UniProtKB - P46320 (LICH_BACSU)
Protein
Probable 6-phospho-beta-glucosidase
Gene
licH
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Hydrolyzes phospho-beta-glucosides.Curated
Catalytic activityi
- EC:3.2.1.86
Cofactori
Protein has several cofactor binding sites:- NAD+By similarity
- a divalent metal cationBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 96 | SubstrateBy similarity | 1 | |
Sitei | 112 | Increases basicity of active site TyrBy similarity | 1 | |
Binding sitei | 150 | SubstrateBy similarity | 1 | |
Metal bindingi | 172 | ManganeseBy similarity | 1 | |
Metal bindingi | 202 | ManganeseBy similarity | 1 | |
Active sitei | 256 | Proton acceptorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 5 – 73 | NADBy similarityAdd BLAST | 69 |
GO - Molecular functioni
- 6-phospho-beta-glucosidase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- methyl beta-D-glucoside 6-phosphate glucohydrolase activity Source: UniProtKB-EC
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism |
Ligand | Manganese, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | BSUB:BSU38560-MONOMER |
Protein family/group databases
CAZyi | GH4, Glycoside Hydrolase Family 4 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:licH Synonyms:celD, celF Ordered Locus Names:BSU38560 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169861 | 1 – 442 | Probable 6-phospho-beta-glucosidaseAdd BLAST | 442 |
Proteomic databases
jPOSTi | P46320 |
PaxDbi | P46320 |
PRIDEi | P46320 |
Expressioni
Inductioni
Induced by lichenan, lichenan hydrolysate and cellobiose. Subject to carbon catabolite repression.
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 4 family.Curated
Phylogenomic databases
eggNOGi | COG1486, Bacteria |
InParanoidi | P46320 |
OMAi | NEHASHI |
PhylomeDBi | P46320 |
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
InterProi | View protein in InterPro IPR019802, GlycHydrolase_4_CS IPR001088, Glyco_hydro_4 IPR022616, Glyco_hydro_4_C IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR32092, PTHR32092, 1 hit |
Pfami | View protein in Pfam PF02056, Glyco_hydro_4, 1 hit PF11975, Glyco_hydro_4C, 1 hit |
PRINTSi | PR00732, GLHYDRLASE4 |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS01324, GLYCOSYL_HYDROL_F4, 1 hit |
i Sequence
Sequence statusi: Complete.
P46320-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKGLKIVTI GGGSSYTPEL VEGFIKRYDE LPVRELWLVD IPEGEEKLNI
60 70 80 90 100
VGTLAKRMVE KAGVPIDIHL TLDRRKALKD ADFVTTQFRV GLLQARAKDE
110 120 130 140 150
RIPLKYGVIG QETNGPGGLF KGLRTIPVIL EIAKDIEELC PNAWLVNFTN
160 170 180 190 200
PAGMVTEALL RYSNLKKVVG LCNVPIGIKM GVAKALDVDV DRVEVQFAGL
210 220 230 240 250
NHMVFGLDVF LDGVSVKEQV IEAMGDPKNA MTMKNISGAE WEPDFLKALN
260 270 280 290 300
VIPCGYHRYY FKTKEMLEHE LEASQTEGTR AEVVQKVEKE LFELYKDPNL
310 320 330 340 350
AIKPPQLEKR GGAYYSDAAC NLISSIYNDK HDIQPVNTIN NGAIASIPDD
360 370 380 390 400
SAVEVNCVMT KTGPKPIAVG DLPVSVRGLV QQIKSFERVA AEAAVTGDYQ
410 420 430 440
TALLAMTINP LVPSDTVAKQ ILDEMLEAHK AYLPQFFNKI EA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49992 Genomic DNA Translation: CAA90288.1 AL009126 Genomic DNA Translation: CAB15882.1 D83026 Genomic DNA Translation: BAA11746.1 |
PIRi | S57762 |
RefSeqi | NP_391735.1, NC_000964.3 WP_003244285.1, NZ_JNCM01000034.1 |
Genome annotation databases
EnsemblBacteriai | CAB15882; CAB15882; BSU_38560 |
GeneIDi | 937373 |
KEGGi | bsu:BSU38560 |
PATRICi | fig|224308.179.peg.4175 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49992 Genomic DNA Translation: CAA90288.1 AL009126 Genomic DNA Translation: CAB15882.1 D83026 Genomic DNA Translation: BAA11746.1 |
PIRi | S57762 |
RefSeqi | NP_391735.1, NC_000964.3 WP_003244285.1, NZ_JNCM01000034.1 |
3D structure databases
SMRi | P46320 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU38560 |
Protein family/group databases
CAZyi | GH4, Glycoside Hydrolase Family 4 |
Proteomic databases
jPOSTi | P46320 |
PaxDbi | P46320 |
PRIDEi | P46320 |
Genome annotation databases
EnsemblBacteriai | CAB15882; CAB15882; BSU_38560 |
GeneIDi | 937373 |
KEGGi | bsu:BSU38560 |
PATRICi | fig|224308.179.peg.4175 |
Phylogenomic databases
eggNOGi | COG1486, Bacteria |
InParanoidi | P46320 |
OMAi | NEHASHI |
PhylomeDBi | P46320 |
Enzyme and pathway databases
BioCyci | BSUB:BSU38560-MONOMER |
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
InterProi | View protein in InterPro IPR019802, GlycHydrolase_4_CS IPR001088, Glyco_hydro_4 IPR022616, Glyco_hydro_4_C IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR32092, PTHR32092, 1 hit |
Pfami | View protein in Pfam PF02056, Glyco_hydro_4, 1 hit PF11975, Glyco_hydro_4C, 1 hit |
PRINTSi | PR00732, GLHYDRLASE4 |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS01324, GLYCOSYL_HYDROL_F4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LICH_BACSU | |
Accessioni | P46320Primary (citable) accession number: P46320 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families