UniProtKB - P46422 (GSTF2_ARATH)
Glutathione S-transferase F2
GSTF2
Functioni
Binds auxin, endogenous flavonoids and the phytoalexin camalexin and may be involved in regulating the binding and transport of small bioactive natural products and defense-related compounds during plant stress. Binds a series of heterocyclic compounds, including lumichrome, harmane, norharmane and indole-3-aldehyde. In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC). Acts as glutathione peroxidase on cumene hydroperoxide, linoleic acid-13-hydroperoxide and trans-stilbene oxide, but not trans-cinnamic acid or IAA-CoA.
2 PublicationsCatalytic activityi
- EC:2.5.1.18
GO - Molecular functioni
- camalexin binding Source: TAIR
- glutathione binding Source: TAIR
- glutathione transferase activity Source: TAIR
- peroxidase activity Source: UniProtKB-KW
- protein domain specific binding Source: CAFA
- quercitrin binding Source: TAIR
- salicylic acid binding Source: TAIR
GO - Biological processi
- auxin-activated signaling pathway Source: UniProtKB-KW
- defense response Source: UniProtKB-KW
- glutathione metabolic process Source: InterPro
- response to cadmium ion Source: TAIR
- response to cold Source: TAIR
- response to oomycetes Source: TAIR
- response to zinc ion Source: TAIR
- toxin catabolic process Source: TAIR
Keywordsi
Molecular function | Oxidoreductase, Peroxidase, Transferase |
Biological process | Auxin signaling pathway, Detoxification, Plant defense, Stress response |
Enzyme and pathway databases
BioCyci | ARA:AT4G02520-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Glutathione S-transferase F2 (EC:2.5.1.18)Short name: AtGSTF2 Alternative name(s): 24 kDa auxin-binding protein Short name: AtPM24 GST class-phi member 2 |
Gene namesi | Name:GSTF2 Synonyms:PM24.1 Ordered Locus Names:At4g02520 ORF Names:T10P11.18 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G02520 |
TAIRi | locus:2132308, AT4G02520 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol 1 Publication
Endoplasmic reticulum
- Microsome 1 Publication
- Endoplasmic reticulum 1 Publication
Note: Plasma membrane vesicles.
Chloroplast
- chloroplast Source: TAIR
- chloroplast stroma Source: TAIR
Cytosol
- cytosol Source: TAIR
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Extracellular region or secreted
- apoplast Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Vacuole
- plant-type vacuole Source: TAIR
Other locations
- cytoplasm Source: GO_Central
- intracellular membrane-bounded organelle Source: TAIR
- plasmodesma Source: TAIR
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185848 | 1 – 212 | Glutathione S-transferase F2Add BLAST | 212 |
Proteomic databases
PaxDbi | P46422 |
PRIDEi | P46422 |
ProteomicsDBi | 230161 |
2D gel databases
SWISS-2DPAGEi | P46422 |
PTM databases
iPTMneti | P46422 |
MetOSitei | P46422 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
ExpressionAtlasi | P46422, baseline and differential |
Genevisiblei | P46422, AT |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsGO - Molecular functioni
- protein domain specific binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 13222, 8 interactors |
IntActi | P46422, 1 interactor |
STRINGi | 3702.AT4G02520.1 |
Structurei
Secondary structure
3D structure databases
SMRi | P46422 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P46422 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 83 | GST N-terminalAdd BLAST | 82 | |
Domaini | 93 – 212 | GST C-terminalAdd BLAST | 120 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 12 – 13 | Glutathione binding | 2 | |
Regioni | 41 – 42 | Glutathione binding | 2 | |
Regioni | 54 – 55 | Glutathione binding | 2 | |
Regioni | 67 – 68 | Glutathione binding | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0867, Eukaryota |
HOGENOMi | CLU_011226_5_1_1 |
InParanoidi | P46422 |
OMAi | DYLAGNN |
OrthoDBi | 1231780at2759 |
PhylomeDBi | P46422 |
Family and domain databases
CDDi | cd03187, GST_C_Phi, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR004045, Glutathione_S-Trfase_N IPR004046, GST_C IPR034347, GST_Phi_C IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF00043, GST_C, 1 hit PF02798, GST_N, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAGIKVFGHP ASIATRRVLI ALHEKNLDFE LVHVELKDGE HKKEPFLSRN
60 70 80 90 100
PFGQVPAFED GDLKLFESRA ITQYIAHRYE NQGTNLLQTD SKNISQYAIM
110 120 130 140 150
AIGMQVEDHQ FDPVASKLAF EQIFKSIYGL TTDEAVVAEE EAKLAKVLDV
160 170 180 190 200
YEARLKEFKY LAGETFTLTD LHHIPAIQYL LGTPTKKLFT ERPRVNEWVA
210
EITKRPASEK VQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X75303 mRNA Translation: CAA53051.1 L07589 mRNA Translation: AAA32800.1 L11601 mRNA Translation: AAA32801.1 AC002330 Genomic DNA Translation: AAC78264.1 AL161494 Genomic DNA Translation: CAB80745.1 CP002687 Genomic DNA Translation: AEE82183.1 AF324681 mRNA Translation: AAG40032.1 AF326903 mRNA Translation: AAG41485.1 AF349527 mRNA Translation: AAK15574.1 AY039580 mRNA Translation: AAK62635.1 AY056082 mRNA Translation: AAL06970.1 |
PIRi | S35268 |
RefSeqi | NP_192161.1, NM_116486.3 |
Genome annotation databases
EnsemblPlantsi | AT4G02520.1; AT4G02520.1; AT4G02520 |
GeneIDi | 827931 |
Gramenei | AT4G02520.1; AT4G02520.1; AT4G02520 |
KEGGi | ath:AT4G02520 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X75303 mRNA Translation: CAA53051.1 L07589 mRNA Translation: AAA32800.1 L11601 mRNA Translation: AAA32801.1 AC002330 Genomic DNA Translation: AAC78264.1 AL161494 Genomic DNA Translation: CAB80745.1 CP002687 Genomic DNA Translation: AEE82183.1 AF324681 mRNA Translation: AAG40032.1 AF326903 mRNA Translation: AAG41485.1 AF349527 mRNA Translation: AAK15574.1 AY039580 mRNA Translation: AAK62635.1 AY056082 mRNA Translation: AAL06970.1 |
PIRi | S35268 |
RefSeqi | NP_192161.1, NM_116486.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BX9 | X-ray | 2.60 | A | 2-212 | [»] | |
1GNW | X-ray | 2.20 | A/B | 2-212 | [»] | |
5A4U | X-ray | 2.00 | A/B/C/D/E/F | 1-212 | [»] | |
5A4V | X-ray | 2.38 | A/B/C/D/E/F | 1-212 | [»] | |
5A4W | X-ray | 2.25 | A/B/C/D/E/F | 1-212 | [»] | |
5A5K | X-ray | 2.77 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X | 1-212 | [»] | |
SMRi | P46422 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 13222, 8 interactors |
IntActi | P46422, 1 interactor |
STRINGi | 3702.AT4G02520.1 |
PTM databases
iPTMneti | P46422 |
MetOSitei | P46422 |
2D gel databases
SWISS-2DPAGEi | P46422 |
Proteomic databases
PaxDbi | P46422 |
PRIDEi | P46422 |
ProteomicsDBi | 230161 |
Genome annotation databases
EnsemblPlantsi | AT4G02520.1; AT4G02520.1; AT4G02520 |
GeneIDi | 827931 |
Gramenei | AT4G02520.1; AT4G02520.1; AT4G02520 |
KEGGi | ath:AT4G02520 |
Organism-specific databases
Araporti | AT4G02520 |
TAIRi | locus:2132308, AT4G02520 |
Phylogenomic databases
eggNOGi | KOG0867, Eukaryota |
HOGENOMi | CLU_011226_5_1_1 |
InParanoidi | P46422 |
OMAi | DYLAGNN |
OrthoDBi | 1231780at2759 |
PhylomeDBi | P46422 |
Enzyme and pathway databases
BioCyci | ARA:AT4G02520-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P46422 |
PROi | PR:P46422 |
Gene expression databases
ExpressionAtlasi | P46422, baseline and differential |
Genevisiblei | P46422, AT |
Family and domain databases
CDDi | cd03187, GST_C_Phi, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR004045, Glutathione_S-Trfase_N IPR004046, GST_C IPR034347, GST_Phi_C IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF00043, GST_C, 1 hit PF02798, GST_N, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GSTF2_ARATH | |
Accessioni | P46422Primary (citable) accession number: P46422 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 171 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families