UniProtKB - P45759 (GSPE_ECOLI)
Protein
Putative type II secretion system protein E
Gene
gspE
Organism
Escherichia coli (strain K12)
Status
Functioni
ATPase component of the type II secretion system required for the energy-dependent secretion of extracellular factors such as proteases and toxins from the periplasm. Acts as a molecular motor to provide the energy that is required for assembly of the pseudopilus and the extrusion of substrates generated in the cytoplasm.By similarity
Miscellaneous
Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.
Cofactori
Zn2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 382 | ZincBy similarity | 1 | |
Metal bindingi | 385 | ZincBy similarity | 1 | |
Metal bindingi | 415 | ZincBy similarity | 1 | |
Metal bindingi | 418 | ZincBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 249 – 256 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- protein secretion by the type II secretion system Source: GO_Central
Keywordsi
Biological process | Protein transport, Transport |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:G7704-MONOMER MetaCyc:G7704-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Putative type II secretion system protein EShort name: T2SS protein E Alternative name(s): Putative general secretion pathway protein E Type II traffic warden ATPase |
Gene namesi | Name:gspE Synonyms:yheG Ordered Locus Names:b3326, JW3288 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane By similarity Note: Membrane association is not an intrinsic property but requires the GspL gene product.By similarity
GO - Cellular componenti
- plasma membrane Source: GO_Central
- type II protein secretion system complex Source: GO_Central
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207284 | 1 – 493 | Putative type II secretion system protein EAdd BLAST | 493 |
Proteomic databases
PaxDbi | P45759 |
PRIDEi | P45759 |
Expressioni
Inductioni
Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication
Interactioni
Subunit structurei
Forms homooligomers; most probably hexamers (By similarity).
Interacts with GspL (By similarity).
By similarityBinary interactionsi
P45759
With | #Exp. | IntAct |
---|---|---|
rplB [P60422] | 2 | EBI-1132437,EBI-543515 |
Protein-protein interaction databases
BioGRIDi | 4261298, 330 interactors 852135, 1 interactor |
IntActi | P45759, 1 interactor |
STRINGi | 511145.b3326 |
Family & Domainsi
Sequence similaritiesi
Belongs to the GSP E family.Curated
Phylogenomic databases
eggNOGi | COG2804, Bacteria |
HOGENOMi | CLU_013446_10_3_6 |
InParanoidi | P45759 |
PhylomeDBi | P45759 |
Family and domain databases
Gene3Di | 3.30.300.160, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR027417, P-loop_NTPase IPR001482, T2SS/T4SS IPR013369, T2SS_GspE IPR042181, T2SS_GspE_N_sf |
Pfami | View protein in Pfam PF00437, T2SSE, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR02533, type_II_gspE, 1 hit |
PROSITEi | View protein in PROSITE PS00662, T2SP_E, 1 hit |
i Sequence
Sequence statusi: Complete.
P45759-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRIHSPYPAS WALAQRIGYL YSEGEIIYLA DTPFERLLDI QRQVGQCQTM
60 70 80 90 100
TSLSQADFEA RLEAVFHQNT GESQQIAQDI DQSVDLLSLS EEMPANEDLL
110 120 130 140 150
NEDSAAPVIR LINAILSEAI KETASDIHIE TYEKTMSIRF RIDGVLRTIL
160 170 180 190 200
QPNKKLAALL ISRIKVMARL DIAEKRIPQD GRISLRIGRR NIDVRVSTLP
210 220 230 240 250
SIYGERAVLR LLDKNSLQLS LNNLGMTAAD KQDLENLIQL PHGIILVTGP
260 270 280 290 300
TGSGKSTTLY AILSALNTPG RNILTVEDPV EYELEGIGQT QVNTRVDMSF
310 320 330 340 350
ARGLRAILRQ DPDVVMVGEI RDTETAQIAV QASLTGHLVL STLHTNSASG
360 370 380 390 400
AVTRLRDMGV ESFLLSSSLA GIIAQRLVRR LCPQCRQFTP VSPQQAQMFK
410 420 430 440 450
YHQLAVTTIG TPVGCPHCHQ SGYQGRMAIH EMMVVTPELR AAIHENVDEQ
460 470 480 490
ALERLVRQQH KALIKNGLQK VISGDTSWDE VMRVASATLE SEA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58123.1 U00096 Genomic DNA Translation: AAC76351.1 AP009048 Genomic DNA Translation: BAE77965.1 |
PIRi | A65126 |
RefSeqi | NP_417785.1, NC_000913.3 WP_001219894.1, NZ_SSZK01000040.1 |
Genome annotation databases
EnsemblBacteriai | AAC76351; AAC76351; b3326 BAE77965; BAE77965; BAE77965 |
GeneIDi | 947823 |
KEGGi | ecj:JW3288 eco:b3326 |
PATRICi | fig|1411691.4.peg.3405 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58123.1 U00096 Genomic DNA Translation: AAC76351.1 AP009048 Genomic DNA Translation: BAE77965.1 |
PIRi | A65126 |
RefSeqi | NP_417785.1, NC_000913.3 WP_001219894.1, NZ_SSZK01000040.1 |
3D structure databases
SMRi | P45759 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261298, 330 interactors 852135, 1 interactor |
IntActi | P45759, 1 interactor |
STRINGi | 511145.b3326 |
Proteomic databases
PaxDbi | P45759 |
PRIDEi | P45759 |
Genome annotation databases
EnsemblBacteriai | AAC76351; AAC76351; b3326 BAE77965; BAE77965; BAE77965 |
GeneIDi | 947823 |
KEGGi | ecj:JW3288 eco:b3326 |
PATRICi | fig|1411691.4.peg.3405 |
Organism-specific databases
EchoBASEi | EB2728 |
Phylogenomic databases
eggNOGi | COG2804, Bacteria |
HOGENOMi | CLU_013446_10_3_6 |
InParanoidi | P45759 |
PhylomeDBi | P45759 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7704-MONOMER MetaCyc:G7704-MONOMER |
Miscellaneous databases
PROi | PR:P45759 |
Family and domain databases
Gene3Di | 3.30.300.160, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR027417, P-loop_NTPase IPR001482, T2SS/T4SS IPR013369, T2SS_GspE IPR042181, T2SS_GspE_N_sf |
Pfami | View protein in Pfam PF00437, T2SSE, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR02533, type_II_gspE, 1 hit |
PROSITEi | View protein in PROSITE PS00662, T2SP_E, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GSPE_ECOLI | |
Accessioni | P45759Primary (citable) accession number: P45759 Secondary accession number(s): Q2M6Z1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families