UniProtKB - P45983 (MK08_HUMAN)
Mitogen-activated protein kinase 8
MAPK8
Functioni
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed:18307971).
Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins (PubMed:21856198).
Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 (PubMed:21364637).
In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (PubMed:21095239).
Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed:18570871).
Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH (PubMed:20027304, PubMed:17296730, PubMed:16581800).
Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692).
Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed:10747973).
Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation (By similarity).
Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed:22327296).
In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity).
Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (PubMed:22966201).
By similarity12 PublicationsJNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
Catalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 55 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 151 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 32 – 40 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- enzyme binding Source: BHF-UCL
- histone deacetylase binding Source: BHF-UCL
- histone deacetylase regulator activity Source: BHF-UCL
- JUN kinase activity Source: UniProtKB
- MAP kinase activity Source: GO_Central
- protein phosphatase binding Source: UniProtKB
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine/threonine kinase binding Source: UniProtKB
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cellular response to amino acid starvation Source: CAFA
- cellular response to cadmium ion Source: CAFA
- cellular response to lipopolysaccharide Source: MGI
- cellular response to mechanical stimulus Source: UniProtKB
- cellular response to reactive oxygen species Source: CAFA
- cellular senescence Source: Reactome
- Fc-epsilon receptor signaling pathway Source: Reactome
- intracellular signal transduction Source: GO_Central
- JNK cascade Source: UniProtKB
- JUN phosphorylation Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of protein binding Source: UniProtKB
- peptidyl-serine phosphorylation Source: UniProtKB
- peptidyl-threonine phosphorylation Source: UniProtKB
- positive regulation of apoptotic process Source: GO_Central
- positive regulation of cell killing Source: Reactome
- positive regulation of cyclase activity Source: CACAO
- positive regulation of deacetylase activity Source: BHF-UCL
- positive regulation of gene expression Source: BHF-UCL
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
- positive regulation of protein metabolic process Source: CACAO
- protein phosphorylation Source: CAFA
- regulation of circadian rhythm Source: UniProtKB
- regulation of DNA-binding transcription factor activity Source: Reactome
- regulation of DNA replication origin binding Source: CAFA
- regulation of macroautophagy Source: ParkinsonsUK-UCL
- regulation of protein localization Source: BHF-UCL
- response to mechanical stimulus Source: GO_Central
- response to oxidative stress Source: UniProtKB
- response to UV Source: MGI
- rhythmic process Source: UniProtKB-KW
- stress-activated MAPK cascade Source: CAFA
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Biological rhythms |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 2681 |
PathwayCommonsi | P45983 |
Reactomei | R-HSA-111446, Activation of BIM and translocation to mitochondria R-HSA-139910, Activation of BMF and translocation to mitochondria R-HSA-193648, NRAGE signals death through JNK R-HSA-205043, NRIF signals cell death from the nucleus R-HSA-2559580, Oxidative Stress Induced Senescence R-HSA-2871796, FCERI mediated MAPK activation R-HSA-376172, DSCAM interactions R-HSA-450321, JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 R-HSA-450341, Activation of the AP-1 family of transcription factors R-HSA-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-HSA-9007892, Interleukin-38 signaling R-HSA-9673324, WNT5:FZD7-mediated leishmania damping |
SignaLinki | P45983 |
SIGNORi | P45983 |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase 8 (EC:2.7.11.247 Publications)Short name: MAP kinase 8 Short name: MAPK 8 Alternative name(s): JNK-46 Stress-activated protein kinase 1c Short name: SAPK1c Stress-activated protein kinase JNK1 c-Jun N-terminal kinase 1 |
