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Protein

60S ribosomal protein L5

Gene

RPL5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs (PubMed:12962325, PubMed:19061985, PubMed:24120868, PubMed:23636399). It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (PubMed:24120868).4 Publications

GO - Molecular functioni

  • 5S rRNA binding Source: CAFA
  • mRNA 3'-UTR binding Source: CAFA
  • mRNA 5'-UTR binding Source: CAFA
  • RNA binding Source: UniProtKB
  • structural constituent of ribosome Source: ProtInc
  • ubiquitin ligase inhibitor activity Source: CAFA
  • ubiquitin protein ligase binding Source: CAFA

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-156902 Peptide chain elongation
R-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane
R-HSA-192823 Viral mRNA Translation
R-HSA-2408557 Selenocysteine synthesis
R-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-HSA-72764 Eukaryotic Translation Termination
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)
SIGNORiP46777

Protein family/group databases

MoonProtiP46777

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L5
Alternative name(s):
Large ribosomal subunit protein uL181 Publication
Gene namesi
Name:RPL5
ORF Names:MSTP030
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000122406.12
HGNCiHGNC:10360 RPL5
MIMi603634 gene
neXtProtiNX_P46777

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Diamond-Blackfan anemia 6 (DBA6)2 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
See also OMIM:612561
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055450140G → S in DBA6. 1 PublicationCorresponds to variant dbSNP:rs121434406EnsemblClinVar.1
Natural variantiVAR_055451285A → V in DBA6. 1 Publication1

Keywords - Diseasei

Diamond-Blackfan anemia, Disease mutation

Organism-specific databases

DisGeNETi6125
GeneReviewsiRPL5
MalaCardsiRPL5
MIMi612561 phenotype
OpenTargetsiENSG00000122406
Orphaneti124 Blackfan-Diamond anemia
PharmGKBiPA34755

Polymorphism and mutation databases

BioMutaiRPL5
DMDMi81175191

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001314312 – 29760S ribosomal protein L5Add BLAST296

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineCombined sources1
Modified residuei5N6-acetyllysineCombined sources1
Modified residuei48N6-acetyllysineCombined sources1
Modified residuei185PhosphoserineCombined sources1
Modified residuei220N6-acetyllysine; alternateBy similarity1
Cross-linki220Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki220Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei232PhosphothreonineCombined sources1
Modified residuei272PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP46777
MaxQBiP46777
PaxDbiP46777
PeptideAtlasiP46777
PRIDEiP46777
ProteomicsDBi55759
TopDownProteomicsiP46777

PTM databases

iPTMnetiP46777
PhosphoSitePlusiP46777
SwissPalmiP46777

Expressioni

Gene expression databases

BgeeiENSG00000122406 Expressed in 228 organ(s), highest expression level in germinal epithelium of ovary
CleanExiHS_RPL5
ExpressionAtlasiP46777 baseline and differential
GenevisibleiP46777 HS

Organism-specific databases

HPAiHPA043717
HPA054444

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Part of a LSU subcomplex, the 5S RNP which is composed of the 5S RNA, RPL5 and RPL11 (PubMed:24120868). Interacts with isoform 1 of NVL in an ATP-dependent manner (PubMed:15469983). Interacts with RRP1B (PubMed:20926688).3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112045, 240 interactors
CORUMiP46777
DIPiDIP-31152N
IntActiP46777, 45 interactors
MINTiP46777
STRINGi9606.ENSP00000359345

Structurei

3D structure databases

ProteinModelPortaliP46777
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0875 Eukaryota
COG0256 LUCA
GeneTreeiENSGT00390000008456
HOVERGENiHBG009347
InParanoidiP46777
KOiK02932
OMAiVAEYMES
OrthoDBiEOG091G0GZO
PhylomeDBiP46777
TreeFamiTF300044

Family and domain databases

HAMAPiMF_01337_A Ribosomal_L18_A, 1 hit
InterProiView protein in InterPro
IPR005485 Rbsml_L5_euk/L18_arc
IPR025607 Rbsml_L5e/L18P_C
PANTHERiPTHR23410 PTHR23410, 1 hit
PfamiView protein in Pfam
PF14204 Ribosomal_L18_c, 1 hit
PF17144 Ribosomal_L5e, 1 hit
PRINTSiPR00058 RIBOSOMALL5

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

P46777-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR
60 70 80 90 100
MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVKV GLTNYAAAYC
110 120 130 140 150
TGLLLARRLL NRFGMDKIYE GQVEVTGDEY NVESIDGQPG AFTCYLDAGL
160 170 180 190 200
ARTTTGNKVF GALKGAVDGG LSIPHSTKRF PGYDSESKEF NAEVHRKHIM
210 220 230 240 250
GQNVADYMRY LMEEDEDAYK KQFSQYIKNS VTPDMMEEMY KKAHAAIREN
260 270 280 290
PVYEKKPKKE VKKKRWNRPK MSLAQKKDRV AQKKASFLRA QERAAES
Length:297
Mass (Da):34,363
Last modified:January 23, 2007 - v3
Checksum:i07CA9FBA842BD2A3
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5T7N0Q5T7N0_HUMAN
60S ribosomal protein L5
RPL5
130Annotation score:
R4GNJ2R4GNJ2_HUMAN
60S ribosomal protein L5
RPL5
63Annotation score:
A0A2R8Y4A2A0A2R8Y4A2_HUMAN
60S ribosomal protein L5
RPL5
170Annotation score:
A0A2R8Y6J3A0A2R8Y6J3_HUMAN
60S ribosomal protein L5
RPL5
238Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78A → R in AAA85654 (PubMed:7772601).Curated1
Sequence conflicti176S → R (PubMed:7937132).Curated1
Sequence conflicti264K → E in BAD96324 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055450140G → S in DBA6. 1 PublicationCorresponds to variant dbSNP:rs121434406EnsemblClinVar.1
Natural variantiVAR_052009210Y → C1 PublicationCorresponds to variant dbSNP:rs11540832EnsemblClinVar.1
Natural variantiVAR_055451285A → V in DBA6. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14966 mRNA Translation: AAA85654.1
AF113210 mRNA Translation: AAG39281.1
AK222604 mRNA Translation: BAD96324.1
AL162740 Genomic DNA No translation available.
BC109370 mRNA Translation: AAI09371.1
U76609 mRNA Translation: AAB18361.1
Z35312 Genomic DNA No translation available.
CCDSiCCDS741.1
PIRiS55912
RefSeqiNP_000960.2, NM_000969.3
UniGeneiHs.532359
Hs.568386

Genome annotation databases

EnsembliENST00000370321; ENSP00000359345; ENSG00000122406
ENST00000644759; ENSP00000495549; ENSG00000122406
GeneIDi6125
KEGGihsa:6125
UCSCiuc001doz.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Diamond-Blackfan Anemia mutation database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14966 mRNA Translation: AAA85654.1
AF113210 mRNA Translation: AAG39281.1
AK222604 mRNA Translation: BAD96324.1
AL162740 Genomic DNA No translation available.
BC109370 mRNA Translation: AAI09371.1
U76609 mRNA Translation: AAB18361.1
Z35312 Genomic DNA No translation available.
CCDSiCCDS741.1
PIRiS55912
RefSeqiNP_000960.2, NM_000969.3
UniGeneiHs.532359
Hs.568386

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UG0electron microscopy-LD1-297[»]
4V6Xelectron microscopy5.00CD1-297[»]
5AJ0electron microscopy3.50AD1-297[»]
5LKSelectron microscopy3.60LD1-297[»]
5T2Celectron microscopy3.60G1-297[»]
6EK0electron microscopy2.90LD1-297[»]
ProteinModelPortaliP46777
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112045, 240 interactors
CORUMiP46777
DIPiDIP-31152N
IntActiP46777, 45 interactors
MINTiP46777
STRINGi9606.ENSP00000359345

Protein family/group databases

MoonProtiP46777

PTM databases

iPTMnetiP46777
PhosphoSitePlusiP46777
SwissPalmiP46777

Polymorphism and mutation databases

BioMutaiRPL5
DMDMi81175191

Proteomic databases

EPDiP46777
MaxQBiP46777
PaxDbiP46777
PeptideAtlasiP46777
PRIDEiP46777
ProteomicsDBi55759
TopDownProteomicsiP46777

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370321; ENSP00000359345; ENSG00000122406
ENST00000644759; ENSP00000495549; ENSG00000122406
GeneIDi6125
KEGGihsa:6125
UCSCiuc001doz.4 human

Organism-specific databases

CTDi6125
DisGeNETi6125
EuPathDBiHostDB:ENSG00000122406.12
GeneCardsiRPL5
GeneReviewsiRPL5
HGNCiHGNC:10360 RPL5
HPAiHPA043717
HPA054444
MalaCardsiRPL5
MIMi603634 gene
612561 phenotype
neXtProtiNX_P46777
OpenTargetsiENSG00000122406
Orphaneti124 Blackfan-Diamond anemia
PharmGKBiPA34755
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0875 Eukaryota
COG0256 LUCA
GeneTreeiENSGT00390000008456
HOVERGENiHBG009347
InParanoidiP46777
KOiK02932
OMAiVAEYMES
OrthoDBiEOG091G0GZO
PhylomeDBiP46777
TreeFamiTF300044

Enzyme and pathway databases

ReactomeiR-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-156902 Peptide chain elongation
R-HSA-1799339 SRP-dependent cotranslational protein targeting to membrane
R-HSA-192823 Viral mRNA Translation
R-HSA-2408557 Selenocysteine synthesis
R-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-HSA-72764 Eukaryotic Translation Termination
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)
SIGNORiP46777

Miscellaneous databases

ChiTaRSiRPL5 human
GeneWikiiRibosomal_protein_L5
GenomeRNAii6125
PROiPR:P46777
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000122406 Expressed in 228 organ(s), highest expression level in germinal epithelium of ovary
CleanExiHS_RPL5
ExpressionAtlasiP46777 baseline and differential
GenevisibleiP46777 HS

Family and domain databases

HAMAPiMF_01337_A Ribosomal_L18_A, 1 hit
InterProiView protein in InterPro
IPR005485 Rbsml_L5_euk/L18_arc
IPR025607 Rbsml_L5e/L18P_C
PANTHERiPTHR23410 PTHR23410, 1 hit
PfamiView protein in Pfam
PF14204 Ribosomal_L18_c, 1 hit
PF17144 Ribosomal_L5e, 1 hit
PRINTSiPR00058 RIBOSOMALL5
ProtoNetiSearch...

Entry informationi

Entry nameiRL5_HUMAN
AccessioniPrimary (citable) accession number: P46777
Secondary accession number(s): Q32LZ3, Q53HH6, Q9H3F4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 183 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Ribosomal proteins
    Ribosomal proteins families and list of entries
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  7. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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