UniProtKB - P46662 (MERL_MOUSE)
Merlin
Nf2
Functioni
GO - Molecular functioni
- actin binding Source: InterPro
- beta-catenin binding Source: MGI
- integrin binding Source: MGI
- protein domain specific binding Source: MGI
GO - Biological processi
- actin cytoskeleton organization Source: MGI
- brain development Source: MGI
- cell-cell junction organization Source: MGI
- ectoderm development Source: MGI
- hippocampus development Source: MGI
- lens fiber cell differentiation Source: UniProtKB
- mesoderm formation Source: MGI
- negative regulation of cell-cell adhesion Source: MGI
- negative regulation of cell growth Source: MGI
- negative regulation of cell population proliferation Source: MGI
- negative regulation of MAPK cascade Source: MGI
- negative regulation of protein kinase activity Source: MGI
- negative regulation of receptor signaling pathway via JAK-STAT Source: MGI
- negative regulation of tyrosine phosphorylation of STAT protein Source: MGI
- odontogenesis of dentin-containing tooth Source: MGI
- positive regulation of cell differentiation Source: MGI
- positive regulation of stress fiber assembly Source: MGI
- regulation of apoptotic process Source: UniProtKB
- regulation of cell cycle Source: UniProtKB
- regulation of cell population proliferation Source: MGI
- regulation of gliogenesis Source: MGI
- regulation of hippo signaling Source: MGI
- regulation of neural precursor cell proliferation Source: MGI
- regulation of neurogenesis Source: MGI
- regulation of protein localization to nucleus Source: MGI
- regulation of protein stability Source: MGI
- regulation of stem cell proliferation Source: MGI
- Schwann cell proliferation Source: MGI
Enzyme and pathway databases
Reactomei | R-MMU-2029482, Regulation of actin dynamics for phagocytic cup formation R-MMU-5627123, RHO GTPases activate PAKs |
Names & Taxonomyi
Protein namesi | Recommended name: MerlinAlternative name(s): Moesin-ezrin-radixin-like protein Neurofibromin-2 Schwannomin |
Gene namesi | Name:Nf2 Synonyms:Nf-2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97307, Nf2 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Nucleus
- Nucleus By similarity
Other locations
- Cell projection By similarity
Note: Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with DCAF1 in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions (By similarity).By similarity
Cytoskeleton
- cortical actin cytoskeleton Source: MGI
- cytoskeleton Source: MGI
Cytosol
- cytosol Source: MGI
Endosome
- early endosome Source: MGI
Nucleus
Plasma Membrane
- cleavage furrow Source: MGI
- plasma membrane Source: MGI
Other locations
- adherens junction Source: MGI
- apical part of cell Source: MGI
- cell body Source: MGI
- cytoplasm Source: MGI
- filopodium Source: MGI
- lamellipodium Source: MGI
- membrane raft Source: MGI
- neuron projection Source: MGI
- perinuclear region of cytoplasm Source: MGI
- protein-containing complex Source: MGI
- ruffle Source: MGI
- synapse Source: MGI
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
Keywords - Diseasei
Tumor suppressorPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219413 | 1 – 596 | MerlinAdd BLAST | 596 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 13 | PhosphoserineBy similarity | 1 | |
Modified residuei | 518 | Phosphoserine; by PAKBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P46662 |
jPOSTi | P46662 |
PaxDbi | P46662 |
PRIDEi | P46662 |
PTM databases
iPTMneti | P46662 |
PhosphoSitePlusi | P46662 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000009073, Expressed in submandibular gland and 328 other tissues |
ExpressionAtlasi | P46662, baseline and differential |
Genevisiblei | P46662, MM |
Interactioni
Subunit structurei
Interacts with SLC9A3R1, HGS and AGAP2.
Interacts with SGSM3.
Interacts (via FERM domain) with MPP1 (By similarity).
Interacts with LAYN (PubMed:15913605).
Interacts with WWC1.