Gene namesi | Name:MAPK8 Synonyms:JNK1, PRKM8, SAPK1, SAPK1C |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6881, MAPK8 |
MIMi | 601158, gene |
neXtProti | NX_P45983 |
VEuPathDBi | HostDB:ENSG00000107643 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- synapse By similarity
Note: In the cortical neurons, predominantly cytoplasmic and associated with the Golgi apparatus and endosomal fraction. Increased neuronal activity increases phosphorylated form at synapses (By similarity). Colocalizes with POU5F1 in the nucleus.By similarity
Cytosol
- cytosol Source: Reactome
Mitochondrion
- mitochondrion Source: GOC
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
Other locations
- axon Source: ARUK-UCL
- basal dendrite Source: ARUK-UCL
- cytoplasm Source: GO_Central
- synapse Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cytoplasm, Nucleus, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 55 | K → D: Abolished protein kinase activity. 1 Publication | 1 | |
Mutagenesisi | 183 | T → A: Phosphorylation blocked. 1 Publication | 1 | |
Mutagenesisi | 185 | Y → F: Phosphorylation blocked. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5599 |
OpenTargetsi | ENSG00000107643 |
PharmGKBi | PA283 |
Miscellaneous databases
Pharosi | P45983, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2276 |
DrugBanki | DB07268, 2-({2-[(3-HYDROXYPHENYL)AMINO]PYRIMIDIN-4-YL}AMINO)BENZAMIDE DB07845, 2-fluoro-6-{[2-({2-methoxy-4-[(methylsulfonyl)methyl]phenyl}amino)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]amino}benzamide DB07276, 5-CYANO-N-(2,5-DIMETHOXYBENZYL)-6-ETHOXYPYRIDINE-2-CARBOXAMIDE DB07218, 6-CHLORO-9-HYDROXY-1,3-DIMETHYL-1,9-DIHYDRO-4H-PYRAZOLO[3,4-B]QUINOLIN-4-ONE DB15624, Halicin DB01017, Minocycline DB07272, N-(4-AMINO-5-CYANO-6-ETHOXYPYRIDIN-2-YL)-2-(4-BROMO-2,5-DIMETHOXYPHENYL)ACETAMIDE DB01782, Pyrazolanthrone DB00675, Tamoxifen |
DrugCentrali | P45983 |
GuidetoPHARMACOLOGYi | 1496 |
Genetic variation databases
BioMutai | MAPK8 |
DMDMi | 2507195 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186262 | 1 – 427 | Mitogen-activated protein kinase 8Add BLAST | 427 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 116 | S-nitrosocysteineBy similarity | 1 | ||
Modified residuei | 183 | Phosphothreonine; by MAP2K71 Publication | 1 | ||
Modified residuei | 185 | Phosphotyrosine; by MAP2K41 Publication | 1 | ||
Modified residuei | 377 | PhosphoserineBy similarity | 1 | ||
Isoform 5 (identifier: P45983-5) | |||||
Modified residuei | 301 | PhosphoserineCombined sources | 1 | ||
Isoform 1 (identifier: P45983-2) | |||||
Modified residuei | 377 | PhosphoserineCombined sources | 1 | ||
Isoform 3 (identifier: P45983-3) | |||||
Modified residuei | 377 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, S-nitrosylationProteomic databases
CPTACi | CPTAC-889 CPTAC-890 CPTAC-891 CPTAC-892 |
EPDi | P45983 |
jPOSTi | P45983 |
MassIVEi | P45983 |
MaxQBi | P45983 |
PaxDbi | P45983 |
PeptideAtlasi | P45983 |
PRIDEi | P45983 |
ProteomicsDBi | 55694 [P45983-1] 55695 [P45983-2] 55696 [P45983-3] 55697 [P45983-4] 61569 |
PTM databases
iPTMneti | P45983 |
PhosphoSitePlusi | P45983 |
Expressioni
Gene expression databases
Bgeei | ENSG00000107643, Expressed in cortical plate and 223 other tissues |
ExpressionAtlasi | P45983, baseline and differential |
Genevisiblei | P45983, HS |
Organism-specific databases
HPAi | ENSG00000107643, Low tissue specificity |
Interactioni
Subunit structurei
Forms a complex with MAPK8IP1 and ARHGEF28 (By similarity).
Found in a complex with SH3RF1, RAC1, MAP3K11/MLK3, MAP2K7/MKK7 and MAPK8IP1/JIP1.
Found in a complex with SH3RF1, RAC2, MAP3K7/TAK1, MAP2K7/MKK7, MAPK8IP1/JIP1 and MAPK9/JNK2 (By similarity). Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4 (PubMed:15693750). These proteins also bind other components of the JNK signaling pathway.
Interacts with TP53 and WWOX (PubMed:12514174).
Interacts with JAMP (By similarity).
Interacts with HSF1 (via D domain and preferentially with hyperphosphorylated form); this interaction occurs under both normal growth conditions and immediately upon heat shock (PubMed:10747973).
Interacts (phosphorylated form) with NFE2; the interaction phosphorylates NFE2 in undifferentiated cells (By similarity).
