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Protein

Radiation-inducible immediate-early gene IEX-1

Gene

IER3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.3 Publications

GO - Biological processi

  • anatomical structure morphogenesis Source: ProtInc
  • apoptotic process Source: ProtInc
  • negative regulation of apoptotic process Source: ProtInc
  • regulation of phosphatidylinositol 3-kinase signaling Source: Reactome
  • regulation of response to DNA damage stimulus Source: MGI

Enzyme and pathway databases

ReactomeiR-HSA-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
SIGNORiP46695

Names & Taxonomyi

Protein namesi
Recommended name:
Radiation-inducible immediate-early gene IEX-1
Alternative name(s):
Differentiation-dependent gene 2 protein
Short name:
Protein DIF-2
Immediate early protein GLY96
Immediate early response 3 protein
PACAP-responsive gene 1 protein
Short name:
Protein PRG1
Gene namesi
Name:IER3
Synonyms:DIF2, IEX1, PRG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000137331.11
HGNCiHGNC:5392 IER3
MIMi602996 gene
neXtProtiNX_P46695

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 82CytoplasmicSequence analysisAdd BLAST82
Transmembranei83 – 99Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini100 – 156ExtracellularSequence analysisAdd BLAST57

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8870
OpenTargetsiENSG00000137331
PharmGKBiPA29639

Polymorphism and mutation databases

BioMutaiIER3
DMDMi317373569

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000841591 – 156Radiation-inducible immediate-early gene IEX-1Add BLAST156

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18Phosphothreonine; by MAPK11 Publication1
Modified residuei31PhosphoserineCombined sources1
Modified residuei123Phosphothreonine; by MAPK11 Publication1
Modified residuei126Phosphoserine; by MAPK11 Publication1
Glycosylationi133N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Phosphorylated at Thr-18, Thr-123 and Ser-126 by MAPK1/ERK2 and probably MAPK3/ERK1. Upon phosphorylation by MAPK1/ERK2 and MAPK3/ERK1, acquires the ability to inhibit cell death induced by various stimuli.1 Publication
Glycosylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP46695
PeptideAtlasiP46695
PRIDEiP46695
ProteomicsDBi55747

PTM databases

iPTMnetiP46695
PhosphoSitePlusiP46695

Expressioni

Inductioni

By radiation, 12-O-tetradecanoyl phorbol-13 acetate (TPA), okadaic acid, TNF and NUPR1.1 Publication

Gene expression databases

BgeeiENSG00000137331 Expressed in 227 organ(s), highest expression level in endometrium
CleanExiHS_IER3
ExpressionAtlasiP46695 baseline and differential
GenevisibleiP46695 HS

Organism-specific databases

HPAiHPA043847

Interactioni

Subunit structurei

Interacts with the PPP2R5C-PP2A holoenzyme and ERK kinases; regulates ERK dephosphorylation.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi114390, 11 interactors
IntActiP46695, 17 interactors
MINTiP46695
STRINGi9606.ENSP00000259874

Structurei

3D structure databases

ProteinModelPortaliP46695
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IER3 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IZ8Q Eukaryota
ENOG410Z2E4 LUCA
GeneTreeiENSGT00390000003213
HOGENOMiHOG000113001
HOVERGENiHBG000172
InParanoidiP46695
OMAiFCQILMA
OrthoDBiEOG091G0UO1
PhylomeDBiP46695
TreeFamiTF338252

Family and domain databases

InterProiView protein in InterPro
IPR024829 IEX-1
PANTHERiPTHR16915 PTHR16915, 1 hit
PRINTSiPR02100 GENEIEX1

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P46695-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCHSRSCHPT MTILQAPTPA PSTIPGPRRG SGPEIFTFDP LPEPAAAPAG
60 70 80 90 100
RPSASRGHRK RSRRVLYPRV VRRQLPVEEP NPAKRLLFLL LTIVFCQILM
110 120 130 140 150
AEEGVPAPLP PEDAPNAASL APTPVSAVLE PFNLTSEPSD YALDLSTFLQ

