UniProtKB - P46863 (KL61_DROME)
Kinesin-like protein Klp61F
Klp61F
Functioni
Important role in mitotic dividing cells (PubMed:8227131).
Microtubule motor required for spindle body separation (PubMed:8918872).
Slow plus-end directed microtubule motor capable of cross-linking and sliding apart antiparallel microtubules, thereby pushing apart the associated spindle poles during spindle assembly and function (PubMed:8918872, PubMed:8589456, PubMed:19062285).
Forms cross-links between microtubules within interpolar microtubule bundles (PubMed:9885249, PubMed:19158379).
Contributes to the length of the metaphase spindle, maintains the prometaphase spindle by antagonizing Ncd, drives anaphase B, and also contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates (PubMed:19158379).
Displays microtubule-stimulated ATPase activity (PubMed:8589456).
Required for normal fusome organization (PubMed:10469596).
Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769).
8 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 103 – 110 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cytoskeletal motor activity Source: FlyBase
- microtubule binding Source: UniProtKB
- plus-end-directed microtubule motor activity Source: UniProtKB
GO - Biological processi
- cell division Source: UniProtKB-KW
- centrosome separation Source: FlyBase
- fusome organization Source: UniProtKB
- Golgi organization Source: FlyBase
- microtubule-based movement Source: UniProtKB
- microtubule bundle formation Source: FlyBase
- mitotic cell cycle Source: FlyBase
- mitotic centrosome separation Source: FlyBase
- mitotic spindle assembly Source: GO_Central
- mitotic spindle organization Source: FlyBase
- plus-end directed microtubule sliding Source: FlyBase
- positive regulation of Golgi to plasma membrane protein transport Source: UniProtKB
- protein secretion Source: FlyBase
Keywordsi
Molecular function | Motor protein |
Biological process | Cell cycle, Cell division, Mitosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-DME-983189, Kinesins |
SignaLinki | P46863 |
Names & Taxonomyi
Protein namesi | Recommended name: Kinesin-like protein Klp61F1 PublicationAlternative name(s): Bipolar kinesin KRP-1301 Publication |
Gene namesi | ORF Names:CG9191Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0004378, Klp61F |
VEuPathDBi | VectorBase:FBgn0004378 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Cytoskeleton
- spindle 4 Publications
- spindle pole 1 Publication
Note: In somatic cells, cytoplasmic during interphase, localized to centrosomal asters at the onset of mitosis in prophase and associated with spindle structures during the remainder of mitosis (PubMed:10469596). In male and female germ cells, associates with fusomes during interphase, then localizes to centrosomal asters during prophase and to spindles in metaphase (PubMed:10469596). Coincident with spindle microtubules from prophase to metaphase and, as mitosis proceeds, found both at the spindle poles and along the spindle fibers (PubMed:8589456).2 Publications
Cytoskeleton
- aster Source: UniProtKB
- kinesin complex Source: FlyBase
- microtubule Source: FlyBase
- microtubule associated complex Source: FlyBase
- mitotic spindle Source: UniProtKB
- mitotic spindle microtubule Source: FlyBase
- mitotic spindle pole Source: UniProtKB
- spindle Source: FlyBase
- spindle microtubule Source: GO_Central
Endoplasmic reticulum
- endoplasmic reticulum Source: FlyBase
Golgi apparatus
- Golgi apparatus Source: FlyBase
Nucleus
- nucleus Source: UniProtKB
Other locations
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, MicrotubulePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 23 | Y → F: Spindle defects and greatly reduced phosphorylation by Wee1 in vitro; when associated with F-152 and F-207. 1 Publication | 1 | |
Mutagenesisi | 152 | Y → F: Spindle defects and greatly reduced phosphorylation by Wee1 in vitro; when associated with F-23 and F-207. 1 Publication | 1 | |
Mutagenesisi | 207 | Y → F: Spindle defects and greatly reduced phosphorylation by Wee1 in vitro; when associated with F-23 and F-152. 1 Publication | 1 | |
Mutagenesisi | 669 | F → E: Remains tetrameric. 1 Publication | 1 | |
Mutagenesisi | 726 | L → D: Mainly tetrameric. Mainly dimeric; when associated with R-775. 1 Publication | 1 | |
Mutagenesisi | 726 | L → K: Mainly tetrameric. 1 Publication | 1 | |
Mutagenesisi | 729 – 730 | MM → EE: Mainly monomeric. 1 Publication | 2 | |
Mutagenesisi | 740 | R → A: Formation of tetramers, dimers and monomers. 1 Publication | 1 | |
Mutagenesisi | 761 | R → A: Formation of tetramers and dimers. 1 Publication | 1 | |
Mutagenesisi | 775 | Y → R: Mainly tetrameric with formation of some monomers. Mainly dimeric; when associated with D-726. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL1795160 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000125371 | 1 – 1066 | Kinesin-like protein Klp61FAdd BLAST | 1066 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 520 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 933 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 949 | Phosphoserine2 Publications | 1 | |
Modified residuei | 1043 | Phosphoserine1 Publication | 1 | |
Modified residuei | 1045 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 1050 | Phosphoserine1 Publication | 1 | |
Modified residuei | 1054 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P46863 |
PRIDEi | P46863 |
PTM databases
iPTMneti | P46863 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | FBgn0004378, Expressed in embryo and 41 other tissues |
ExpressionAtlasi | P46863, baseline and differential |
Genevisiblei | P46863, DM |
Interactioni
Subunit structurei
Homotetramer (PubMed:8538794, PubMed:9885249, PubMed:24714498). Consists of two pairs of polypeptides associated by coiled-coil interactions to form two homodimers (PubMed:8538794). The homodimers are linked by lateral interactions between their coiled-coil regions to form a bipolar homotetramer consisting of a central rod with two motor domains projecting from either end (PubMed:8538794). Parallel coiled coils extend from each pair of motor heads, switch to two antiparallel coiled coils in the central region and then back to parallel coiled coils (PubMed:24714498).
