UniProtKB - P46413 (GSHB_RAT)
Glutathione synthetase
Gss
Functioni
Catalyzes the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner (PubMed:7862666).
Glutathione (gamma-glutamylcysteinylglycine, GSH) is the most abundant intracellular thiol in living aerobic cells and is required for numerous processes including the protection of cells against oxidative damage, amino acid transport, the detoxification of foreign compounds, the maintenance of protein sulfhydryl groups in a reduced state and acts as a cofactor for a number of enzymes (PubMed:7862666).
1 Publication1 PublicationCatalytic activityi
Cofactori
: glutathione biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.1 Publication This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 125 | SubstrateBy similarity | 1 | |
Metal bindingi | 144 | MagnesiumBy similarity | 1 | |
Binding sitei | 144 | ATPBy similarity | 1 | |
Metal bindingi | 146 | MagnesiumBy similarity | 1 | |
Binding sitei | 220 | SubstrateBy similarity | 1 | |
Binding sitei | 305 | ATPBy similarity | 1 | |
Metal bindingi | 368 | MagnesiumBy similarity | 1 | |
Binding sitei | 375 | ATPBy similarity | 1 | |
Binding sitei | 425 | ATPBy similarity | 1 | |
Binding sitei | 450 | SubstrateBy similarity | 1 | |
Binding sitei | 452 | ATPBy similarity | 1 | |
Binding sitei | 458 | ATP; via carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 364 – 373 | ATPBy similarity | 10 | |
Nucleotide bindingi | 398 – 401 | ATPBy similarity | 4 |
GO - Molecular functioni
- ATP binding Source: RGD
- glutathione binding Source: RGD
- glutathione synthase activity Source: RGD
- glycine binding Source: RGD
- identical protein binding Source: RGD
- magnesium ion binding Source: UniProtKB
- peptide binding Source: RGD
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- aging Source: RGD
- glutathione biosynthetic process Source: RGD
- response to amino acid Source: RGD
- response to cadmium ion Source: RGD
- response to nutrient levels Source: RGD
- response to tumor necrosis factor Source: RGD
- response to xenobiotic stimulus Source: RGD
Keywordsi
Molecular function | Ligase |
Biological process | Glutathione biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.3.2.3, 5301 |
Reactomei | R-RNO-174403, Glutathione synthesis and recycling |
UniPathwayi | UPA00142;UER00210 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutathione synthetase1 Publication (EC:6.3.2.31 Publication)Short name: GSH synthetase Short name: GSH-S Alternative name(s): Glutathione synthase |
Gene namesi | Name:Gss |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2752, Gss |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000211263 | 2 – 474 | Glutathione synthetaseAdd BLAST | 473 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 415 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P46413 |
PaxDbi | P46413 |
PRIDEi | P46413 |
PTM databases
iPTMneti | P46413 |
PhosphoSitePlusi | P46413 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000018964, Expressed in kidney and 22 other tissues |
Genevisiblei | P46413, RN |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000025657 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 148 – 151 | Substrate bindingBy similarity | 4 | |
Regioni | 214 – 216 | Substrate bindingBy similarity | 3 | |
Regioni | 267 – 270 | Substrate bindingBy similarity | 4 | |
Regioni | 461 – 462 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0021, Eukaryota |
GeneTreei | ENSGT00390000013764 |
HOGENOMi | CLU_025152_2_1_1 |
InParanoidi | P46413 |
OMAi | CPTVLTQ |
OrthoDBi | 1189955at2759 |
PhylomeDBi | P46413 |
TreeFami | TF105187 |
Family and domain databases
CDDi | cd00228, eu-GS, 1 hit |