Interacts with NFATC4 (PubMed:17875713).
Interacts with MECOM; regulates JNK signaling (PubMed:10856240).
Interacts with PIN1; this interaction mediates MAPK8 conformational changes leading to the binding of MAPK8 to its substrates (PubMed:21660049).
Interacts with GRIPAP1 (PubMed:17761173).
Interacts with POU5F1; phosphorylates POU5F1 at 'Ser-355'.
Interacts with STMN2, STMN3 and STMN4 (By similarity).
Interacts with HSF4 (PubMed:16581800).
By similarity9 PublicationsBinary interactionsi
P45983
Isoform 2 [P45983-1]
With | #Exp. | IntAct |
---|---|---|
JUN [P05412] | 2 | EBI-288687,EBI-852823 |
Isoform 4 [P45983-4]
With | #Exp. | IntAct |
---|---|---|
DUSP10 [Q9Y6W6] | 3 | EBI-18121963,EBI-3443946 |
DUSP16 [Q9BY84] | 3 | EBI-18121963,EBI-3443956 |
GO - Molecular functioni
- enzyme binding Source: BHF-UCL
- histone deacetylase binding Source: BHF-UCL
- protein phosphatase binding Source: UniProtKB
- protein serine/threonine kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 111585, 212 interactors |
DIPi | DIP-249N |
ELMi | P45983 |
IntActi | P45983, 107 interactors |
MINTi | P45983 |
STRINGi | 9606.ENSP00000378974 |
Chemistry databases
BindingDBi | P45983 |
Miscellaneous databases
RNActi | P45983, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P45983 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P45983 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 321 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 296 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 371 – 427 | DisorderedSequence analysisAdd BLAST | 57 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 183 – 185 | TXY | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 381 – 414 | Polar residuesSequence analysisAdd BLAST | 34 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0665, Eukaryota |
GeneTreei | ENSGT00940000153692 |
InParanoidi | P45983 |
OMAi | HHPYINV |
OrthoDBi | 741207at2759 |
PhylomeDBi | P45983 |
TreeFami | TF105100 |
Family and domain databases
IDEALi | IID00270 |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR008351, MAPK_JNK IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01772, JNKMAPKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSRSKRDNNF YSVEIGDSTF TVLKRYQNLK PIGSGAQGIV CAAYDAILER
60 70 80 90 100
NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKSLEE
110 120 130 140 150
FQDVYIVMEL MDANLCQVIQ MELDHERMSY LLYQMLCGIK HLHSAGIIHR
160 170 180 190 200
DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM
210 220 230 240 250
GYKENVDLWS VGCIMGEMVC HKILFPGRDY IDQWNKVIEQ LGTPCPEFMK
260 270 280 290 300
KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK
310 320 330 340 350
MLVIDASKRI SVDEALQHPY INVWYDPSEA EAPPPKIPDK QLDEREHTIE
360 370 380 390 400
EWKELIYKEV MDLEERTKNG VIRGQPSPLG AAVINGSQHP SSSSSVNDVS
410 420
SMSTDPTLAS DTDSSLEAAA GPLGCCR
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J762 | C9J762_HUMAN | Stress-activated protein kinase JNK | MAPK8 | 142 | Annotation score: | ||
A0A3B3IRW7 | A0A3B3IRW7_HUMAN | Stress-activated protein kinase JNK | MAPK8 | 169 | Annotation score: | ||
C9JWQ4 | C9JWQ4_HUMAN | Mitogen-activated protein kinase 8 | MAPK8 | 40 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_042258 | 171 | G → S in a renal clear cell carcinoma sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_042259 | 177 | G → R in a glioblastoma multiforme sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_050592 | 365 | E → K. Corresponds to variant dbSNP:rs45483593Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054554 | 206 – 281 | Missing in isoform 5. 1 PublicationAdd BLAST | 76 | |
