UniProtKB - P46133 (ABGT_ECOLI)
Protein
p-aminobenzoyl-glutamate transport protein
Gene
abgT
Organism
Escherichia coli (strain K12)
Status
Functioni
Essential for aminobenzoyl-glutamate utilization. It catalyzes the concentration-dependent uptake of p-aminobenzoyl-glutamate (PABA-GLU) into the cell and allows accumulation of PABA-GLU to a concentration enabling AbgAB to catalyze cleavage into p-aminobenzoate and glutamate. It seems also to increase the sensitivity to low levels of aminobenzoyl-glutamate. May actually serve physiologically as a transporter for some other molecule, perhaps a dipeptide, and that it transports p-aminobenzoyl-glutamate as a secondary activity. The physiological role of abgABT should be clarified.2 Publications
Miscellaneous
It is suggested that in wild-type strain abgT would normally be cryptic or not expressed under conditions of growth on minimal medium. Altering the expression of abgT from its wild-type context appears to be the mechanism for obtaining the growth phenotype (PubMed:9829935).1 Publication
Activity regulationi
Completely inhibited by 100 nM of sodium azide and slightly inhibited by 100 mM of potassium fluoride.1 Publication
GO - Molecular functioni
- secondary active p-aminobenzoyl-glutamate transmembrane transporter activity Source: EcoCyc
- secondary active transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- amino acid transport Source: UniProtKB-KW
- p-aminobenzoyl-glutamate transmembrane transport Source: InterPro
- p-aminobenzoyl-glutamate transport Source: EcoCyc
Keywordsi
Biological process | Amino-acid transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:ABGT-MONOMER MetaCyc:ABGT-MONOMER |
Protein family/group databases
TCDBi | 2.A.68.1.1, the p-aminobenzoyl-glutamate transporter (abgt) family |
Names & Taxonomyi
Protein namesi | Recommended name: p-aminobenzoyl-glutamate transport proteinAlternative name(s): PABA-GLU transport protein |
Gene namesi | Name:abgT Synonyms:ydaH Ordered Locus Names:b1336, JW5822 ORF Names:ECK1332 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 30 – 50 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 85 – 105 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 121 – 139 | HelicalSequence analysisAdd BLAST | 19 | |
Transmembranei | 140 – 159 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 164 – 184 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 211 – 231 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 261 – 281 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 303 – 323 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 343 – 363 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 382 – 402 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 405 – 425 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 439 – 459 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 479 – 499 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cells lacking this gene loss the p-aminobenzoyl-glutamate utilization phenotype.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064422 | 1 – 508 | p-aminobenzoyl-glutamate transport proteinAdd BLAST | 508 |
Proteomic databases
PaxDbi | P46133 |
PRIDEi | P46133 |
Expressioni
Inductioni
Could be transcriptionally regulated by AbgR.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260156, 187 interactors |
DIPi | DIP-9031N |
IntActi | P46133, 1 interactor |
STRINGi | 511145.b1336 |
Family & Domainsi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2978, Bacteria |
HOGENOMi | CLU_040132_0_0_6 |
InParanoidi | P46133 |
PhylomeDBi | P46133 |
Family and domain databases
InterProi | View protein in InterPro IPR004697, AbgT IPR011540, AbgT_Proteobac |
PANTHERi | PTHR30282, PTHR30282, 1 hit |
Pfami | View protein in Pfam PF03806, ABG_transport, 1 hit |
TIGRFAMsi | TIGR00819, ydaH, 1 hit |
i Sequence
Sequence statusi: Complete.
P46133-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSMSSIPSSS QSGKLYGWVE RIGNKVPHPF LLFIYLIIVL MVTTAILSAF
60 70 80 90 100
GVSAKNPTDG TPVVVKNLLS VEGLHWFLPN VIKNFSGFAP LGAILALVLG
110 120 130 140 150
AGLAERVGLL PALMVKMASH VNARYASYMV LFIAFFSHIS SDAALVIMPP
160 170 180 190 200
MGALIFLAVG RHPVAGLLAA IAGVGCGFTA NLLIVTTDVL LSGISTEAAA
210 220 230 240 250
AFNPQMHVSV IDNWYFMASS VVVLTIVGGL ITDKIIEPRL GQWQGNSDEK
260 270 280 290 300
LQTLTESQRF GLRIAGVVSL LFIAAIALMV IPQNGILRDP INHTVMPSPF
310 320 330 340 350
IKGIVPLIIL FFFVVSLAYG IATRTIRRQA DLPHLMIEPM KEMAGFIVMV
360 370 380 390 400
FPLAQFVAMF NWSNMGKFIA VGLTDILESS GLSGIPAFVG LALLSSFLCM
410 420 430 440 450
FIASGSAIWS ILAPIFVPMF MLLGFHPAFA QILFRIADSS VLPLAPVSPF
460 470 480 490 500
VPLFLGFLQR YKPDAKLGTY YSLVLPYPLI FLVVWLLMLL AWYLVGLPIG
PGIYPRLS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 504 | Y → S in BAA14929 (PubMed:9097039).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74418.2 AP009048 Genomic DNA Translation: BAA14929.2 Y00495 Genomic DNA No translation available. |
PIRi | C64883 |
RefSeqi | NP_415852.2, NC_000913.3 WP_000062973.1, NZ_SSUW01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC74418; AAC74418; b1336 BAA14929; BAA14929; BAA14929 |
GeneIDi | 945912 |
KEGGi | ecj:JW5822 eco:b1336 |
PATRICi | fig|1411691.4.peg.941 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74418.2 AP009048 Genomic DNA Translation: BAA14929.2 Y00495 Genomic DNA No translation available. |
PIRi | C64883 |
RefSeqi | NP_415852.2, NC_000913.3 WP_000062973.1, NZ_SSUW01000011.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260156, 187 interactors |
DIPi | DIP-9031N |
IntActi | P46133, 1 interactor |
STRINGi | 511145.b1336 |
Protein family/group databases
TCDBi | 2.A.68.1.1, the p-aminobenzoyl-glutamate transporter (abgt) family |
Proteomic databases
PaxDbi | P46133 |
PRIDEi | P46133 |
Protocols and materials databases
DNASUi | 945912 |
Genome annotation databases
EnsemblBacteriai | AAC74418; AAC74418; b1336 BAA14929; BAA14929; BAA14929 |
GeneIDi | 945912 |
KEGGi | ecj:JW5822 eco:b1336 |
PATRICi | fig|1411691.4.peg.941 |
Organism-specific databases
EchoBASEi | EB2697 |
Phylogenomic databases
eggNOGi | COG2978, Bacteria |
HOGENOMi | CLU_040132_0_0_6 |
InParanoidi | P46133 |
PhylomeDBi | P46133 |
Enzyme and pathway databases
BioCyci | EcoCyc:ABGT-MONOMER MetaCyc:ABGT-MONOMER |
Miscellaneous databases
PROi | PR:P46133 |
Family and domain databases
InterProi | View protein in InterPro IPR004697, AbgT IPR011540, AbgT_Proteobac |
PANTHERi | PTHR30282, PTHR30282, 1 hit |
Pfami | View protein in Pfam PF03806, ABG_transport, 1 hit |
TIGRFAMsi | TIGR00819, ydaH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ABGT_ECOLI | |
Accessioni | P46133Primary (citable) accession number: P46133 Secondary accession number(s): P76051, P76843, P76845 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | January 15, 2008 | |
Last modified: | December 2, 2020 | |
This is version 152 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |