UniProtKB - P45955 (CPOB_ECOLI)

BLAST

>sp|P45955|CPOB_ECOLI Cell division coordinator CpoB OS=Escherichia coli (strain K12) OX=83333 GN=cpoB PE=1 SV=2
MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQLERISNAHSQLLTQLQ
QQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLSSGGAAAQSTSGDQSGAAA
STTPTADAGTANAGAPVKSGNANTDYNAAIALVQDKSRQDDAMVAFQNFIKNYPDSTYLP
NANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAADAMFKVGVIMQDKGDTAKAKAVY
QQVISKYPGTDGAKQAQKRLNAM

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History
Entry version 142 (10 Oct 2018)
Sequence version 2 (01 Nov 1997)
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Protein

Cell division coordinator CpoB

Gene

cpoB

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division. Promotes physical and functional coordination of the PBP1B-LpoB and Tol machines, and regulates PBP1B activity in response to Tol energy state.1 Publication

GO - Biological processⁱ

cell division
Source: EcoCyc
Inferred from direct assayⁱ
- 25951518
FtsZ-dependent cytokinesis Source: UniProtKB-UniRule
protein homooligomerization
Source: EcoCyc
Inferred from direct assayⁱ
- 20455268
protein trimerization Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cell cycle, Cell division

Biological process

Cell cycle, Cell division

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG12854-MONOMER

BioCycⁱ

EcoCyc:EG12854-MONOMER

Protein family/group databases

TCDBⁱ	2.C.1.2.1 the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family

TCDBⁱ

2.C.1.2.1 the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Cell division coordinator CpoBUniRule annotationCurated
Gene namesⁱ	Name:cpoB1 PublicationUniRule annotation Synonyms:ybgF Ordered Locus Names:b0742, JW0732
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG12854 ybgF

EcoGeneⁱ

EG12854 ybgF

Subcellular locationⁱ

Periplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_02066
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "Characterization of the tol-pal region of Escherichia coli K-12: translational control of tolR expression by TolQ and identification of a new open reading frame downstream of pal encoding a periplasmic protein."
  Vianney A., Muller M.M., Clavel T., Lazzaroni J.C., Portalier R., Webster R.E.
  J. Bacteriol. 178:4031-4038(1996) [PubMed] [Europe PMC] [Abstract]
  Cited for: IDENTIFICATION, SUBCELLULAR LOCATION.

Note:

Targeting to the Sec-translocase for transport across the inner membrane is SecB-dependent (PubMed:16352602). Localizes to the septum concurrent with PBP1B-LpoB and Tol at the onset of constriction (PubMed:25951518). Localization is dependent on divisome assembly and requires ongoing septal peptidoglycan synthesis (PubMed:25951518).2 Publications

GO - Cellular componentⁱ

outer membrane-bounded periplasmic space
Source: EcoCyc
Inferred from direct assayⁱ
- 8763928

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Periplasm

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	173	Y → D: Decreases self-association. 1 Publication	1
Mutagenesisⁱ	211	P → D: Does not affect oligomerization. 1 Publication	1
Mutagenesisⁱ	247	Y → D: Forms dimers instead of trimers. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 26	Sequence analysisAdd BLAST		26
ChainⁱPRO_0000013804	27 – 263	Cell division coordinator CpoBAdd BLAST		237

Proteomic databases

EPDⁱ	P45955
PaxDbⁱ	P45955
PRIDEⁱ	P45955

Interactionⁱ

Subunit structureⁱ

Homotrimer (PubMed:20455268, PubMed:20816983). Interacts directly with the central domain of TolA and with PBP1B (PubMed:11994151, PubMed:20816983, PubMed:25951518). Binding to TolA disrupts the homotrimer to form a YbgF/TolA heterodimer with weak affinity (PubMed:20816983). Forms a quaternary complex with PBP1B-LpoB and TolA (PubMed:25951518).4 Publications

Protein-protein interaction databases

BioGridⁱ	4260725, 202 interactors
Database of interacting proteins More...DIPⁱ	DIP-11392N
IntActⁱ	P45955, 3 interactors
STRINGⁱ	316385.ECDH10B_0809

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Helixⁱ	38 – 99	Combined sources		62

Show more details

3D structure databases

ProteinModelPortalⁱ	P45955
SMRⁱ	P45955
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P45955

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Repeatⁱ	143 – 176	TPR 11 PublicationAdd BLAST	34
Repeatⁱ	180 – 213	TPR 21 PublicationAdd BLAST	34
Repeatⁱ	217 – 250	TPR 31 PublicationAdd BLAST	34

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	44 – 88	UniRule annotationAdd BLAST		45

Domainⁱ

Contains an N-terminal coiled-coil domain and a C-terminal TPR domain, separated by a flexible linker. The N-terminal domain forms a stable trimer and controls the oligomeric status. The C-terminal domain, which can also trimerize, is involved in interaction with TolA.2 Publications

Sequence similaritiesⁱ

Belongs to the CpoB family.UniRule annotationCurated

Keywords - Domainⁱ

Coiled coil, Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGⁱ	ENOG4105UFD Bacteria COG1729 LUCA
HOGENOMⁱ	HOG000283059
InParanoidⁱ	P45955
OMAⁱ	QYWLGEV

Family and domain databases

Gene3Dⁱ	1.25.40.10, 1 hit
HAMAPⁱ	MF_02066 CpoB, 1 hit
InterProⁱ	View protein in InterPro IPR034706 CpoB IPR014162 CpoB_C IPR013026 TPR-contain_dom IPR011990 TPR-like_helical_dom_sf IPR019734 TPR_repeat IPR032519 YbgF_tri
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF16331 TolA_bind_tri, 1 hit PF13174 TPR_6, 1 hit
SUPFAMⁱ	SSF48452 SSF48452, 1 hit
TIGRFAMsⁱ	TIGR02795 tol_pal_ybgF, 1 hit
PROSITEⁱ	View protein in PROSITE PS50293 TPR_REGION, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P45955-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSSNFRHQLL SLSLLVGIAA PWAAFAQAPI SSVGSGSVED RVTQLERISN 
        60         70         80         90        100
AHSQLLTQLQ QQLSDNQSDI DSLRGQIQEN QYQLNQVVER QKQILLQIDS 
       110        120        130        140        150
LSSGGAAAQS TSGDQSGAAA STTPTADAGT ANAGAPVKSG NANTDYNAAI 
       160        170        180        190        200
ALVQDKSRQD DAMVAFQNFI KNYPDSTYLP NANYWLGQLN YNKGKKDDAA 
       210        220        230        240        250
YYFASVVKNY PKSPKAADAM FKVGVIMQDK GDTAKAKAVY QQVISKYPGT 
       260 
DGAKQAQKRL NAM

Length:263

Mass (Da):28,231

Last modified:November 1, 1997 - v2

Checksum:ⁱEE9845C0A8E803A2

Sequence cautionⁱ

The sequence X65796 differs from that shown. Reason: Frameshift at positions 24 and 41.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	224	G → A in X65796 (PubMed:1574003).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X65796 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC73836.1 AP009048 Genomic DNA Translation: BAA35408.1
PIRⁱ	E64810
NCBI Reference Sequences More...RefSeqⁱ	NP_415270.1, NC_000913.3 WP_000097571.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73836; AAC73836; b0742 BAA35408; BAA35408; BAA35408
GeneIDⁱ	947227
KEGGⁱ	ecj:JW0732 eco:b0742
PATRICⁱ	fig\|1411691.4.peg.1530

Protein	Similar proteins		Species	Score	Length	Source
P45955	Cell division coordinator CpoB		ECOLX		263	UniRef100_P45955
Cell division coordinator CpoB		Escherichia coli 1-110-08_S3_C1		263
Cell division coordinator CpoB		Escherichia coli 2-210-07_S3_C3		263
Cell division coordinator CpoB		Escherichia coli DEC6A		263
Cell division coordinator CpoB		Escherichia coli H386		263
+9