Interacts with the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. The unphosphorylated form interacts (via FERM domain) with VPRBP/DCAF1.
Interacts (via FERM domain) with NOP53; the interaction is direct (By similarity).
Interacts with SCHIP1; the interaction is direct (By similarity).
By similarity1 PublicationBinary interactionsi
Hide detailsP46662
With | #Exp. | IntAct |
---|---|---|
AMOT [Q4VCS5] from Homo sapiens. | 2 | EBI-644586,EBI-2511319 |
LATS1 [O95835] from Homo sapiens. | 5 | EBI-644586,EBI-444209 |
GO - Molecular functioni
- actin binding Source: InterPro
- beta-catenin binding Source: MGI
- integrin binding Source: MGI
- protein domain specific binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201737, 14 interactors |
IntActi | P46662, 9 interactors |
STRINGi | 10090.ENSMUSP00000105536 |
Miscellaneous databases
RNActi | P46662, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P46662 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P46662 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 311 | FERMPROSITE-ProRule annotationAdd BLAST | 290 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 327 – 465 | Glu-richAdd BLAST | 139 |
Phylogenomic databases
eggNOGi | KOG3529, Eukaryota |
GeneTreei | ENSGT01010000222397 |
HOGENOMi | CLU_003623_6_1_1 |
InParanoidi | P46662 |
OMAi | NRITPRP |
OrthoDBi | 627741at2759 |
PhylomeDBi | P46662 |
TreeFami | TF313935 |
Family and domain databases
CDDi | cd14473, FERM_B-lobe, 1 hit cd13194, FERM_C_ERM, 1 hit |
Gene3Di | 1.20.80.10, 1 hit 1.25.40.1020, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR019749, Band_41_domain IPR011174, ERM IPR011259, ERM_C_dom IPR041789, ERM_FERM_C IPR000798, Ez/rad/moesin-like IPR014352, FERM/acyl-CoA-bd_prot_sf IPR035963, FERM_2 IPR019748, FERM_central IPR019747, FERM_CS IPR000299, FERM_domain IPR018979, FERM_N IPR018980, FERM_PH-like_C IPR008954, Moesin_tail_sf IPR011993, PH-like_dom_sf IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF00769, ERM, 1 hit PF09380, FERM_C, 1 hit PF00373, FERM_M, 1 hit PF09379, FERM_N, 1 hit |
PIRSFi | PIRSF002305, ERM, 1 hit |
PRINTSi | PR00935, BAND41 PR00661, ERMFAMILY |
SMARTi | View protein in SMART SM00295, B41, 1 hit SM01196, FERM_C, 1 hit |
SUPFAMi | SSF47031, SSF47031, 1 hit SSF48678, SSF48678, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00660, FERM_1, 1 hit PS00661, FERM_2, 1 hit PS50057, FERM_3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGAIASRMS FSSLKRKQPK TFTVRIVTMD AEMEFNCEMK WKGKDLFDLV
60 70 80 90 100
CRTLGLRETW FFGLQYTIKD TVAWLKMDKK VLDHDVSKEE PVTFHFLAKF
110 120 130 140 150
YPENAEEELV QEITQHLFFL QVKKQILDEK VYCPPEASVL LASYAVQAKY
160 170 180 190 200
GDYDPSVHKR GFLAQEELLP KRVINLYQMT PEMWEERITA WYAEHRGRAR
210 220 230 240 250
DEAEMEYLKI AQDLEMYGVN YFTIRNKKGT ELLLGVDALG LHIYDPENRL
260 270 280 290 300
TPKISFPWNE IRNISYSDKE FTIKPLDKKI DVFKFNSSKL RVNKLILQLC
310 320 330 340 350
IGNHDLFMRR RKADSLEVQQ MKAQAREEKA RKQMERQRLA REKQMREEAE
360 370 380 390 400
