Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

60S ribosomal protein L28

Gene

RPL28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the large ribosomal subunit.1 Publication

GO - Molecular functioni

  • RNA binding Source: UniProtKB
  • structural constituent of ribosome Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

ReactomeiR-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-156902 Peptide chain elongation
R-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane
R-HSA-192823 Viral mRNA Translation
R-HSA-2408557 Selenocysteine synthesis
R-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-HSA-72764 Eukaryotic Translation Termination
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L28
Alternative name(s):
Large ribosomal subunit protein eL281 Publication
Gene namesi
Name:RPL28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000108107.12
HGNCiHGNC:10330 RPL28
MIMi603638 gene
neXtProtiNX_P46779

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi6158
OpenTargetsiENSG00000108107
PharmGKBiPA34710

Polymorphism and mutation databases

BioMutaiRPL28

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00001223892 – 13760S ribosomal protein L28Add BLAST136

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources2 Publications1
Cross-linki58Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki65Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei115PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP46779
MaxQBiP46779
PaxDbiP46779
PeptideAtlasiP46779
PRIDEiP46779
ProteomicsDBi55761
55762 [P46779-2]
TopDownProteomicsiP46779-1 [P46779-1]

PTM databases

iPTMnetiP46779
PhosphoSitePlusiP46779
SwissPalmiP46779

Miscellaneous databases

PMAP-CutDBiP46779

Expressioni

Gene expression databases

BgeeiENSG00000108107 Expressed in 231 organ(s), highest expression level in blood
CleanExiHS_RPL28
ExpressionAtlasiP46779 baseline and differential
GenevisibleiP46779 HS

Organism-specific databases

HPAiHPA050459

Interactioni

Subunit structurei

Component of the large ribosomal subunit.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi112077, 167 interactors
CORUMiP46779
IntActiP46779, 43 interactors
MINTiP46779
STRINGi9606.ENSP00000401450

Structurei

3D structure databases

ProteinModelPortaliP46779
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3412 Eukaryota
ENOG4111T32 LUCA
GeneTreeiENSGT00390000008732
HOGENOMiHOG000200513
HOVERGENiHBG000701
InParanoidiP46779
KOiK02903
OMAiWMIIRNN
PhylomeDBiP46779
TreeFamiTF300173

Family and domain databases

InterProiView protein in InterPro
IPR029004 L28e/Mak16
IPR002672 Ribosomal_L28e
PANTHERiPTHR10544 PTHR10544, 1 hit
PfamiView protein in Pfam
PF01778 Ribosomal_L28e, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P46779-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG
60 70 80 90 100
VEPAADGKGV VVVIKRRSGQ RKPATSYVRT TINKNARATL SSIRHMIRKN
110 120 130
KYRPDLRMAA IRRASAILRS QKPVMVKRKR TRPTKSS
Length:137
Mass (Da):15,748
Last modified:January 23, 2007 - v3
Checksum:i44DCDE45473D7C7B
GO
Isoform 2 (identifier: P46779-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     109-137: AAIRRASAILRSQKPVMVKRKRTRPTKSS → VSWGLGIRLG...PRGRLVECMG

Note: No experimental confirmation available.
Show »
Length:163
Mass (Da):18,430
Checksum:i1C9BD1FA0A0E14EE
GO
Isoform 3 (identifier: P46779-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     109-137: AAIRRASAILRSQKPVMVKRKRTRPTKSS → DMLASTGSGL...QNLLDCAHKS

Note: No experimental confirmation available.
Show »
Length:169
Mass (Da):19,148
Checksum:iD4D6E5C7F4116D16
GO
Isoform 4 (identifier: P46779-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     69-137: GQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMAAIRRASAILRSQKPVMVKRKRTRPTKSS → E

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:69
Mass (Da):7,888
Checksum:iEDE20EE54DFE31FE
GO
Isoform 5 (identifier: P46779-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     70-137: QRKPATSYVR...RKRTRPTKSS → EFCLVWARERPLSRVWEL

