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Protein

Synaptotagmin-1

Gene

Syt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (PubMed:7961887). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (By similarity).By similarity1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi171Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi172Calcium 1By similarity1
Metal bindingi172Calcium 2By similarity1
Metal bindingi178Calcium 1By similarity1
Metal bindingi230Calcium 1By similarity1
Metal bindingi230Calcium 2By similarity1
Metal bindingi231Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi232Calcium 1By similarity1
Metal bindingi232Calcium 2By similarity1
Metal bindingi232Calcium 3By similarity1
Metal bindingi235Calcium 3By similarity1
Metal bindingi236Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi238Calcium 2By similarity1
Metal bindingi238Calcium 3By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDifferentiation
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-181429 Serotonin Neurotransmitter Release Cycle
R-MMU-181430 Norepinephrine Neurotransmitter Release Cycle
R-MMU-210500 Glutamate Neurotransmitter Release Cycle
R-MMU-212676 Dopamine Neurotransmitter Release Cycle
R-MMU-264642 Acetylcholine Neurotransmitter Release Cycle
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis
R-MMU-888590 GABA synthesis, release, reuptake and degradation

Protein family/group databases

TCDBi9.A.48.1.1 the unconventional protein secretion (ups) system

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-1
Alternative name(s):
Synaptotagmin I
Short name:
SytI
p65
Gene namesi
Name:Syt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:99667 Syt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 57VesicularSequence analysisAdd BLAST57
Transmembranei58 – 79HelicalSequence analysisAdd BLAST22
Topological domaini80 – 421CytoplasmicSequence analysisAdd BLAST342

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839381 – 421Synaptotagmin-1Add BLAST421

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...) asparagineBy similarity1
Lipidationi74S-palmitoyl cysteineBy similarity1
Lipidationi75S-palmitoyl cysteineBy similarity1
Lipidationi77S-palmitoyl cysteineBy similarity1
Lipidationi79S-palmitoyl cysteineBy similarity1
Lipidationi82S-palmitoyl cysteineBy similarity1
Modified residuei128PhosphothreonineBy similarity1
Modified residuei229PhosphotyrosineCombined sources1
Modified residuei264PhosphoserineCombined sources1
Modified residuei342PhosphoserineBy similarity1
Modified residuei344PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP46096
PaxDbiP46096
PRIDEiP46096

PTM databases

iPTMnetiP46096
PhosphoSitePlusiP46096
SwissPalmiP46096

Expressioni

Gene expression databases

BgeeiENSMUSG00000035864 Expressed in 239 organ(s), highest expression level in hypothalamus
CleanExiMM_SYT1
ExpressionAtlasiP46096 baseline and differential
GenevisibleiP46096 MM

Interactioni

Subunit structurei

Homotetramer (Probable). Interacts with SCAMP5, STON2, SV2A, SV2B, SV2C and RIMS1 (By similarity). Forms a complex with SV2B, syntaxin 1 and SNAP25. Interacts with PRRT2 (PubMed:27052163).By similarityCurated2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203611, 7 interactors
DIPiDIP-31322N
IntActiP46096, 20 interactors
MINTiP46096
STRINGi10090.ENSMUSP00000063293

Structurei

Secondary structure

1421
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP46096
SMRiP46096
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini143 – 244C2 1PROSITE-ProRule annotationAdd BLAST102
Domaini274 – 377C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni135 – 381Phospholipid bindingCuratedAdd BLAST247

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi80 – 119Lys-richAdd BLAST40

Domaini

The first C2 domain mediates Ca2+-dependent phospholipid binding.By similarity
The second C2 domain mediates interaction with SV2A and probably with STN2.By similarity

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IT3Q Eukaryota
ENOG410Z15P LUCA
GeneTreeiENSGT00760000118973
HOGENOMiHOG000232127
HOVERGENiHBG005010
InParanoidiP46096
KOiK15290
OMAiHDIIGEY
OrthoDBiEOG091G0XMQ
PhylomeDBiP46096
TreeFamiTF315600

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001565 Synaptotagmin
IPR015428 Synaptotagmin1/2
PANTHERiPTHR10024:SF239 PTHR10024:SF239, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits

Sequencei

Sequence statusi: Complete.

P46096-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVSASRPEAL AAPVTTVATL VPHNATEPAS PGEGKEDAFS KLKQKFMNEL
60 70 80 90 100
HKIPLPPWAL IAIAIVAVLL VVTCCFCVCK KCLFKKKNKK KGKEKGGKNA
110 120 130 140 150
INMKDVKDLG KTMKDQALKD DDAETGLTDG EEKEEPKEEE KLGKLQYSLD
160 170 180 190 200
YDFQNNQLLV GIIQAAELPA LDMGGTSDPY VKVFLLPDKK KKFETKVHRK
210 220 230 240 250
TLNPVFNEQF TFKVPYSELG GKTLVMAVYD FDRFSKHDII GEFKVPMNTV
260 270 280 290 300
DFGHVTEEWR DLQSAEKEEQ EKLGDICFSL RYVPTAGKLT VVILEAKNLK
310 320 330 340 350
KMDVGGLSDP YVKIHLMQNG KRLKKKKTTI KKNTLNPYYN ESFSFEVPFE
360 370 380 390 400
QIQKVQVVVT VLDYDKIGKN DAIGKVFVGY NSTGAELRHW SDMLANPRRP
410 420
IAQWHTLQVE EEVDAMLAVK K
Length:421
Mass (Da):47,418
Last modified:November 1, 1995 - v1
Checksum:i7FDEFF37170BD169
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37792 mRNA Translation: BAA07040.1
AK078790 mRNA Translation: BAC37395.1
BC042519 mRNA Translation: AAH42519.1
CCDSiCCDS24163.1
RefSeqiNP_001239270.1, NM_001252341.1
NP_001239271.1, NM_001252342.1
NP_033332.1, NM_009306.3
XP_006513509.1, XM_006513446.1
XP_006513510.1, XM_006513447.1
XP_006513511.1, XM_006513448.1
XP_017169355.1, XM_017313866.1
UniGeneiMm.289702
Mm.489829

Genome annotation databases

EnsembliENSMUST00000064054; ENSMUSP00000063293; ENSMUSG00000035864
ENSMUST00000105276; ENSMUSP00000100912; ENSMUSG00000035864
GeneIDi20979
KEGGimmu:20979
UCSCiuc007gzg.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37792 mRNA Translation: BAA07040.1
AK078790 mRNA Translation: BAC37395.1
BC042519 mRNA Translation: AAH42519.1
CCDSiCCDS24163.1
RefSeqiNP_001239270.1, NM_001252341.1
NP_001239271.1, NM_001252342.1
NP_033332.1, NM_009306.3
XP_006513509.1, XM_006513446.1
XP_006513510.1, XM_006513447.1
XP_006513511.1, XM_006513448.1
XP_017169355.1, XM_017313866.1
UniGeneiMm.289702
Mm.489829

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5T0RX-ray1.95A140-265[»]
5T0SX-ray1.42A271-421[»]
5VFEX-ray1.38A/B140-265[»]
5VFFX-ray1.41A271-421[»]
5VFGX-ray1.82A271-421[»]
ProteinModelPortaliP46096
SMRiP46096
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203611, 7 interactors
DIPiDIP-31322N
IntActiP46096, 20 interactors
MINTiP46096
STRINGi10090.ENSMUSP00000063293

Protein family/group databases

TCDBi9.A.48.1.1 the unconventional protein secretion (ups) system

PTM databases

iPTMnetiP46096
PhosphoSitePlusiP46096
SwissPalmiP46096

Proteomic databases

MaxQBiP46096
PaxDbiP46096
PRIDEiP46096

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064054; ENSMUSP00000063293; ENSMUSG00000035864
ENSMUST00000105276; ENSMUSP00000100912; ENSMUSG00000035864
GeneIDi20979
KEGGimmu:20979
UCSCiuc007gzg.2 mouse

Organism-specific databases

CTDi6857
MGIiMGI:99667 Syt1

Phylogenomic databases

eggNOGiENOG410IT3Q Eukaryota
ENOG410Z15P LUCA
GeneTreeiENSGT00760000118973
HOGENOMiHOG000232127
HOVERGENiHBG005010
InParanoidiP46096
KOiK15290
OMAiHDIIGEY
OrthoDBiEOG091G0XMQ
PhylomeDBiP46096
TreeFamiTF315600

Enzyme and pathway databases

ReactomeiR-MMU-181429 Serotonin Neurotransmitter Release Cycle
R-MMU-181430 Norepinephrine Neurotransmitter Release Cycle
R-MMU-210500 Glutamate Neurotransmitter Release Cycle
R-MMU-212676 Dopamine Neurotransmitter Release Cycle
R-MMU-264642 Acetylcholine Neurotransmitter Release Cycle
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis
R-MMU-888590 GABA synthesis, release, reuptake and degradation

Miscellaneous databases

ChiTaRSiSyt1 mouse
PROiPR:P46096
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035864 Expressed in 239 organ(s), highest expression level in hypothalamus
CleanExiMM_SYT1
ExpressionAtlasiP46096 baseline and differential
GenevisibleiP46096 MM

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001565 Synaptotagmin
IPR015428 Synaptotagmin1/2
PANTHERiPTHR10024:SF239 PTHR10024:SF239, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSYT1_MOUSE
AccessioniPrimary (citable) accession number: P46096
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 7, 2018
This is version 180 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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