UniProtKB - P46087 (NOP2_HUMAN)
Protein
Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase
Gene
NOP2
Organism
Homo sapiens (Human)
Status
Functioni
Involved in ribosomal large subunit assembly (PubMed:24120868). S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 4447 in 28S rRNA (Probable). May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation (Probable).Curated1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 416 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 443 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 460 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Active sitei | 517 | NucleophilePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- RNA binding Source: UniProtKB
- rRNA (cytosine-C5-)-methyltransferase activity Source: GO_Central
GO - Biological processi
- maturation of LSU-rRNA Source: GO_Central
- positive regulation of cell population proliferation Source: GO_Central
- regulation of signal transduction by p53 class mediator Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: Reactome
- ribosomal large subunit assembly Source: UniProtKB
- RNA methylation Source: GO_Central
- rRNA base methylation Source: GO_Central
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase |
Biological process | Ribosome biogenesis, rRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
PathwayCommonsi | P46087 |
Reactomei | R-HSA-6790901, rRNA modification in the nucleus and cytosol R-HSA-8869496, TFAP2A acts as a transcriptional repressor during retinoic acid induced cell differentiation |
Names & Taxonomyi
Protein namesi | Recommended name: Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase (EC:2.1.1.-1 Publication)Alternative name(s): Nucleolar protein 1 Nucleolar protein 2 homolog Proliferating-cell nucleolar antigen p120 Proliferation-associated nucleolar protein p120 |
Gene namesi | Name:NOP2 Synonyms:NOL1, NSUN1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7867, NOP2 |
MIMi | 164031, gene |
neXtProti | NX_P46087 |
VEuPathDBi | HostDB:ENSG00000111641.10 |
Subcellular locationi
Nucleus
- nucleolus 1 Publication
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: Reactome
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 4839 |
OpenTargetsi | ENSG00000111641 |
PharmGKBi | PA164724026 |
Miscellaneous databases
Pharosi | P46087, Tbio |
Genetic variation databases
BioMutai | NOP2 |
DMDMi | 146289861 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211818 | 1 – 812 | Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferaseAdd BLAST | 812 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 36 | PhosphoserineCombined sources | 1 | |
Modified residuei | 58 | PhosphoserineCombined sources | 1 | |
Modified residuei | 67 | PhosphoserineCombined sources | 1 | |
Cross-linki | 71 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 102 | CitrullineBy similarity | 1 | |
Modified residuei | 164 | CitrullineBy similarity | 1 | |
Modified residuei | 181 | PhosphoserineCombined sources | 1 | |
Modified residuei | 185 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 195 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 272 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 615 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 649 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 666 | PhosphoserineCombined sources | 1 | |
Modified residuei | 675 | PhosphoserineCombined sources | 1 | |
Modified residuei | 732 | PhosphoserineCombined sources | 1 | |
