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UniProtKB - P45745 (DHBF_BACSU)
Protein
Dimodular nonribosomal peptide synthase
Gene
dhbF
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Specifically adenylates L-threonine and, to a lesser extent, glycine and covalently loads both amino acids onto their corresponding peptidyl carrier domains.
1 PublicationCaution
The phosphoserine observed at Ser-996 in PubMed:17218307 undoubtedly results from the secondary neutral loss of pantetheine from the phosphodiester linked cofactor.Curated
Catalytic activityi
- ATP + holo-[peptidyl-carrier protein] + L-threonine = AMP + diphosphate + L-threonyl-[peptidyl-carrier protein]1 PublicationEC:6.2.1.701 PublicationThis reaction proceeds in the forward1 Publication direction.
- ATP + glycine + holo-[peptidyl-carrier protein] = AMP + diphosphate + glycyl-[peptidyl-carrier protein]1 PublicationEC:6.2.1.661 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
pantetheine 4'-phosphateCurated
: bacillibactin biosynthesis Pathwayi
This protein is involved in the pathway bacillibactin biosynthesis, which is part of Siderophore biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway bacillibactin biosynthesis and in Siderophore biosynthesis.
GO - Molecular functioni
- ligase activity Source: UniProtKB-KW
- phosphopantetheine binding Source: InterPro
GO - Biological processi
- biosynthetic process Source: InterPro
Keywordsi
Molecular function | Ligase |
Enzyme and pathway databases
BioCyci | BSUB:BSU31960-MONOMER |
UniPathwayi | UPA00013 |
Protein family/group databases
ESTHERi | bacsu-YUKL, Thioesterase |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dhbF Ordered Locus Names:BSU31960 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193081 | 1 – 2378 | Dimodular nonribosomal peptide synthaseAdd BLAST | 2378 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 996 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 2071 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Phosphopantetheine, PhosphoproteinProteomic databases
jPOSTi | P45745 |
PaxDbi | P45745 |
PRIDEi | P45745 |
Interactioni
Protein-protein interaction databases
IntActi | P45745, 3 interactors |
MINTi | P45745 |
STRINGi | 224308.BSU31960 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 961 – 1036 | Carrier 1PROSITE-ProRule annotationAdd BLAST | 76 | |
Domaini | 2036 – 2111 | Carrier 2PROSITE-ProRule annotationAdd BLAST | 76 |
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG1020, Bacteria |
InParanoidi | P45745 |
OMAi | FQIAPEM |
PhylomeDBi | P45745 |
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit 3.30.300.30, 2 hits 3.30.559.10, 2 hits 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR029058, AB_hydrolase IPR036736, ACP-like_sf IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR023213, CAT-like_dom_sf IPR001242, Condensatn IPR020806, PKS_PP-bd IPR020802, PKS_thioesterase IPR009081, PP-bd_ACP IPR001031, Thioesterase |
Pfami | View protein in Pfam PF00501, AMP-binding, 2 hits PF13193, AMP-binding_C, 2 hits PF00668, Condensation, 2 hits PF00550, PP-binding, 2 hits PF00975, Thioesterase, 1 hit |
SMARTi | View protein in SMART SM00823, PKS_PP, 2 hits SM00824, PKS_TE, 1 hit |
SUPFAMi | SSF47336, SSF47336, 2 hits SSF53474, SSF53474, 1 hit |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 2 hits |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 2 hits PS50075, CARRIER, 2 hits |
i Sequence
Sequence statusi: Complete.
P45745-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPDTKDLQYS LTGAQTGIWF AQQLDPDNPI YNTAEYIEIN GPVNIALFEE
60 70 80 90 100
ALRHVIKEAE SLHVRFGENM DGPWQMINPS PDVQLHVIDV SSEPDPEKTA
110 120 130 140 150
LNWMKADLAK PVDLGYAPLF NEALFIAGPD RFFWYQRIHH IAIDGFGFSL
160 170 180 190 200
IAQRVASTYT ALIKGQTAKS RSFGSLQAIL EEDTDYRGSE QYEKDRQFWL
210 220 230 240 250
DRFADAPEVV SLADRAPRTS NSFLRHTAYL PPSDVNALKE AARYFSGSWH
260 270 280 290 300
EVMIAVSAVY VHRMTGSEDV VLGLPMMGRI GSASLNVPAM VMNLLPLRLT
310 320 330 340 350
VSSSMSFSEL IQQISREIRS IRRHHKYRHE ELRRDLKLIG ENHRLFGPQI
360 370 380 390 400
NLMPFDYGLD FAGVRGTTHN LSAGPVDDLS INVYDRTDGS GLRIDVDANP
410 420 430 440 450
EVYSESDIKL HQQRILQLLQ TASAGEDMLI GQMELLLPEE KEKVISKWNE
460 470 480 490 500
TAKSEKLVSL QDMFEKQAVL TPERIALMCD DIQVNYRKLN EEANRLARLL
510 520 530 540 550
IEKGIGPEQF VALALPRSPE MVASMLGVLK TGAAYLPLDP EFPADRISYM
560 570 580 590 600
LEDAKPSCII TTEEIAASLP DDLAVPELVL DQAVTQEIIK RYSPENQDVS
610 620 630 640 650
VSLDHPAYII YTSGSTGRPK GVVVTQKSLS NFLLSMQEAF SLGEEDRLLA
660 670 680 690 700
VTTVAFDISA LELYLPLISG AQIVIAKKET IREPQALAQM IENFDINIMQ
710 720 730 740 750
ATPTLWHALV TSEPEKLRGL RVLVGGEALP SGLLQELQDL HCSVTNLYGP
760 770 780 790 800
TETTIWSAAA FLEEGLKGVP PIGKPIWNTQ VYVLDNGLQP VPPGVVGELY
810 820 830 840 850
IAGTGLARGY FHRPDLTAER FVADPYGPPG TRMYRTGDQA RWRADGSLDY
860 870 880 890 900
IGRADHQIKI RGFRIELGEI DAVLANHPHI EQAAVVVRED QPGDKRLAAY
910 920 930 940 950
VVADAAIDTA ELRRYMGASL PDYMVPSAFV EMDELPLTPN GKLDRKALPA
960 970 980 990 1000
PDFSTSVSDR APRTPQEEIL CDLFAEVLGL ARVGIDDSFF ELGGHSLLAA
1010 1020 1030 1040 1050
RLMSRIREVM GAELGIAKLF DEPTVAGLAA HLDLAQSACP ALQRAERPEK
1060 1070 1080 1090 1100
IPLSFAQRRL WFLHCLEGPS PTYNIPVAVR LSGELDQGLL KAALYDLVCR
1110 1120 1130 1140 1150
HESLRTIFPE SQGTSYQHIL DADRACPELH VTEIAEKELS DRLAEAVRYS
1160 1170 1180 1190 1200
FDLAAEPAFR AELFVIGPDE YVLLLLVHHI VGDGWSLTPL TRDLGTAYAA
1210 1220 1230 1240 1250
RCHGRSPEWA PLAVQYADYA LWQQELLGNE DDPNSLIAGQ LAFWKETLKN
1260 1270 1280 1290 1300
LPDQLELPTD YSRPAEPSHD GDTIHFRIEP EFHKRLQELA RANRVSLFMV
1310 1320 1330 1340 1350
LQSGLAALLT RLGAGTDIPI GSPIAGRNDD ALGDLVGLFI NTLVLRTDTS
1360 1370 1380 1390 1400
GDPSFRELLD RVREVNLAAY DNQDLPFERL VEVLNPARSR ATHPLFQIML
1410 1420 1430 1440 1450
AFQNTPDAEL HLPDMESSLR INSVGSAKFD LTLEISEDRL ADGTPNGMEG
1460 1470 1480 1490 1500
LLEYSTDLFK RETAQALADR LMRLLEAAES DPDEQIGNLD ILAPEEHSSM
1510 1520 1530 1540 1550
VTDWQSVSEK IPHACLPEQF EKQAALRPDA IAVVYENQEL SYAELNERAN
1560 1570 1580 1590 1600
RLARMMISEG VGPEQFVALA LPRSLEMAVG LLAVLKAGAA YLPLDPDYPA
1610 1620 1630 1640 1650
DRIAFMLKDA QPAFIMTNTK AANHIPPVEN VPKIVLDDPE LAEKLNTYPA
1660 1670 1680 1690 1700
GNPKNKDRTQ PLSPLNTAYV IYTSGSTGVP KGVMIPHQNV TRLFAATEHW
1710 1720 1730 1740 1750
FRFSSGDIWT MFHSYAFDFS VWEIWGPLLH GGRLVIVPHH VSRSPEAFLR
1760 1770 1780 1790 1800
LLVKEGVTVL NQTPSAFYQF MQAEREQPDL GQALSLRYVI FGGEALELSR
1810 1820 1830 1840 1850
LEDWYNRHPE NRPQLINMYG ITETTVHVSY IELDRSMAAL RANSLIGCGI
1860 1870 1880 1890 1900
PDLGVYVLDE RLQPVPPGVA GELYVSGAGL ARGYLGRPGL TSERFIADPF
1910 1920 1930 1940 1950
GPPGTRMYRT GDVARLRADG SLDYVGRADH QVKIRGFRIE LGEIEAALVQ
1960 1970 1980 1990 2000
HPQLEDAAVI VREDQPGDKR LAAYVIPSEE TFDTAELRRY AAERLPDYMV
2010 2020 2030 2040 2050
PAAFVTMKEL PLTPNGKLDR KALPAPDFAA AVTGRGPRTP QEEILCDLFM
