UniProtKB - P45758 (GSPD_ECOLI)
Protein
Putative secretin GspD
Gene
gspD
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of folded proteins across the outer membrane. This subunit would form the outer membrane channel.By similarity
Miscellaneous
Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 461 | May serve as a pivot that allows opening of the central gate for substrate egressBy similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: IntAct
GO - Biological processi
- protein secretion by the type II secretion system Source: InterPro
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:G7703-MONOMER ECOL316407:JW5707-MONOMER MetaCyc:G7703-MONOMER |
Protein family/group databases
TCDBi | 1.B.22.1.3 the outer bacterial membrane secretin (secretin) family |
Names & Taxonomyi
Protein namesi | Recommended name: Putative secretin GspD1 PublicationAlternative name(s): Putative general secretion pathway protein D Putative type II secretion system protein D Short name: T2SS protein D |
Gene namesi | Name:gspD Synonyms:yheF Ordered Locus Names:b3325, JW5707 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell outer membrane 1 Publication Note: Most of the protein is in the periplasm which it traverses to contact proteins of the cell inner membrane.1 Publication
GO - Cellular componenti
- cell outer membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
- type II protein secretion system complex Source: InterPro
Keywords - Cellular componenti
Cell outer membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000013099 | 24 – 650 | Putative secretin GspDAdd BLAST | 627 |
Proteomic databases
jPOSTi | P45758 |
PaxDbi | P45758 |
PRIDEi | P45758 |
Expressioni
Inductioni
Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication
Interactioni
Subunit structurei
Forms a cylindrical channel with 15 subunits; approximately 25% of the particles have 16-subunit channels. Closed pentadeacameric channels are 180 Angstroms long and 145 Angstroms in diameter. Each subunit turns in a clock-wise manner around the channel.
1 PublicationBinary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
itself | 3 | EBI-21225187,EBI-21225187 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGridi | 4261297, 269 interactors |
STRINGi | 511145.b3325 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P45758 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 24 – 122 | N01 PublicationAdd BLAST | 99 | |
Regioni | 124 – 188 | N11 PublicationAdd BLAST | 65 | |
Regioni | 189 – 263 | N21 PublicationAdd BLAST | 75 | |
Regioni | 266 – 342 | N31 PublicationAdd BLAST | 77 | |
Regioni | 345 – 596 | Secretin1 PublicationAdd BLAST | 252 | |
Regioni | 598 – 650 | S domain1 PublicationAdd BLAST | 53 |
Domaini
The N0, N1, N2 and N3 domains are periplasmic, while the secretin and S domains form a channel that is partially inserted in the outer membrane. The N1, N2 and N3 domains each form a periplasmic ring; the N0 domain was present but not resolved by electron microscopy. The secretin domain forms a double beta-barrel structure; the outer barrel has a diameter of about 110 Angstroms while the inner barrel forms the central gate with a small pore in the closed state.1 Publication
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane beta strandPhylogenomic databases
eggNOGi | ENOG4105GVR Bacteria COG1450 LUCA |
HOGENOMi | HOG000253751 |
InParanoidi | P45758 |
KOi | K02453 |
PhylomeDBi | P45758 |
Family and domain databases
Gene3Di | 3.30.1370.120, 3 hits |
InterProi | View protein in InterPro IPR001775 GspD/PilQ IPR005644 NolW-like IPR038591 NolW-like_sf IPR004846 T2SS/T3SS IPR013356 T2SS_GspD IPR004845 T2SS_GspD_CS |
Pfami | View protein in Pfam PF00263 Secretin, 1 hit PF03958 Secretin_N, 3 hits |
PRINTSi | PR00811 BCTERIALGSPD |
