVPFITKKAV
110 120 130 140 150
FVHVSKGIEP DSLLRISEIM EIELPSDVRK DIVVLSGPSH AEEVGLRHPT
160 170 180 190 200
TVTASSKSMR AAEEVQDLFI NHNFRVYTNP DIIGVEIGGA LKNIIALAAG
210 220 230 240 250
ITDGLGYGDN AKAALITRGL AEIARLGTKM GGNPLTFSGL TGVGDLIVTC
260 270 280 290 300
TSVHSRNWRA GNLLGKGYKL EDVLEEMGMV VEGVRTTKAA YQLSKKYDVK
310 320 330 340
MPITEALHQV LFNGQKVETA VESLMARGKT HEMEDLVNTF ENQVK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 130 | K → R in AAA86746 (PubMed:7592341).Curated | 1 | |
Sequence conflicti | 149 | P → A in AAA86746 (PubMed:7592341).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32164 Genomic DNA Translation: AAA86746.1 L47648 Genomic DNA Translation: AAC83967.1 AL009126 Genomic DNA Translation: CAB14199.1 |
PIRi | H69636 |
RefSeqi | NP_390164.1, NC_000964.3 WP_003230566.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14199; CAB14199; BSU_22830 |
GeneIDi | 938989 |
KEGGi | bsu:BSU22830 |
PATRICi | fig|224308.179.peg.2488 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U32164 Genomic DNA Translation: AAA86746.1 L47648 Genomic DNA Translation: AAC83967.1 AL009126 Genomic DNA Translation: CAB14199.1 |
PIRi | H69636 |
RefSeqi | NP_390164.1, NC_000964.3 WP_003230566.1, NZ_JNCM01000036.1 |
3D structure databases
AlphaFoldDBi | P46919 |
SMRi | P46919 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU22830 |
Proteomic databases
jPOSTi | P46919 |
PaxDbi | P46919 |
PRIDEi | P46919 |
Genome annotation databases
EnsemblBacteriai | CAB14199; CAB14199; BSU_22830 |
GeneIDi | 938989 |
KEGGi | bsu:BSU22830 |
PATRICi | fig|224308.179.peg.2488 |
Phylogenomic databases
eggNOGi | COG0240, Bacteria |
InParanoidi | P46919 |
OMAi | NRMFGNM |
PhylomeDBi | P46919 |
Enzyme and pathway databases
UniPathwayi | UPA00940 |
BioCyci | BSUB:BSU22830-MONOMER |
Family and domain databases
Gene3Di | 1.10.1040.10, 1 hit |
HAMAPi | MF_00394, NAD_Glyc3P_dehydrog, 1 hit |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR013328, 6PGD_dom2 IPR006168, G3P_DH_NAD-dep IPR006109, G3P_DH_NAD-dep_C IPR011128, G3P_DH_NAD-dep_N IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF07479, NAD_Gly3P_dh_C, 1 hit PF01210, NAD_Gly3P_dh_N, 1 hit |
PIRSFi | PIRSF000114, Glycerol-3-P_dh, 1 hit |
PRINTSi | PR00077, GPDHDRGNASE |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00957, NAD_G3PDH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GPDA_BACSU | |
Accessioni | P46919Primary (citable) accession number: P46919 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | August 29, 2003 | |
Last modified: | May 25, 2022 | |
This is version 156 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families