Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Porphobilinogen deaminase (hemC)
  3. no protein annotated in this organism
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei202Schiff-base intermediate with substrateBy similarity1
Binding sitei212Substrate 1By similarity1
Binding sitei223Substrate 1By similarity1
Metal bindingi239MagnesiumBy similarity1
Active sitei254Schiff-base intermediate with substrateBy similarity1
Binding sitei280Substrate 2By similarity1
Binding sitei319Substrate 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processHeme biosynthesis, Porphyrin biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-2653-MONOMER
UniPathwayiUPA00251; UER00318

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
Ordered Locus Names:ML2419
ORF Names:B2168_C3_264
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML2419

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001405041 – 329Delta-aminolevulinic acid dehydrataseAdd BLAST329

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi272631.ML2419

Structurei

3D structure databases

ProteinModelPortaliP46723
SMRiP46723
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Phylogenomic databases

eggNOGiENOG4105D52 Bacteria
COG0113 LUCA
HOGENOMiHOG000020323
KOiK01698
OMAiYQMDYAN
OrthoDBiPOG091H03SU

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR001731 ALAD
IPR030656 ALAD_AS
IPR013785 Aldolase_TIM
PANTHERiPTHR11458 PTHR11458, 1 hit
PfamiView protein in Pfam
PF00490 ALAD, 1 hit
PIRSFiPIRSF001415 Porphbilin_synth, 1 hit
PRINTSiPR00144 DALDHYDRTASE
SMARTiView protein in SMART
SM01004 ALAD, 1 hit
PROSITEiView protein in PROSITE
PS00169 D_ALA_DEHYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

P46723-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSGYPRHR PRRLRSTPAM RRLVAQTSLE PRNLVLPMFV ADGIDELRPI
60 70 80 90 100
ASMPGVVQHT RDSLRRAAVA AVDAGVGGLN LFGVPRDQDK DATGSAGVDP
110 120 130 140 150
DGILNVALRD LAEDLGDATV LMADTCLDEF TDHGHCGVLD GQGRVDNDAT
160 170 180 190 200
VARYVELAVA QAESGANVVG PSGMMDGQIG ALRDGLDSAG YADVAILAYA
210 220 230 240 250
AKFSSAFYGP FREAVSCSLS GDRRTYQQEP GNAREALREI KLDLDEGADI
260 270 280 290 300
IMIKPASGYL DVVATAAGVS PVPVAAYQVS GEYAMICAAA ANNWIDERAA
310 320
VLESLTSIRR AGADIVFTYW AADVACWLS
Length:329
Mass (Da):34,775
Last modified:November 1, 1995 - v1
Checksum:i1E0EBA9498B8609C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00018 Genomic DNA Translation: AAA17246.1
AL583925 Genomic DNA Translation: CAC31935.1
PIRiS72910
RefSeqiNP_302564.1, NC_002677.1
WP_010908884.1, NC_002677.1

Genome annotation databases

EnsemblBacteriaiCAC31935; CAC31935; CAC31935
GeneIDi908581
KEGGimle:ML2419
PATRICifig|272631.5.peg.4656

Similar proteinsi

Entry informationi

Entry nameiHEM2_MYCLE
AccessioniPrimary (citable) accession number: P46723
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health