Gene3Di | 1.10.1080.10, 1 hit 3.30.1490.50, 1 hit 3.30.1490.80, 1 hit 3.40.50.1760, 1 hit |
InterProi | View protein in InterPro IPR005615, Glutathione_synthase IPR014042, Glutathione_synthase_a-hlx IPR014709, Glutathione_synthase_C_euk IPR014049, Glutathione_synthase_N_euk IPR037013, GSH-S_sub-bd_sf IPR004887, GSH_synth_subst-bd IPR016185, PreATP-grasp_dom_sf |
PANTHERi | PTHR11130, PTHR11130, 1 hit |
Pfami | View protein in Pfam PF03917, GSH_synth_ATP, 1 hit PF03199, GSH_synthase, 1 hit |
PIRSFi | PIRSF001558, GSHase, 1 hit |
SUPFAMi | SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01986, glut_syn_euk, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MATSWGSILQ DEKQLEELAQ QAIDRALAEG VLLRSAKNPS SSDVVTYAPF
60 70 80 90 100
TLFPSPVPST LLEQAYAVQM DFNILVDAVS QNSAFLEQTL SSTIKKDEYT
110 120 130 140 150
ARLFDIYKQV LKEGIAQTVF LGLNRSDYMF QCSADGSKAL KQIEINTISA
160 170 180 190 200
SFGGLASRTP AVHRHVLNVL NKTNEASKIL SNNPSKGLAL GIAKAWELYG
210 220 230 240 250
SANAVVLLIA QEKERNIFDQ RAIENELLDR KIHVIRRRFE DVSERGSLDQ
260 270 280 290 300
NRRLFMEDQE VAVVYFRDGY MPSQYNAQNW EARLLLERSC AAKCPDIATQ
310 320 330 340 350
LAGTKKVQQE LSRVGLLEAL LPGQPEAVAR LRATFAGLYS LDMGEEGDQA
360 370 380 390 400
VAEALAAPSH FVLKPQREGG GNNFYGEEMV HALEQLKDSE ERASYILMEK
410 420 430 440 450
IEPEPFRNCL LRPGSPAQVV QCISELGIFG VYVRQGTTLV MNKHVGHLLR
460 470
TKAIEHADGG VAAGVAVLDN PYPV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L38615 mRNA Translation: AAA64618.1 BC078700 mRNA Translation: AAH78700.1 |
PIRi | I59346 |
RefSeqi | NP_037094.1, NM_012962.1 |
Genome annotation databases
Ensembli | ENSRNOT00000025657; ENSRNOP00000025657; ENSRNOG00000018964 |
GeneIDi | 25458 |
KEGGi | rno:25458 |
UCSCi | RGD:2752, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L38615 mRNA Translation: AAA64618.1 BC078700 mRNA Translation: AAH78700.1 |
PIRi | I59346 |
RefSeqi | NP_037094.1, NM_012962.1 |
3D structure databases
SMRi | P46413 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000025657 |
PTM databases
iPTMneti | P46413 |
PhosphoSitePlusi | P46413 |
Proteomic databases
jPOSTi | P46413 |
PaxDbi | P46413 |
PRIDEi | P46413 |
Genome annotation databases
Ensembli | ENSRNOT00000025657; ENSRNOP00000025657; ENSRNOG00000018964 |
GeneIDi | 25458 |
KEGGi | rno:25458 |
UCSCi | RGD:2752, rat |
Organism-specific databases
CTDi | 2937 |
RGDi | 2752, Gss |
Phylogenomic databases
eggNOGi | KOG0021, Eukaryota |
GeneTreei | ENSGT00390000013764 |
HOGENOMi | CLU_025152_2_1_1 |
InParanoidi | P46413 |
OMAi | CPTVLTQ |
OrthoDBi | 1189955at2759 |
PhylomeDBi | P46413 |
TreeFami | TF105187 |
Enzyme and pathway databases
UniPathwayi | UPA00142;UER00210 |
BRENDAi | 6.3.2.3, 5301 |
Reactomei | R-RNO-174403, Glutathione synthesis and recycling |
Miscellaneous databases
PROi | PR:P46413 |
Gene expression databases
Bgeei | ENSRNOG00000018964, Expressed in kidney and 22 other tissues |
Genevisiblei | P46413, RN |
Family and domain databases
CDDi | cd00228, eu-GS, 1 hit |
Gene3Di | 1.10.1080.10, 1 hit 3.30.1490.50, 1 hit 3.30.1490.80, 1 hit 3.40.50.1760, 1 hit |
InterProi | View protein in InterPro IPR005615, Glutathione_synthase IPR014042, Glutathione_synthase_a-hlx IPR014709, Glutathione_synthase_C_euk IPR014049, Glutathione_synthase_N_euk IPR037013, GSH-S_sub-bd_sf IPR004887, GSH_synth_subst-bd IPR016185, PreATP-grasp_dom_sf |
PANTHERi | PTHR11130, PTHR11130, 1 hit |
Pfami | View protein in Pfam PF03917, GSH_synth_ATP, 1 hit PF03199, GSH_synthase, 1 hit |
PIRSFi | PIRSF001558, GSHase, 1 hit |
SUPFAMi | SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01986, glut_syn_euk, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GSHB_RAT | |
Accessioni | P46413Primary (citable) accession number: P46413 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families