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Protein

Synaptotagmin-2

Gene

Syt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exhibits calcium-dependent phospholipid and inositol polyphosphate binding properties (PubMed:7961887). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (PubMed:7961887). Plays a role in dendrite formation by melanocytes (By similarity).By similarity1 Publication
(Microbial infection) Receptor for C.botulinum neurotoxin type B (BoNT/B, botB); interaction is improved in the presence of gangliosides (PubMed:14504267). The toxin binds via the vesicular domain (residues 47-60) (PubMed:14504267, PubMed:17167418, PubMed:23807078).3 Publications
(Microbial infection) Receptor for C.botulinum neurotoxin type G (BoNT/G, botG); gangliosides are not required for (or only very slightly improve) binding to a membrane-anchored receptor fragment (PubMed:20219474). The toxin binds via the vesicular domain (residues 47-55) (PubMed:20219474).1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi172Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi173Calcium 1By similarity1
Metal bindingi173Calcium 2By similarity1
Metal bindingi179Calcium 1By similarity1
Metal bindingi231Calcium 1By similarity1
Metal bindingi231Calcium 2By similarity1
Metal bindingi232Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi233Calcium 1By similarity1
Metal bindingi233Calcium 2By similarity1
Metal bindingi233Calcium 3By similarity1
Metal bindingi236Calcium 3By similarity1
Metal bindingi237Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi239Calcium 2By similarity1
Metal bindingi239Calcium 3By similarity1

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: UniProtKB
  • calcium ion binding Source: MGI
  • clathrin binding Source: GO_Central
  • inositol 1,3,4,5 tetrakisphosphate binding Source: UniProtKB
  • phosphatidylserine binding Source: ParkinsonsUK-UCL
  • SNARE binding Source: GO_Central
  • syntaxin binding Source: MGI

GO - Biological processi

Keywordsi

Biological processDifferentiation
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-2Curated
Alternative name(s):
Inositol polyphosphate-binding protein1 Publication
Short name:
IP4-binding protein1 Publication
Short name:
IP4BP1 Publication
Synaptotagmin IIImported
Short name:
SytIIBy similarity
Gene namesi
Name:Syt2Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:99666 Syt2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 60VesicularSequence analysisAdd BLAST60
Transmembranei61 – 87HelicalSequence analysisAdd BLAST27
Topological domaini88 – 422CytoplasmicSequence analysisAdd BLAST335

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47F → A: No binding to C.botulinum neurotoxin type B (BoNT/B, botB). Requires gangliosides to bind BoNT/B, wild-type binding to BoNT/G. 2 Publications1
Mutagenesisi48 – 52AKLKE → SKLKQ: Wild-type binding to BoNT/B. 1 Publication5
Mutagenesisi50L → A: Wild-type in binding to C.botulinum neurotoxin type G (BoNT/G, botG). 1 Publication1
Mutagenesisi52 – 55EKFF → QKFM: Wild-type binding to BoNT/B. Wild-type in binding to BoNT/G. 2 Publications4
Mutagenesisi52E → A: Wild-type binding to BoNT/B. 1 Publication1
Mutagenesisi54F → A: No binding to BoNT/B. No binding to BoNT/G. 2 Publications1
Mutagenesisi54F → M: Requires gangliosides to bind BoNT/B, no binding to BoNT/G with or without gangliosides. 1 Publication1
Mutagenesisi55F → A: No binding to BoNT/B. Wild-type binding to BoNT/G. 2 Publications1
Mutagenesisi58 – 59IN → LH: Only binds to BoNT/B in presence of gangliosides. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839431 – 422Synaptotagmin-2Add BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi32N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei125PhosphothreonineBy similarity1
Modified residuei128PhosphothreonineCombined sources1
Modified residuei230PhosphotyrosineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP46097
PaxDbiP46097
PeptideAtlasiP46097
PRIDEiP46097

PTM databases

iPTMnetiP46097
PhosphoSitePlusiP46097
SwissPalmiP46097

Expressioni

Gene expression databases

CleanExiMM_SYT2

Interactioni

Subunit structurei

Homotetramer (Probable). Interacts with SCAMP5 (By similarity). Interacts with STON2 (By similarity). Interacts with PRRT2 (PubMed:27052163).By similarityCurated1 Publication
(Microbial infection) Interacts with C.botulinum neurotoxin type B (BoNT/B, botB).3 Publications
(Microbial infection) Interacts with C.botulinum neurotoxin type G (BoNT/G, botG).1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203612, 4 interactors
DIPiDIP-32645N
IntActiP46097, 11 interactors
MINTiP46097
STRINGi10090.ENSMUSP00000112438

Structurei

Secondary structure

1422
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP46097
SMRiP46097
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46097

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 245C2 1PROSITE-ProRule annotationAdd BLAST90
Domaini287 – 378C2 2PROSITE-ProRule annotationAdd BLAST92

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 382Phospholipid bindingCuratedAdd BLAST247

