UniProtKB - P45955 (CPOB_ECOLI)
Protein
Cell division coordinator CpoB
Gene
cpoB
Organism
Escherichia coli (strain K12)
Status
Functioni
Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division. Promotes physical and functional coordination of the PBP1B-LpoB and Tol machines, and regulates PBP1B activity in response to Tol energy state.1 Publication
GO - Biological processi
- cell division Source: EcoCyc
- FtsZ-dependent cytokinesis Source: UniProtKB-UniRule
- protein trimerization Source: InterPro
Keywordsi
Biological process | Cell cycle, Cell division |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12854-MONOMER MetaCyc:EG12854-MONOMER |
Protein family/group databases
TCDBi | 2.C.1.2.1, the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family |
Names & Taxonomyi
Protein namesi | Recommended name: Cell division coordinator CpoBUniRule annotationCurated |
Gene namesi | Name:cpoB1 PublicationUniRule annotation Synonyms:ybgF Ordered Locus Names:b0742, JW0732 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Periplasm UniRule annotation1 Publication Note: Targeting to the Sec-translocase for transport across the inner membrane is SecB-dependent (PubMed:16352602). Localizes to the septum concurrent with PBP1B-LpoB and Tol at the onset of constriction (PubMed:25951518). Localization is dependent on divisome assembly and requires ongoing septal peptidoglycan synthesis (PubMed:25951518).2 Publications
GO - Cellular componenti
- outer membrane-bounded periplasmic space Source: EcoCyc
- protein-containing complex Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Disruption phenotypei
Deletion mutant is sensitive to multiple beta-lactams, including cefsulodin. It leads to defects in outer membrane integrity, envelope defects and elevated cell lysis.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 173 | Y → D: Decreases self-association. 1 Publication | 1 | |
Mutagenesisi | 211 | P → D: Does not affect oligomerization. 1 Publication | 1 | |
Mutagenesisi | 247 | Y → D: Forms dimers instead of trimers. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Sequence analysisAdd BLAST | 26 | |
ChainiPRO_0000013804 | 27 – 263 | Cell division coordinator CpoBAdd BLAST | 237 |
Proteomic databases
jPOSTi | P45955 |
PaxDbi | P45955 |
PRIDEi | P45955 |
Interactioni
Subunit structurei
Homotrimer (PubMed:20455268, PubMed:20816983).
Interacts directly with the central domain of TolA and with PBP1B (PubMed:11994151, PubMed:20816983, PubMed:25951518). Binding to TolA disrupts the homotrimer to form a YbgF/TolA heterodimer with weak affinity (PubMed:20816983).
Forms a quaternary complex with PBP1B-LpoB and TolA (PubMed:25951518).
4 PublicationsProtein-protein interaction databases
BioGRIDi | 4260725, 202 interactors 851559, 2 interactors |
DIPi | DIP-11392N |
IntActi | P45955, 3 interactors |
STRINGi | 511145.b0742 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P45955 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P45955 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 143 – 176 | TPR 11 PublicationAdd BLAST | 34 | |
Repeati | 180 – 213 | TPR 21 PublicationAdd BLAST | 34 | |
Repeati | 217 – 250 | TPR 31 PublicationAdd BLAST | 34 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 44 – 88 | UniRule annotationAdd BLAST | 45 |
Domaini
Contains an N-terminal coiled-coil domain and a C-terminal TPR domain, separated by a flexible linker. The N-terminal domain forms a stable trimer and controls the oligomeric status. The C-terminal domain, which can also trimerize, is involved in interaction with TolA.2 Publications
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Repeat, Signal, TPR repeatPhylogenomic databases
eggNOGi | COG1729, Bacteria |
HOGENOMi | CLU_044315_4_0_6 |
InParanoidi | P45955 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
HAMAPi | MF_02066, CpoB, 1 hit |
InterProi | View protein in InterPro IPR034706, CpoB IPR014162, CpoB_C IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat IPR032519, YbgF_tri |
Pfami | View protein in Pfam PF16331, TolA_bind_tri, 1 hit PF13174, TPR_6, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
TIGRFAMsi | TIGR02795, tol_pal_ybgF, 1 hit |
PROSITEi | View protein in PROSITE PS50293, TPR_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P45955-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSNFRHQLL SLSLLVGIAA PWAAFAQAPI SSVGSGSVED RVTQLERISN
60 70 80 90 100
AHSQLLTQLQ QQLSDNQSDI DSLRGQIQEN QYQLNQVVER QKQILLQIDS
110 120 130 140 150
LSSGGAAAQS TSGDQSGAAA STTPTADAGT ANAGAPVKSG NANTDYNAAI
160 170 180 190 200
ALVQDKSRQD DAMVAFQNFI KNYPDSTYLP NANYWLGQLN YNKGKKDDAA
210 220 230 240 250
YYFASVVKNY PKSPKAADAM FKVGVIMQDK GDTAKAKAVY QQVISKYPGT
260
DGAKQAQKRL NAM
Sequence cautioni
The sequence X65796 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 224 | G → A in X65796 (PubMed:1574003).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65796 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC73836.1 AP009048 Genomic DNA Translation: BAA35408.1 |
PIRi | E64810 |
RefSeqi | NP_415270.1, NC_000913.3 WP_000097571.1, NZ_SSZK01000033.1 |
Genome annotation databases
EnsemblBacteriai | AAC73836; AAC73836; b0742 BAA35408; BAA35408; BAA35408 |
GeneIDi | 947227 |
KEGGi | ecj:JW0732 eco:b0742 |
PATRICi | fig|1411691.4.peg.1530 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X65796 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC73836.1 AP009048 Genomic DNA Translation: BAA35408.1 |
PIRi | E64810 |
RefSeqi | NP_415270.1, NC_000913.3 WP_000097571.1, NZ_SSZK01000033.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2WZ7 | X-ray | 2.48 | A/B/C/D/E/F | 35-109 | [»] | |
2XDJ | X-ray | 1.82 | A/B/C/D/E/F | 35-109 | [»] | |
6G5S | NMR | - | A | 139-263 | [»] | |
SMRi | P45955 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260725, 202 interactors 851559, 2 interactors |
DIPi | DIP-11392N |
IntActi | P45955, 3 interactors |
STRINGi | 511145.b0742 |
Protein family/group databases
TCDBi | 2.C.1.2.1, the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family |
Proteomic databases
jPOSTi | P45955 |
PaxDbi | P45955 |
PRIDEi | P45955 |
Genome annotation databases
EnsemblBacteriai | AAC73836; AAC73836; b0742 BAA35408; BAA35408; BAA35408 |
GeneIDi | 947227 |
KEGGi | ecj:JW0732 eco:b0742 |
PATRICi | fig|1411691.4.peg.1530 |
Organism-specific databases
EchoBASEi | EB2698 |
Phylogenomic databases
eggNOGi | COG1729, Bacteria |
HOGENOMi | CLU_044315_4_0_6 |
InParanoidi | P45955 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12854-MONOMER MetaCyc:EG12854-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P45955 |
PROi | PR:P45955 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
HAMAPi | MF_02066, CpoB, 1 hit |
InterProi | View protein in InterPro IPR034706, CpoB IPR014162, CpoB_C IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat IPR032519, YbgF_tri |
Pfami | View protein in Pfam PF16331, TolA_bind_tri, 1 hit PF13174, TPR_6, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
TIGRFAMsi | TIGR02795, tol_pal_ybgF, 1 hit |
PROSITEi | View protein in PROSITE PS50293, TPR_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CPOB_ECOLI | |
Accessioni | P45955Primary (citable) accession number: P45955 Secondary accession number(s): P75756 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families