Modified residuei | 734 | PhosphoserineCombined sources | 1 | |
Modified residuei | 739 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 776 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 786 | PhosphoserineCombined sources | 1 | |
Modified residuei | 801 | PhosphoserineCombined sources | 1 | |
Modified residuei | 812 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Citrullinated by PADI4.By similarity
Keywords - PTMi
Acetylation, Citrullination, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P46087 |
jPOSTi | P46087 |
MassIVEi | P46087 |
MaxQBi | P46087 |
PaxDbi | P46087 |
PeptideAtlasi | P46087 |
PRIDEi | P46087 |
ProteomicsDBi | 55714 [P46087-1] 55715 [P46087-2] 58366 61721 |
TopDownProteomicsi | P46087-2 [P46087-2] |
2D gel databases
SWISS-2DPAGEi | P46087 |
PTM databases
GlyGeni | P46087, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P46087 |
PhosphoSitePlusi | P46087 |
SwissPalmi | P46087 |
Expressioni
Developmental stagei
Expressed in G1 and peaks during the early S phase of the cell cycle.1 Publication
Gene expression databases
Bgeei | ENSG00000111641, Expressed in left lobe of thyroid gland and 129 other tissues |
ExpressionAtlasi | P46087, baseline and differential |
Genevisiblei | P46087, HS |
Organism-specific databases
HPAi | ENSG00000111641, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsP46087
With | #Exp. | IntAct |
---|---|---|
CDCA7L [Q96GN5] | 3 | EBI-356811,EBI-5278764 |
CEP70 [Q8NHQ1] | 3 | EBI-356811,EBI-739624 |
KPNA6 [O60684] | 3 | EBI-356811,EBI-359923 |
MT1F [P04733] | 3 | EBI-356811,EBI-10209483 |
VPS52 [Q8N1B4] | 3 | EBI-356811,EBI-2799833 |
Protein-protein interaction databases
BioGRIDi | 110902, 250 interactors |
IntActi | P46087, 109 interactors |
MINTi | P46087 |
STRINGi | 9606.ENSP00000371858 |
Miscellaneous databases
RNActi | P46087, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 392 – 398 | S-adenosyl-L-methionine bindingPROSITE-ProRule annotation | 7 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG1122, Eukaryota |
GeneTreei | ENSGT00940000155370 |
HOGENOMi | CLU_005316_2_0_1 |
InParanoidi | P46087 |
OMAi | VQRCYNL |
OrthoDBi | 1528896at2759 |
PhylomeDBi | P46087 |
TreeFami | TF105660 |
Family and domain databases
InterProi | View protein in InterPro IPR018314, Fmu/NOL1/Nop2p_CS IPR031341, Methyltr_RsmF_N IPR001678, MeTrfase_RsmB/NOP2 IPR011023, Nop2p IPR012586, P120R_rpt IPR023267, RCMT IPR023273, RCMT_NOP2 IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF01189, Methyltr_RsmB-F, 1 hit PF17125, Methyltr_RsmF_N, 1 hit PF08062, P120R, 3 hits |
PRINTSi | PR02008, RCMTFAMILY PR02012, RCMTNOP2 |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00446, nop2p, 1 hit |
PROSITEi | View protein in PROSITE PS01153, NOL1_NOP2_SUN, 1 hit PS51686, SAM_MT_RSMB_NOP, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P46087-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGRKLDPTKE KRGPGRKARK QKGAETELVR FLPAVSDENS KRLSSRARKR
60 70 80 90 100
AAKRRLGSVE APKTNKSPEA KPLPGKLPKG ISAGAVQTAG KKGPQSLFNA
110 120 130 140 150
PRGKKRPAPG SDEEEEEEDS EEDGMVNHGD LWGSEDDADT VDDYGADSNS
160 170 180 190 200
EDEEEGEALL PIERAARKQK AREAAAGIQW SEEETEDEEE EKEVTPESGP
210 220 230 240 250
PKVEEADGGL QINVDEEPFV LPPAGEMEQD AQAPDLQRVH KRIQDIVGIL
260 270 280 290 300
RDFGAQREEG RSRSEYLNRL KKDLAIYYSY GDFLLGKLMD LFPLSELVEF
310 320 330 340 350
LEANEVPRPV TLRTNTLKTR RRDLAQALIN RGVNLDPLGK WSKTGLVVYD
360 370 380 390 400
SSVPIGATPE YLAGHYMLQG ASSMLPVMAL APQEHERILD MCCAPGGKTS