Alternative sequenceiVSP_004831 | 208 | L → I in isoform 3 and isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_004832 | 219 – 230 | VCHKI…PGRDY → IKGGVLFPGTDH in isoform 3 and isoform 4. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_004833 | 380 – 427 | GAAVI…LGCCR → AQVQQ in isoform 1, isoform 3 and isoform 5. 3 PublicationsAdd BLAST | 48 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000360332; ENSP00000353483; ENSG00000107643 [P45983-5] ENST00000374176; ENSP00000363291; ENSG00000107643 [P45983-4] ENST00000374179; ENSP00000363294; ENSG00000107643 [P45983-3] ENST00000374182; ENSP00000363297; ENSG00000107643 [P45983-2] ENST00000374189; ENSP00000363304; ENSG00000107643 ENST00000395611; ENSP00000378974; ENSG00000107643 [P45983-4] |
GeneIDi | 5599 |
KEGGi | hsa:5599 |
MANE-Selecti | ENST00000374189.6; ENSP00000363304.1; NM_001323329.2; NP_001310258.1 |
UCSCi | uc001jgm.5, human [P45983-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Wikipedia C-Jun N-terminal kinases entry |
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1UKH | X-ray | 2.35 | A | 1-363 | [»] | |
1UKI | X-ray | 2.70 | A | 1-363 | [»] | |
2G01 | X-ray | 3.50 | A/B | 1-364 | [»] | |
2GMX | X-ray | 3.50 | A/B | 1-364 | [»] | |
2H96 | X-ray | 3.00 | A/B | 1-364 | [»] | |
2NO3 | X-ray | 3.20 | A/B | 1-364 | [»] | |
2XRW | X-ray | 1.33 | A | 2-364 | [»] | |
2XS0 | X-ray | 2.60 | A | 1-379 | [»] | |
3ELJ | X-ray | 1.80 | A | 1-364 | [»] | |
3O17 | X-ray | 3.00 | A/B | 1-364 | [»] | |
3O2M | X-ray | 2.70 | A/B | 1-364 | [»] | |
3PZE | X-ray | 2.00 | A | 7-364 | [»] | |
3V3V | X-ray | 2.70 | A | 1-366 | [»] | |
3VUD | X-ray | 3.50 | A | 1-364 | [»] | |
3VUG | X-ray | 3.24 | A | 1-364 | [»] | |
3VUH | X-ray | 2.70 | A | 1-364 | [»] | |
3VUI | X-ray | 2.80 | A | 1-364 | [»] | |
3VUK | X-ray | 2.95 | A | 1-364 | [»] | |
3VUL | X-ray | 2.81 | A | 1-364 | [»] | |
3VUM | X-ray | 2.69 | A | 1-364 | [»] | |
4AWI | X-ray | 1.91 | A | 1-364 | [»] | |
4E73 | X-ray | 2.27 | A | 1-363 | [»] | |
4G1W | X-ray | 2.45 | A | 1-363 | [»] | |
4HYS | X-ray | 2.42 | A | 1-363 | [»] | |
4HYU | X-ray | 2.15 | A | 1-363 | [»] | |
4IZY | X-ray | 2.30 | A | 1-363 | [»] | |
4L7F | X-ray | 1.95 | A | 7-362 | [»] | |
4QTD | X-ray | 1.50 | A | 1-363 | [»] | |
4UX9 | X-ray | 2.34 | A/B/C/D | 1-364 | [»] | |
4YR8 | X-ray | 2.40 | A/C/E/F | 1-363 | [»] | |
5LW1 | X-ray | 3.20 | B/E/H | 2-363 | [»] | |
6F5E | X-ray | 2.70 | B | 2-363 | [»] | |
6ZR5 | X-ray | 2.70 | A/B | 1-364 | [»] | |
SMRi | P45983 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111585, 212 interactors |
DIPi | DIP-249N |
ELMi | P45983 |
IntActi | P45983, 107 interactors |
MINTi | P45983 |
STRINGi | 9606.ENSP00000378974 |
Chemistry databases
BindingDBi | P45983 |
ChEMBLi | CHEMBL2276 |
DrugBanki | DB07268, 2-({2-[(3-HYDROXYPHENYL)AMINO]PYRIMIDIN-4-YL}AMINO)BENZAMIDE DB07845, 2-fluoro-6-{[2-({2-methoxy-4-[(methylsulfonyl)methyl]phenyl}amino)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]amino}benzamide DB07276, 5-CYANO-N-(2,5-DIMETHOXYBENZYL)-6-ETHOXYPYRIDINE-2-CARBOXAMIDE DB07218, 6-CHLORO-9-HYDROXY-1,3-DIMETHYL-1,9-DIHYDRO-4H-PYRAZOLO[3,4-B]QUINOLIN-4-ONE DB15624, Halicin DB01017, Minocycline DB07272, N-(4-AMINO-5-CYANO-6-ETHOXYPYRIDIN-2-YL)-2-(4-BROMO-2,5-DIMETHOXYPHENYL)ACETAMIDE DB01782, Pyrazolanthrone DB00675, Tamoxifen |
