UniProtKB - P45858 (MMGD_BACSU)
Protein
Citrate/2-methylcitrate synthase
Gene
mmgD
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Involved in both the tricarboxylic acid (TCA) and methylcitric acid cycles (PubMed:28956599). Has both 2-methylcitrate synthase and citrate synthase activities. Catalyzes the condensation of propionyl-CoA and oxaloacetate to yield 2-methylcitrate (2-MC) and CoA, and the condensation of acetyl-CoA and oxaloacetate to yield citrate and CoA (PubMed:8759838, PubMed:28956599). Has 2.3-fold higher activity as a 2-methylcitrate synthase (PubMed:28956599). Catalyzes the formation of either (2S,3R)- or (2R,3S)-2-methylcitrate (PubMed:28956599).2 Publications
Miscellaneous
Bifunctionality as a citrate and 2-methylcitrate synthase is likely important in the mother cell's physiological milieu.1 Publication
Catalytic activityi
- EC:2.3.3.161 Publication
: tricarboxylic acid cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.1 PublicationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Citrate synthase 2 (citZ), Citrate synthase 1 (citA), Citrate/2-methylcitrate synthase (mmgD)
- Aconitate hydratase (acnA), Aconitate/2-methylaconitate hydratase (citB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 188 | SubstrateBy similarity | 1 | |
Active sitei | 223 | By similarity | 1 | |
Active sitei | 262 | By similarity | 1 | |
Binding sitei | 272 | SubstrateBy similarity | 1 | |
Active sitei | 314 | By similarity | 1 | |
Binding sitei | 339 | SubstrateBy similarity | 1 | |
Binding sitei | 358 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- 2-methylcitrate synthase activity Source: UniProtKB
- citrate (Si)-synthase activity Source: GO_Central
- citrate synthase activity Source: UniProtKB
GO - Biological processi
- carbohydrate metabolic process Source: GO_Central
- propionate metabolic process, methylcitrate cycle Source: UniProtKB
- sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
- tricarboxylic acid cycle Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Sporulation, Tricarboxylic acid cycle |
Enzyme and pathway databases
BioCyci | BSUB:BSU24140-MONOMER |
UniPathwayi | UPA00223;UER00717 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mmgD1 Publication Synonyms:yqiO Ordered Locus Names:BSU24140 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169930 | 1 – 372 | Citrate/2-methylcitrate synthaseAdd BLAST | 372 |
Proteomic databases
PaxDbi | P45858 |
PRIDEi | P45858 |
Expressioni
Developmental stagei
Expressed in the mother cell at intermediate stages of sporulation under the control of the sigma-E factor.1 Publication
Inductioni
Subject to catabolite repression.1 Publication
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 256 – 260 | Coenzyme A bindingBy similarity | 5 |
Sequence similaritiesi
Belongs to the citrate synthase family.Curated
Phylogenomic databases
eggNOGi | COG0372, Bacteria |
InParanoidi | P45858 |
OMAi | NEAVMHM |
PhylomeDBi | P45858 |
Family and domain databases
Gene3Di | 1.10.230.10, 1 hit 1.10.580.10, 1 hit |
InterProi | View protein in InterPro IPR016142, Citrate_synth-like_lrg_a-sub IPR016143, Citrate_synth-like_sm_a-sub IPR002020, Citrate_synthase IPR019810, Citrate_synthase_AS IPR024176, Citrate_synthase_bac-typ IPR036969, Citrate_synthase_sf |
PANTHERi | PTHR11739, PTHR11739, 1 hit |
Pfami | View protein in Pfam PF00285, Citrate_synt, 1 hit |
PIRSFi | PIRSF001369, Citrate_synth, 1 hit |
PRINTSi | PR00143, CITRTSNTHASE |
SUPFAMi | SSF48256, SSF48256, 1 hit |
PROSITEi | View protein in PROSITE PS00480, CITRATE_SYNTHASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P45858-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEEKQHYSPG LDGVIAAETH ISYLDTQSSQ ILIRGYDLIE LSETKSYLEL
60 70 80 90 100
VHLLLEGRLP EESEMETLER KINSASSLPA DHLRLLELLP EDTHPMDGLR
110 120 130 140 150
TGLSALAGYD RQIDDRSPSA NKERAYQLLG KMPALTAASY RIINKKEPIL
160 170 180 190 200
PLQTLSYSAN FLYMMTGKLP SSLEEQIFDR SLVLYSEHEM PNSTFAARVI
210 220 230 240 250
ASTHSDLYGA LTGAVASLKG NLHGGANEAV MYLLLEAKTT SDFEQLLQTK
260 270 280 290 300
LKRKEKIMGF GHRVYMKKMD PRALMMKEAL QQLCDKAGDH RLYEMCEAGE
310 320 330 340 350
RLMEKEKGLY PNLDYYAAPV YWMLGIPIPL YTPIFFSART SGLCAHVIEQ
360 370
HANNRLFRPR VSYMGPRYQT KS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U29084 Genomic DNA Translation: AAB09616.1 D84432 Genomic DNA Translation: BAA12590.1 AL009126 Genomic DNA Translation: CAB14345.1 |
PIRi | E69658 |
RefSeqi | NP_390294.1, NC_000964.3 WP_003230298.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14345; CAB14345; BSU24140 |
GeneIDi | 938665 |
KEGGi | bsu:BSU24140 |
PATRICi | fig|224308.179.peg.2628 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U29084 Genomic DNA Translation: AAB09616.1 D84432 Genomic DNA Translation: BAA12590.1 AL009126 Genomic DNA Translation: CAB14345.1 |
PIRi | E69658 |
RefSeqi | NP_390294.1, NC_000964.3 WP_003230298.1, NZ_JNCM01000036.1 |
3D structure databases
SMRi | P45858 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU24140 |
Proteomic databases
PaxDbi | P45858 |
PRIDEi | P45858 |
Genome annotation databases
EnsemblBacteriai | CAB14345; CAB14345; BSU24140 |
GeneIDi | 938665 |
KEGGi | bsu:BSU24140 |
PATRICi | fig|224308.179.peg.2628 |
Phylogenomic databases
eggNOGi | COG0372, Bacteria |
InParanoidi | P45858 |
OMAi | NEAVMHM |
PhylomeDBi | P45858 |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER00717 |
BioCyci | BSUB:BSU24140-MONOMER |
Family and domain databases
Gene3Di | 1.10.230.10, 1 hit 1.10.580.10, 1 hit |
InterProi | View protein in InterPro IPR016142, Citrate_synth-like_lrg_a-sub IPR016143, Citrate_synth-like_sm_a-sub IPR002020, Citrate_synthase IPR019810, Citrate_synthase_AS IPR024176, Citrate_synthase_bac-typ IPR036969, Citrate_synthase_sf |
PANTHERi | PTHR11739, PTHR11739, 1 hit |
Pfami | View protein in Pfam PF00285, Citrate_synt, 1 hit |
PIRSFi | PIRSF001369, Citrate_synth, 1 hit |
PRINTSi | PR00143, CITRTSNTHASE |
SUPFAMi | SSF48256, SSF48256, 1 hit |
PROSITEi | View protein in PROSITE PS00480, CITRATE_SYNTHASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MMGD_BACSU | |
Accessioni | P45858Primary (citable) accession number: P45858 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families