UniProtKB - P45748 (TSAC_ECOLI)
Threonylcarbamoyl-AMP synthase
tsaC
Functioni
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Catalyzes the conversion of L-threonine, HCO3-/CO2 and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. Is also able to catalyze the reverse reaction in vitro, i.e. the formation of ATP from TC-AMP and PPi. Shows higher affinity for the full-length tRNA(Thr) lacking only the t6A37 modification than for its fully modified counterpart. Could also be required for the maturation of 16S rRNA. Binds to double-stranded RNA but does not interact tightly with either of the ribosomal subunits, or the 70S particles.
UniRule annotation5 PublicationsMiscellaneous
Caution
Catalytic activityi
- ATP + hydrogencarbonate + L-threonine = diphosphate + H2O + L-threonylcarbamoyladenylateUniRule annotation1 PublicationEC:2.7.7.87UniRule annotation1 Publication
Kineticsi
- KM=93 µM for ATP1 Publication
- KM=0.68 µM for L-threonylcarbamoyl-AMP1 Publication
GO - Molecular functioni
- ATP binding Source: EcoCyc
- double-stranded RNA binding Source: EcoCyc
- L-threonylcarbamoyladenylate synthase Source: EcoCyc
- nucleotidyltransferase activity Source: UniProtKB
- tRNA binding Source: EcoCyc
GO - Biological processi
- regulation of translational fidelity Source: GO_Central
- rRNA processing Source: UniProtKB-KW
- tRNA threonylcarbamoyladenosine modification Source: EcoCyc
Keywordsi
Molecular function | Nucleotidyltransferase, RNA-binding, Transferase, tRNA-binding |
Biological process | Ribosome biogenesis, rRNA processing, tRNA processing |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G7698-MONOMER |
BRENDAi | 2.7.7.87, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Threonylcarbamoyl-AMP synthaseUniRule annotation (EC:2.7.7.87UniRule annotation)Short name: TC-AMP synthaseUniRule annotation Alternative name(s): L-threonylcarbamoyladenylate synthaseUniRule annotation Ribosome maturation factor TsaC t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaCUniRule annotation tRNA threonylcarbamoyladenosine biosynthesis protein TsaCUniRule annotation |
Gene namesi | Name:tsaCUniRule annotation Synonyms:rimN, yrdC Ordered Locus Names:b3282, JW3243 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 50 | K → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 52 | R → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 56 | K → A: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 110 | R → A: No change in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000202018 | 1 – 190 | Threonylcarbamoyl-AMP synthaseAdd BLAST | 190 |
Proteomic databases
jPOSTi | P45748 |
PaxDbi | P45748 |
PRIDEi | P45748 |
Interactioni
Subunit structurei
Interacts with TsaB and TsaD.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262454, 224 interactors |
DIPi | DIP-12916N |
IntActi | P45748, 12 interactors |
STRINGi | 511145.b3282 |
Structurei
Secondary structure
3D structure databases
SMRi | P45748 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P45748 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 190 | YrdC-likeUniRule annotationAdd BLAST | 184 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0009, Bacteria |
HOGENOMi | CLU_031397_6_0_6 |
InParanoidi | P45748 |
PhylomeDBi | P45748 |
Family and domain databases
HAMAPi | MF_01852, TsaC, 1 hit |
InterProi | View protein in InterPro IPR017945, DHBP_synth_RibB-like_a/b_dom IPR023535, TC-AMP_synthase IPR006070, YrdC-like_dom |
PANTHERi | PTHR17490:SF8, PTHR17490:SF8, 1 hit |
Pfami | View protein in Pfam PF01300, Sua5_yciO_yrdC, 1 hit |
SUPFAMi | SSF55821, SSF55821, 1 hit |
PROSITEi | View protein in PROSITE PS51163, YRDC, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MNNNLQRDAI AAAIDVLNEE RVIAYPTEAV FGVGCDPDSE TAVMRLLELK
60 70 80 90 100
QRPVDKGLIL IAANYEQLKP YIDDTMLTDV QRETIFSRWP GPVTFVFPAP
110 120 130 140 150
ATTPRWLTGR FDSLAVRVTD HPLVVALCQA YGKPLVSTSA NLSGLPPCRT
160 170 180 190
VDEVRAQFGA AFPVVPGETG GRLNPSEIRD ALTGELFRQG
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58079.1 Frameshift. U00096 Genomic DNA Translation: AAC76307.1 AP009048 Genomic DNA Translation: BAE78009.1 |
PIRi | E65120 |
RefSeqi | NP_417741.1, NC_000913.3 WP_001301412.1, NZ_SSZK01000040.1 |
Genome annotation databases
EnsemblBacteriai | AAC76307; AAC76307; b3282 BAE78009; BAE78009; BAE78009 |
GeneIDi | 947783 |
KEGGi | ecj:JW3243 eco:b3282 |
PATRICi | fig|1411691.4.peg.3449 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58079.1 Frameshift. U00096 Genomic DNA Translation: AAC76307.1 AP009048 Genomic DNA Translation: BAE78009.1 |
PIRi | E65120 |
RefSeqi | NP_417741.1, NC_000913.3 WP_001301412.1, NZ_SSZK01000040.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HRU | X-ray | 2.00 | A/B | 1-188 | [»] | |
2MX1 | NMR | - | A | 2-190 | [»] | |
SMRi | P45748 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262454, 224 interactors |
DIPi | DIP-12916N |
IntActi | P45748, 12 interactors |
STRINGi | 511145.b3282 |
Proteomic databases
jPOSTi | P45748 |
PaxDbi | P45748 |
PRIDEi | P45748 |
Genome annotation databases
EnsemblBacteriai | AAC76307; AAC76307; b3282 BAE78009; BAE78009; BAE78009 |
GeneIDi | 947783 |
KEGGi | ecj:JW3243 eco:b3282 |
PATRICi | fig|1411691.4.peg.3449 |
Organism-specific databases
EchoBASEi | EB2689 |
Phylogenomic databases
eggNOGi | COG0009, Bacteria |
HOGENOMi | CLU_031397_6_0_6 |
InParanoidi | P45748 |
PhylomeDBi | P45748 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7698-MONOMER |
BRENDAi | 2.7.7.87, 2026 |
Miscellaneous databases
EvolutionaryTracei | P45748 |
PROi | PR:P45748 |
Family and domain databases
HAMAPi | MF_01852, TsaC, 1 hit |
InterProi | View protein in InterPro IPR017945, DHBP_synth_RibB-like_a/b_dom IPR023535, TC-AMP_synthase IPR006070, YrdC-like_dom |
PANTHERi | PTHR17490:SF8, PTHR17490:SF8, 1 hit |
Pfami | View protein in Pfam PF01300, Sua5_yciO_yrdC, 1 hit |
SUPFAMi | SSF55821, SSF55821, 1 hit |
PROSITEi | View protein in PROSITE PS51163, YRDC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TSAC_ECOLI | |
Accessioni | P45748Primary (citable) accession number: P45748 Secondary accession number(s): Q2M6U7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 23, 2022 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families