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Protein

Glutamate--tRNA ligase, cytoplasmic

Gene

GUS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). In mitochondria, constitutes the nondiscriminating glutamyl-tRNA synthase that generates the mitochondrial mischarged glutamyl-tRNA(Gln) substrate for the tRNA-dependent amidotransferase (AdT), which generates mitochondrial glutaminyl-tRNA(Gln) by transamidation of glutamyl-tRNA(Gln).2 Publications

Miscellaneous

Present with 48700 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei215ATPBy similarity1
Binding sitei241GlutamateBy similarity1
Binding sitei400GlutamateBy similarity1
Binding sitei403ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi437 – 441ATPBy similarity5

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate-tRNA ligase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30716-MONOMER
BRENDAi6.1.1.17 984
SABIO-RKiP46655

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase, cytoplasmic (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
(c)ERS
Short name:
GluRS
P85
Gene namesi
Name:GUS1
Ordered Locus Names:YGL245W
ORF Names:G0583, HRB724
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL245W
SGDiS000003214 GUS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi148R → A: Abolishes interaction with ARC1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197391 – 708Glutamate--tRNA ligase, cytoplasmicAdd BLAST708

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei300PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46655
PaxDbiP46655
PRIDEiP46655

PTM databases

iPTMnetiP46655

Interactioni

Subunit structurei

Component of a yeast aminoacyl-tRNA synthase (aaRS) complex formed by methionyl-tRNA synthase MES1, glutamyl-tRNA synthase GUS1 and the tRNA aminoacylation cofactor ARC1 in a stoichiometric complex. Interacts (via N-ter) with ARC1 (via N-ter). Can also form a stable binary complex with ARC1 that is functional in terms of aminoacylation. ARC1 increases the affinity for cognate tRNAs due to the presence of a tRNA binding domain in the middle and C-terminal part of ARC1.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARC1P466727EBI-18665,EBI-7224

Protein-protein interaction databases

BioGridi32995, 358 interactors
ComplexPortaliCPX-1947 Methionyl glutamyl tRNA synthetase complex
DIPiDIP-2212N
IntActiP46655, 46 interactors
MINTiP46655
STRINGi4932.YGL245W

Structurei

Secondary structure

1708
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP46655
SMRiP46655
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46655

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni106 – 115Interaction with ARC110
Regioni141 – 157Interaction with ARC1Add BLAST17
Regioni205 – 207Glutamate bindingBy similarity3
Regioni382 – 386Glutamate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi210 – 219"HIGH" region10
Motifi437 – 441"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00910000146834
HOGENOMiHOG000259234
InParanoidiP46655
KOiK01885
OMAiFARMNFI
OrthoDBiEOG092C0OTH

Family and domain databases

Gene3Di2.40.240.10, 3 hits
3.40.50.620, 1 hit
HAMAPiMF_02076 Glu_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR004526 Glu-tRNA-synth_arc/euk
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020059 Glu/Gln-tRNA-synth_Ib_codon-bd
IPR036282 Glutathione-S-Trfase_C_sf
IPR020056 Rbsml_L25/Gln-tRNA_synth_N
IPR011035 Ribosomal_L25/Gln-tRNA_synth
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PF03950 tRNA-synt_1c_C, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF47616 SSF47616, 1 hit
SSF50715 SSF50715, 1 hit
TIGRFAMsiTIGR00463 gltX_arch, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

P46655-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPSTLTINGK APIVAYAELI AARIVNALAP NSIAIKLVDD KKAPAAKLDD
60 70 80 90 100
ATEDVFNKIT SKFAAIFDNG DKEQVAKWVN LAQKELVIKN FAKLSQSLET
110 120 130 140 150
LDSQLNLRTF ILGGLKYSAA DVACWGALRS NGMCGSIIKN KVDVNVSRWY
160 170 180 190 200
TLLEMDPIFG EAHDFLSKSL LELKKSANVG KKKETHKANF EIDLPDAKMG
210 220 230 240 250
EVVTRFPPEP SGYLHIGHAK AALLNQYFAQ AYKGKLIIRF DDTNPSKEKE
260 270 280 290 300
EFQDSILEDL DLLGIKGDRI TYSSDYFQEM YDYCVQMIKD GKAYCDDTPT
310 320 330 340 350
EKMREERMDG VASARRDRSV EENLRIFTEE MKNGTEEGLK NCVRAKIDYK
360 370 380 390 400
ALNKTLRDPV IYRCNLTPHH RTGSTWKIYP TYDFCVPIVD AIEGVTHALR
410 420 430 440 450
TIEYRDRNAQ YDWMLQALRL RKVHIWDFAR INFVRTLLSK RKLQWMVDKD
460 470 480 490 500
LVGNWDDPRF PTVRGVRRRG MTVEGLRNFV LSQGPSRNVI NLEWNLIWAF
510 520 530 540 550
NKKVIDPIAP RHTAIVNPVK IHLEGSEAPQ EPKIEMKPKH KKNPAVGEKK
560 570 580 590 600
VIYYKDIVVD KDDADVINVD EEVTLMDWGN VIITKKNDDG SMVAKLNLEG
610 620 630 640 650
DFKKTKHKLT WLADTKDVVP VDLVDFDHLI TKDRLEEDES FEDFLTPQTE
660 670 680 690 700
FHTDAIADLN VKDMKIGDII QFERKGYYRL DALPKDGKPY VFFTIPDGKS