DrugCentrali | P45983 |
GuidetoPHARMACOLOGYi | 1496 |
PTM databases
iPTMneti | P45983 |
PhosphoSitePlusi | P45983 |
Genetic variation databases
BioMutai | MAPK8 |
DMDMi | 2507195 |
Proteomic databases
CPTACi | CPTAC-889 CPTAC-890 CPTAC-891 CPTAC-892 |
EPDi | P45983 |
jPOSTi | P45983 |
MassIVEi | P45983 |
MaxQBi | P45983 |
PaxDbi | P45983 |
PeptideAtlasi | P45983 |
PRIDEi | P45983 |
ProteomicsDBi | 55694 [P45983-1] 55695 [P45983-2] 55696 [P45983-3] 55697 [P45983-4] 61569 |
Protocols and materials databases
Antibodypediai | 3846, 1682 antibodies from 47 providers |
CPTCi | P45983, 1 antibody |
DNASUi | 5599 |
Genome annotation databases
Ensembli | ENST00000360332; ENSP00000353483; ENSG00000107643 [P45983-5] ENST00000374176; ENSP00000363291; ENSG00000107643 [P45983-4] ENST00000374179; ENSP00000363294; ENSG00000107643 [P45983-3] ENST00000374182; ENSP00000363297; ENSG00000107643 [P45983-2] ENST00000374189; ENSP00000363304; ENSG00000107643 ENST00000395611; ENSP00000378974; ENSG00000107643 [P45983-4] |
GeneIDi | 5599 |
KEGGi | hsa:5599 |
MANE-Selecti | ENST00000374189.6; ENSP00000363304.1; NM_001323329.2; NP_001310258.1 |
UCSCi | uc001jgm.5, human [P45983-1] |
Organism-specific databases
CTDi | 5599 |
DisGeNETi | 5599 |
GeneCardsi | MAPK8 |
HGNCi | HGNC:6881, MAPK8 |
HPAi | ENSG00000107643, Low tissue specificity |
MIMi | 601158, gene |
neXtProti | NX_P45983 |
OpenTargetsi | ENSG00000107643 |
PharmGKBi | PA283 |
VEuPathDBi | HostDB:ENSG00000107643 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0665, Eukaryota |
GeneTreei | ENSGT00940000153692 |
InParanoidi | P45983 |
OMAi | HHPYINV |
OrthoDBi | 741207at2759 |
PhylomeDBi | P45983 |
TreeFami | TF105100 |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 2681 |
PathwayCommonsi | P45983 |
Reactomei | R-HSA-111446, Activation of BIM and translocation to mitochondria R-HSA-139910, Activation of BMF and translocation to mitochondria R-HSA-193648, NRAGE signals death through JNK R-HSA-205043, NRIF signals cell death from the nucleus R-HSA-2559580, Oxidative Stress Induced Senescence R-HSA-2871796, FCERI mediated MAPK activation R-HSA-376172, DSCAM interactions R-HSA-450321, JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 R-HSA-450341, Activation of the AP-1 family of transcription factors R-HSA-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-HSA-9007892, Interleukin-38 signaling R-HSA-9673324, WNT5:FZD7-mediated leishmania damping |
SignaLinki | P45983 |
SIGNORi | P45983 |
Miscellaneous databases
BioGRID-ORCSi | 5599, 15 hits in 1082 CRISPR screens |
ChiTaRSi | MAPK8, human |
EvolutionaryTracei | P45983 |
GeneWikii | MAPK8 |
GenomeRNAii | 5599 |
Pharosi | P45983, Tchem |
PROi | PR:P45983 |
RNActi | P45983, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000107643, Expressed in cortical plate and 223 other tissues |
ExpressionAtlasi | P45983, baseline and differential |
Genevisiblei | P45983, HS |
Family and domain databases
IDEALi | IID00270 |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR008351, MAPK_JNK IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01772, JNKMAPKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MK08_HUMAN | |
Accessioni | P45983Primary (citable) accession number: P45983 Secondary accession number(s): B5BTZ5 Q308M2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 227 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families