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UniProtKB - P46646 (AAT4_ARATH)
Protein
Aspartate aminotransferase, cytoplasmic isozyme 2
Gene
ASP4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Important for the metabolism of amino acids and Krebs-cycle related organic acids. In plants, it is involved in nitrogen metabolism and in aspects of carbon and energy metabolism.
Miscellaneous
In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.
Catalytic activityi
- EC:2.6.1.1
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 37 | Aspartate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 132 | AspartateBy similarity | 1 | |
Binding sitei | 185 | AspartateBy similarity | 1 | |
Binding sitei | 377 | AspartateBy similarity | 1 |
GO - Molecular functioni
- L-aspartate:2-oxoglutarate aminotransferase activity Source: UniProtKB
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- 2-oxoglutarate metabolic process Source: UniProtKB
- aspartate metabolic process Source: UniProtKB
- biosynthetic process Source: InterPro
- glutamate metabolic process Source: UniProtKB
Keywordsi
Molecular function | Aminotransferase, Transferase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | ARA:AT1G62800-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate aminotransferase, cytoplasmic isozyme 2 (EC:2.6.1.1)Alternative name(s): Transaminase A |
Gene namesi | Name:ASP4 Ordered Locus Names:At1g62800 ORF Names:F23N19.17, F23N19_26 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G62800 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Mitochondrion
- mitochondrion Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000123873 | 1 – 403 | Aspartate aminotransferase, cytoplasmic isozyme 2Add BLAST | 403 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 249 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | P46646 |
ProteomicsDBi | 245170 [P46646-1] |
Expressioni
Gene expression databases
ExpressionAtlasi | P46646, baseline and differential |
Genevisiblei | P46646, AT |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 3702.AT1G62800.2 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | KOG1411, Eukaryota |
HOGENOMi | CLU_032440_1_2_1 |
InParanoidi | P46646 |
OMAi | TGANHMA |
PhylomeDBi | P46646 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P46646-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNSILSSVLP APEDPVLSVI FACRDDPSPV KLNLSAGTYR TEEGKPLVLD
60 70 80 90 100
VVRRAEQQLA NDLDKEYLPL NGLPEFNKLS TKLILGDDSP ALKENRVVTT
110 120 130 140 150
QCLSGTGSLR VGAEFLATHN KESVIFVPNP TWGNHPRIFT LAGLSVQYFR
160 170 180 190 200
YYDPKSRGLD FKGMLEDLGA APPGAIVVLQ ACAHNPTGVD PTFEQWEKIR
210 220 230 240 250
RLVRSKSLLP FFDSAYQGFA SGSLDADAQA VRMFVADGGE CLIAQSYAKN
260 270 280 290 300
MGLYGERIGS LTIVCTSEDV AKKVENQVLL VVRPMYLTPP IHGASIVATI
310 320 330 340 350
LKNSDMYNDW TIELKGMADR IISMRQQLYA ALEARGTPGD WSHIIKHIGM
360 370 380 390 400
FTFTGLSEEQ VRLMAKEYHI YMTYDGRISM ASLSSKTVPQ LADAIHAVVT
RIA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF4I0D4 | F4I0D4_ARATH | Aspartate aminotransferase | ASP4 ASPARTATE AMINOTRANSFERASE 4, aspartate aminotransferase 4, At1g62800, F23N19.17, F23N19_17 | 405 | Annotation score: | ||
A0A1P8APT9 | A0A1P8APT9_ARATH | Aspartate aminotransferase | ASP4 ASPARTATE AMINOTRANSFERASE 4, aspartate aminotransferase 4, At1g62800, F23N19.17, F23N19_17 | 340 | Annotation score: |
Sequence cautioni
The sequence AAF19543 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 199 | I → F in AAA79372 (PubMed:7894512).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U15035 mRNA Translation: AAA79372.1 AC007190 Genomic DNA Translation: AAF19543.1 Sequence problems. CP002684 Genomic DNA Translation: AEE34006.1 |
PIRi | H96652 |
RefSeqi | NP_564803.1, NM_104958.2 [P46646-1] |
Genome annotation databases
EnsemblPlantsi | AT1G62800.1; AT1G62800.1; AT1G62800 [P46646-1] |
GeneIDi | 842579 |
Gramenei | AT1G62800.1; AT1G62800.1; AT1G62800 [P46646-1] |
KEGGi | ath:AT1G62800 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U15035 mRNA Translation: AAA79372.1 AC007190 Genomic DNA Translation: AAF19543.1 Sequence problems. CP002684 Genomic DNA Translation: AEE34006.1 |
PIRi | H96652 |
RefSeqi | NP_564803.1, NM_104958.2 [P46646-1] |
3D structure databases
SMRi | P46646 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT1G62800.2 |
Proteomic databases
PaxDbi | P46646 |
ProteomicsDBi | 245170 [P46646-1] |
Genome annotation databases
EnsemblPlantsi | AT1G62800.1; AT1G62800.1; AT1G62800 [P46646-1] |
GeneIDi | 842579 |
Gramenei | AT1G62800.1; AT1G62800.1; AT1G62800 [P46646-1] |
KEGGi | ath:AT1G62800 |
Organism-specific databases
Araporti | AT1G62800 |
Phylogenomic databases
eggNOGi | KOG1411, Eukaryota |
HOGENOMi | CLU_032440_1_2_1 |
InParanoidi | P46646 |
OMAi | TGANHMA |
PhylomeDBi | P46646 |
Enzyme and pathway databases
BioCyci | ARA:AT1G62800-MONOMER |
Miscellaneous databases
PROi | PR:P46646 |
Gene expression databases
ExpressionAtlasi | P46646, baseline and differential |
Genevisiblei | P46646, AT |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AAT4_ARATH | |
Accessioni | P46646Primary (citable) accession number: P46646 Secondary accession number(s): Q9SI69 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | June 20, 2002 | |
Last modified: | February 23, 2022 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families