Interacts with Wee1 (PubMed:19800237).
4 PublicationsGO - Molecular functioni
- microtubule binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 63681, 15 interactors |
DIPi | DIP-22047N |
IntActi | P46863, 9 interactors |
MINTi | P46863 |
STRINGi | 7227.FBpp0072616 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P46863 |
SMRi | P46863 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P46863 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 19 – 356 | Kinesin motorPROSITE-ProRule annotationAdd BLAST | 338 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 990 – 1009 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 1016 – 1066 | DisorderedSequence analysisAdd BLAST | 51 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 362 – 462 | Sequence analysisAdd BLAST | 101 | |
Coiled coili | 540 – 569 | Sequence analysisAdd BLAST | 30 | |
Coiled coili | 639 – 738 | Sequence analysisAdd BLAST | 100 | |
Coiled coili | 808 – 875 | Sequence analysisAdd BLAST | 68 | |
Coiled coili | 889 – 918 | Sequence analysisAdd BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1016 – 1054 | Polar residuesSequence analysisAdd BLAST | 39 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0243, Eukaryota |
GeneTreei | ENSGT00940000155921 |
HOGENOMi | CLU_001485_33_4_1 |
InParanoidi | P46863 |
OMAi | PGHKDIE |
OrthoDBi | 179272at2759 |
PhylomeDBi | P46863 |
Family and domain databases
Gene3Di | 3.40.850.10, 1 hit |
InterProi | View protein in InterPro IPR025901, Kinesin-assoc_MT-bd_dom IPR019821, Kinesin_motor_CS IPR001752, Kinesin_motor_dom IPR036961, Kinesin_motor_dom_sf IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00225, Kinesin, 1 hit PF13931, Microtub_bind, 1 hit |
PRINTSi | PR00380, KINESINHEAVY |
SMARTi | View protein in SMART SM00129, KISc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00411, KINESIN_MOTOR_1, 1 hit PS50067, KINESIN_MOTOR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDISGGNTSR QPQKKSNQNI QVYVRVRPLN SRERCIRSAE VVDVVGPREV
60 70 80 90 100
VTRHTLDSKL TKKFTFDRSF GPESKQCDVY SVVVSPLIEE VLNGYNCTVF
110 120 130 140 150
AYGQTGTGKT HTMVGNETAE LKSSWEDDSD IGIIPRALSH LFDELRMMEV
160 170 180 190 200
EYTMRISYLE LYNEELCDLL STDDTTKIRI FDDSTKKGSV IIQGLEEIPV
210 220 230 240 250
HSKDDVYKLL EKGKERRKTA TTLMNAQSSR SHTVFSIVVH IRENGIEGED
260 270 280 290 300
MLKIGKLNLV DLAGSENVSK AGNEKGIRVR ETVNINQSLL TLGRVITALV
310 320 330 340 350
DRAPHVPYRE SKLTRLLQES LGGRTKTSII ATISPGHKDI EETLSTLEYA
360 370 380 390 400
HRAKNIQNKP EVNQKLTKKT VLKEYTEEID KLKRDLMAAR DKNGIYLAEE
410 420 430 440 450
TYGEITLKLE SQNRELNEKM LLLKALKDEL QNKEKIFSEV SMSLVEKTQE
460 470 480 490 500
LKKTEENLLN TKGTLLLTKK VLTKTKRRYK EKKELVASHM KTEQVLTTQA
510 520 530 540 550
QEILAAADLA TDDTHQLHGT IERRRELDEK IRRSCDQFKD RMQDNLEMIG
560 570 580 590 600
GSLNLYQDQQ AALKEQLSQE MVNSSYVSQR LALNSSKSIE MLKEMCAQSL
610 620 630 640 650
QDQTNLHNKL IGEVMKISDQ HSQAFVAKLM EQMQQQQLLM SKEIQTNLQV
660 670 680 690 700
IEENNQRHKA MLDSMQEKFA TIIDSSLQSV EEHAKQMHKK LEQLGAMSLP
710 720 730 740 750
DAEELQNLQE ELANERALAQ QEDALLESMM MQMEQIKNLR SKNSISMSVH
760 770 780 790 800
LNKMEESRLT RNHRIDDIKS GIQDYQKLGI EASQSAQAEL TSQMEAGMLC
810 820 830 840 850
LDQGVANCSM LQVHMKNLNQ KYEKETNENV GSVRVHHNQV EIICQESKQQ
860 870 880 890 900
LEAVQEKTEV NLEQMVDARQ QLITEDRQRF IGHATVATDL VQESNRQFSE
910 920 930 940 950
HAEHQRQQLQ ICEQELVRFQ QSELKTYAPT GTTPSKRDFV YPRTLVATSP
960 970 980 990 1000
HQEIVRRYRQ ELDWSDLDTT ATIDECSEGE HDVSMHSVQE LSETETIMNS
