UniProtKB - P46660 (AINX_MOUSE)
Protein
Alpha-internexin
Gene
Ina
Organism
Mus musculus (Mouse)
Status
Functioni
Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges (By similarity). May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks (PubMed:22723690).By similarity1 Publication
GO - Molecular functioni
- protein-containing complex binding Source: MGI
- structural constituent of postsynaptic actin cytoskeleton Source: SynGO
- structural constituent of postsynaptic intermediate filament cytoskeleton Source: SynGO
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- cellular response to leukemia inhibitory factor Source: MGI
- intermediate filament cytoskeleton organization Source: MGI
- nervous system development Source: UniProtKB-KW
- neurofilament cytoskeleton organization Source: MGI
Keywordsi
Molecular function | Developmental protein |
Biological process | Differentiation, Neurogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-internexinShort name: Alpha-Inx Alternative name(s): 66 kDa neurofilament protein Short name: NF-66 Short name: Neurofilament-66 |
Gene namesi | Name:Ina |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96568, Ina |
Subcellular locationi
Cytoskeleton
- intermediate filament cytoskeleton Source: MGI
- neurofilament Source: MGI
- postsynaptic intermediate filament cytoskeleton Source: MGI
Other locations
- cytoplasmic ribonucleoprotein granule Source: MGI
- glutamatergic synapse Source: MGI
- myelin sheath Source: UniProtKB
- postsynapse Source: SynGO
- Schaffer collateral - CA1 synapse Source: SynGO
Keywords - Cellular componenti
Intermediate filamentPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063784 | 1 – 501 | Alpha-internexinAdd BLAST | 501 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 72 | PhosphoserineCombined sources | 1 | |
Modified residuei | 219 | PhosphoserineCombined sources | 1 | |
Modified residuei | 290 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 335 | PhosphoserineCombined sources | 1 | |
Modified residuei | 498 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
O-glycosylated.1 Publication
Keywords - PTMi
Acetylation, Glycoprotein, PhosphoproteinProteomic databases
EPDi | P46660 |
jPOSTi | P46660 |
MaxQBi | P46660 |
PaxDbi | P46660 |
PeptideAtlasi | P46660 |
PRIDEi | P46660 |
2D gel databases
UCD-2DPAGEi | P46660 |
PTM databases
iPTMneti | P46660 |
PhosphoSitePlusi | P46660 |
SwissPalmi | P46660 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000034336, Expressed in dorsal root ganglion and 193 other tissues |
Genevisiblei | P46660, MM |
Interactioni
Subunit structurei
Forms homodimers (in vitro) (By similarity). Forms heterodimers with NEFL, NEFM or NEFH (in vitro) (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 230487, 39 interactors |
IntActi | P46660, 20 interactors |
MINTi | P46660 |
STRINGi | 10090.ENSMUSP00000041347 |
Miscellaneous databases
RNActi | P46660, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 94 – 407 | IF rodPROSITE-ProRule annotationAdd BLAST | 314 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 87 | HeadAdd BLAST | 87 | |
Regioni | 88 – 129 | Coil 1AAdd BLAST | 42 | |
Regioni | 130 – 142 | Linker 1Add BLAST | 13 | |
Regioni | 143 – 238 | Coil 1BAdd BLAST | 96 | |
Regioni | 239 – 262 | Linker 2Add BLAST | 24 | |
Regioni | 263 – 408 | Coil 2Add BLAST | 146 | |
Regioni | 409 – 501 | TailAdd BLAST | 93 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 449 – 456 | Poly-Glu | 8 |
Sequence similaritiesi
Belongs to the intermediate filament family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502RAU0, Eukaryota |
GeneTreei | ENSGT00940000154418 |
HOGENOMi | CLU_012560_7_3_1 |
InParanoidi | P46660 |
OMAi | EHYLCSA |
OrthoDBi | 888678at2759 |
TreeFami | TF330122 |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit |
InterProi | View protein in InterPro IPR027703, Alpha-Inx IPR018039, IF_conserved IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR006821, Intermed_filament_DNA-bd |
PANTHERi | PTHR45652:SF18, PTHR45652:SF18, 1 hit |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF04732, Filament_head, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P46660-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSFGSEHYLC SASSYRKVFG DSSRLSARLS GPGGSGSFRS QSLSRSNVAS
60 70 80 90 100
TAACSSASSL GLGLAYRRLP ASDGLDLSQA AARTNEYKII RTNEKEQLQG
110 120 130 140 150
LNDRFAVFIE KVHQLETQNR ALEAELAALR QRHAEPSRVG ELFQRELREL
160 170 180 190 200
RAQLEEASSA RAQALLERDG LAEEVQRLRA RCEEESRGRE GAERALKAQQ
210 220 230 240 250
RDVDGATLAR LDLEKKVESL LDELAFVRQV HDEEVAELLA TLQASSQAAA
260 270 280 290 300