Domaini

The first C2 domain mediates Ca2+-dependent phospholipid binding (PubMed:7961887).
The second C2 domain mediates interaction with Stonin 2. The second C2 domain mediates phospholipid and inositol polyphosphate binding in a calcium-independent manner (PubMed:7961887).1 Publication
(Microbial infection) Binding to BoNT/B induces formation of an alpha-helix in the membrane-proximal extracytoplasmic domain (PubMed:17167418, PubMed:23807078).2 Publications

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1028 Eukaryota
ENOG4111B9R LUCA
HOGENOMiHOG000232127
HOVERGENiHBG005010
InParanoidiP46097
KOiK19902
PhylomeDBiP46097
TreeFamiTF315600

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001565 Synaptotagmin
IPR015428 Synaptotagmin1/2
PANTHERiPTHR10024:SF223 PTHR10024:SF223, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P46097-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRNIFKRNQE PNVAPATTTA TMPLAPVAPA DNSTESTGPG ESQEDMFAKL
60 70 80 90 100
KEKFFNEINK IPLPPWALIA MAVVAGLLLL TCCFCICKKC CCKKKKNKKE
110 120 130 140 150
KGKGMKNAMN MKDMKGGQDD DDAETGLTEG EGEGEEEKEP ENLGKLQFSL
160 170 180 190 200
DYDFQANQLT VGVLQAAELP ALDMGGTSDP YVKVFLLPDK KKKYETKVHR
210 220 230 240 250
KTLNPAFNET FTFKVPYQEL AGKTLVMAIY DFDRFSKHDI IGEVKVPMNT
260 270 280 290 300
VDLGQPIEEW RDLQGGEKEE PEKLGDICTS LRYVPTAGKL TVCILEAKNL
310 320 330 340 350
KKMDVGGLSD PYVKIHLMQN GKRLKKKKTT VKKKTLNPYF NESFSFEIPF
360 370 380 390 400
EQIQKVQVVV TVLDYDKLGK NEAIGKIFVG SNATGTELRH WSDMLANPRR
410 420
PIAQWHSLKP EEEVDALLGK NK
Length:422
Mass (Da):47,263
Last modified:November 1, 1995 - v1
Checksum:iB4BD13FF70E0481B
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J2C2A0A0R4J2C2_MOUSE
Synaptotagmin II
Syt2 mCG_5342
422Annotation score:
Q9JM87Q9JM87_MOUSE
Synaptotagmin II
Syt2 syt II
69Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti221A → G in BAC29397 (PubMed:16141072).Curated1
Sequence conflicti221A → G in AAH27019 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37793 mRNA Translation: BAA07041.1
AF257303 Genomic DNA Translation: AAF68987.1
AF257304 mRNA Translation: AAF68988.1
AK036357 mRNA Translation: BAC29397.1
BC027019 mRNA Translation: AAH27019.1
CCDSiCCDS15312.1
PIRiA55417
RefSeqiNP_033333.2, NM_009307.3
XP_006529385.1, XM_006529322.1
XP_006529386.1, XM_006529323.3
UniGeneiMm.5102

Genome annotation databases

GeneIDi20980
KEGGimmu:20980
UCSCiuc007csj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37793 mRNA Translation: BAA07041.1
AF257303 Genomic DNA Translation: AAF68987.1
AF257304 mRNA Translation: AAF68988.1
AK036357 mRNA Translation: BAC29397.1
BC027019 mRNA Translation: AAH27019.1
CCDSiCCDS15312.1
PIRiA55417
RefSeqiNP_033333.2, NM_009307.3
XP_006529385.1, XM_006529322.1
XP_006529386.1, XM_006529323.3
UniGeneiMm.5102

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NP0X-ray2.62B40-60[»]
4KBBX-ray2.30C/D8-61[»]
ProteinModelPortaliP46097
SMRiP46097
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203612, 4 interactors
DIPiDIP-32645N
IntActiP46097, 11 interactors
MINTiP46097
STRINGi10090.ENSMUSP00000112438

PTM databases

iPTMnetiP46097
PhosphoSitePlusiP46097
SwissPalmiP46097

Proteomic databases

MaxQBiP46097
PaxDbiP46097
PeptideAtlasiP46097
PRIDEiP46097

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20980
KEGGimmu:20980
UCSCiuc007csj.1 mouse

Organism-specific databases

CTDi127833
MGIiMGI:99666 Syt2

Phylogenomic databases

eggNOGiKOG1028 Eukaryota
ENOG4111B9R LUCA
HOGENOMiHOG000232127
HOVERGENiHBG005010
InParanoidiP46097
KOiK19902
PhylomeDBiP46097
TreeFamiTF315600

Miscellaneous databases

EvolutionaryTraceiP46097
PROiPR:P46097
SOURCEiSearch...

Gene expression databases

CleanExiMM_SYT2

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001565 Synaptotagmin
IPR015428 Synaptotagmin1/2
PANTHERiPTHR10024:SF223 PTHR10024:SF223, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSYT2_MOUSE
AccessioniPrimary (citable) accession number: P46097
Secondary accession number(s): Q8R0E1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 7, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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