UniProtKB - P46674 (SAC3_YEAST)
Nuclear mRNA export protein SAC3
SAC3
Functioni
Component of the SAC3-THP1 complex, which functions in transcription-coupled mRNA export from the nucleus to the cytoplasm. SAC3-THP1 functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket), by association with components of the nuclear mRNA export machinery (MEX67-MTR2 and SUB2) in the nucleoplasm and the nucleoporin NUP1 at the nuclear basket.
2 PublicationsMiscellaneous
GO - Biological processi
- actin filament-based process Source: SGD
- mitotic cell cycle Source: SGD
- mRNA 3'-end processing Source: SGD
- mRNA export from nucleus Source: SGD
- nuclear mRNA surveillance Source: SGD
- posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
- protein export from nucleus Source: SGD
- ribosomal small subunit biogenesis Source: SGD
- transcription-coupled nucleotide-excision repair Source: SGD
Keywordsi
Biological process | mRNA transport, Transcription, Transcription regulation, Transport |
Protein family/group databases
TCDBi | 3.A.22.1.1, the transcription-coupled trex/tap nuclear mrna export complex (trex) family |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear mRNA export protein SAC3Alternative name(s): Leucine permease transcriptional regulator |
Gene namesi | Name:SAC3 Synonyms:LEP1 Ordered Locus Names:YDR159W ORF Names:YD8358.13 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002566, SAC3 |
VEuPathDBi | FungiDB:YDR159W |
Subcellular locationi
Nucleus
- Nucleus envelope 2 Publications
Note: Localizes to the nuclear pores.
Nucleus
- nuclear pore cytoplasmic filaments Source: SGD
- nucleus Source: GO_Central
- transcription export complex 2 Source: SGD
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097562 | 1 – 1301 | Nuclear mRNA export protein SAC3Add BLAST | 1301 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 568 | PhosphoserineCombined sources | 1 | |
Modified residuei | 748 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 866 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P46674 |
PaxDbi | P46674 |
PRIDEi | P46674 |
PTM databases
iPTMneti | P46674 |
Interactioni
Subunit structurei
Heterodimer with THP1. The SAC3-THP1 complex interacts with CDC31 and SUS1, and with the mRNA export factor MEX67-MTR2, the TREX complex component SUB2, and the nucleoporin NUP1. SAC3 directly interacts with MEX67, NUP1 and SUB2.
3 PublicationsBinary interactionsi
P46674
With | #Exp. | IntAct |
---|---|---|
CDC31 [P06704] | 10 | EBI-16425,EBI-4259 |
SUS1 [Q6WNK7] | 11 | EBI-16425,EBI-1251050 |
Protein-protein interaction databases
BioGRIDi | 32210, 918 interactors |
ComplexPortali | CPX-1686, TREX-2 transcription-export complex CPX-1687, Thp1-Sac3 complex |
DIPi | DIP-2444N |
IntActi | P46674, 14 interactors |
MINTi | P46674 |
STRINGi | 4932.YDR159W |
Miscellaneous databases
RNActi | P46674, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P46674 |
