UniProtKB - P46597 (ASMT_HUMAN)
Acetylserotonin O-methyltransferase
ASMT
Functioni
Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).
1 PublicationDoes not show Acetylserotonin O-methyltransferase activity.
1 PublicationDoes not show Acetylserotonin O-methyltransferase activity.
1 PublicationMiscellaneous
Catalytic activityi
- N-acetylserotonin + S-adenosyl-L-methionine = H+ + melatonin + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.41 PublicationThis reaction proceeds in the backward1 Publication direction.
: melatonin biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes melatonin from serotonin.1 Publication This subpathway is part of the pathway melatonin biosynthesis, which is itself part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes melatonin from serotonin, the pathway melatonin biosynthesis and in Aromatic compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 147 | S-adenosyl-L-methionine | 1 | |
Binding sitei | 164 | S-adenosyl-L-methionine | 1 | |
Binding sitei | 210 | S-adenosyl-L-methionine | 1 | |
Binding sitei | 252 | S-adenosyl-L-methionine | 1 | |
Active sitei | 255 | Proton donor/acceptor1 Publication | 1 | |
Binding sitei | 256 | Substrate | 1 | |
Binding sitei | 302 | Substrate | 1 | |
Binding sitei | 306 | Substrate | 1 |
GO - Molecular functioni
- acetylserotonin O-methyltransferase activity Source: UniProtKB
- identical protein binding Source: IntAct
- O-methyltransferase activity Source: GO_Central
- protein homodimerization activity Source: UniProtKB
- S-adenosylmethionine-dependent methyltransferase activity Source: GO_Central
- S-methyltransferase activity Source: Reactome
GO - Biological processi
- aromatic compound biosynthetic process Source: GO_Central
- indolalkylamine biosynthetic process Source: Reactome
- lipid metabolic process Source: UniProtKB-KW
- melatonin biosynthetic process Source: UniProtKB
- methylation Source: GO_Central
- translation Source: ProtInc
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Lipid metabolism, Melatonin biosynthesis |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.4, 2681 |
PathwayCommonsi | P46597 |
Reactomei | R-HSA-209931, Serotonin and melatonin biosynthesis |
SIGNORi | P46597 |
UniPathwayi | UPA00837;UER00815 |
Names & Taxonomyi
Protein namesi | Recommended name: Acetylserotonin O-methyltransferase (EC:2.1.1.41 Publication)Alternative name(s): Hydroxyindole O-methyltransferase Short name: HIOMT |
Gene namesi | Name:ASMT |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:750, ASMT |
MIMi | 300015, gene 402500, gene |
neXtProti | NX_P46597 |
VEuPathDBi | HostDB:ENSG00000196433 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 11 | L → F: Reduced enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 31 | L → H: No effect on enzyme activity. | 1 | |
Mutagenesisi | 111 | R → K: No effect on enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 248 | Y → H: Nearly abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 296 | T → M: Nearly abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 318 | H → D: Reduced enzyme activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 438 |
OpenTargetsi | ENSG00000196433 |
PharmGKBi | PA25049 |
Miscellaneous databases
Pharosi | P46597, Tbio |
Chemistry databases
DrugBanki | DB01065, Melatonin |
Genetic variation databases
BioMutai | ASMT |
DMDMi | 1170276 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083982 | 1 – 345 | Acetylserotonin O-methyltransferaseAdd BLAST | 345 |
Proteomic databases
MaxQBi | P46597 |
PeptideAtlasi | P46597 |
PRIDEi | P46597 |
ProteomicsDBi | 55743 [P46597-1] 55744 [P46597-2] 55745 [P46597-3] |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000196433, Expressed in muscle tissue and 99 other tissues |
ExpressionAtlasi | P46597, baseline and differential |
Genevisiblei | P46597, HS |
Organism-specific databases
HPAi | ENSG00000196433, Tissue enhanced (brain) |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
P46597
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-6502097,EBI-6502097 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 106930, 1 interactor |
STRINGi | 9606.ENSP00000370639 |
Miscellaneous databases
RNActi | P46597, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P46597 |
SMRi | P46597 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 235 – 237 | S-adenosyl-L-methionine binding | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3178, Eukaryota |
GeneTreei | ENSGT00940000161561 |
HOGENOMi | CLU_005533_4_2_1 |
InParanoidi | P46597 |
OMAi | EYMEGFL |
PhylomeDBi | P46597 |
TreeFami | TF314574 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 3.40.50.150, 1 hit |
InterProi | View protein in InterPro IPR031725, ASMT_dimerisation IPR016461, COMT-like IPR001077, O_MeTrfase_dom IPR029063, SAM-dependent_MTases IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11746, PTHR11746, 1 hit |
Pfami | View protein in Pfam PF16864, Dimerisation2, 1 hit PF00891, Methyltransf_2, 1 hit |
PIRSFi | PIRSF005739, O-mtase, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51683, SAM_OMT_II, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGSSEDQAYR LLNDYANGFM VSQVLFAACE LGVFDLLAEA PGPLDVAAVA
60 70 80 90 100
AGVRASAHGT ELLLDICVSL KLLKVETRGG KAFYRNTELS SDYLTTVSPT
110 120 130 140 150
SQCSMLKYMG RTSYRCWGHL ADAVREGRNQ YLETFGVPAE ELFTAIYRSE
160 170 180 190 200
GERLQFMQAL QEVWSVNGRS VLTAFDLSVF PLMCDLGGGA GALAKECMSL
210 220 230 240 250
YPGCKITVFD IPEVVWTAKQ HFSFQEEEQI DFQEGDFFKD PLPEADLYIL
260 270 280 290 300
ARVLHDWADG KCSHLLERIY HTCKPGGGIL VIESLLDEDR RGPLLTQLYS
310 320 330 340
LNMLVQTEGQ ERTPTHYHML LSSAGFRDFQ FKKTGAIYDA ILARK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketI7HFW6 | I7HFW6_HUMAN | Acetylserotonin O-methyltransferase | ASMT | 124 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 302 | N → S in BAG37430 (PubMed:14702039).Curated | 1 | ||
Isoform 3 (identifier: P46597-3) | |||||
Sequence conflicti | 190 | W → R in AAA58582 (PubMed:7989373).Curated | 1 | ||
Sequence conflicti | 190 | W → R in AAA58583 (PubMed:7989373).Curated | 1 | ||
Sequence conflicti | 190 | W → R in AAA75290 (PubMed:7989373).Curated | 1 | ||
Sequence conflicti | 190 | W → R in AAA17020 (PubMed:8397829).Curated | 1 | ||
Sequence conflicti | 190 | W → R in BAG37430 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_069111 | 13 | N → H No effect on enzyme activity. 2 PublicationsCorresponds to variant dbSNP:rs121918819Ensembl. | 1 | |
Natural variantiVAR_045991 | 17 | N → K Nearly abolishes enzyme activity. 4 PublicationsCorresponds to variant dbSNP:rs17149149Ensembl. | 1 | |
Natural variantiVAR_069112 | 61 | E → Q Reduced enzyme activity. 3 PublicationsCorresponds to variant dbSNP:rs121918823Ensembl. | 1 | |
Natural variantiVAR_069114 | 81 | K → E No effect on enzyme activity. 2 PublicationsCorresponds to variant dbSNP:rs117343570EnsemblClinVar. | 1 | |
Natural variantiVAR_069115 | 115 | R → W1 PublicationCorresponds to variant dbSNP:rs201053197Ensembl. | 1 | |
Natural variantiVAR_069116 | 151 | G → S1 PublicationCorresponds to variant dbSNP:rs192710293Ensembl. | 1 | |
Natural variantiVAR_069117 | 166 | V → I1 PublicationCorresponds to variant dbSNP:rs373339042Ensembl. | 1 | |
Natural variantiVAR_069118 | 171 | V → M Nearly abolishes enzyme activity. 2 PublicationsCorresponds to variant dbSNP:rs121918820Ensembl. | 1 | |
Natural variantiVAR_069119 | 179 | V → G1 PublicationCorresponds to variant dbSNP:rs1215166326Ensembl. | 1 | |
Natural variantiVAR_069120 | 210 | D → G Nearly abolishes enzyme activity. 3 PublicationsCorresponds to variant dbSNP:rs121918824Ensembl. | 1 | |
Natural variantiVAR_069121 | 211 | I → M1 PublicationCorresponds to variant dbSNP:rs201316181Ensembl. | 1 | |
Natural variantiVAR_069122 | 217 | T → M1 PublicationCorresponds to variant dbSNP:rs148036160Ensembl. | 1 | |
Natural variantiVAR_069123 | 219 | K → R No effect on enzyme activity. 3 PublicationsCorresponds to variant dbSNP:rs121918825Ensembl. | 1 | |
Natural variantiVAR_069125 | 243 | P → L Reduced enzyme activity. 4 PublicationsCorresponds to variant dbSNP:rs121918826Ensembl. | 1 | |
Natural variantiVAR_069126 | 269 | I → M Reduced enzyme activity. 1 PublicationCorresponds to variant dbSNP:rs146121655Ensembl. | 1 | |
Natural variantiVAR_069127 | 273 | C → S Reduced enzyme activity. 3 PublicationsCorresponds to variant dbSNP:rs121918827Ensembl. | 1 | |
Natural variantiVAR_069128 | 278 | G → A Reduced enzyme activity. 2 PublicationsCorresponds to variant dbSNP:rs1188440875Ensembl. | 1 | |
Natural variantiVAR_069129 | 288 | E → D No effect on enzyme activity. 4 PublicationsCorresponds to variant dbSNP:rs121918821Ensembl. | 1 | |
Natural variantiVAR_069130 | 291 | R → Q Nearly abolishes enzyme activity. 3 PublicationsCorresponds to variant dbSNP:rs121918828Ensembl. | 1 | |
Natural variantiVAR_069131 | 298 | L → F Found in patients with neuropsychiatric disorders; unknown pathological significance; nearly abolishes enzyme activity. 6 PublicationsCorresponds to variant dbSNP:rs121918822Ensembl. | 1 | |
Natural variantiVAR_069132 | 305 | V → M Found in a patient with bipolar disorder; unknown pathological significance; reduced enzyme activity. 2 Publications | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004284 | 188 | G → GTWIKLETIILSKLSQGQKT KHRVFSLIG in isoform 3. 2 Publications | 1 | |
Alternative sequenceiVSP_004285 | 189 – 235 | Missing in isoform 2. 1 PublicationAdd BLAST | 47 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U11098 , U11089, U11093, U11094, U11095, U11096, U11092, U11097 Genomic DNA Translation: AAA75291.1 U11098 , U11089, U11093, U11094, U11095, U11096, U11097 Genomic DNA Translation: AAA75289.1 U11098 , U11089, U11093, U11094, U11095, U11096, U11092, U11097 Genomic DNA Translation: AAA75290.1 U11090 mRNA Translation: AAA58582.1 U11091 mRNA Translation: AAA58583.1 M83779 mRNA Translation: AAA17020.1 AK314922 mRNA Translation: BAG37430.1 AL683807 Genomic DNA No translation available. BC001620 mRNA Translation: AAH01620.1 |
CCDSi | CCDS14117.1 [P46597-3] CCDS55364.1 [P46597-2] |
PIRi | I37463 |
RefSeqi | NP_001164509.1, NM_001171038.1 [P46597-3] NP_001164510.1, NM_001171039.1 [P46597-2] NP_004034.2, NM_004043.2 [P46597-3] |
Genome annotation databases
Ensembli | ENST00000381229.9; ENSP00000370627.4; ENSG00000196433.13 ENST00000381233.8; ENSP00000370631.3; ENSG00000196433.13 [P46597-2] ENST00000381241.9; ENSP00000370639.3; ENSG00000196433.13 [P46597-3] |
GeneIDi | 438 |
KEGGi | hsa:438 |
MANE-Selecti | ENST00000381241.9; ENSP00000370639.3; NM_001171038.2; NP_001164509.1 [P46597-3] |
