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Protein

Chromobox protein homolog 5

Gene

CBX5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processHost-virus interaction

Enzyme and pathway databases

ReactomeiR-HSA-4551638 SUMOylation of chromatin organization proteins
R-HSA-8953750 Transcriptional Regulation by E2F6
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
SIGNORiP45973

Protein family/group databases

MoonDBiP45973 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
Chromobox protein homolog 5
Alternative name(s):
Antigen p25
Heterochromatin protein 1 homolog alpha
Short name:
HP1 alpha
Gene namesi
Name:CBX5
Synonyms:HP1A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000094916.13
HGNCiHGNC:1555 CBX5
MIMi604478 gene
neXtProtiNX_P45973

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi165I → E: No effect on interaction with POGZ. Abolishes interaction with TRIM28, CHAF1A and NIPBL. 1 Publication1
Mutagenesisi174W → A: Abolishes interaction with TRIM28, CHAF1A, NIPBL and HP1BP3, fails to localize to centromeres in mitosis. 3 Publications1

Organism-specific databases

DisGeNETi23468
OpenTargetsiENSG00000094916
PharmGKBiPA26130

Chemistry databases

ChEMBLiCHEMBL3826867

Polymorphism and mutation databases

BioMutaiCBX5
DMDMi1170338

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802081 – 191Chromobox protein homolog 5Add BLAST191

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11PhosphoserineCombined sources1
Modified residuei12PhosphoserineCombined sources1
Modified residuei13PhosphoserineCombined sources1
Modified residuei14PhosphoserineCombined sources1
Cross-linki32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei40N6-acetyllysineBy similarity1
Cross-linki91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei92PhosphoserineCombined sources1
Modified residuei95PhosphoserineCombined sources1
Modified residuei97PhosphoserineCombined sources1
Cross-linki102Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki106Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki184Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.By similarity1 Publication
Ubiquitinated.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP45973
PaxDbiP45973
PeptideAtlasiP45973
PRIDEiP45973
ProteomicsDBi55691
TopDownProteomicsiP45973

PTM databases

iPTMnetiP45973
PhosphoSitePlusiP45973

Expressioni

Gene expression databases

BgeeiENSG00000094916 Expressed in 227 organ(s), highest expression level in caput epididymis
CleanExiHS_CBX5
ExpressionAtlasiP45973 baseline and differential
GenevisibleiP45973 HS

Organism-specific databases

HPAiCAB017548
HPA016699

Interactioni

Subunit structurei

Interacts with KMT5B and KMT5C (By similarity). Interacts with HP1BP3 (PubMed:20042602). Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain (PubMed:15882967, PubMed:9169472). Can interact directly with CBX3 via the chromoshadow domain (PubMed:9169472). Interacts with histone H3 methylated at 'Lys-9' (PubMed:11242053, PubMed:21047797). Interacts with BAHD1, MIS12 and DSN1 (PubMed:15502821, PubMed:19666599). Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5 (PubMed:20562864, PubMed:20850016, PubMed:21346195). Interacts with INCENP and TRIM24 (PubMed:11313457). Interacts with CHAMP1 (PubMed:20850016). Interacts with ASXL1 (PubMed:19880879). Interacts with PRR14 (via N-terminus) (PubMed:25906157). Interacts with RRP1B (PubMed:19710015). Interacts with HNRNPU (via C-terminus); this interaction is, at least in part, RNA-dependent (PubMed:19617346). Interacts with LRIF1 (via PxVxL motif) (PubMed:23542155).By similarity17 Publications
(Microbial infection) Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi117030, 188 interactors
CORUMiP45973
DIPiDIP-5986N
IntActiP45973, 158 interactors
MINTiP45973
STRINGi9606.ENSP00000209875

Structurei

Secondary structure

1191
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP45973
SMRiP45973
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45973

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 78Chromo 1PROSITE-ProRule annotationAdd BLAST59
Domaini121 – 179Chromo 2; shadow subtypePROSITE-ProRule annotationAdd BLAST59

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 191Interaction with ASXL11 PublicationAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 14Poly-Ser4

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IUAB Eukaryota
ENOG4111I3N LUCA
GeneTreeiENSGT00510000046310
HOGENOMiHOG000220852
HOVERGENiHBG000400
InParanoidiP45973
KOiK11587
OMAiNVKIPQM
OrthoDBiEOG091G0RBS
PhylomeDBiP45973
TreeFamiTF350503