SMRi | P46674 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P46674 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 333 – 524 | PCIPROSITE-ProRule annotationAdd BLAST | 192 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 572 | Required for interaction with MEX67, SUB2 and THP11 PublicationAdd BLAST | 572 | |
Regioni | 54 – 82 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 573 – 1301 | Required for targeting SAC3 to the nuclear poreAdd BLAST | 729 | |
Regioni | 689 – 715 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 733 – 860 | Interaction with CDC311 PublicationAdd BLAST | 128 | |
Regioni | 1164 – 1185 | DisorderedSequence analysisAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 54 – 74 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 691 – 715 | Basic and acidic residuesSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1860, Eukaryota |
GeneTreei | ENSGT00940000160988 |
HOGENOMi | CLU_006094_0_0_1 |
InParanoidi | P46674 |
OMAi | FQGTCLD |
Family and domain databases
DisProti | DP02481 |
IDEALi | IID50280 |
InterProi | View protein in InterPro IPR000717, PCI_dom IPR017173, Sac3 IPR045107, SAC3/GANP/THP3 IPR005062, SAC3/GANP/THP3_conserved IPR024293, SAC3_helical |
PANTHERi | PTHR12436, PTHR12436, 1 hit |
Pfami | View protein in Pfam PF12209, SAC3, 1 hit PF03399, SAC3_GANP, 1 hit |
PIRSFi | PIRSF037320, mRNA_export_factor_Sac3, 1 hit |
PROSITEi | View protein in PROSITE PS50250, PCI, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MNTSFGSVVP STNFNFFKGH GNNDNTSANS TVNNSNFFLN SNETKPSKNV
60 70 80 90 100
FMVHSTSQKK SQQPLQNLSH SPSYTENKPD KKKKYMINDA KTIQLVGPLI
110 120 130 140 150
SSPDNLGFQK RSHKARELPR FLINQEPQLE KRAFVQDPWD KANQEKMISL
160 170 180 190 200
EESIDDLNEL YETLKKMRNT ERSIMEEKGL VDKADSAKDL YDAIVFQGTC
210 220 230 240 250
LDMCPTFERS RRNVEYTVYS YEKNQPNDKK ASRTKALKVF ARPAAAAAPP
260 270 280 290 300
LPSDVRPPHI LVKTLDYIVD NLLTTLPESE GFLWDRMRSI RQDFTYQNYS
310 320 330 340 350
GPEAVDCNER IVRIHLLILH IMVKSNVEFS LQQELEQLHK SLITLSEIYD
360 370 380 390 400
DVRSSGGTCP NEAEFRAYAL LSKIRDPQYD ENIQRLPKHI FQDKLVQMAL
410 420 430 440 450
CFRRVISNSA YTERGFVKTE NCLNFYARFF QLMQSPSLPL LMGFFLQMHL
460 470 480 490 500
TDIRFYALRA LSHTLNKKHK PIPFIYLENM LLFNNRQEII EFCNYYSIEI
510 520 530 540 550
INGDAADLKT LQHYSHKLSE TQPLKKTYLT CLERRLQKTT YKGLINGGED
560 570 580 590 600
NLASSVYVKD PKKDRIPSIA DQSFLMENFQ NNYNEKLNQN SSVKPQINTS
610 620 630 640 650
PKRVATRPNH FPFSQESKQL PQISQSHTLS TNPLLTPQVH GDLSEQKQQQ
660 670 680 690 700
IKTVTDGGSP FVFDQSAQNS TVEASKAHMI STTSNGAYDE KLSSEQEEMR
710 720 730 740 750
KKEEQRIEEE KTQLKKKQEN ADKQVITEQI ANDLVKEVVN SSVISIVKRE
760 770 780 790 800
FSEANYRKDF IDTMTRELYD AFLHERLYLI YMDSRAELKR NSTLKKKFFE
810 820 830 840 850
KWQASYSQAK KNRILEEKKR EEIKLVSHQL GVPGFKKSTC LFRTPYKGNV
860 870 880 890 900
NSSFMLSSSD KNLIFSPVND EFNKFATHLT KISKLWRPLE MQSIYYDNLT
910 920 930 940 950
KKFPSNSLTP ANLFIYAKDW TSLSNRWILS KFNLQTAQDS KKFSNNIISS
960 970 980 990 1000
RIICIDDEYE PSDFSDLQLL IFNTGVTNPD IFDLEMKLKD DGEELIKLIT
1010 1020 1030 1040 1050
GISLNTNICF SLLIIYWESA ENTLSESTIK HLLKLNRISK NYSSVIERID
1060 1070 1080 1090 1100
LMNLTEESPH KCLEDKLSEI SHSYVYKLTE RGKYDKTLRQ KRSLAGIHSR
1110 1120 1130 1140 1150
STQLQTTKDI DQKMKKMLEK EKNKYQQQIG ERNTYAHLES HIDASPRSKK
