UniProtKB - P45880 (VDAC2_HUMAN)
Protein
Voltage-dependent anion-selective channel protein 2
Gene
VDAC2
Organism
Homo sapiens (Human)
Status
Functioni
Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules (By similarity). The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (By similarity). The open state has a weak anion selectivity whereas the closed state is cation-selective (By similarity). Binds various lipids, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterol cholesterol (PubMed:31015432). Binding of ceramide promotes the mitochondrial outer membrane permeabilization (MOMP) apoptotic pathway (PubMed:31015432).By similarity1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 84 | Involved in ceramide and phosphatidylcholine binding1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 253 – 255 | NADBy similarity | 3 | |
Nucleotide bindingi | 271 – 275 | NADBy similarity | 5 |
GO - Molecular functioni
- ceramide binding Source: UniProtKB
- cholesterol binding Source: UniProtKB
- nucleotide binding Source: UniProtKB-KW
- phosphatidylcholine binding Source: UniProtKB
- porin activity Source: UniProtKB-KW
- voltage-gated anion channel activity Source: UniProtKB
GO - Biological processi
- anion transport Source: UniProtKB
- binding of sperm to zona pellucida Source: CAFA
- mitochondrial outer membrane permeabilization Source: UniProtKB
- negative regulation of intrinsic apoptotic signaling pathway Source: Ensembl
- negative regulation of protein polymerization Source: Ensembl
Keywordsi
Molecular function | Porin |
Biological process | Ion transport, Transport |
Ligand | Lipid-binding, NAD, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P45880 |
Reactomei | R-HSA-5689880, Ub-specific processing proteases R-HSA-8949215, Mitochondrial calcium ion transport |
Protein family/group databases
MoonProti | P45880 |
TCDBi | 1.B.8.1.12, the mitochondrial and plastid porin (mpp) family |
Names & Taxonomyi
Protein namesi | Recommended name: Voltage-dependent anion-selective channel protein 2Short name: VDAC-2 Short name: hVDAC2 Alternative name(s): Outer mitochondrial membrane protein porin 2 |
Gene namesi | Name:VDAC2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000165637.13 |
HGNCi | HGNC:12672, VDAC2 |
MIMi | 193245, gene |
neXtProti | NX_P45880 |
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane 2 Publications
Other locations
- Membrane 1 Publication
Note: May localize to non-mitochondrial membranes.1 Publication
Mitochondrion
- mitochondrial membrane Source: UniProtKB
- mitochondrial nucleoid Source: BHF-UCL
- mitochondrial outer membrane Source: GO_Central
- mitochondrion Source: HPA
Nucleus
- nucleus Source: UniProtKB
Other locations
- acrosomal vesicle Source: CAFA
- membrane Source: UniProtKB
- membrane raft Source: UniProtKB
- pore complex Source: UniProtKB-KW
- synaptic vesicle Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 37 – 46 | Beta strandedBy similarity | 10 | |
Transmembranei | 50 – 58 | Beta strandedBy similarity | 9 | |
Transmembranei | 65 – 75 | Beta strandedBy similarityAdd BLAST | 11 | |
Transmembranei | 80 – 87 | Beta strandedBy similarity | 8 | |
Transmembranei | 91 – 100 | Beta strandedBy similarity | 10 | |
Transmembranei | 106 – 115 | Beta strandedBy similarity | 10 | |
Transmembranei | 122 – 131 | Beta strandedBy similarity | 10 | |
Transmembranei | 134 – 141 | Beta strandedBy similarity | 8 | |
Transmembranei | 148 – 156 | Beta strandedBy similarity | 9 | |
Transmembranei | 161 – 169 | Beta strandedBy similarity | 9 | |
Transmembranei | 174 – 186 | Beta strandedBy similarityAdd BLAST | 13 | |
Transmembranei | 189 – 196 | Beta strandedBy similarity | 8 | |
Transmembranei | 200 – 209 | Beta strandedBy similarity | 10 | |
Transmembranei | 213 – 222 | Beta strandedBy similarity | 10 | |
Transmembranei | 229 – 238 | Beta strandedBy similarity | 10 | |
Transmembranei | 242 – 249 | Beta strandedBy similarity | 8 | |
Transmembranei | 253 – 262 | Beta strandedBy similarity | 10 | |
Transmembranei | 265 – 274 | Beta strandedBy similarity | 10 | |
Transmembranei | 284 – 293 | Beta strandedBy similarity | 10 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 84 | E → Q: Abolishes ceramide and phosphatidylcholine binding. Decreases apoptosis frequency following mitochondrial targeting of ceramide. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 7417 |
OpenTargetsi | ENSG00000165637 |
PharmGKBi | PA37295 |
Miscellaneous databases
Pharosi | P45880, Tchem |
Chemistry databases
ChEMBLi | CHEMBL6190 |
DrugBanki | DB01375, Aluminium monostearate DB06098, PRLX 93936 |
Polymorphism and mutation databases
BioMutai | VDAC2 |
DMDMi | 158518391 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000050505 | 2 – 294 | Voltage-dependent anion-selective channel protein 2Add BLAST | 293 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 31 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 31 | N6-succinyllysine; alternateBy similarity | 1 | |
Cross-linki | 31 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate1 Publication | ||
Cross-linki | 64 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 72 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 78 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 118 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 120 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 120 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate1 Publication | ||
Cross-linki | 121 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 124 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 172 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 251 | PhosphoserineBy similarity | 1 | |
Modified residuei | 277 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 277 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate1 Publication | ||
Cross-linki | 285 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication |
Post-translational modificationi
Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.1 Publication
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P45880 |
jPOSTi | P45880 |
MassIVEi | P45880 |
PaxDbi | P45880 |
PeptideAtlasi | P45880 |
PRIDEi | P45880 |
ProteomicsDBi | 55687 [P45880-3] 55688 [P45880-1] 55689 [P45880-2] |
TopDownProteomicsi | P45880-3 [P45880-3] |
2D gel databases
DOSAC-COBS-2DPAGEi | P45880 |
OGPi | P45880 |
REPRODUCTION-2DPAGEi | P45880 |
SWISS-2DPAGEi | P45880 |
UCD-2DPAGEi | P45880 |
PTM databases
CarbonylDBi | P45880 |
iPTMneti | P45880 |
PhosphoSitePlusi | P45880 |
SwissPalmi | P45880 |
Expressioni
Tissue specificityi
Expressed in erythrocytes (at protein level) (PubMed:27641616). Expressed in all tissues examined (PubMed:8420959).2 Publications
Gene expression databases
Bgeei | ENSG00000165637, Expressed in ectocervix and 132 other tissues |
ExpressionAtlasi | P45880, baseline and differential |
Genevisiblei | P45880, HS |
Organism-specific databases
HPAi | ENSG00000165637, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with hexokinases (By similarity).
By similarityBinary interactionsi
Hide detailsP45880
With | #Exp. | IntAct |
---|---|---|
FAM162A [Q96A26] | 2 | EBI-354022,EBI-6123466 |
VDAC1 [P21796] | 5 | EBI-354022,EBI-354158 |
Isoform 3 [P45880-3]
With | #Exp. | IntAct |
---|---|---|
ZP2 [Q05996] | 2 | EBI-11614013,EBI-1755919 |
ZP3 [P21754] | 2 | EBI-11614013,EBI-11783624 |
Protein-protein interaction databases
BioGRIDi | 113260, 283 interactors |
CORUMi | P45880 |
IntActi | P45880, 90 interactors |
MINTi | P45880 |
STRINGi | 9606.ENSP00000361635 |