RTRDELERRL LQMKEEATMA NEALMRSEET ADLLAEKAQI TEEEAKLLAQ
410 420 430 440 450
KAAEAEQEMQ RIKATAIRTE EEKRLMEQKV LEAEVLALKM AEESERRAKE
460 470 480 490 500
ADQLKQDLQE AREAERRAKQ KLLEIATKPT YPPMNPIPPP LPPDIPSFDI
510 520 530 540 550
IADSLSFDFK DTDMKRLSME IEKEKVEYME KSKHLQEQLN ELKTEIEALK
560 570 580 590
LKERETALDV LHSESSDRGG PSSKHNTIKK LTLQSAKSRV AFFEEL
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8WJ43 | F8WJ43_MOUSE | Merlin | Nf2 | 521 | Annotation score: | ||
F7DBY2 | F7DBY2_MOUSE | Merlin | Nf2 | 140 | Annotation score: | ||
E9PW99 | E9PW99_MOUSE | Merlin | Nf2 | 251 | Annotation score: | ||
F7D6P2 | F7D6P2_MOUSE | Merlin | Nf2 | 159 | Annotation score: | ||
F6X089 | F6X089_MOUSE | Merlin | Nf2 | 83 | Annotation score: | ||
E9Q7I0 | E9Q7I0_MOUSE | Merlin | Nf2 | 131 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 475 | I → T in AAA39808 (PubMed:7981675).Curated | 1 | |
Sequence conflicti | 554 | R → A in AAA39807 (PubMed:8012352).Curated | 1 | |
Sequence conflicti | 554 | R → A in AAA63648 (PubMed:8012352).Curated | 1 | |
Sequence conflicti | 570 | G → A in AAA39808 (PubMed:7981675).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000493 | 581 – 596 | LTLQS…FFEEL → PQAQGRRPICI in isoform 2. 1 PublicationAdd BLAST | 16 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74671 mRNA Translation: CAA52737.1 L27105 mRNA Translation: AAA39807.1 L27090 mRNA Translation: AAA63648.1 L28176 mRNA Translation: AAA39808.1 AK045998 mRNA Translation: BAC32567.1 X75759 mRNA Translation: CAA53386.1 |
CCDSi | CCDS24391.1 [P46662-1] CCDS56757.1 [P46662-2] |
PIRi | I48683 I54368 I68664 |
RefSeqi | NP_001239179.1, NM_001252250.1 [P46662-2] NP_001239180.1, NM_001252251.1 [P46662-2] NP_001239181.1, NM_001252252.1 NP_001239182.1, NM_001252253.1 NP_035028.2, NM_010898.4 [P46662-1] XP_006514633.1, XM_006514570.2 [P46662-2] XP_011241971.1, XM_011243669.2 [P46662-2] |
Genome annotation databases
Ensembli | ENSMUST00000053079; ENSMUSP00000055033; ENSMUSG00000009073 [P46662-2] ENSMUST00000056290; ENSMUSP00000055061; ENSMUSG00000009073 [P46662-2] ENSMUST00000109910; ENSMUSP00000105536; ENSMUSG00000009073 [P46662-1] |
GeneIDi | 18016 |
KEGGi | mmu:18016 |
UCSCi | uc007hvf.2, mouse [P46662-1] uc007hvg.2, mouse [P46662-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74671 mRNA Translation: CAA52737.1 L27105 mRNA Translation: AAA39807.1 L27090 mRNA Translation: AAA63648.1 L28176 mRNA Translation: AAA39808.1 AK045998 mRNA Translation: BAC32567.1 X75759 mRNA Translation: CAA53386.1 |
CCDSi | CCDS24391.1 [P46662-1] CCDS56757.1 [P46662-2] |
PIRi | I48683 I54368 I68664 |
RefSeqi | NP_001239179.1, NM_001252250.1 [P46662-2] NP_001239180.1, NM_001252251.1 [P46662-2] NP_001239181.1, NM_001252252.1 NP_001239182.1, NM_001252253.1 NP_035028.2, NM_010898.4 [P46662-1] XP_006514633.1, XM_006514570.2 [P46662-2] XP_011241971.1, XM_011243669.2 [P46662-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ISN | X-ray | 2.90 | A | 18-340 | [»] | |
3WA0 | X-ray | 2.31 | A/B/C/D/E/F | 19-314 | [»] | |