1160 1170 1180 1190 1200
RKLPILLSTS HSSQFKTPLA SRLNTSGSST SPPLPSHLAM KFRKNSRVTS
1210 1220 1230 1240 1250
LHTVLPVSTP SHSNNIPAAS FSGNNTTDIQ SQQLIENQKS TSVYLNNVSE
1260 1270 1280 1290 1300
RILGNQEICQ TPINPVTPVL DGADQGKEDI PDSILELKIL IDSVKKKVNN
D
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z47805 Genomic DNA Translation: CAA87767.1 Z50046 Genomic DNA Translation: CAA90379.1 U35227 Genomic DNA Translation: AAA79056.1 BK006938 Genomic DNA Translation: DAA11999.1 |
PIRi | S51323 |
RefSeqi | NP_010443.3, NM_001180466.3 |
Genome annotation databases
EnsemblFungii | YDR159W_mRNA; YDR159W; YDR159W |
GeneIDi | 851737 |
KEGGi | sce:YDR159W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z47805 Genomic DNA Translation: CAA87767.1 Z50046 Genomic DNA Translation: CAA90379.1 U35227 Genomic DNA Translation: AAA79056.1 BK006938 Genomic DNA Translation: DAA11999.1 |
PIRi | S51323 |
RefSeqi | NP_010443.3, NM_001180466.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FWB | X-ray | 2.50 | B | 752-805 | [»] | |
3FWC | X-ray | 2.70 | B/F/J/N | 723-805 | [»] | |
3T5V | X-ray | 2.90 | A/D | 250-563 | [»] | |
4C31 | X-ray | 3.00 | A/D | 757-787 | [»] | |
4MBE | X-ray | 2.61 | B/E | 753-805 | [»] | |
4TRQ | X-ray | 3.10 | A/D | 253-551 | [»] | |
5G5P | electron microscopy | 5.30 | A | 1-805 | [»] | |
5L3T | X-ray | 4.93 | A | 1-1301 | [»] | |
AlphaFoldDBi | P46674 | |||||
SMRi | P46674 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32210, 918 interactors |
ComplexPortali | CPX-1686, TREX-2 transcription-export complex CPX-1687, Thp1-Sac3 complex |
DIPi | DIP-2444N |
IntActi | P46674, 14 interactors |
MINTi | P46674 |
STRINGi | 4932.YDR159W |
Protein family/group databases
TCDBi | 3.A.22.1.1, the transcription-coupled trex/tap nuclear mrna export complex (trex) family |
PTM databases
iPTMneti | P46674 |
Proteomic databases
MaxQBi | P46674 |
PaxDbi | P46674 |
PRIDEi | P46674 |
Genome annotation databases
EnsemblFungii | YDR159W_mRNA; YDR159W; YDR159W |
GeneIDi | 851737 |
KEGGi | sce:YDR159W |
Organism-specific databases
SGDi | S000002566, SAC3 |
VEuPathDBi | FungiDB:YDR159W |
Phylogenomic databases
eggNOGi | KOG1860, Eukaryota |
GeneTreei | ENSGT00940000160988 |
HOGENOMi | CLU_006094_0_0_1 |
InParanoidi | P46674 |
OMAi | FQGTCLD |
Miscellaneous databases
EvolutionaryTracei | P46674 |
PROi | PR:P46674 |
RNActi | P46674, protein |
Family and domain databases
DisProti | DP02481 |
IDEALi | IID50280 |
InterProi | View protein in InterPro IPR000717, PCI_dom IPR017173, Sac3 IPR045107, SAC3/GANP/THP3 IPR005062, SAC3/GANP/THP3_conserved IPR024293, SAC3_helical |
PANTHERi | PTHR12436, PTHR12436, 1 hit |
Pfami | View protein in Pfam PF12209, SAC3, 1 hit PF03399, SAC3_GANP, 1 hit |
PIRSFi | PIRSF037320, mRNA_export_factor_Sac3, 1 hit |
PROSITEi | View protein in PROSITE PS50250, PCI, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SAC3_YEAST | |
Accessioni | P46674Primary (citable) accession number: P46674 Secondary accession number(s): D6VSD9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families