Family and domain databases

CDDicd00024 CHROMO, 1 hit
InterProiView protein in InterPro
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR017984 Chromo_dom_subgr
IPR023780 Chromo_domain
IPR008251 Chromo_shadow_dom
IPR023779 Chromodomain_CS
PfamiView protein in Pfam
PF00385 Chromo, 1 hit
PF01393 Chromo_shadow, 1 hit
PRINTSiPR00504 CHROMODOMAIN
SMARTiView protein in SMART
SM00298 CHROMO, 2 hits
SM00300 ChSh, 1 hit
SUPFAMiSSF54160 SSF54160, 2 hits
PROSITEiView protein in PROSITE
PS00598 CHROMO_1, 1 hit
PS50013 CHROMO_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P45973-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGKKTKRTAD SSSSEDEEEY VVEKVLDRRV VKGQVEYLLK WKGFSEEHNT
60 70 80 90 100
WEPEKNLDCP ELISEFMKKY KKMKEGENNK PREKSESNKR KSNFSNSADD
110 120 130 140 150
IKSKKKREQS NDIARGFERG LEPEKIIGAT DSCGDLMFLM KWKDTDEADL
160 170 180 190
VLAKEANVKC PQIVIAFYEE RLTWHAYPED AENKEKETAK S
Length:191
Mass (Da):22,225
Last modified:November 1, 1995 - v1
Checksum:i16FFC476367093B1
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VNY3F8VNY3_HUMAN
Chromobox protein homolog 5
CBX5
91Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S62077 mRNA Translation: AAB26994.1
L07515 mRNA Translation: AAA72327.1
AK313506 mRNA Translation: BAG36286.1
CH471054 Genomic DNA Translation: EAW96759.1
BC006821 mRNA Translation: AAH06821.1
U26311 mRNA Translation: AAC50553.1
CCDSiCCDS8875.1
PIRiG01808
RefSeqiNP_001120793.1, NM_001127321.1
NP_001120794.1, NM_001127322.1
NP_036249.1, NM_012117.2
UniGeneiHs.349283

Genome annotation databases

EnsembliENST00000209875; ENSP00000209875; ENSG00000094916
ENST00000439541; ENSP00000401009; ENSG00000094916
ENST00000550411; ENSP00000449207; ENSG00000094916
GeneIDi23468
KEGGihsa:23468

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S62077 mRNA Translation: AAB26994.1
L07515 mRNA Translation: AAA72327.1
AK313506 mRNA Translation: BAG36286.1
CH471054 Genomic DNA Translation: EAW96759.1
BC006821 mRNA Translation: AAH06821.1
U26311 mRNA Translation: AAC50553.1
CCDSiCCDS8875.1
PIRiG01808
RefSeqiNP_001120793.1, NM_001127321.1
NP_001120794.1, NM_001127322.1
NP_036249.1, NM_012117.2
UniGeneiHs.349283

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FDTX-ray2.00A18-75[»]
3I3CX-ray2.48A/B/C/D110-173[»]
ProteinModelPortaliP45973
SMRiP45973
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117030, 188 interactors
CORUMiP45973
DIPiDIP-5986N
IntActiP45973, 158 interactors
MINTiP45973
STRINGi9606.ENSP00000209875

Chemistry databases

ChEMBLiCHEMBL3826867

Protein family/group databases

MoonDBiP45973 Predicted

PTM databases

iPTMnetiP45973
PhosphoSitePlusiP45973

Polymorphism and mutation databases

BioMutaiCBX5
DMDMi1170338

Proteomic databases

EPDiP45973
PaxDbiP45973
PeptideAtlasiP45973
PRIDEiP45973
ProteomicsDBi55691
TopDownProteomicsiP45973

Protocols and materials databases

DNASUi23468
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000209875; ENSP00000209875; ENSG00000094916
ENST00000439541; ENSP00000401009; ENSG00000094916
ENST00000550411; ENSP00000449207; ENSG00000094916
GeneIDi23468
KEGGihsa:23468

Organism-specific databases

CTDi23468
DisGeNETi23468
EuPathDBiHostDB:ENSG00000094916.13
GeneCardsiCBX5
HGNCiHGNC:1555 CBX5
HPAiCAB017548
HPA016699
MIMi604478 gene
neXtProtiNX_P45973
OpenTargetsiENSG00000094916
PharmGKBiPA26130
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IUAB Eukaryota
ENOG4111I3N LUCA
GeneTreeiENSGT00510000046310
HOGENOMiHOG000220852
HOVERGENiHBG000400
InParanoidiP45973
KOiK11587
OMAiNVKIPQM
OrthoDBiEOG091G0RBS
PhylomeDBiP45973
TreeFamiTF350503

Enzyme and pathway databases

ReactomeiR-HSA-4551638 SUMOylation of chromatin organization proteins
R-HSA-8953750 Transcriptional Regulation by E2F6
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
SIGNORiP45973

Miscellaneous databases

ChiTaRSiCBX5 human
EvolutionaryTraceiP45973
GeneWikiiCBX5_(gene)
GenomeRNAii23468
PROiPR:P45973
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000094916 Expressed in 227 organ(s), highest expression level in caput epididymis
CleanExiHS_CBX5
ExpressionAtlasiP45973 baseline and differential
GenevisibleiP45973 HS

Family and domain databases

CDDicd00024 CHROMO, 1 hit
InterProiView protein in InterPro
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR017984 Chromo_dom_subgr
IPR023780 Chromo_domain
IPR008251 Chromo_shadow_dom
IPR023779 Chromodomain_CS
PfamiView protein in Pfam
PF00385 Chromo, 1 hit
PF01393 Chromo_shadow, 1 hit
PRINTSiPR00504 CHROMODOMAIN
SMARTiView protein in SMART
SM00298 CHROMO, 2 hits
SM00300 ChSh, 1 hit
SUPFAMiSSF54160 SSF54160, 2 hits
PROSITEiView protein in PROSITE
PS00598 CHROMO_1, 1 hit
PS50013 CHROMO_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCBX5_HUMAN
AccessioniPrimary (citable) accession number: P45973
Secondary accession number(s): B2R8T9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 7, 2018
This is version 189 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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