UniProtKB - P46367 (ALDH4_YEAST)
Protein
Potassium-activated aldehyde dehydrogenase, mitochondrial
Gene
ALD4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Potassium-activated aldehyde dehydrogenase involved in acetate formation during anaerobic growth on glucose.1 Publication
Miscellaneous
Present with 22200 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 192 | Transition state stabilizerBy similarity | 1 | |
Active sitei | 290 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Active sitei | 324 | NucleophilePROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 268 – 273 | NADBy similarity | 6 |
GO - Molecular functioni
- aldehyde dehydrogenase (NAD+) activity Source: SGD
- aldehyde dehydrogenase [NAD(P)+] activity Source: SGD
GO - Biological processi
- acetate biosynthetic process Source: SGD
- ethanol metabolic process Source: SGD
- NADPH regeneration Source: SGD
- pyruvate metabolic process Source: SGD
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD |
Enzyme and pathway databases
Reactomei | R-SCE-196757, Metabolism of folate and pterines R-SCE-5365859, RA biosynthesis pathway R-SCE-70350, Fructose catabolism R-SCE-71384, Ethanol oxidation |
SABIO-RKi | P46367 |
Names & Taxonomyi
Protein namesi | Recommended name: Potassium-activated aldehyde dehydrogenase, mitochondrial (EC:1.2.1.5)Alternative name(s): K(+)-activated acetaldehyde dehydrogenase Short name: K(+)-ACDH |
Gene namesi | Name:ALD4 Synonyms:ALD7, ALDH2 Ordered Locus Names:YOR374W ORF Names:O6730 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005901, ALD4 |
VEuPathDBi | FungiDB:YOR374W |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix 1 Publication
Mitochondrion
- mitochondrial nucleoid Source: SGD
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 24 | Mitochondrion2 PublicationsAdd BLAST | 24 | |
ChainiPRO_0000007165 | 25 – 519 | Potassium-activated aldehyde dehydrogenase, mitochondrialAdd BLAST | 495 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 96 | PhosphoserineCombined sources | 1 | |
Modified residuei | 216 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 500 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P46367 |
PaxDbi | P46367 |
PRIDEi | P46367 |
TopDownProteomicsi | P46367 |
PTM databases
iPTMneti | P46367 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 34757, 69 interactors |
DIPi | DIP-4053N |
IntActi | P46367, 43 interactors |
MINTi | P46367 |
STRINGi | 4932.YOR374W |
Miscellaneous databases
RNActi | P46367, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the aldehyde dehydrogenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2450, Eukaryota |
GeneTreei | ENSGT00940000176713 |
HOGENOMi | CLU_005391_0_2_1 |
InParanoidi | P46367 |
OMAi | NYIVGGL |
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P46367-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFSRSTLCLK TSASSIGRLQ LRYFSHLPMT VPIKLPNGLE YEQPTGLFIN
60 70 80 90 100
NKFVPSKQNK TFEVINPSTE EEICHIYEGR EDDVEEAVQA ADRAFSNGSW
110 120 130 140 150
NGIDPIDRGK ALYRLAELIE QDKDVIASIE TLDNGKAISS SRGDVDLVIN
160 170 180 190 200
YLKSSAGFAD KIDGRMIDTG RTHFSYTKRQ PLGVCGQIIP WNFPLLMWAW
210 220 230 240 250
KIAPALVTGN TVVLKTAEST PLSALYVSKY IPQAGIPPGV INIVSGFGKI
260 270 280 290 300
VGEAITNHPK IKKVAFTGST ATGRHIYQSA AAGLKKVTLE LGGKSPNIVF
310 320 330 340 350
ADAELKKAVQ NIILGIYYNS GEVCCAGSRV YVEESIYDKF IEEFKAASES
360 370 380 390 400
IKVGDPFDES TFQGAQTSQM QLNKILKYVD IGKNEGATLI TGGERLGSKG
410 420 430 440 450
YFIKPTVFGD VKEDMRIVKE EIFGPVVTVT KFKSADEVIN MANDSEYGLA
460 470 480 490 500
AGIHTSNINT ALKVADRVNA GTVWINTYND FHHAVPFGGF NASGLGREMS
510
VDALQNYLQV KAVRAKLDE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 51 | N → NN AA sequence (PubMed:1989592).Curated | 1 | |
Sequence conflicti | 63 | E → V AA sequence (PubMed:1989592).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z75282 Genomic DNA Translation: CAA99705.1 BK006948 Genomic DNA Translation: DAA11133.1 |
PIRi | S67286 |
RefSeqi | NP_015019.1, NM_001183794.1 |
Genome annotation databases
EnsemblFungii | YOR374W_mRNA; YOR374W; YOR374W |
GeneIDi | 854556 |
KEGGi | sce:YOR374W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z75282 Genomic DNA Translation: CAA99705.1 BK006948 Genomic DNA Translation: DAA11133.1 |
PIRi | S67286 |
RefSeqi | NP_015019.1, NM_001183794.1 |
3D structure databases
SMRi | P46367 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34757, 69 interactors |
DIPi | DIP-4053N |
IntActi | P46367, 43 interactors |
MINTi | P46367 |
STRINGi | 4932.YOR374W |
PTM databases
iPTMneti | P46367 |
Proteomic databases
MaxQBi | P46367 |
PaxDbi | P46367 |
PRIDEi | P46367 |
TopDownProteomicsi | P46367 |
Genome annotation databases
EnsemblFungii | YOR374W_mRNA; YOR374W; YOR374W |
GeneIDi | 854556 |
KEGGi | sce:YOR374W |
Organism-specific databases
SGDi | S000005901, ALD4 |
VEuPathDBi | FungiDB:YOR374W |
Phylogenomic databases
eggNOGi | KOG2450, Eukaryota |
GeneTreei | ENSGT00940000176713 |
HOGENOMi | CLU_005391_0_2_1 |
InParanoidi | P46367 |
OMAi | NYIVGGL |
Enzyme and pathway databases
Reactomei | R-SCE-196757, Metabolism of folate and pterines R-SCE-5365859, RA biosynthesis pathway R-SCE-70350, Fructose catabolism R-SCE-71384, Ethanol oxidation |
SABIO-RKi | P46367 |
Miscellaneous databases
PROi | PR:P46367 |
RNActi | P46367, protein |
Family and domain databases
Gene3Di | 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
InterProi | View protein in InterPro IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016160, Ald_DH_CS_CYS IPR029510, Ald_DH_CS_GLU IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom |
Pfami | View protein in Pfam PF00171, Aldedh, 1 hit |
SUPFAMi | SSF53720, SSF53720, 1 hit |
PROSITEi | View protein in PROSITE PS00070, ALDEHYDE_DEHYDR_CYS, 1 hit PS00687, ALDEHYDE_DEHYDR_GLU, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ALDH4_YEAST | |
Accessioni | P46367Primary (citable) accession number: P46367 Secondary accession number(s): D6W367, Q08898 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 183 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - SIMILARITY comments
Index of protein domains and families - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names