410 420 430 440 450
YMAQLMKNTG VILANDANAE RLKSVVGNLH RLGVTNTIIS HYDGRQFPKV
460 470 480 490 500
VGGFDRVLLD APCSGTGVIS KDPAVKTNKD EKDILRCAHL QKELLLSAID
510 520 530 540 550
SVNATSKTGG YLVYCTCSIT VEENEWVVDY ALKKRNVRLV PTGLDFGQEG
560 570 580 590 600
FTRFRERRFH PSLRSTRRFY PHTHNMDGFF IAKFKKFSNS IPQSQTGNSE
610 620 630 640 650
TATPTNVDLP QVIPKSENSS QPAKKAKGAA KTKQQLQKQQ HPKKASFQKL
660 670 680 690 700
NGISKGADSE LSTVPSVTKT QASSSFQDSS QPAGKAEGIR EPKVTGKLKQ
710 720 730 740 750
RSPKLQSSKK VAFLRQNAPP KGTDTQTPAV LSPSKTQATL KPKDHHQPLG
760 770 780 790 800
RAKGVEKQQL PEQPFEKAAF QKQNDTPKGP QPPTVSPIRS SRPPPAKRKK
810
SQSRGNSQLL LS
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5GYR3 | F5GYR3_HUMAN | Probable 28S rRNA (cytosine(4447)-C... | NOP2 | 275 | Annotation score: | ||
F5H359 | F5H359_HUMAN | Probable 28S rRNA (cytosine(4447)-C... | NOP2 | 81 | Annotation score: | ||
F5H5X6 | F5H5X6_HUMAN | Probable 28S rRNA (cytosine(4447)-C... | NOP2 | 250 | Annotation score: | ||
F5GWB7 | F5GWB7_HUMAN | Probable 28S rRNA (cytosine(4447)-C... | NOP2 | 159 | Annotation score: | ||
F5H709 | F5H709_HUMAN | Probable 28S rRNA (cytosine(4447)-C... | NOP2 | 176 | Annotation score: | ||
F5H8G6 | F5H8G6_HUMAN | Probable 28S rRNA (cytosine(4447)-C... | NOP2 | 98 | Annotation score: |
Sequence cautioni
The sequence AAA36398 differs from that shown. Reason: Frameshift.Curated
The sequence CAA39119 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 559 | F → L in AAI28185 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 630 | A → G in AAA36398 (PubMed:2576976).Curated | 1 | |
Sequence conflicti | 760 – 761 | LP → FA in AAA36398 (PubMed:2576976).Curated | 2 | |
Sequence conflicti | 760 – 761 | LP → FA in CAA39119 (PubMed:1394192).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_030938 | 73 | L → S2 PublicationsCorresponds to variant dbSNP:rs1128164Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023494 | 81 – 84 | Missing in isoform 2 and isoform 3. 2 Publications | 4 | |
Alternative sequenceiVSP_045308 | 158 | A → AAGVQWLGLGSLQPPPPGFK QFSCLSFPSSWDLQ in isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_045309 | 597 – 812 | GNSET…QLLLS → DGVLLCRSGWTAVVQSQLIA TSTFQVQAILVPQTPK in isoform 3. 1 PublicationAdd BLAST | 216 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32110 mRNA Translation: AAA36398.1 Sequence problems. M33132 Genomic DNA No translation available. X55504 mRNA Translation: CAA39119.1 Frameshift. AK056208 mRNA Translation: BAG51648.1 AC006064 Genomic DNA No translation available. BC082985 mRNA Translation: AAH82985.1 BC000656 mRNA Translation: AAH00656.1 BC065257 mRNA Translation: AAH65257.1 BC106072 mRNA Translation: AAI06073.1 BC128183 mRNA Translation: AAI28184.1 BC128184 mRNA Translation: AAI28185.1 |
CCDSi | CCDS44811.1 [P46087-2] CCDS58202.1 [P46087-3] CCDS58203.1 [P46087-1] CCDS58204.1 [P46087-4] |
PIRi | A48168 |
RefSeqi | NP_001028886.1, NM_001033714.2 [P46087-2] NP_001245237.1, NM_001258308.1 [P46087-1] NP_001245238.1, NM_001258309.1 [P46087-4] NP_001245239.1, NM_001258310.1 [P46087-3] NP_006161.2, NM_006170.3 [P46087-2] XP_005253748.1, XM_005253691.1 [P46087-1] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32110 mRNA Translation: AAA36398.1 Sequence problems. M33132 Genomic DNA No translation available. X55504 mRNA Translation: CAA39119.1 Frameshift. AK056208 mRNA Translation: BAG51648.1 AC006064 Genomic DNA No translation available. BC082985 mRNA Translation: AAH82985.1 BC000656 mRNA Translation: AAH00656.1 BC065257 mRNA Translation: AAH65257.1 BC106072 mRNA Translation: AAI06073.1 BC128183 mRNA Translation: AAI28184.1 BC128184 mRNA Translation: AAI28185.1 |
CCDSi | CCDS44811.1 [P46087-2] CCDS58202.1 [P46087-3] CCDS58203.1 [P46087-1] CCDS58204.1 [P46087-4] |
PIRi | A48168 |
RefSeqi | NP_001028886.1, NM_001033714.2 [P46087-2] NP_001245237.1, NM_001258308.1 [P46087-1] NP_001245238.1, NM_001258309.1 [P46087-4] NP_001245239.1, NM_001258310.1 [P46087-3] NP_006161.2, NM_006170.3 [P46087-2] XP_005253748.1, XM_005253691.1 [P46087-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 110902, 250 interactors |
IntActi | P46087, 109 interactors |
MINTi | P46087 |
STRINGi | 9606.ENSP00000371858 |
PTM databases
GlyGeni | P46087, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P46087 |
PhosphoSitePlusi | P46087 |
SwissPalmi | P46087 |
Genetic variation databases
BioMutai | NOP2 |
DMDMi | 146289861 |
2D gel databases
SWISS-2DPAGEi | P46087 |
Proteomic databases
EPDi | P46087 |
jPOSTi | P46087 |
MassIVEi | P46087 |
MaxQBi | P46087 |
PaxDbi | P46087 |
PeptideAtlasi | P46087 |
PRIDEi | P46087 |
ProteomicsDBi | 55714 [P46087-1] 55715 [P46087-2] 58366 61721 |
TopDownProteomicsi | P46087-2 [P46087-2] |
Protocols and materials databases
Antibodypediai | 22530, 101 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 4839 |
DisGeNETi | 4839 |
GeneCardsi | NOP2 |
HGNCi | HGNC:7867, NOP2 |
HPAi | ENSG00000111641, Low tissue specificity |
MIMi | 164031, gene |
neXtProti | NX_P46087 |
OpenTargetsi | ENSG00000111641 |
PharmGKBi | PA164724026 |
VEuPathDBi | HostDB:ENSG00000111641.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1122, Eukaryota |
GeneTreei | ENSGT00940000155370 |
HOGENOMi | CLU_005316_2_0_1 |
InParanoidi | P46087 |
OMAi | VQRCYNL |
OrthoDBi | 1528896at2759 |
PhylomeDBi | P46087 |
TreeFami | TF105660 |
Enzyme and pathway databases
PathwayCommonsi | P46087 |
Reactomei | R-HSA-6790901, rRNA modification in the nucleus and cytosol R-HSA-8869496, TFAP2A acts as a transcriptional repressor during retinoic acid induced cell differentiation |
Miscellaneous databases
BioGRID-ORCSi | 4839, 559 hits in 1007 CRISPR screens |
ChiTaRSi | NOP2, human |
GeneWikii | NOL1 |
GenomeRNAii | 4839 |
Pharosi | P46087, Tbio |
PROi | PR:P46087 |
RNActi | P46087, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000111641, Expressed in left lobe of thyroid gland and 129 other tissues |
ExpressionAtlasi | P46087, baseline and differential |
Genevisiblei | P46087, HS |
Family and domain databases
InterProi | View protein in InterPro IPR018314, Fmu/NOL1/Nop2p_CS IPR031341, Methyltr_RsmF_N IPR001678, MeTrfase_RsmB/NOP2 IPR011023, Nop2p IPR012586, P120R_rpt IPR023267, RCMT IPR023273, RCMT_NOP2 IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF01189, Methyltr_RsmB-F, 1 hit PF17125, Methyltr_RsmF_N, 1 hit PF08062, P120R, 3 hits |
PRINTSi | PR02008, RCMTFAMILY PR02012, RCMTNOP2 |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00446, nop2p, 1 hit |
PROSITEi | View protein in PROSITE PS01153, NOL1_NOP2_SUN, 1 hit PS51686, SAM_MT_RSMB_NOP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NOP2_HUMAN | |
Accessioni | P46087Primary (citable) accession number: P46087 Secondary accession number(s): A1A4Z3 Q58F30 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | March 6, 2007 | |
Last modified: | April 7, 2021 | |
This is version 198 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families