EVDVAVAKPD LTSALREIRA QYESLAAKNL QSAEEWYKSK FANLNEQAAR
310 320 330 340 350
STEAIRASRE EIHEYRRQLQ ARTIEIEGLR GANESLERQI LELEERHSAE
360 370 380 390 400
VAGYQDSIGQ LESDLRNTKS EMARHLREYQ DLLNVKMALD IEIAAYRKLL
410 420 430 440 450
EGEETRFSTG GLSISGLNPL PNPSYLLPPR ILSSTASKVS SAGLSLKKEE
460 470 480 490 500
EEEEEEASKE VSKKTSKVGE GFEETLGEAV ISTKKTGKSA TEESTSSSQK
M
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 28 | R → P in AAB37740 (PubMed:8734438).Curated | 1 | |
Sequence conflicti | 34 | G → A in AAB37740 (PubMed:8734438).Curated | 1 | |
Sequence conflicti | 141 | E → Q in AAB37740 (PubMed:8734438).Curated | 1 | |
Sequence conflicti | 147 | L → R in AAB37740 (PubMed:8734438).Curated | 1 | |
Sequence conflicti | 163 | Q → E in AAA62617 (PubMed:7959004).Curated | 1 | |
Sequence conflicti | 297 | Q → L in AAB37740 (PubMed:8734438).Curated | 1 | |
Sequence conflicti | 301 | S → T in AAB37740 (PubMed:8734438).Curated | 1 | |
Sequence conflicti | 318 – 319 | QL → HV in AAB37740 (PubMed:8734438).Curated | 2 | |
Sequence conflicti | 347 | H → D in AAA62617 (PubMed:7959004).Curated | 1 | |
Sequence conflicti | 383 | L → F in AAA62617 (PubMed:7959004).Curated | 1 | |
Sequence conflicti | 388 | A → G in AAA62617 (PubMed:7959004).Curated | 1 | |
Sequence conflicti | 403 | E → N in AAB37740 (PubMed:8734438).Curated | 1 | |
Sequence conflicti | 442 | A → T in AAA62617 (PubMed:7959004).Curated | 1 | |
Sequence conflicti | 442 | A → T in AAB37740 (PubMed:8734438).Curated | 1 | |
Sequence conflicti | 443 | G → R in AAA62617 (PubMed:7959004).Curated | 1 | |
Sequence conflicti | 450 | E → D in AAA62617 (PubMed:7959004).Curated | 1 | |
Sequence conflicti | 450 | E → D in AAB37740 (PubMed:8734438).Curated | 1 | |
Sequence conflicti | 455 | E → EEEE in AAA62617 (PubMed:7959004).Curated | 1 | |
Sequence conflicti | 456 | E → D in AAA62617 (PubMed:7959004).Curated | 1 | |
Sequence conflicti | 456 | E → D in AAB37740 (PubMed:8734438).Curated | 1 | |
Sequence conflicti | 489 | S → L in AAB37740 (PubMed:8734438).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L27220 Genomic DNA Translation: AAA62617.1 L36390 mRNA Translation: AAB37740.1 BC018383 mRNA Translation: AAH18383.1 |
CCDSi | CCDS38013.1 |
PIRi | I53868 |
RefSeqi | NP_666212.3, NM_146100.4 |
Genome annotation databases
Ensembli | ENSMUST00000037636; ENSMUSP00000041347; ENSMUSG00000034336 |
GeneIDi | 226180 |
KEGGi | mmu:226180 |
UCSCi | uc008huk.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L27220 Genomic DNA Translation: AAA62617.1 L36390 mRNA Translation: AAB37740.1 BC018383 mRNA Translation: AAH18383.1 |
CCDSi | CCDS38013.1 |
PIRi | I53868 |
RefSeqi | NP_666212.3, NM_146100.4 |
3D structure databases
SMRi | P46660 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230487, 39 interactors |
IntActi | P46660, 20 interactors |
MINTi | P46660 |
STRINGi | 10090.ENSMUSP00000041347 |
PTM databases
iPTMneti | P46660 |
PhosphoSitePlusi | P46660 |
SwissPalmi | P46660 |
2D gel databases
UCD-2DPAGEi | P46660 |
Proteomic databases
EPDi | P46660 |
jPOSTi | P46660 |
MaxQBi | P46660 |
PaxDbi | P46660 |
PeptideAtlasi | P46660 |
PRIDEi | P46660 |
Protocols and materials databases
Antibodypediai | 1538, 473 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000037636; ENSMUSP00000041347; ENSMUSG00000034336 |
GeneIDi | 226180 |
KEGGi | mmu:226180 |
UCSCi | uc008huk.1, mouse |
Organism-specific databases
CTDi | 9118 |
MGIi | MGI:96568, Ina |
Phylogenomic databases
eggNOGi | ENOG502RAU0, Eukaryota |
GeneTreei | ENSGT00940000154418 |
HOGENOMi | CLU_012560_7_3_1 |
InParanoidi | P46660 |
OMAi | EHYLCSA |
OrthoDBi | 888678at2759 |
TreeFami | TF330122 |
Miscellaneous databases
BioGRID-ORCSi | 226180, 4 hits in 17 CRISPR screens |
ChiTaRSi | Ina, mouse |
PROi | PR:P46660 |
RNActi | P46660, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034336, Expressed in dorsal root ganglion and 193 other tissues |
Genevisiblei | P46660, MM |
Family and domain databases
Gene3Di | 1.20.5.1160, 1 hit |
InterProi | View protein in InterPro IPR027703, Alpha-Inx IPR018039, IF_conserved IPR039008, IF_rod_dom IPR042180, IF_rod_dom_coil1B IPR006821, Intermed_filament_DNA-bd |
PANTHERi | PTHR45652:SF18, PTHR45652:SF18, 1 hit |
Pfami | View protein in Pfam PF00038, Filament, 1 hit PF04732, Filament_head, 1 hit |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AINX_MOUSE | |
Accessioni | P46660Primary (citable) accession number: P46660 Secondary accession number(s): Q61958, Q8VCW5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | January 22, 2014 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families