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UniProtKB - P46344 (PGPH_BACSU)
Protein
Cyclic-di-AMP phosphodiesterase PgpH
Gene
pgpH
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Probably has phosphodiesterase (PDE) activity against cyclic-di-AMP (c-di-AMP); may be the major c-di-AMP PDE in the cell (PubMed:26240071).
In B.subtilis c-di-AMP is a second messenger that mediates growth, DNA repair and cell wall homeostasis; it is toxic when present in excess (PubMed:26240071).
1 PublicationCatalytic activityi
- EC:3.1.4.59By similarity
Cofactori
Mn2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 504 | Manganese 1; via tele nitrogenBy similarity | 1 | |
Binding sitei | 504 | SubstrateBy similarity | 1 | |
Metal bindingi | 533 | Manganese 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 534 | Manganese 1By similarity | 1 | |
Metal bindingi | 534 | Manganese 2By similarity | 1 | |
Metal bindingi | 570 | Manganese 2; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 594 | Manganese 2; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 595 | Manganese 2; via tele nitrogenBy similarity | 1 | |
Binding sitei | 621 | SubstrateBy similarity | 1 | |
Metal bindingi | 638 | Manganese 1By similarity | 1 | |
Binding sitei | 638 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- cyclic-di-AMP phosphodiesterase activity Source: UniProtKB-EC
- hydrolase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU25330-MONOMER |
BRENDAi | 3.1.4.59, 658 |
Names & Taxonomyi
Protein namesi | Recommended name: Cyclic-di-AMP phosphodiesterase PgpH1 Publication (EC:3.1.4.59By similarity)Short name: c-di-AMP phosphodiesterase |
Gene namesi | Name:pgpH1 Publication Synonyms:yqfF Ordered Locus Names:BSU25330 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein Curated
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 275 – 295 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 311 – 331 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 336 – 356 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 360 – 380 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 384 – 404 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 413 – 433 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 448 – 468 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
In strain 168 grown in minimal medium and glutamate, 3.5-fold increase in c-di-AMP levels, while a double pgpH-gdpP mutant has 4.2-fold increased levels of in c-di-AMP; double mutants die on solid medium after 2 days (PubMed:26240071).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000049797 | 1 – 711 | Cyclic-di-AMP phosphodiesterase PgpHAdd BLAST | 711 |
Proteomic databases
jPOSTi | P46344 |
PaxDbi | P46344 |
Interactioni
Protein-protein interaction databases
IntActi | P46344, 38 interactors |
STRINGi | 224308.BSU25330 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 501 – 643 | HDPROSITE-ProRule annotationAdd BLAST | 143 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 114 – 133 | DisorderedSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Belongs to the PgpH phosphodiesterase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1480, Bacteria |
InParanoidi | P46344 |
OMAi | DFIRTHH |
PhylomeDBi | P46344 |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
InterProi | View protein in InterPro IPR003607, HD/PDEase_dom IPR006674, HD_domain IPR006675, HDIG_dom IPR011624, Metal-dep_PHydrolase_7TM_extra IPR011621, Metal-dep_PHydrolase_7TM_intra |
Pfami | View protein in Pfam PF07698, 7TM-7TMR_HD, 1 hit PF07697, 7TMR-HDED, 1 hit PF01966, HD, 1 hit |
SMARTi | View protein in SMART SM00471, HDc, 1 hit |
TIGRFAMsi | TIGR00277, HDIG, 1 hit |
PROSITEi | View protein in PROSITE PS51831, HD, 1 hit |
i Sequence
Sequence statusi: Complete.
