UniProtKB - P46460 (NSF_MOUSE)
Protein
Vesicle-fusing ATPase
Gene
Nsf
Organism
Mus musculus (Mouse)
Status
Functioni
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack GRIA2 leads to influence GRIA2 membrane cycling (By similarity).By similarity1 Publication
Catalytic activityi
- EC:3.6.4.6
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 550 | MagnesiumBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 505 – 510 | ATPBy similarity | 6 | |
Nucleotide bindingi | 545 – 552 | ATPBy similarity | 8 |
GO - Molecular functioni
- ATPase activity Source: MGI
- ATP binding Source: UniProtKB-KW
- ATP-dependent protein binding Source: MGI
- D1 dopamine receptor binding Source: MGI
- ionotropic glutamate receptor binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- PDZ domain binding Source: MGI
- protein-containing complex binding Source: ParkinsonsUK-UCL
- protein C-terminus binding Source: MGI
- protein kinase binding Source: ParkinsonsUK-UCL
- Rab GTPase binding Source: MGI
- SNARE binding Source: ParkinsonsUK-UCL
- syntaxin-1 binding Source: ParkinsonsUK-UCL
- syntaxin binding Source: MGI
GO - Biological processi
- Golgi to plasma membrane protein transport Source: GO_Central
- Golgi vesicle docking Source: GO_Central
- intracellular protein transport Source: MGI
- intra-Golgi vesicle-mediated transport Source: GO_Central
- positive regulation of protein catabolic process Source: MGI
- positive regulation of receptor recycling Source: MGI
- potassium ion transport Source: MGI
- protein-containing complex disassembly Source: MGI
- protein transport Source: MGI
- regulation of exocytosis Source: MGI
- regulation of receptor internalization Source: MGI
- SNARE complex disassembly Source: ParkinsonsUK-UCL
- vesicle-mediated transport Source: MGI
Keywordsi
Molecular function | Hydrolase |
Biological process | Protein transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-204005, COPII-mediated vesicle transport R-MMU-416993, Trafficking of GluR2-containing AMPA receptors R-MMU-6807878, COPI-mediated anterograde transport R-MMU-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-MMU-6811438, Intra-Golgi traffic R-MMU-6811440, Retrograde transport at the Trans-Golgi-Network |
Names & Taxonomyi
Protein namesi | Recommended name: Vesicle-fusing ATPase (EC:3.6.4.6)Alternative name(s): N-ethylmaleimide-sensitive fusion protein Short name: NEM-sensitive fusion protein Suppressor of K(+) transport growth defect 2 Short name: Protein SKD2 Vesicular-fusion protein NSF |
Gene namesi | Name:Nsf Synonyms:Skd2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104560, Nsf |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: MGI
Golgi apparatus
- Golgi stack Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- dendritic shaft Source: MGI
- myelin sheath Source: UniProtKB
- postsynaptic density Source: MGI
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 569 | S → A: Abolishes phosphorylation by CDK16. 1 Publication | 1 | |
Mutagenesisi | 569 | S → E: Interferes with oligomerization. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084564 | 1 – 744 | Vesicle-fusing ATPaseAdd BLAST | 744 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 105 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 207 | PhosphoserineCombined sources | 1 | |
Modified residuei | 259 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 569 | Phosphoserine; by CDK161 Publication | 1 |
Post-translational modificationi
Phosphorylation at Ser-569 interferes with homohexamerization.1 Publication
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P46460 |
jPOSTi | P46460 |
MaxQBi | P46460 |
PaxDbi | P46460 |
PeptideAtlasi | P46460 |
PRIDEi | P46460 |
2D gel databases
REPRODUCTION-2DPAGEi | P46460 |
PTM databases
iPTMneti | P46460 |
PhosphoSitePlusi | P46460 |
SwissPalmi | P46460 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000034187, Expressed in dentate gyrus of hippocampal formation granule cell and 308 other tissues |
ExpressionAtlasi | P46460, baseline and differential |
Genevisiblei | P46460, MM |
Interactioni
Subunit structurei
Homohexamer.
Interacts with GABARAP and GABARAPL2.
Interacts with GRIA2.
Interacts with PLK2, leading to disrupt the interaction with GRIA2.
Interacts with MUSK; may regulate MUSK endocytosis and activity (By similarity).
Interacts with CDK16.
