Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cell division coordinator CpoB

Gene

cpoB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division. Promotes physical and functional coordination of the PBP1B-LpoB and Tol machines, and regulates PBP1B activity in response to Tol energy state.1 Publication

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:EG12854-MONOMER

Protein family/group databases

TCDBi2.C.1.2.1 the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division coordinator CpoBUniRule annotationCurated
Gene namesi
Name:cpoB1 PublicationUniRule annotation
Synonyms:ybgF
Ordered Locus Names:b0742, JW0732
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12854 ybgF

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Deletion mutant is sensitive to multiple beta-lactams, including cefsulodin. It leads to defects in outer membrane integrity, envelope defects and elevated cell lysis.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi173Y → D: Decreases self-association. 1 Publication1
Mutagenesisi211P → D: Does not affect oligomerization. 1 Publication1
Mutagenesisi247Y → D: Forms dimers instead of trimers. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000001380427 – 263Cell division coordinator CpoBAdd BLAST237

Proteomic databases

EPDiP45955
PaxDbiP45955
PRIDEiP45955

Interactioni

Subunit structurei

Homotrimer (PubMed:20455268, PubMed:20816983). Interacts directly with the central domain of TolA and with PBP1B (PubMed:11994151, PubMed:20816983, PubMed:25951518). Binding to TolA disrupts the homotrimer to form a YbgF/TolA heterodimer with weak affinity (PubMed:20816983). Forms a quaternary complex with PBP1B-LpoB and TolA (PubMed:25951518).4 Publications

Protein-protein interaction databases

BioGridi4260725, 202 interactors
DIPiDIP-11392N
IntActiP45955, 3 interactors
STRINGi316385.ECDH10B_0809

Structurei

Secondary structure

1263
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP45955
SMRiP45955
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45955

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati143 – 176TPR 11 PublicationAdd BLAST34
Repeati180 – 213TPR 21 PublicationAdd BLAST34
Repeati217 – 250TPR 31 PublicationAdd BLAST34

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili44 – 88UniRule annotationAdd BLAST45

Domaini

Contains an N-terminal coiled-coil domain and a C-terminal TPR domain, separated by a flexible linker. The N-terminal domain forms a stable trimer and controls the oligomeric status. The C-terminal domain, which can also trimerize, is involved in interaction with TolA.2 Publications

Sequence similaritiesi

Belongs to the CpoB family.UniRule annotationCurated

Keywords - Domaini

Coiled coil, Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiENOG4105UFD Bacteria
COG1729 LUCA
HOGENOMiHOG000283059
InParanoidiP45955
OMAiQYWLGEV

Family and domain databases

Gene3Di1.25.40.10, 1 hit
HAMAPiMF_02066 CpoB, 1 hit
InterProiView protein in InterPro
IPR034706 CpoB
IPR014162 CpoB_C
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
IPR032519 YbgF_tri
PfamiView protein in Pfam
PF16331 TolA_bind_tri, 1 hit
PF13174 TPR_6, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit
TIGRFAMsiTIGR02795 tol_pal_ybgF, 1 hit
PROSITEiView protein in PROSITE
PS50293 TPR_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45955-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSNFRHQLL SLSLLVGIAA PWAAFAQAPI SSVGSGSVED RVTQLERISN
60 70 80 90 100
AHSQLLTQLQ QQLSDNQSDI DSLRGQIQEN QYQLNQVVER QKQILLQIDS
110 120 130 140 150
LSSGGAAAQS TSGDQSGAAA STTPTADAGT ANAGAPVKSG NANTDYNAAI
160 170 180 190 200
ALVQDKSRQD DAMVAFQNFI KNYPDSTYLP NANYWLGQLN YNKGKKDDAA
210 220 230 240 250
YYFASVVKNY PKSPKAADAM FKVGVIMQDK GDTAKAKAVY QQVISKYPGT
260
DGAKQAQKRL NAM
Length:263
Mass (Da):28,231
Last modified:November 1, 1997 - v2
Checksum:iEE9845C0A8E803A2
GO

Sequence cautioni

The sequence X65796 differs from that shown. Reason: Frameshift at positions 24 and 41.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti224G → A in X65796 (PubMed:1574003).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65796 Genomic DNA No translation available.
U00096 Genomic DNA Translation: AAC73836.1
AP009048 Genomic DNA Translation: BAA35408.1
PIRiE64810
RefSeqiNP_415270.1, NC_000913.3
WP_000097571.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73836; AAC73836; b0742
BAA35408; BAA35408; BAA35408
GeneIDi947227
KEGGiecj:JW0732
eco:b0742
PATRICifig|1411691.4.peg.1530

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65796 Genomic DNA No translation available.
U00096 Genomic DNA Translation: AAC73836.1
AP009048 Genomic DNA Translation: BAA35408.1
PIRiE64810
RefSeqiNP_415270.1, NC_000913.3
WP_000097571.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WZ7X-ray2.48A/B/C/D/E/F35-109[»]
2XDJX-ray1.82A/B/C/D/E/F35-109[»]
6G5SNMR-A139-263[»]
ProteinModelPortaliP45955
SMRiP45955
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260725, 202 interactors
DIPiDIP-11392N
IntActiP45955, 3 interactors
STRINGi316385.ECDH10B_0809

Protein family/group databases

TCDBi2.C.1.2.1 the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family

Proteomic databases

EPDiP45955
PaxDbiP45955
PRIDEiP45955

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73836; AAC73836; b0742
BAA35408; BAA35408; BAA35408
GeneIDi947227
KEGGiecj:JW0732
eco:b0742
PATRICifig|1411691.4.peg.1530

Organism-specific databases

EchoBASEiEB2698
EcoGeneiEG12854 ybgF

Phylogenomic databases

eggNOGiENOG4105UFD Bacteria
COG1729 LUCA
HOGENOMiHOG000283059
InParanoidiP45955
OMAiQYWLGEV

Enzyme and pathway databases

BioCyciEcoCyc:EG12854-MONOMER

Miscellaneous databases

EvolutionaryTraceiP45955
PROiPR:P45955

Family and domain databases

Gene3Di1.25.40.10, 1 hit
HAMAPiMF_02066 CpoB, 1 hit
InterProiView protein in InterPro
IPR034706 CpoB
IPR014162 CpoB_C
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
IPR032519 YbgF_tri
PfamiView protein in Pfam
PF16331 TolA_bind_tri, 1 hit
PF13174 TPR_6, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit
TIGRFAMsiTIGR02795 tol_pal_ybgF, 1 hit
PROSITEiView protein in PROSITE
PS50293 TPR_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCPOB_ECOLI
AccessioniPrimary (citable) accession number: P45955
Secondary accession number(s): P75756
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: October 10, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again