1010 1020 1030 1040 1050
TPIEPVDGVT VKRGCGTTRN SNSNALKPPV ATGGKRSSSL SRSLTPSKTS
1060
PRGSPAFVRH NKENVA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 962 | L → Q in AAA03718 (PubMed:8227131).Curated | 1 | |
Sequence conflicti | 983 | V → D in AAA03718 (PubMed:8227131).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U01842 mRNA Translation: AAA03718.1 AE014296 Genomic DNA Translation: AAF47458.2 AY069442 mRNA Translation: AAL39587.1 M74428 Genomic DNA Translation: AAA28655.1 |
PIRi | A48669 |
RefSeqi | NP_476818.1, NM_057470.5 |
Genome annotation databases
EnsemblMetazoai | FBtr0072733; FBpp0072616; FBgn0004378 |
GeneIDi | 38135 |
KEGGi | dme:Dmel_CG9191 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U01842 mRNA Translation: AAA03718.1 AE014296 Genomic DNA Translation: AAF47458.2 AY069442 mRNA Translation: AAL39587.1 M74428 Genomic DNA Translation: AAA28655.1 |
PIRi | A48669 |
RefSeqi | NP_476818.1, NM_057470.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2WBE | electron microscopy | 9.40 | C | 1-368 | [»] | |
4PXT | X-ray | 2.90 | A/B | 634-837 | [»] | |
4PXU | X-ray | 2.60 | A/B | 634-837 | [»] | |
6VPO | electron microscopy | 4.40 | C | 1-369 | [»] | |
6VPP | electron microscopy | 4.40 | C | 1-369 | [»] | |
AlphaFoldDBi | P46863 | |||||
SMRi | P46863 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 63681, 15 interactors |
DIPi | DIP-22047N |
IntActi | P46863, 9 interactors |
MINTi | P46863 |
STRINGi | 7227.FBpp0072616 |
Chemistry databases
ChEMBLi | CHEMBL1795160 |
PTM databases
iPTMneti | P46863 |
Proteomic databases
PaxDbi | P46863 |
PRIDEi | P46863 |
Protocols and materials databases
DNASUi | 38135 |
Genome annotation databases
EnsemblMetazoai | FBtr0072733; FBpp0072616; FBgn0004378 |
GeneIDi | 38135 |
KEGGi | dme:Dmel_CG9191 |
Organism-specific databases
CTDi | 38135 |
FlyBasei | FBgn0004378, Klp61F |
VEuPathDBi | VectorBase:FBgn0004378 |
Phylogenomic databases
eggNOGi | KOG0243, Eukaryota |
GeneTreei | ENSGT00940000155921 |
HOGENOMi | CLU_001485_33_4_1 |
InParanoidi | P46863 |
OMAi | PGHKDIE |
OrthoDBi | 179272at2759 |
PhylomeDBi | P46863 |
Enzyme and pathway databases
Reactomei | R-DME-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-DME-983189, Kinesins |
SignaLinki | P46863 |
Miscellaneous databases
BioGRID-ORCSi | 38135, 1 hit in 1 CRISPR screen |
EvolutionaryTracei | P46863 |
GenomeRNAii | 38135 |
PROi | PR:P46863 |
Gene expression databases
Bgeei | FBgn0004378, Expressed in embryo and 41 other tissues |
ExpressionAtlasi | P46863, baseline and differential |
Genevisiblei | P46863, DM |
Family and domain databases
Gene3Di | 3.40.850.10, 1 hit |
InterProi | View protein in InterPro IPR025901, Kinesin-assoc_MT-bd_dom IPR019821, Kinesin_motor_CS IPR001752, Kinesin_motor_dom IPR036961, Kinesin_motor_dom_sf IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00225, Kinesin, 1 hit PF13931, Microtub_bind, 1 hit |
PRINTSi | PR00380, KINESINHEAVY |
SMARTi | View protein in SMART SM00129, KISc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00411, KINESIN_MOTOR_1, 1 hit PS50067, KINESIN_MOTOR_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KL61_DROME | |
Accessioni | P46863Primary (citable) accession number: P46863 Secondary accession number(s): Q8T0A6, Q9W0I8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 28, 2002 | |
Last modified: | May 25, 2022 | |
This is version 178 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families