By similarity2 PublicationsBinary interactionsi
Hide detailsP46460
With | #Exp. | IntAct |
---|---|---|
Dlg4 [Q62108] | 5 | EBI-398006,EBI-300895 |
Lrrk2 [Q5S006] | 3 | EBI-398006,EBI-2693710 |
Musk - isoform 3 [Q61006-3] | 5 | EBI-398006,EBI-6308424 |
Ptpn9 [O35239] | 2 | EBI-398006,EBI-7297868 |
GO - Molecular functioni
- ATP-dependent protein binding Source: MGI
- D1 dopamine receptor binding Source: MGI
- ionotropic glutamate receptor binding Source: MGI
- PDZ domain binding Source: MGI
- protein C-terminus binding Source: MGI
- protein kinase binding Source: ParkinsonsUK-UCL
- Rab GTPase binding Source: MGI
- SNARE binding Source: ParkinsonsUK-UCL
- syntaxin-1 binding Source: ParkinsonsUK-UCL
- syntaxin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201857, 41 interactors |
IntActi | P46460, 27 interactors |
MINTi | P46460 |
STRINGi | 10090.ENSMUSP00000099364 |
Miscellaneous databases
RNActi | P46460, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the AAA ATPase family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0741, Eukaryota |
GeneTreei | ENSGT00530000064085 |
HOGENOMi | CLU_008037_2_0_1 |
InParanoidi | P46460 |
OMAi | RMRAIKC |
OrthoDBi | 197562at2759 |
PhylomeDBi | P46460 |
TreeFami | TF300371 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR009010, Asp_de-COase-like_dom_sf IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR004201, Cdc48_dom2 IPR029067, CDC48_domain_2-like_sf IPR003338, CDC4_N-term_subdom IPR027417, P-loop_NTPase IPR039812, Vesicle-fus_ATPase |
PANTHERi | PTHR23078, PTHR23078, 1 hit |
Pfami | View protein in Pfam PF00004, AAA, 2 hits PF17862, AAA_lid_3, 1 hit PF02933, CDC48_2, 1 hit PF02359, CDC48_N, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 2 hits SM01072, CDC48_2, 1 hit SM01073, CDC48_N, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit SSF52540, SSF52540, 2 hits SSF54585, SSF54585, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P46460-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGRTMQAAR CPTDELSLSN CAVVNEKDFQ SGQHVMVRTS PNHKYIFTLR
60 70 80 90 100
THPSVVPGCI AFSLPQRKWA GLSIGQDIEV ALYSFDKAKQ CIGTMTIEID
110 120 130 140 150
FLQKKNIDSN PYDTDKMAAE FIQQFNNQAF SVGQQLVFSF NDKLFGLLVK
160 170 180 190 200
DIEAMDPSIL KGEPASGKRQ KIEVGLVVGN SQVAFEKAEN SSLNLIGKAK
210 220 230 240 250
TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF PPEIVEQMGC
260 270 280 290 300
KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA
310 320 330 340 350
NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV
360 370 380 390 400
VNQLLSKIDG VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE
410 420 430 440 450
KGRLQILHIH TARMRGHQLL SADVDIKELA VETKNFSGAE LEGLVRAAQS
460 470 480 490 500
TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF LASLENDIKP AFGTNQEDYA
510 520 530 540 550
SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL LEGPPHSGKT
560 570 580 590 600
ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV
610 620 630 640 650
VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD
660 670 680 690 700
VLQEMEMLNA FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG
710 720 730 740
KKVWIGIKKL LMLIEMSLQM DPEYRVRKFL ALMREEGASP LDFD
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3UX86 | G3UX86_MOUSE | Vesicle-fusing ATPase | Nsf | 210 | Annotation score: | ||
G3UX98 | G3UX98_MOUSE | Vesicle-fusing ATPase | Nsf | 76 | Annotation score: |
Sequence cautioni
The sequence CAM20943 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM23742 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 300 | A → G in BAC39361 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 316 – 326 | LGANSGLHIII → VCGHKTNLLKM in BAC34637 (PubMed:16141072).CuratedAdd BLAST | 11 | |
Sequence conflicti | 492 | F → I in AAA50498 (PubMed:8082782).Curated | 1 | |
Sequence conflicti | 552 | L → V in AAA50498 (PubMed:8082782).Curated | 1 | |