P46344-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLKKKAKTKS SSKKWSSFKN ARSMHVLLYL LLAAIMFALL FVHVKPETLD
60 70 80 90 100
LDLFSVSDKT IYAPATVEDQ KATEEKKQAA EDAVEDQYTL KKEYTDNRID
110 120 130 140 150
LVSSIFDSIS EVKKSSEEGS KSPSEKSMVK SVKDKLTSDV NDSISEDSIK
160 170 180 190 200
TLLKADSEDF SFVRDTVITA VNTVMSSEIP SDKLSDAKDK VEKELKSNSI
210 220 230 240 250
PSKYLGAATE IGRFAIIPNY VFDPKATEAK RQEASDNVQQ VQIKQGQVLV
260 270 280 290 300
EENDLIDREV YRKLELTGLL NNSNLFKPIS GLLIMIGLFI ATLVYYFEKQ
310 320 330 340 350
KQNLKFKNQS ILLFSIITTL LLVIMEVVSL FQKMEYNNIG YLVPIAAGAI
360 370 380 390 400
LIKLLMNERI AILGSIILAI CGSMMFNQGV TGTFNYVIGI YYLISGISGV
410 420 430 440 450
LFLGKHNARS KILQTGLFVA FINMVVVLSL LLIQNTALSG LEIGTLMLMG
460 470 480 490 500
VVSGFASSVL IIGLMPFFET GFGILSTMRL LELSNPNHPL LRKILTETPG
510 520 530 540 550
TYHHSVMVAN LSEAACEAVG ANGLLARVGA YYHDLGKTKR PQYFIENQMN
560 570 580 590 600
IDNPHDKLSP QLSKNIIISH TTDGANMLRS YKFPKELVDI AEQHHGTSLL
610 620 630 640 650
KFFYYKAKEK GDQITEEEFR YPGPKPQSKE AAIISVADSV EAAVRSMHNP
660 670 680 690 700
NPERIEKLVR GIISDKLQDG QFSECDLTFK ELDTIAKTLC ATLKGIFHSR
710
IEYPEATKKV K
Sequence cautioni
The sequence AAA70040 differs from that shown. Reason: Frameshift.Curated
The sequence AAA70041 differs from that shown. Reason: Frameshift.Curated
The sequence AAA70042 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84432 Genomic DNA Translation: BAA12478.1 U29177 Genomic DNA Translation: AAA70040.1 Frameshift. U29177 Genomic DNA Translation: AAA70041.1 Frameshift. U29177 Genomic DNA Translation: AAA70042.1 Frameshift. AL009126 Genomic DNA Translation: CAB14462.1 |
PIRi | E69953 |
RefSeqi | NP_390411.1, NC_000964.3 WP_010886570.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14462; CAB14462; BSU_25330 |
GeneIDi | 937877 |
KEGGi | bsu:BSU25330 |
PATRICi | fig|224308.43.peg.2641 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84432 Genomic DNA Translation: BAA12478.1 U29177 Genomic DNA Translation: AAA70040.1 Frameshift. U29177 Genomic DNA Translation: AAA70041.1 Frameshift. U29177 Genomic DNA Translation: AAA70042.1 Frameshift. AL009126 Genomic DNA Translation: CAB14462.1 |
PIRi | E69953 |
RefSeqi | NP_390411.1, NC_000964.3 WP_010886570.1, NZ_JNCM01000036.1 |
3D structure databases
AlphaFoldDBi | P46344 |
SMRi | P46344 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P46344, 38 interactors |
STRINGi | 224308.BSU25330 |
Proteomic databases
jPOSTi | P46344 |
PaxDbi | P46344 |
Genome annotation databases
EnsemblBacteriai | CAB14462; CAB14462; BSU_25330 |
GeneIDi | 937877 |
KEGGi | bsu:BSU25330 |
PATRICi | fig|224308.43.peg.2641 |
Phylogenomic databases
eggNOGi | COG1480, Bacteria |
InParanoidi | P46344 |
OMAi | DFIRTHH |
PhylomeDBi | P46344 |
Enzyme and pathway databases
BioCyci | BSUB:BSU25330-MONOMER |
BRENDAi | 3.1.4.59, 658 |
Family and domain databases
CDDi | cd00077, HDc, 1 hit |
InterProi | View protein in InterPro IPR003607, HD/PDEase_dom IPR006674, HD_domain IPR006675, HDIG_dom IPR011624, Metal-dep_PHydrolase_7TM_extra IPR011621, Metal-dep_PHydrolase_7TM_intra |
Pfami | View protein in Pfam PF07698, 7TM-7TMR_HD, 1 hit PF07697, 7TMR-HDED, 1 hit PF01966, HD, 1 hit |
SMARTi | View protein in SMART SM00471, HDc, 1 hit |
TIGRFAMsi | TIGR00277, HDIG, 1 hit |
PROSITEi | View protein in PROSITE PS51831, HD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PGPH_BACSU | |
Accessioni | P46344Primary (citable) accession number: P46344 Secondary accession number(s): P46345, P46346 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 138 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families