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Protein

Putative acyl-CoA thioester hydrolase YbhC

Gene

ybhC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Putative thioesterase. Does not bind pectin, and has no pectinesterase activity.2 Publications

Caution

Lacks the conserved Asp residue in position 252 essential for pectinase activity. Likewise, most of the residues involved in substrate binding are not conserved. Was originally (PubMed:15808744) thought to have palmitoyl-CoA thioesterase activity, but PubMed:19452549 were unable to detect any pectinase or palmitoyl-CoA thioesterase activity. Its enzyme activity is therefore unsure.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei285NucleophilePROSITE-ProRule annotation1
Binding sitei345SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl esterase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG12875-MONOMER
BRENDAi3.1.2.2 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Putative acyl-CoA thioester hydrolase YbhC (EC:3.1.2.-)
Gene namesi
Name:ybhC
Ordered Locus Names:b0772, JW0755
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12875 ybhC

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • cell wall Source: InterPro

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21By similarityAdd BLAST21
ChainiPRO_000002350222 – 427Putative acyl-CoA thioester hydrolase YbhCAdd BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi22N-palmitoyl cysteineCurated1
Lipidationi22S-diacylglycerol cysteineCurated1
Disulfide bondi185 ↔ 1971 Publication

Keywords - PTMi

Disulfide bond, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP46130
PRIDEiP46130

Interactioni

Protein-protein interaction databases

BioGridi4261839, 24 interactors
DIPiDIP-11407N
IntActiP46130, 3 interactors
STRINGi316385.ECDH10B_0840

Structurei

Secondary structure

1427
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP46130
SMRiP46130
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46130

Family & Domainsi

Sequence similaritiesi

Belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105NBR Bacteria
COG4677 LUCA
HOGENOMiHOG000118057
InParanoidiP46130
KOiK01051
OMAiFNRMWEY
PhylomeDBiP46130

Family and domain databases

Gene3Di2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
SUPFAMiSSF51126 SSF51126, 3 hits
PROSITEiView protein in PROSITE
PS00800 PECTINESTERASE_1, 1 hit
PS00503 PECTINESTERASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46130-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNTFSVSRLA LALAFGVTLT ACSSTPPDQR PSDQTAPGTS SRPILSAKEA
60 70 80 90 100
QNFDAQHYFA SLTPGAAAWN PSPITLPAQP DFVVGPAGTQ GVTHTTIQAA
110 120 130 140 150
VDAAIIKRTN KRQYIAVMPG EYQGTVYVPA APGGITLYGT GEKPIDVKIG
160 170 180 190 200
LSLDGGMSPA DWRHDVNPRG KYMPGKPAWY MYDSCQSKRS DSIGVLCSAV
210 220 230 240 250
FWSQNNGLQL QNLTIENTLG DSVDAGNHPA VALRTDGDQV QINNVNILGR
260 270 280 290 300
QNTFFVTNSG VQNRLETNRQ PRTLVTNSYI EGDVDIVSGR GAVVFDNTEF
310 320 330 340 350
RVVNSRTQQE AYVFAPATLS NIYYGFLAVN SRFNAFGDGV AQLGRSLDVD
360 370 380 390 400
ANTNGQVVIR DSAINEGFNT AKPWADAVIS NRPFAGNTGS VDDNDEIQRN
410 420
LNDTNYNRMW EYNNRGVGSK VVAEAKK
Length:427
Mass (Da):46,082
Last modified:November 1, 1997 - v2
Checksum:i208831BE0EDBCB5C
GO

Sequence cautioni

The sequence U39938 differs from that shown. Reason: Frameshift at position 37.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167N → Q (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC73859.1
AP009048 Genomic DNA Translation: BAA35436.2
U39938 Genomic DNA No translation available.
J01638 Genomic DNA No translation available.
PIRiD64813
RefSeqiNP_415293.1, NC_000913.3
WP_001091569.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73859; AAC73859; b0772
BAA35436; BAA35436; BAA35436
GeneIDi945381
KEGGiecj:JW0755
eco:b0772
PATRICifig|1411691.4.peg.1506

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC73859.1
AP009048 Genomic DNA Translation: BAA35436.2
U39938 Genomic DNA No translation available.
J01638 Genomic DNA No translation available.
PIRiD64813
RefSeqiNP_415293.1, NC_000913.3
WP_001091569.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GRHX-ray1.70A29-427[»]
ProteinModelPortaliP46130
SMRiP46130
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261839, 24 interactors
DIPiDIP-11407N
IntActiP46130, 3 interactors
STRINGi316385.ECDH10B_0840

Proteomic databases

PaxDbiP46130
PRIDEiP46130

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73859; AAC73859; b0772
BAA35436; BAA35436; BAA35436
GeneIDi945381
KEGGiecj:JW0755
eco:b0772
PATRICifig|1411691.4.peg.1506

Organism-specific databases

EchoBASEiEB2713
EcoGeneiEG12875 ybhC

Phylogenomic databases

eggNOGiENOG4105NBR Bacteria
COG4677 LUCA
HOGENOMiHOG000118057
InParanoidiP46130
KOiK01051
OMAiFNRMWEY
PhylomeDBiP46130

Enzyme and pathway databases

BioCyciEcoCyc:EG12875-MONOMER
BRENDAi3.1.2.2 2026

Miscellaneous databases

EvolutionaryTraceiP46130
PROiPR:P46130

Family and domain databases

Gene3Di2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
SUPFAMiSSF51126 SSF51126, 3 hits
PROSITEiView protein in PROSITE
PS00800 PECTINESTERASE_1, 1 hit
PS00503 PECTINESTERASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiYBHC_ECOLI
AccessioniPrimary (citable) accession number: P46130
Secondary accession number(s): P75765
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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