Sequence conflicti | 657 | M → I in BAC25937 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10120 mRNA Translation: AAA50498.1 AK028415 mRNA Translation: BAC25937.1 AK032120 mRNA Translation: BAC27713.1 AK049281 mRNA Translation: BAC33656.1 AK051430 mRNA Translation: BAC34637.1 AK085086 mRNA Translation: BAC39361.1 AK153905 mRNA Translation: BAE32247.1 AL596108, AL603829 Genomic DNA Translation: CAM20943.1 Sequence problems. AL596108, AL603829 Genomic DNA Translation: CAM20945.1 AL603829, AL596108 Genomic DNA Translation: CAM23742.1 Sequence problems. AL603829, AL596108 Genomic DNA Translation: CAM23743.1 BC006627 mRNA Translation: AAH06627.1 BC019167 mRNA Translation: AAH19167.1 |
CCDSi | CCDS25524.1 |
RefSeqi | NP_032766.2, NM_008740.4 |
Genome annotation databases
Ensembli | ENSMUST00000103075; ENSMUSP00000099364; ENSMUSG00000034187 |
GeneIDi | 18195 |
KEGGi | mmu:18195 |
UCSCi | uc007lvt.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10120 mRNA Translation: AAA50498.1 AK028415 mRNA Translation: BAC25937.1 AK032120 mRNA Translation: BAC27713.1 AK049281 mRNA Translation: BAC33656.1 AK051430 mRNA Translation: BAC34637.1 AK085086 mRNA Translation: BAC39361.1 AK153905 mRNA Translation: BAE32247.1 AL596108, AL603829 Genomic DNA Translation: CAM20943.1 Sequence problems. AL596108, AL603829 Genomic DNA Translation: CAM20945.1 AL603829, AL596108 Genomic DNA Translation: CAM23742.1 Sequence problems. AL603829, AL596108 Genomic DNA Translation: CAM23743.1 BC006627 mRNA Translation: AAH06627.1 BC019167 mRNA Translation: AAH19167.1 |
CCDSi | CCDS25524.1 |
RefSeqi | NP_032766.2, NM_008740.4 |
3D structure databases
SMRi | P46460 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201857, 41 interactors |
IntActi | P46460, 27 interactors |
MINTi | P46460 |
STRINGi | 10090.ENSMUSP00000099364 |
PTM databases
iPTMneti | P46460 |
PhosphoSitePlusi | P46460 |
SwissPalmi | P46460 |
2D gel databases
REPRODUCTION-2DPAGEi | P46460 |
Proteomic databases
EPDi | P46460 |
jPOSTi | P46460 |
MaxQBi | P46460 |
PaxDbi | P46460 |
PeptideAtlasi | P46460 |
PRIDEi | P46460 |
Protocols and materials databases
Antibodypediai | 1006, 275 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000103075; ENSMUSP00000099364; ENSMUSG00000034187 |
GeneIDi | 18195 |
KEGGi | mmu:18195 |
UCSCi | uc007lvt.1, mouse |
Organism-specific databases
CTDi | 4905 |
MGIi | MGI:104560, Nsf |
Phylogenomic databases
eggNOGi | KOG0741, Eukaryota |
GeneTreei | ENSGT00530000064085 |
HOGENOMi | CLU_008037_2_0_1 |
InParanoidi | P46460 |
OMAi | RMRAIKC |
OrthoDBi | 197562at2759 |
PhylomeDBi | P46460 |
TreeFami | TF300371 |
Enzyme and pathway databases
Reactomei | R-MMU-204005, COPII-mediated vesicle transport R-MMU-416993, Trafficking of GluR2-containing AMPA receptors R-MMU-6807878, COPI-mediated anterograde transport R-MMU-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-MMU-6811438, Intra-Golgi traffic R-MMU-6811440, Retrograde transport at the Trans-Golgi-Network |
Miscellaneous databases
BioGRID-ORCSi | 18195, 8 hits in 19 CRISPR screens |
ChiTaRSi | Nsf, mouse |
PROi | PR:P46460 |
RNActi | P46460, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034187, Expressed in dentate gyrus of hippocampal formation granule cell and 308 other tissues |
ExpressionAtlasi | P46460, baseline and differential |
Genevisiblei | P46460, MM |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR009010, Asp_de-COase-like_dom_sf IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR004201, Cdc48_dom2 IPR029067, CDC48_domain_2-like_sf IPR003338, CDC4_N-term_subdom IPR027417, P-loop_NTPase IPR039812, Vesicle-fus_ATPase |
PANTHERi | PTHR23078, PTHR23078, 1 hit |
Pfami | View protein in Pfam PF00004, AAA, 2 hits PF17862, AAA_lid_3, 1 hit PF02933, CDC48_2, 1 hit PF02359, CDC48_N, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 2 hits SM01072, CDC48_2, 1 hit SM01073, CDC48_N, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit SSF52540, SSF52540, 2 hits SSF54585, SSF54585, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NSF_MOUSE | |
Accessioni | P46460Primary (citable) accession number: P46460 Secondary accession number(s): A2A646 Q923C6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | May 1, 2007 | |
Last modified: | December 2, 2020 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families