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:87
Mass (Da):10,088
Checksum:i791604CE95F509E2
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YKD8H0YKD8_HUMAN
60S ribosomal protein L28
RPL28
170Annotation score:
H0YMF4H0YMF4_HUMAN
60S ribosomal protein L28
RPL28
112Annotation score:
H0YLP6H0YLP6_HUMAN
60S ribosomal protein L28
RPL28
89Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84K → R in AAH10182 (PubMed:15489334).Curated1
Sequence conflicti120S → T in AAA85657 (PubMed:7772601).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03446066R → L. Corresponds to variant dbSNP:rs13502Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04702669 – 137GQRKP…PTKSS → E in isoform 4. CuratedAdd BLAST69
Alternative sequenceiVSP_04702770 – 137QRKPA…PTKSS → EFCLVWARERPLSRVWEL in isoform 5. CuratedAdd BLAST68
Alternative sequenceiVSP_043026109 – 137AAIRR…PTKSS → VSWGLGIRLGETGQCCGEGP PTTGCNMGWRGMDSCFQPTP HTQHWPRGRLVECMG in isoform 2. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_046173109 – 137AAIRR…PTKSS → DMLASTGSGLCCSVAVQPWA SSSTSLCLRTLICNMRVDRP YYSGLMRRLNVQNLLDCAHK S in isoform 3. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14969 mRNA Translation: AAA85657.1
CR457024 mRNA Translation: CAG33305.1
AK311760 mRNA Translation: BAG34703.1
AK293736 mRNA Translation: BAG57160.1
AC020922 Genomic DNA No translation available.
CH471135 Genomic DNA Translation: EAW72374.1
CH471135 Genomic DNA Translation: EAW72375.1
CH471135 Genomic DNA Translation: EAW72376.1
BC010173 mRNA Translation: AAH10173.1
BC010182 mRNA Translation: AAH10182.1
BC011582 mRNA Translation: AAH11582.1
BG777550 mRNA No translation available.
CCDSiCCDS12924.1 [P46779-1]
CCDS46189.1 [P46779-3]
CCDS46190.1 [P46779-2]
CCDS46191.1 [P46779-4]
CCDS46192.1 [P46779-5]
PIRiS55915
RefSeqiNP_000982.2, NM_000991.4 [P46779-1]
NP_001129606.1, NM_001136134.1 [P46779-2]
NP_001129607.1, NM_001136135.1 [P46779-3]
NP_001129608.1, NM_001136136.1 [P46779-5]
NP_001129609.1, NM_001136137.1 [P46779-4]
UniGeneiHs.652114

Genome annotation databases

EnsembliENST00000344063; ENSP00000342787; ENSG00000108107 [P46779-1]
ENST00000428193; ENSP00000391665; ENSG00000108107 [P46779-4]
ENST00000431533; ENSP00000400596; ENSG00000108107 [P46779-5]
ENST00000558815; ENSP00000452909; ENSG00000108107 [P46779-3]
ENST00000559463; ENSP00000453319; ENSG00000108107 [P46779-1]
ENST00000560583; ENSP00000453029; ENSG00000108107 [P46779-2]
GeneIDi6158
KEGGihsa:6158
UCSCiuc002qkv.4 human [P46779-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14969 mRNA Translation: AAA85657.1
CR457024 mRNA Translation: CAG33305.1
AK311760 mRNA Translation: BAG34703.1
AK293736 mRNA Translation: BAG57160.1
AC020922 Genomic DNA No translation available.
CH471135 Genomic DNA Translation: EAW72374.1
CH471135 Genomic DNA Translation: EAW72375.1
CH471135 Genomic DNA Translation: EAW72376.1
BC010173 mRNA Translation: AAH10173.1
BC010182 mRNA Translation: AAH10182.1
BC011582 mRNA Translation: AAH11582.1
BG777550 mRNA No translation available.
CCDSiCCDS12924.1 [P46779-1]
CCDS46189.1 [P46779-3]
CCDS46190.1 [P46779-2]
CCDS46191.1 [P46779-4]
CCDS46192.1 [P46779-5]
PIRiS55915
RefSeqiNP_000982.2, NM_000991.4 [P46779-1]
NP_001129606.1, NM_001136134.1 [P46779-2]
NP_001129607.1, NM_001136135.1 [P46779-3]
NP_001129608.1, NM_001136136.1 [P46779-5]
NP_001129609.1, NM_001136137.1 [P46779-4]
UniGeneiHs.652114

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UG0electron microscopy-Lr1-137[»]
4V6Xelectron microscopy5.00Cr1-137[»]
5AJ0electron microscopy3.50At1-137[»]
5LKSelectron microscopy3.60Lr1-137[»]
5T2Celectron microscopy3.60k1-137[»]
6EK0electron microscopy2.90Lr1-137[»]
ProteinModelPortaliP46779
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112077, 167 interactors
CORUMiP46779
IntActiP46779, 43 interactors
MINTiP46779
STRINGi9606.ENSP00000401450

PTM databases

iPTMnetiP46779
PhosphoSitePlusiP46779
SwissPalmiP46779

Polymorphism and mutation databases

BioMutaiRPL28

Proteomic databases

EPDiP46779
MaxQBiP46779
PaxDbiP46779
PeptideAtlasiP46779
PRIDEiP46779
ProteomicsDBi55761
55762 [P46779-2]
TopDownProteomicsiP46779-1 [P46779-1]

Protocols and materials databases

DNASUi6158
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000344063; ENSP00000342787; ENSG00000108107 [P46779-1]
ENST00000428193; ENSP00000391665; ENSG00000108107 [P46779-4]
ENST00000431533; ENSP00000400596; ENSG00000108107 [P46779-5]
ENST00000558815; ENSP00000452909; ENSG00000108107 [P46779-3]
ENST00000559463; ENSP00000453319; ENSG00000108107 [P46779-1]
ENST00000560583; ENSP00000453029; ENSG00000108107 [P46779-2]
GeneIDi6158
KEGGihsa:6158
UCSCiuc002qkv.4 human [P46779-1]

Organism-specific databases

CTDi6158
DisGeNETi6158
EuPathDBiHostDB:ENSG00000108107.12
GeneCardsiRPL28
HGNCiHGNC:10330 RPL28
HPAiHPA050459
MIMi603638 gene
neXtProtiNX_P46779
OpenTargetsiENSG00000108107
PharmGKBiPA34710
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3412 Eukaryota
ENOG4111T32 LUCA
GeneTreeiENSGT00390000008732
HOGENOMiHOG000200513
HOVERGENiHBG000701
InParanoidiP46779
KOiK02903
OMAiWMIIRNN
PhylomeDBiP46779
TreeFamiTF300173

Enzyme and pathway databases

ReactomeiR-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-156902 Peptide chain elongation
R-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane
R-HSA-192823 Viral mRNA Translation
R-HSA-2408557 Selenocysteine synthesis
R-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-HSA-72764 Eukaryotic Translation Termination
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Miscellaneous databases

ChiTaRSiRPL28 human
GeneWikii60S_ribosomal_protein_L28
GenomeRNAii6158
PMAP-CutDBiP46779
PROiPR:P46779
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108107 Expressed in 231 organ(s), highest expression level in blood
CleanExiHS_RPL28
ExpressionAtlasiP46779 baseline and differential
GenevisibleiP46779 HS

Family and domain databases

InterProiView protein in InterPro
IPR029004 L28e/Mak16
IPR002672 Ribosomal_L28e
PANTHERiPTHR10544 PTHR10544, 1 hit
PfamiView protein in Pfam
PF01778 Ribosomal_L28e, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRL28_HUMAN
AccessioniPrimary (citable) accession number: P46779
Secondary accession number(s): B2R4A6
, B4DEP9, C9JB50, E9PB24, G5E9L2, Q6IAY0, Q96FX1, Q9BWQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 180 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Ribosomal proteins
    Ribosomal proteins families and list of entries
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  7. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again