QHPAAF
Length:156
Mass (Da):16,903
Last modified:January 11, 2011 - v4
Checksum:i83C06116C81B8341
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5ST79Q5ST79_HUMAN
Immediate early response 3, isoform...
IER3 hCG_26002
138Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54A → G in AAB36278 (PubMed:8603392).Curated1
Sequence conflicti106P → R in AAB36278 (PubMed:8603392).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_058496127A → P6 PublicationsCorresponds to variant dbSNP:rs3094124Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81914 mRNA Translation: AAB36278.1
X96438 Genomic DNA Translation: CAA65304.1
Y14551 mRNA Translation: CAA74886.1
BT006703 mRNA Translation: AAP35349.1
BA000025 Genomic DNA Translation: BAB63319.1
AL662797 Genomic DNA No translation available.
BC000844 mRNA Translation: AAH00844.1
BC005080 mRNA Translation: AAH05080.1
CCDSiCCDS4689.1
PIRiJC5537
RefSeqiNP_003888.2, NM_003897.3
UniGeneiHs.730861
Hs.76095

Genome annotation databases

EnsembliENST00000259874; ENSP00000259874; ENSG00000137331
ENST00000383560; ENSP00000373054; ENSG00000206478
ENST00000416884; ENSP00000406245; ENSG00000227231
ENST00000435856; ENSP00000412283; ENSG00000235030
ENST00000439190; ENSP00000397956; ENSG00000237155
ENST00000450236; ENSP00000398139; ENSG00000230128
GeneIDi8870
KEGGihsa:8870
UCSCiuc003nrn.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81914 mRNA Translation: AAB36278.1
X96438 Genomic DNA Translation: CAA65304.1
Y14551 mRNA Translation: CAA74886.1
BT006703 mRNA Translation: AAP35349.1
BA000025 Genomic DNA Translation: BAB63319.1
AL662797 Genomic DNA No translation available.
BC000844 mRNA Translation: AAH00844.1
BC005080 mRNA Translation: AAH05080.1
CCDSiCCDS4689.1
PIRiJC5537
RefSeqiNP_003888.2, NM_003897.3
UniGeneiHs.730861
Hs.76095

3D structure databases

ProteinModelPortaliP46695
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114390, 11 interactors
IntActiP46695, 17 interactors
MINTiP46695
STRINGi9606.ENSP00000259874

PTM databases

iPTMnetiP46695
PhosphoSitePlusiP46695

Polymorphism and mutation databases

BioMutaiIER3
DMDMi317373569

Proteomic databases

PaxDbiP46695
PeptideAtlasiP46695
PRIDEiP46695
ProteomicsDBi55747

Protocols and materials databases

DNASUi8870
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000259874; ENSP00000259874; ENSG00000137331
ENST00000383560; ENSP00000373054; ENSG00000206478
ENST00000416884; ENSP00000406245; ENSG00000227231
ENST00000435856; ENSP00000412283; ENSG00000235030
ENST00000439190; ENSP00000397956; ENSG00000237155
ENST00000450236; ENSP00000398139; ENSG00000230128
GeneIDi8870
KEGGihsa:8870
UCSCiuc003nrn.4 human

Organism-specific databases

CTDi8870
DisGeNETi8870
EuPathDBiHostDB:ENSG00000137331.11
GeneCardsiIER3
HGNCiHGNC:5392 IER3
HPAiHPA043847
MIMi602996 gene
neXtProtiNX_P46695
OpenTargetsiENSG00000137331
PharmGKBiPA29639
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IZ8Q Eukaryota
ENOG410Z2E4 LUCA
GeneTreeiENSGT00390000003213
HOGENOMiHOG000113001
HOVERGENiHBG000172
InParanoidiP46695
OMAiFCQILMA
OrthoDBiEOG091G0UO1
PhylomeDBiP46695
TreeFamiTF338252

Enzyme and pathway databases

ReactomeiR-HSA-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
SIGNORiP46695

Miscellaneous databases

ChiTaRSiIER3 human
GeneWikiiIER3
GenomeRNAii8870
PROiPR:P46695
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137331 Expressed in 227 organ(s), highest expression level in endometrium
CleanExiHS_IER3
ExpressionAtlasiP46695 baseline and differential
GenevisibleiP46695 HS

Family and domain databases

InterProiView protein in InterPro
IPR024829 IEX-1
PANTHERiPTHR16915 PTHR16915, 1 hit
PRINTSiPR02100 GENEIEX1
ProtoNetiSearch...

Entry informationi

Entry nameiIEX1_HUMAN
AccessioniPrimary (citable) accession number: P46695
Secondary accession number(s): Q5SU30, Q92691, Q93044
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 11, 2011
Last modified: November 7, 2018
This is version 151 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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