Protein	Similar proteins		Species	Score	Length	Source
P45955	Cell division coordinator CpoB		ECOLX		263	UniRef90_P45955
Cell division coordinator CpoB		Escherichia coli 1-110-08_S3_C1		263
Cell division coordinator CpoB		Escherichia coli 2-210-07_S3_C3		263
Cell division coordinator CpoB		Escherichia coli DEC6A		263
Cell division coordinator CpoB		Escherichia coli H386		263
+566

Protein	Similar proteins		Species	Score	Length	Source
P45955	Cell division coordinator CpoB		ECOLX		263	UniRef50_P45955
Cell division coordinator CpoB		Escherichia coli 1-110-08_S3_C1		263
Cell division coordinator CpoB		Escherichia coli 2-210-07_S3_C3		263
Cell division coordinator CpoB		Escherichia coli DEC6A		263
Cell division coordinator CpoB		Escherichia coli H386		263
+1687

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X65796 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC73836.1 AP009048 Genomic DNA Translation: BAA35408.1
PIRⁱ	E64810
RefSeqⁱ	NP_415270.1, NC_000913.3 WP_000097571.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2WZ7	X-ray	2.48	A/B/C/D/E/F	35-109	[»]
	2XDJ	X-ray	1.82	A/B/C/D/E/F	35-109	[»]
	6G5S	NMR	-	A	139-263	[»]
ProteinModelPortalⁱ	P45955
SMRⁱ	P45955
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4260725, 202 interactors
DIPⁱ	DIP-11392N
IntActⁱ	P45955, 3 interactors
STRINGⁱ	316385.ECDH10B_0809

Protein family/group databases

TCDBⁱ	2.C.1.2.1 the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family

TCDBⁱ

2.C.1.2.1 the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family

Proteomic databases

EPDⁱ	P45955
PaxDbⁱ	P45955
PRIDEⁱ	P45955

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73836; AAC73836; b0742 BAA35408; BAA35408; BAA35408
GeneIDⁱ	947227
KEGGⁱ	ecj:JW0732 eco:b0742
PATRICⁱ	fig\|1411691.4.peg.1530

Organism-specific databases

EchoBASEⁱ	EB2698
EcoGeneⁱ	EG12854 ybgF

Phylogenomic databases

eggNOGⁱ	ENOG4105UFD Bacteria COG1729 LUCA
HOGENOMⁱ	HOG000283059
InParanoidⁱ	P45955
OMAⁱ	QYWLGEV

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG12854-MONOMER

BioCycⁱ

EcoCyc:EG12854-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	P45955
Protein Ontology More...PROⁱ	PR:P45955

Family and domain databases

Gene3Dⁱ	1.25.40.10, 1 hit
HAMAPⁱ	MF_02066 CpoB, 1 hit
InterProⁱ	View protein in InterPro IPR034706 CpoB IPR014162 CpoB_C IPR013026 TPR-contain_dom IPR011990 TPR-like_helical_dom_sf IPR019734 TPR_repeat IPR032519 YbgF_tri
Pfamⁱ	View protein in Pfam PF16331 TolA_bind_tri, 1 hit PF13174 TPR_6, 1 hit
SUPFAMⁱ	SSF48452 SSF48452, 1 hit
TIGRFAMsⁱ	TIGR02795 tol_pal_ybgF, 1 hit
PROSITEⁱ	View protein in PROSITE PS50293 TPR_REGION, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CPOB_ECOLI
Accessionⁱ	P45955Primary (citable) accession number: P45955 Secondary accession number(s): P75756
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
	Last sequence update:	November 1, 1997
	Last modified:	October 10, 2018
	This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

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Supporting data

UniProtKB - P45955 (CPOB_ECOLI)

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Cell division coordinator CpoB

cpoB

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