UniProtKB - P46854 (AAAT_ECOLI)
Protein
L-amino acid N-acetyltransferase AaaT
Gene
aaaT
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the N-acetylation of L-phenylalanine and L-methionine using acetyl-CoA as acetyl donor in vitro. Cannot accept L-tyrosine as substrate and propionyl-CoA, succinyl-CoA or (S)-methylmalonyl-CoA as acyl donors (PubMed:27941785). Is also able to acetylate and thus detoxify several nonhydrolyzable aminoacyl adenylates, but not the processed form of the peptide-nucleotide antibiotic microcin C (McC). When overproduced, provides complete resistance to leucyl sulfamoyl adenylate (LSA) and partial resistance to alanyl sulfamoyl adenylate (ASA) and phenylalanyl sulfamoyl adenylate (FSA). Therefore, may protect bacteria from various toxic aminoacyl nucleotides, either exogenous or those generated inside the cell during normal metabolism (PubMed:25002546).2 Publications
Catalytic activityi
- EC:2.3.1.531 Publication
GO - Molecular functioni
- N-acetyltransferase activity Source: EcoCyc
- phenylalanine N-acetyltransferase activity Source: UniProtKB-EC
- transferase activity, transferring acyl groups other than amino-acyl groups Source: GO_Central
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | EcoCyc:G7758-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:aaaT1 Publication Synonyms:yhhYImported Ordered Locus Names:b3441, JW3405 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074613 | 1 – 162 | L-amino acid N-acetyltransferase AaaTAdd BLAST | 162 |
Proteomic databases
jPOSTi | P46854 |
PaxDbi | P46854 |
PRIDEi | P46854 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259623, 13 interactors |
IntActi | P46854, 4 interactors |
STRINGi | 511145.b3441 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 162 | N-acetyltransferasePROSITE-ProRule annotationAdd BLAST | 159 |
Sequence similaritiesi
Belongs to the acetyltransferase family.Curated
Phylogenomic databases
eggNOGi | COG1247, Bacteria |
HOGENOMi | CLU_013985_19_8_6 |
InParanoidi | P46854 |
PhylomeDBi | P46854 |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
i Sequence
Sequence statusi: Complete.
P46854-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEIVIRHAE TRDYEAIRQI HAQPEVYCNT LQVPHPSDHM WQERLADRPG
60 70 80 90 100
IKQLVACIDG DVVGHLTIDV QQRPRRSHVA DFGICVDSRW KNRGVASALM
110 120 130 140 150
REMIEMCDNW LRVDRIELTV FVDNAPAIKV YKKYGFEIEG TGKKYALRNG
160
EYVDAYYMAR VK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58239.1 U00096 Genomic DNA Translation: AAC76466.1 AP009048 Genomic DNA Translation: BAE77852.1 |
PIRi | D65140 |
RefSeqi | NP_417898.1, NC_000913.3 WP_001295206.1, NZ_SSZK01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC76466; AAC76466; b3441 BAE77852; BAE77852; BAE77852 |
GeneIDi | 57729294 947943 |
KEGGi | ecj:JW3405 eco:b3441 |
PATRICi | fig|511145.12.peg.3538 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58239.1 U00096 Genomic DNA Translation: AAC76466.1 AP009048 Genomic DNA Translation: BAE77852.1 |
PIRi | D65140 |
RefSeqi | NP_417898.1, NC_000913.3 WP_001295206.1, NZ_SSZK01000008.1 |
3D structure databases
SMRi | P46854 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259623, 13 interactors |
IntActi | P46854, 4 interactors |
STRINGi | 511145.b3441 |
Proteomic databases
jPOSTi | P46854 |
PaxDbi | P46854 |
PRIDEi | P46854 |
Genome annotation databases
EnsemblBacteriai | AAC76466; AAC76466; b3441 BAE77852; BAE77852; BAE77852 |
GeneIDi | 57729294 947943 |
KEGGi | ecj:JW3405 eco:b3441 |
PATRICi | fig|511145.12.peg.3538 |
Organism-specific databases
EchoBASEi | EB2779 |
Phylogenomic databases
eggNOGi | COG1247, Bacteria |
HOGENOMi | CLU_013985_19_8_6 |
InParanoidi | P46854 |
PhylomeDBi | P46854 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7758-MONOMER |
Miscellaneous databases
PROi | PR:P46854 |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AAAT_ECOLI | |
Accessioni | P46854Primary (citable) accession number: P46854 Secondary accession number(s): Q2M7A4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families