TIGRFAMsi | TIGR02517 type_II_gspD, 1 hit |
PROSITEi | View protein in PROSITE PS00875 T2SP_D, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P45758-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKGLNKITCC LLAALLMPCA GHAENEQYGA NFNNADIRQF VEIVGQHLGK
60 70 80 90 100
TILIDPSVQG TISVRSNDTF SQQEYYQFFL SILDLYGYSV ITLDNGFLKV
110 120 130 140 150
VRSANVKTSP GMIADSSRPG VGDELVTRIV PLENVPARDL APLLRQMMDA
160 170 180 190 200
GSVGNVVHYE PSNVLILTGR ASTINKLIEV IKRVDVIGTE KQQIIHLEYA
210 220 230 240 250
SAEDLAEILN QLISESHGKS QMPALLSAKI VADKRTNSLI ISGPEKARQR
260 270 280 290 300
ITSLLKSLDV EESEEGNTRV YYLKYAKATN LVEVLTGVSE KLKDEKGNAR
310 320 330 340 350
KPSSSGAMDN VAITADEQTN SLVITADQSV QEKLATVIAR LDIRRAQVLV
360 370 380 390 400
EAIIVEVQDG NGLNLGVQWA NKNVGAQQFT NTGLPIFNAA QGVADYKKNG
410 420 430 440 450
GITSANPAWD MFSAYNGMAA GFFNGDWGVL LTALASNNKN DILATPSIVT
460 470 480 490 500
LDNKLASFNV GQDVPVLSGS QTTSGDNVFN TVERKTVGTK LKVTPQVNEG
510 520 530 540 550
DAVLLEIEQE VSSVDSSSNS TLGPTFNTRT IQNAVLVKTG ETVVLGGLLD
560 570 580 590 600
DFSKEQVSKV PLLGDIPLVG QLFRYTSTER AKRNLMVFIR PTIIRDDDVY
610 620 630 640 650
RSLSKEKYTR YRQEQQQRID GKSKALVGSE DLPVLDENTF NSHAPAPSSR
Sequence cautioni
The sequence AAA58122 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58122.1 Different initiation. U00096 Genomic DNA Translation: AAC76350.2 AP009048 Genomic DNA Translation: BAE77966.1 |
PIRi | H65125 |
RefSeqi | NP_417784.4, NC_000913.3 WP_001326512.1, NZ_SSZK01000040.1 |
Genome annotation databases
EnsemblBacteriai | AAC76350; AAC76350; b3325 BAE77966; BAE77966; BAE77966 |
GeneIDi | 947822 |
KEGGi | ecj:JW5707 eco:b3325 |
PATRICi | fig|1411691.4.peg.3406 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58122.1 Different initiation. U00096 Genomic DNA Translation: AAC76350.2 AP009048 Genomic DNA Translation: BAE77966.1 |
PIRi | H65125 |
RefSeqi | NP_417784.4, NC_000913.3 WP_001326512.1, NZ_SSZK01000040.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5WQ7 | electron microscopy | 3.04 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O | 24-650 | [»] | |
SMRi | P45758 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGridi | 4261297, 269 interactors |
STRINGi | 511145.b3325 |
Protein family/group databases
TCDBi | 1.B.22.1.3 the outer bacterial membrane secretin (secretin) family |
Proteomic databases
jPOSTi | P45758 |
PaxDbi | P45758 |
PRIDEi | P45758 |
Genome annotation databases
EnsemblBacteriai | AAC76350; AAC76350; b3325 BAE77966; BAE77966; BAE77966 |
GeneIDi | 947822 |
KEGGi | ecj:JW5707 eco:b3325 |
PATRICi | fig|1411691.4.peg.3406 |
Organism-specific databases
EchoBASEi | EB2727 |
Phylogenomic databases
eggNOGi | ENOG4105GVR Bacteria COG1450 LUCA |
HOGENOMi | HOG000253751 |
InParanoidi | P45758 |
KOi | K02453 |
PhylomeDBi | P45758 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7703-MONOMER ECOL316407:JW5707-MONOMER MetaCyc:G7703-MONOMER |
Miscellaneous databases
PROi | PR:P45758 |
Family and domain databases
Gene3Di | 3.30.1370.120, 3 hits |
InterProi | View protein in InterPro IPR001775 GspD/PilQ IPR005644 NolW-like IPR038591 NolW-like_sf IPR004846 T2SS/T3SS IPR013356 T2SS_GspD IPR004845 T2SS_GspD_CS |
Pfami | View protein in Pfam PF00263 Secretin, 1 hit PF03958 Secretin_N, 3 hits |
PRINTSi | PR00811 BCTERIALGSPD |
TIGRFAMsi | TIGR02517 type_II_gspD, 1 hit |
PROSITEi | View protein in PROSITE PS00875 T2SP_D, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GSPD_ECOLI | |
Accessioni | P45758Primary (citable) accession number: P45758 Secondary accession number(s): Q2M6Z0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | July 11, 2001 | |
Last modified: | November 13, 2019 | |
This is version 138 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene - PDB cross-references
Index of Protein Data Bank (PDB) cross-references