Chemistry databases
BindingDBi | P45880 |
Miscellaneous databases
RNActi | P45880, protein |
Family & Domainsi
Domaini
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.By similarity
Sequence similaritiesi
Belongs to the eukaryotic mitochondrial porin family.Curated
Keywords - Domaini
Transmembrane, Transmembrane beta strandPhylogenomic databases
eggNOGi | KOG3126, Eukaryota |
GeneTreei | ENSGT00950000182869 |
HOGENOMi | CLU_044399_2_0_1 |
InParanoidi | P45880 |
OMAi | WDSKAGN |
OrthoDBi | 938262at2759 |
PhylomeDBi | P45880 |
TreeFami | TF315091 |
Family and domain databases
Gene3Di | 2.40.160.10, 1 hit |
InterProi | View protein in InterPro IPR023614, Porin_dom_sf IPR001925, Porin_Euk IPR027246, Porin_Euk/Tom40 IPR030277, VDAC2 |
PANTHERi | PTHR11743, PTHR11743, 1 hit PTHR11743:SF12, PTHR11743:SF12, 1 hit |
Pfami | View protein in Pfam PF01459, Porin_3, 1 hit |
PRINTSi | PR00185, EUKARYTPORIN |
PROSITEi | View protein in PROSITE PS00558, EUKARYOTIC_PORIN, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 3 (identifier: P45880-3) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATHGQTCAR PMCIPPSYAD LGKAARDIFN KGFGFGLVKL DVKTKSCSGV
60 70 80 90 100
EFSTSGSSNT DTGKVTGTLE TKYKWCEYGL TFTEKWNTDN TLGTEIAIED
110 120 130 140 150
QICQGLKLTF DTTFSPNTGK KSGKIKSSYK RECINLGCDV DFDFAGPAIH
160 170 180 190 200
GSAVFGYEGW LAGYQMTFDS AKSKLTRNNF AVGYRTGDFQ LHTNVNDGTE
210 220 230 240 250
FGGSIYQKVC EDLDTSVNLA WTSGTNCTRF GIAAKYQLDP TASISAKVNN
260 270 280 290
SSLIGVGYTQ TLRPGVKLTL SALVDGKSIN AGGHKVGLAL ELEA
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5JSD1 | Q5JSD1_HUMAN | Outer mitochondrial membrane protei... | VDAC2 | 204 | Annotation score: | ||
Q5JSD2 | Q5JSD2_HUMAN | Outer mitochondrial membrane protei... | VDAC2 | 194 | Annotation score: | ||
A0A0A0MR02 | A0A0A0MR02_HUMAN | Outer mitochondrial membrane protei... | VDAC2 | 282 | Annotation score: | ||
A2A3S1 | A2A3S1_HUMAN | Outer mitochondrial membrane protei... | VDAC2 | 109 | Annotation score: |
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_006380 | 24 | A → V1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005077 | 1 – 11 | MATHGQTCARP → MSWCNELRLPALKQHSIGRG LESHIT in isoform 1. 1 PublicationAdd BLAST | 11 | |
Alternative sequenceiVSP_005076 | 1 – 11 | Missing in isoform 2. 2 PublicationsAdd BLAST | 11 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L08666 mRNA Translation: AAA60144.1 Frameshift. L08666 mRNA Translation: AAA60145.1 Frameshift. L06328 mRNA Translation: AAB59457.1 AF152227 , AF152220, AF152221, AF152222, AF152223, AF152224, AF152225, AF152226 Genomic DNA Translation: AAD40241.1 CR456964 mRNA Translation: CAG33245.1 AL390034 Genomic DNA No translation available. AL392111 Genomic DNA No translation available. BC000165 mRNA Translation: AAH00165.2 BC012883 mRNA Translation: AAH12883.2 BC072407 mRNA Translation: AAH72407.1 |
CCDSi | CCDS53544.1 [P45880-1] CCDS7348.1 [P45880-3] |
PIRi | A45972 B44422 |
RefSeqi | NP_001171712.1, NM_001184783.2 [P45880-1] NP_001171752.1, NM_001184823.1 [P45880-3] NP_001311017.1, NM_001324088.1 [P45880-3] NP_003366.2, NM_003375.4 [P45880-3] |
Genome annotation databases
Ensembli | ENST00000313132; ENSP00000361635; ENSG00000165637 [P45880-1] ENST00000332211; ENSP00000361686; ENSG00000165637 [P45880-3] ENST00000543351; ENSP00000443092; ENSG00000165637 [P45880-3] |
GeneIDi | 7417 |
KEGGi | hsa:7417 |
UCSCi | uc001jwz.5, human [P45880-3] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L08666 mRNA Translation: AAA60144.1 Frameshift. L08666 mRNA Translation: AAA60145.1 Frameshift. L06328 mRNA Translation: AAB59457.1 AF152227 , AF152220, AF152221, AF152222, AF152223, AF152224, AF152225, AF152226 Genomic DNA Translation: AAD40241.1 CR456964 mRNA Translation: CAG33245.1 AL390034 Genomic DNA No translation available. AL392111 Genomic DNA No translation available. BC000165 mRNA Translation: AAH00165.2 BC012883 mRNA Translation: AAH12883.2 BC072407 mRNA Translation: AAH72407.1 |
