Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cystathionine beta-synthase

Gene

cysB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-serine + L-homocysteine = L-cystathionine + H2O.

Cofactori

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Cystathionine beta-synthase (cysB)
  2. Cystathionine gamma-lyase (cysA)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei103Pyridoxal phosphateBy similarity1
Binding sitei302Pyridoxal phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-DDI-1614603 Cysteine formation from homocysteine
UniPathwayiUPA00136; UER00201

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine beta-synthase (EC:4.2.1.22)
Alternative name(s):
Beta-thionase
Serine sulfhydrase
Gene namesi
Name:cysB
ORF Names:DDB_G0267386
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0267386 cysB

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001671321 – 498Cystathionine beta-synthaseAdd BLAST498

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP46794
PRIDEiP46794

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0191292

Structurei

3D structure databases

ProteinModelPortaliP46794
SMRiP46794
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini374 – 430CBS 1PROSITE-ProRule annotationAdd BLAST57
Domaini435 – 497CBS 2PROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 214Pyridoxal phosphate bindingBy similarity5

Sequence similaritiesi

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiKOG1252 Eukaryota
COG0031 LUCA
InParanoidiP46794
KOiK01697
OMAiSYLSKFA
PhylomeDBiP46794

Family and domain databases

InterProiView protein in InterPro
IPR000644 CBS_dom
IPR005857 Cysta_beta_synth
IPR001216 P-phosphate_BS
IPR001926 PLP-dep
IPR036052 Trypto_synt_PLP_dependent
PfamiView protein in Pfam
PF00571 CBS, 2 hits
PF00291 PALP, 1 hit
SMARTiView protein in SMART
SM00116 CBS, 2 hits
SUPFAMiSSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR01137 cysta_beta, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 2 hits
PS00901 CYS_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P46794-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPEGPSKC TWTPNTTENT PHTTRRTPKK LIMDNILDNI GGTPLVRVNK
60 70 80 90 100
VSSDLECELV AKCEFFNAGG SVKDRIGHRM IVDAEESGRI KKGDTLIEPT
110 120 130 140 150
SGNTGIGLAL TAAIKGYKMI ITLPEKMSQE KVDVLKALGA EIIRTPTEAA
160 170 180 190 200
FDAPESHIGV AKKLNSEIPN SHILDQYGNP SNPLAHYDGT AEELLEQCEG
210 220 230 240 250
KIDMIVCTAG TGGTITGIAR KIKERLPNCI VVGVDPHGSI LAQPESLNNT
260 270 280 290 300
NKSYKIEGIG YDFIPNVLER KLVDQWIKTD DKESFIMARR LIKEEGLLCG
310 320 330 340 350
GSSGSAMVGA LLAAKQLKKG QRCVVLLADS IRNYMTKHLN DDWLVDNGFV
360 370 380 390 400
DPEYKTKDQQ EEEKYHGATV KDLTLPKPIT ISATTTCAAA VQLLQQYGFD
410 420 430 440 450
QLPVVSESKK VLGQLTLGNL LSHIASKKAV PTDAVSKVMF RFTKNEKYIP
460 470 480 490
ITQSTSLATL SKFFENHSSA IVTENDEIIS IVTKIDLLTY LMKSQQKN
Length:498
Mass (Da):54,458
Last modified:December 4, 2007 - v2
Checksum:i31FD69EFB68B6432
GO

Sequence cautioni

The sequence AAA70099 differs from that shown. Reason: Frameshift at positions 413 and 424.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti140A → G in AAA70099 (Ref. 1) Curated1
Sequence conflicti300G → A in AAA70099 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27536 mRNA Translation: AAA70099.1 Frameshift.
AAFI02000003 Genomic DNA Translation: EAL73145.1
RefSeqiXP_647501.1, XM_642409.1

Genome annotation databases

EnsemblProtistsiEAL73145; EAL73145; DDB_G0267386
GeneIDi8616308
KEGGiddi:DDB_G0267386

Similar proteinsi

Entry informationi

Entry nameiCBS_DICDI
AccessioniPrimary (citable) accession number: P46794
Secondary accession number(s): Q55FN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 4, 2007
Last modified: May 23, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health