UniProtKB - P46099 (KLF1_MOUSE)
Krueppel-like factor 1
Klf1
Functioni
Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. Probably serves as a general switch factor for erythroid development. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.
4 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 275 – 299 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 305 – 329 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 335 – 357 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- cis-regulatory region sequence-specific DNA binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription factor binding Source: BHF-UCL
- metal ion binding Source: UniProtKB-KW
- protein domain specific binding Source: BHF-UCL
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- transcription cis-regulatory region binding Source: MGI
GO - Biological processi
- chromatin remodeling Source: MGI
- embryonic hemopoiesis Source: MGI
- erythrocyte development Source: MGI
- erythrocyte differentiation Source: MGI
- erythrocyte maturation Source: MGI
- in utero embryonic development Source: MGI
- liver development Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of transcription, DNA-templated Source: BHF-UCL
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Krueppel-like factor 1Alternative name(s): Erythroid krueppel-like transcription factor Short name: EKLF |
Gene namesi | Name:Klf1 Synonyms:Elkf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1342771, Klf1 |
Subcellular locationi
Nucleus
Note: Colocalizes with SUMO1 in nuclear speckles.
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- chromatin Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 23 | T → A: Greatly reduced transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 23 | T → D: No change in transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 25 – 26 | ED → AG: Abolishes most of transactivation activity. 1 Publication | 2 | |
Mutagenesisi | 56 | K → R: Abolishes sumoylation. No change in nuclear localization but no colocalization with SUMO1. Reduces inhibition of megakaryocytic differentiation. No decrease in bone marrow precursors to form megakaryocytic colonies. No effect on erythroid differentiation. 1 Publication | 1 | |
Mutagenesisi | 270 | K → A or R: No effect on DNA binding. Transactivation activity reduced by 50%. Loss of superactivation by CBP of EP300. 1 Publication | 1 | |
Mutagenesisi | 284 | K → A or R: No effect on DNA binding. No change in transactivation activity. No loss of superactivation by CBP or EP300. 2 Publications | 1 | |
Mutagenesisi | 295 | H → N: Loss of DNA binding and transactivation activity. No change in interaction with SIN3A nor with HDAC1. No effect on repressive activity. 1 Publication | 1 | |
Mutagenesisi | 325 | H → N: Loss of DNA binding and transactivation activity. No change in interaction with SIN3A nor with HDAC1. No effect on repressive activity. 1 Publication | 1 | |
Mutagenesisi | 353 | H → N: Loss of DNA binding and transactivation activity. No change in interaction with SIN3A nor with HDAC1. No effect on repressive activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047161 | 1 – 358 | Krueppel-like factor 1Add BLAST | 358 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 23 | Phosphothreonine; by CK21 Publication | 1 | |
Cross-linki | 56 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) | ||
Modified residuei | 178 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 270 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 284 | N6-acetyllysine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P46099 |
PaxDbi | P46099 |
PRIDEi | P46099 |
ProteomicsDBi | 269222 |
PTM databases
iPTMneti | P46099 |
PhosphoSitePlusi | P46099 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Interacts with CBP and EP300; the interactions enhance the transactivation activity.
Interacts with PCAF; the interaction does not acetylate EKLF and inhibits its transactivation activity (By similarity).
Interacts with CDH4; the interaction is SUMO-dependent and leads to transcriptional repression.