2060 2070 2080 2090 2100
EVLHLPRVGI DDRFFDLGGH SLLAVQLMSR IREALGVELS IGNLFEAPTV
2110 2120 2130 2140 2150
AGLAERLEMG SSQSALDVLL PLRTSGDKPP LFCVHPAGGL SWCYAGLMTN
2160 2170 2180 2190 2200
IGTDYPIYGL QARGIGQREE LPKTLDDMAA DYIKQIRTVQ PKGPYHLLGW
2210 2220 2230 2240 2250
SLGGNVVQAM ATQLQNQGEE VSLLVMLDAY PNHFLPIKEA PDDEEALIAL
2260 2270 2280 2290 2300
LALGGYDPDS LGEKPLDFEA AIEILRRDGS ALASLDETVI LNLKNTYVNS
2310 2320 2330 2340 2350
VGILGSYKPK TFRGNVLFFR STIIPEWFDP IEPDSWKPYI NGQIEQIDID
2360 2370
CRHKDLCQPE PLAQIGKVLA VKLEELNK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2141 | S → G in AAD56240 (PubMed:11112781).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF184977 Genomic DNA Translation: AAD56240.1 AL009126 Genomic DNA Translation: CAB15186.3 U26444 Genomic DNA Translation: AAC44634.1 |
PIRi | E69615 |
RefSeqi | NP_391076.3, NC_000964.3 WP_009968094.1, NZ_JNCM01000033.1 |
Genome annotation databases
EnsemblBacteriai | CAB15186; CAB15186; BSU_31960 |
GeneIDi | 936569 |
KEGGi | bsu:BSU31960 |
PATRICi | fig|224308.179.peg.3462 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF184977 Genomic DNA Translation: AAD56240.1 AL009126 Genomic DNA Translation: CAB15186.3 U26444 Genomic DNA Translation: AAC44634.1 |
PIRi | E69615 |
RefSeqi | NP_391076.3, NC_000964.3 WP_009968094.1, NZ_JNCM01000033.1 |
3D structure databases
SMRi | P45745 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P45745, 3 interactors |
MINTi | P45745 |
STRINGi | 224308.BSU31960 |
Protein family/group databases
ESTHERi | bacsu-YUKL, Thioesterase |
Proteomic databases
jPOSTi | P45745 |
PaxDbi | P45745 |
PRIDEi | P45745 |
Genome annotation databases
EnsemblBacteriai | CAB15186; CAB15186; BSU_31960 |
GeneIDi | 936569 |
KEGGi | bsu:BSU31960 |
PATRICi | fig|224308.179.peg.3462 |
Phylogenomic databases
eggNOGi | COG1020, Bacteria |
InParanoidi | P45745 |
OMAi | FQIAPEM |
PhylomeDBi | P45745 |
Enzyme and pathway databases
UniPathwayi | UPA00013 |
BioCyci | BSUB:BSU31960-MONOMER |
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit 3.30.300.30, 2 hits 3.30.559.10, 2 hits 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR029058, AB_hydrolase IPR036736, ACP-like_sf IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR023213, CAT-like_dom_sf IPR001242, Condensatn IPR020806, PKS_PP-bd IPR020802, PKS_thioesterase IPR009081, PP-bd_ACP IPR001031, Thioesterase |
Pfami | View protein in Pfam PF00501, AMP-binding, 2 hits PF13193, AMP-binding_C, 2 hits PF00668, Condensation, 2 hits PF00550, PP-binding, 2 hits PF00975, Thioesterase, 1 hit |
SMARTi | View protein in SMART SM00823, PKS_PP, 2 hits SM00824, PKS_TE, 1 hit |
SUPFAMi | SSF47336, SSF47336, 2 hits SSF53474, SSF53474, 1 hit |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 2 hits |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 2 hits PS50075, CARRIER, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | DHBF_BACSU | |
Accessioni | P45745Primary (citable) accession number: P45745 Secondary accession number(s): Q9R9I2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | July 28, 2009 | |
Last modified: | February 23, 2022 | |
This is version 152 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families