UCSCi | uc010ncy.4, human [P46597-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia 5-hydroxyindole-O-methyltransferase entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U11098 , U11089, U11093, U11094, U11095, U11096, U11092, U11097 Genomic DNA Translation: AAA75291.1 U11098 , U11089, U11093, U11094, U11095, U11096, U11097 Genomic DNA Translation: AAA75289.1 U11098 , U11089, U11093, U11094, U11095, U11096, U11092, U11097 Genomic DNA Translation: AAA75290.1 U11090 mRNA Translation: AAA58582.1 U11091 mRNA Translation: AAA58583.1 M83779 mRNA Translation: AAA17020.1 AK314922 mRNA Translation: BAG37430.1 AL683807 Genomic DNA No translation available. BC001620 mRNA Translation: AAH01620.1 |
CCDSi | CCDS14117.1 [P46597-3] CCDS55364.1 [P46597-2] |
PIRi | I37463 |
RefSeqi | NP_001164509.1, NM_001171038.1 [P46597-3] NP_001164510.1, NM_001171039.1 [P46597-2] NP_004034.2, NM_004043.2 [P46597-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4A6D | X-ray | 2.40 | A | 1-345 | [»] | |
4A6E | X-ray | 2.70 | A | 1-345 | [»] | |
AlphaFoldDBi | P46597 | |||||
SMRi | P46597 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106930, 1 interactor |
STRINGi | 9606.ENSP00000370639 |
Chemistry databases
DrugBanki | DB01065, Melatonin |
Genetic variation databases
BioMutai | ASMT |
DMDMi | 1170276 |
Proteomic databases
MaxQBi | P46597 |
PeptideAtlasi | P46597 |
PRIDEi | P46597 |
ProteomicsDBi | 55743 [P46597-1] 55744 [P46597-2] 55745 [P46597-3] |
Protocols and materials databases
Antibodypediai | 35250, 173 antibodies from 24 providers |
DNASUi | 438 |
Genome annotation databases
Ensembli | ENST00000381229.9; ENSP00000370627.4; ENSG00000196433.13 ENST00000381233.8; ENSP00000370631.3; ENSG00000196433.13 [P46597-2] ENST00000381241.9; ENSP00000370639.3; ENSG00000196433.13 [P46597-3] |
GeneIDi | 438 |
KEGGi | hsa:438 |
MANE-Selecti | ENST00000381241.9; ENSP00000370639.3; NM_001171038.2; NP_001164509.1 [P46597-3] |
UCSCi | uc010ncy.4, human [P46597-1] |
Organism-specific databases
CTDi | 438 |
DisGeNETi | 438 |
GeneCardsi | ASMT |
HGNCi | HGNC:750, ASMT |
HPAi | ENSG00000196433, Tissue enhanced (brain) |
MIMi | 300015, gene 402500, gene |
neXtProti | NX_P46597 |
OpenTargetsi | ENSG00000196433 |
PharmGKBi | PA25049 |
VEuPathDBi | HostDB:ENSG00000196433 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3178, Eukaryota |
GeneTreei | ENSGT00940000161561 |
HOGENOMi | CLU_005533_4_2_1 |
InParanoidi | P46597 |
OMAi | EYMEGFL |
PhylomeDBi | P46597 |
TreeFami | TF314574 |
Enzyme and pathway databases
UniPathwayi | UPA00837;UER00815 |
BRENDAi | 2.1.1.4, 2681 |
PathwayCommonsi | P46597 |
Reactomei | R-HSA-209931, Serotonin and melatonin biosynthesis |
SIGNORi | P46597 |
Miscellaneous databases
BioGRID-ORCSi | 438, 6 hits in 566 CRISPR screens |
ChiTaRSi | ASMT, human |
GeneWikii | Acetylserotonin_O-methyltransferase |
GenomeRNAii | 438 |
Pharosi | P46597, Tbio |
PROi | PR:P46597 |
RNActi | P46597, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000196433, Expressed in muscle tissue and 99 other tissues |
ExpressionAtlasi | P46597, baseline and differential |
Genevisiblei | P46597, HS |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 3.40.50.150, 1 hit |
InterProi | View protein in InterPro IPR031725, ASMT_dimerisation IPR016461, COMT-like IPR001077, O_MeTrfase_dom IPR029063, SAM-dependent_MTases IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11746, PTHR11746, 1 hit |
Pfami | View protein in Pfam PF16864, Dimerisation2, 1 hit PF00891, Methyltransf_2, 1 hit |
PIRSFi | PIRSF005739, O-mtase, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51683, SAM_OMT_II, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ASMT_HUMAN | |
Accessioni | P46597Primary (citable) accession number: P46597 Secondary accession number(s): B2RC33 Q5JQ73 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families