By similarity2 PublicationsBinary interactionsi
P46099
With | #Exp. | IntAct |
---|---|---|
TAF9 [Q16594] from Homo sapiens. | 3 | EBI-15761537,EBI-712521 |
GO - Molecular functioni
- DNA-binding transcription factor binding Source: BHF-UCL
- protein domain specific binding Source: BHF-UCL
Protein-protein interaction databases
DIPi | DIP-48738N |
IntActi | P46099, 2 interactors |
STRINGi | 10090.ENSMUSP00000064366 |
Miscellaneous databases
RNActi | P46099, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 35 – 69 | DisorderedSequence analysisAdd BLAST | 35 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 71 – 79 | 9aaTADBy similarity | 9 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 275 – 299 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 305 – 329 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 335 – 357 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
InParanoidi | P46099 |
PhylomeDBi | P46099 |
Family and domain databases
InterProi | View protein in InterPro IPR031786, EKLF_TAD1 IPR031784, EKLF_TAD2 IPR031785, KLF1 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR23235:SF107, PTHR23235:SF107, 1 hit |
Pfami | View protein in Pfam PF16832, EKLF_TAD1, 1 hit PF16833, EKLF_TAD2, 1 hit PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MASAETVLPS ISTLTTLGQF LDTQEDFLKW WRSEETQDLG PGPPNPTGPS
60 70 80 90 100
LHVSLKSEDP SGEDDERDVT CAWDPDLFLT NFPGSESPGT SRTCALAPSV
110 120 130 140 150
GPVAQFEPPE SLGAYAGGPG LVTGPLGSEE HTSWAHPTPR PPAPEPFVAP
160 170 180 190 200
ALAPGLAPKA QPSYSDSRAG SVGGFFPRAG LAVPAAPGAP YGLLSGYPAL
210 220 230 240 250
YPAPQYQGHF QLFRGLAAPS AGGTAPPSFL NCLGPGTVAT ELGATAIAGD
260 270 280 290 300
AGLSPGTAPP KRSRRTLAPK RQAAHTCGHE GCGKSYSKSS HLKAHLRTHT
310 320 330 340 350
GEKPYACSWD GCDWRFARSD ELTRHYRKHT GHRPFCCGLC PRAFSRSDHL
ALHMKRHL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A6I8MWY6 | A0A6I8MWY6_MOUSE | Krueppel-like factor 1 | Klf1 | 376 | Annotation score: | ||
E9QNP6 | E9QNP6_MOUSE | Krueppel-like factor 1 | Klf1 | 358 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 94 | C → Y in AAC24497 (PubMed:7565748).Curated | 1 | |
Sequence conflicti | 116 | A → T in AAC24497 (PubMed:7565748).Curated | 1 | |
Sequence conflicti | 223 | G → P in AAC24497 (PubMed:7565748).Curated | 1 | |
Sequence conflicti | 287 | S → T in AAC24497 (PubMed:7565748).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M97200 mRNA Translation: AAA37546.1 Different initiation. AF019074 Genomic DNA Translation: AAB87861.1 AF033102 Genomic DNA Translation: AAC24497.1 |
CCDSi | CCDS22483.1 |
PIRi | A48060 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M97200 mRNA Translation: AAA37546.1 Different initiation. AF019074 Genomic DNA Translation: AAB87861.1 AF033102 Genomic DNA Translation: AAC24497.1 |
CCDSi | CCDS22483.1 |
PIRi | A48060 |
3D structure databases
SMRi | P46099 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-48738N |
IntActi | P46099, 2 interactors |
STRINGi | 10090.ENSMUSP00000064366 |
PTM databases
iPTMneti | P46099 |
PhosphoSitePlusi | P46099 |
Proteomic databases
MaxQBi | P46099 |
PaxDbi | P46099 |
PRIDEi | P46099 |
ProteomicsDBi | 269222 |
Organism-specific databases
MGIi | MGI:1342771, Klf1 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
InParanoidi | P46099 |
PhylomeDBi | P46099 |
Miscellaneous databases
PROi | PR:P46099 |
RNActi | P46099, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR031786, EKLF_TAD1 IPR031784, EKLF_TAD2 IPR031785, KLF1 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR23235:SF107, PTHR23235:SF107, 1 hit |
Pfami | View protein in Pfam PF16832, EKLF_TAD1, 1 hit PF16833, EKLF_TAD2, 1 hit PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | KLF1_MOUSE | |
Accessioni | P46099Primary (citable) accession number: P46099 Secondary accession number(s): O70261 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1997 | |
Last modified: | September 29, 2021 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families