4P7I | X-ray | 2.60 | A/B | 1-313 | [»] | |
4ZRK | X-ray | 2.32 | A/B/C/D | 1-320 | [»] | |
SMRi | P46662 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 201737, 14 interactors |
IntActi | P46662, 9 interactors |
STRINGi | 10090.ENSMUSP00000105536 |
PTM databases
iPTMneti | P46662 |
PhosphoSitePlusi | P46662 |
Proteomic databases
EPDi | P46662 |
jPOSTi | P46662 |
PaxDbi | P46662 |
PRIDEi | P46662 |
Protocols and materials databases
Antibodypediai | 319, 636 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000053079; ENSMUSP00000055033; ENSMUSG00000009073 [P46662-2] ENSMUST00000056290; ENSMUSP00000055061; ENSMUSG00000009073 [P46662-2] ENSMUST00000109910; ENSMUSP00000105536; ENSMUSG00000009073 [P46662-1] |
GeneIDi | 18016 |
KEGGi | mmu:18016 |
UCSCi | uc007hvf.2, mouse [P46662-1] uc007hvg.2, mouse [P46662-2] |
Organism-specific databases
CTDi | 4771 |
MGIi | MGI:97307, Nf2 |
Phylogenomic databases
eggNOGi | KOG3529, Eukaryota |
GeneTreei | ENSGT01010000222397 |
HOGENOMi | CLU_003623_6_1_1 |
InParanoidi | P46662 |
OMAi | NRITPRP |
OrthoDBi | 627741at2759 |
PhylomeDBi | P46662 |
TreeFami | TF313935 |
Enzyme and pathway databases
Reactomei | R-MMU-2029482, Regulation of actin dynamics for phagocytic cup formation R-MMU-5627123, RHO GTPases activate PAKs |
Miscellaneous databases
BioGRID-ORCSi | 18016, 7 hits in 22 CRISPR screens |
ChiTaRSi | Nf2, mouse |
EvolutionaryTracei | P46662 |
PROi | PR:P46662 |
RNActi | P46662, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000009073, Expressed in submandibular gland and 328 other tissues |
ExpressionAtlasi | P46662, baseline and differential |
Genevisiblei | P46662, MM |
Family and domain databases
CDDi | cd14473, FERM_B-lobe, 1 hit cd13194, FERM_C_ERM, 1 hit |
Gene3Di | 1.20.80.10, 1 hit 1.25.40.1020, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR019749, Band_41_domain IPR011174, ERM IPR011259, ERM_C_dom IPR041789, ERM_FERM_C IPR000798, Ez/rad/moesin-like IPR014352, FERM/acyl-CoA-bd_prot_sf IPR035963, FERM_2 IPR019748, FERM_central IPR019747, FERM_CS IPR000299, FERM_domain IPR018979, FERM_N IPR018980, FERM_PH-like_C IPR008954, Moesin_tail_sf IPR011993, PH-like_dom_sf IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF00769, ERM, 1 hit PF09380, FERM_C, 1 hit PF00373, FERM_M, 1 hit PF09379, FERM_N, 1 hit |
PIRSFi | PIRSF002305, ERM, 1 hit |
PRINTSi | PR00935, BAND41 PR00661, ERMFAMILY |
SMARTi | View protein in SMART SM00295, B41, 1 hit SM01196, FERM_C, 1 hit |
SUPFAMi | SSF47031, SSF47031, 1 hit SSF48678, SSF48678, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00660, FERM_1, 1 hit PS00661, FERM_2, 1 hit PS50057, FERM_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MERL_MOUSE | |
Accessioni | P46662Primary (citable) accession number: P46662 Secondary accession number(s): Q8BR03 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | June 27, 2003 | |
Last modified: | December 2, 2020 | |
This is version 182 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references