VNKYGAKK
Length:708
Mass (Da):80,843
Last modified:September 5, 2006 - v3
Checksum:iEADAD430A2D98974
GO

Sequence cautioni

The sequence AAA78905 differs from that shown. Reason: Frameshift at position 698.Curated
The sequence AAA78905 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA64142 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA89009 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA96964 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti209E → D in AAA78905 (Ref. 1) Curated1
Sequence conflicti473V → A in AAA78905 (Ref. 1) Curated1
Sequence conflicti510P → S in AAA78905 (Ref. 1) Curated1
Sequence conflicti546V → M in AAA78905 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32265 Genomic DNA Translation: AAA78905.1 Sequence problems.
Z49149 Genomic DNA Translation: CAA89009.1 Different initiation.
Z72767 Genomic DNA Translation: CAA96964.1 Different initiation.
X94357 Genomic DNA Translation: CAA64142.1 Different initiation.
BK006941 Genomic DNA Translation: DAA07874.1
PIRiS53934
RefSeqiNP_011269.2, NM_001181111.1

Genome annotation databases

EnsemblFungiiYGL245W; YGL245W; YGL245W
GeneIDi852606
KEGGisce:YGL245W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32265 Genomic DNA Translation: AAA78905.1 Sequence problems.
Z49149 Genomic DNA Translation: CAA89009.1 Different initiation.
Z72767 Genomic DNA Translation: CAA96964.1 Different initiation.
X94357 Genomic DNA Translation: CAA64142.1 Different initiation.
BK006941 Genomic DNA Translation: DAA07874.1
PIRiS53934
RefSeqiNP_011269.2, NM_001181111.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HRAX-ray1.90A/B1-191[»]
2HRKX-ray2.05A1-191[»]
2HSMX-ray3.00A1-191[»]
ProteinModelPortaliP46655
SMRiP46655
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32995, 358 interactors
ComplexPortaliCPX-1947 Methionyl glutamyl tRNA synthetase complex
DIPiDIP-2212N
IntActiP46655, 46 interactors
MINTiP46655
STRINGi4932.YGL245W

PTM databases

iPTMnetiP46655

Proteomic databases

MaxQBiP46655
PaxDbiP46655
PRIDEiP46655

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL245W; YGL245W; YGL245W
GeneIDi852606
KEGGisce:YGL245W

Organism-specific databases

EuPathDBiFungiDB:YGL245W
SGDiS000003214 GUS1

Phylogenomic databases

GeneTreeiENSGT00910000146834
HOGENOMiHOG000259234
InParanoidiP46655
KOiK01885
OMAiFARMNFI
OrthoDBiEOG092C0OTH

Enzyme and pathway databases

BioCyciYEAST:G3O-30716-MONOMER
BRENDAi6.1.1.17 984
SABIO-RKiP46655

Miscellaneous databases

EvolutionaryTraceiP46655
PROiPR:P46655

Family and domain databases

Gene3Di2.40.240.10, 3 hits
3.40.50.620, 1 hit
HAMAPiMF_02076 Glu_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR004526 Glu-tRNA-synth_arc/euk
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020059 Glu/Gln-tRNA-synth_Ib_codon-bd
IPR036282 Glutathione-S-Trfase_C_sf
IPR020056 Rbsml_L25/Gln-tRNA_synth_N
IPR011035 Ribosomal_L25/Gln-tRNA_synth
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PF03950 tRNA-synt_1c_C, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF47616 SSF47616, 1 hit
SSF50715 SSF50715, 1 hit
TIGRFAMsiTIGR00463 gltX_arch, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSYEC_YEAST
AccessioniPrimary (citable) accession number: P46655
Secondary accession number(s): D6VV90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 5, 2006
Last modified: September 12, 2018
This is version 176 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names
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Main funding by: National Institutes of Health

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