CCDSi | CCDS53544.1 [P45880-1] CCDS7348.1 [P45880-3] |
PIRi | A45972 B44422 |
RefSeqi | NP_001171712.1, NM_001184783.2 [P45880-1] NP_001171752.1, NM_001184823.1 [P45880-3] NP_001311017.1, NM_001324088.1 [P45880-3] NP_003366.2, NM_003375.4 [P45880-3] |
3D structure databases
SMRi | P45880 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 113260, 283 interactors |
CORUMi | P45880 |
IntActi | P45880, 90 interactors |
MINTi | P45880 |
STRINGi | 9606.ENSP00000361635 |
Chemistry databases
BindingDBi | P45880 |
ChEMBLi | CHEMBL6190 |
DrugBanki | DB01375, Aluminium monostearate DB06098, PRLX 93936 |
Protein family/group databases
MoonProti | P45880 |
TCDBi | 1.B.8.1.12, the mitochondrial and plastid porin (mpp) family |
PTM databases
CarbonylDBi | P45880 |
iPTMneti | P45880 |
PhosphoSitePlusi | P45880 |
SwissPalmi | P45880 |
Polymorphism and mutation databases
BioMutai | VDAC2 |
DMDMi | 158518391 |
2D gel databases
DOSAC-COBS-2DPAGEi | P45880 |
OGPi | P45880 |
REPRODUCTION-2DPAGEi | P45880 |
SWISS-2DPAGEi | P45880 |
UCD-2DPAGEi | P45880 |
Proteomic databases
EPDi | P45880 |
jPOSTi | P45880 |
MassIVEi | P45880 |
PaxDbi | P45880 |
PeptideAtlasi | P45880 |
PRIDEi | P45880 |
ProteomicsDBi | 55687 [P45880-3] 55688 [P45880-1] 55689 [P45880-2] |
TopDownProteomicsi | P45880-3 [P45880-3] |
Protocols and materials databases
Antibodypediai | 29670, 281 antibodies |
DNASUi | 7417 |
Genome annotation databases
Ensembli | ENST00000313132; ENSP00000361635; ENSG00000165637 [P45880-1] ENST00000332211; ENSP00000361686; ENSG00000165637 [P45880-3] ENST00000543351; ENSP00000443092; ENSG00000165637 [P45880-3] |
GeneIDi | 7417 |
KEGGi | hsa:7417 |
UCSCi | uc001jwz.5, human [P45880-3] |
Organism-specific databases
CTDi | 7417 |
DisGeNETi | 7417 |
EuPathDBi | HostDB:ENSG00000165637.13 |
GeneCardsi | VDAC2 |
HGNCi | HGNC:12672, VDAC2 |
HPAi | ENSG00000165637, Low tissue specificity |
MIMi | 193245, gene |
neXtProti | NX_P45880 |
OpenTargetsi | ENSG00000165637 |
PharmGKBi | PA37295 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3126, Eukaryota |
GeneTreei | ENSGT00950000182869 |
HOGENOMi | CLU_044399_2_0_1 |
InParanoidi | P45880 |
OMAi | WDSKAGN |
OrthoDBi | 938262at2759 |
PhylomeDBi | P45880 |
TreeFami | TF315091 |
Enzyme and pathway databases
PathwayCommonsi | P45880 |
Reactomei | R-HSA-5689880, Ub-specific processing proteases R-HSA-8949215, Mitochondrial calcium ion transport |
Miscellaneous databases
BioGRID-ORCSi | 7417, 112 hits in 845 CRISPR screens |
ChiTaRSi | VDAC2, human |
GeneWikii | VDAC2 |
GenomeRNAii | 7417 |
Pharosi | P45880, Tchem |
PROi | PR:P45880 |
RNActi | P45880, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000165637, Expressed in ectocervix and 132 other tissues |
ExpressionAtlasi | P45880, baseline and differential |
Genevisiblei | P45880, HS |
Family and domain databases
Gene3Di | 2.40.160.10, 1 hit |
InterProi | View protein in InterPro IPR023614, Porin_dom_sf IPR001925, Porin_Euk IPR027246, Porin_Euk/Tom40 IPR030277, VDAC2 |
PANTHERi | PTHR11743, PTHR11743, 1 hit PTHR11743:SF12, PTHR11743:SF12, 1 hit |
Pfami | View protein in Pfam PF01459, Porin_3, 1 hit |
PRINTSi | PR00185, EUKARYTPORIN |
PROSITEi | View protein in PROSITE PS00558, EUKARYOTIC_PORIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VDAC2_HUMAN | |
Accessioni | P45880Primary (citable) accession number: P45880 Secondary accession number(s): Q5VWK1 Q9Y5I6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | September 11, 2007 | |
Last modified: | December 2, 2020 | |
This is version 199 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations