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UniProtKB - P45701 (MA1A1_RABIT)
Protein
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
Gene
MAN1A1
Organism
Oryctolagus cuniculus (Rabbit)
Status
Functioni
Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.
Catalytic activityi
- 4 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
- 3 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
Cofactori
Ca2+By similarity
Activity regulationi
Inhibited by both 1-deoxymannojirimycin and kifunensine.By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 338 | Proton donorBy similarity | 1 | |
Metal bindingi | 449 | CalciumBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- protein glycosylation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
Names & Taxonomyi
Protein namesi | Recommended name: Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC:3.2.1.113By similarity)Alternative name(s): Man(9)-alpha-mannosidase Mannosidase alpha class 1A member 1 Processing alpha-1,2-mannosidase IA Short name: Alpha-1,2-mannosidase IA |
Gene namesi | Name:MAN1A1 |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Golgi apparatus
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | ‹1 – 469 | LumenalSequence analysisAdd BLAST | ›469 |
Keywords - Cellular componenti
Golgi apparatusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210311 | ‹1 – 469 | Mannosyl-oligosaccharide 1,2-alpha-mannosidase IAAdd BLAST | ›469 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 292 ↔ 324 | By similarity | ||
Glycosylationi | 329 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinFamily & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 47 family.Curated
Keywords - Domaini
Signal-anchor, TransmembranePhylogenomic databases
eggNOGi | KOG2204, Eukaryota |
InParanoidi | P45701 |
OMAi | QKVMNIR |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
i Sequence
Sequence statusi: Fragment.
P45701-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
REPADAAVRE KRAKIKEMME HAWNSYKRYA WGLNELKPIT KEGHSSSLFG
60 70 80 90 100
TIKGATIVDA LDTLFIMGME SEFQEAKSWI AENLDFNVNA EISVFEVNIR
110 120 130 140 150
FVGGLLSAYY LSGEEIFRKK AVELGIKLLP AFHTPSGIPW ALLNIKSGIG
160 170 180 190 200
RNWPWASGGS SILAEFGTLH LEFMHLSHLS GNPIFAEKVM NIRKVLNKLE
210 220 230 240 250
KPEGLYPNYL NPSSGQWGQH HVSIGGLGDS FYEYLLKAWL MSEKTDLEAK
260 270 280 290 300
KMYFDAVQAI ETHLIRKSSG GLTYIAEWKG GLLEHKMGHL TCFAGGMFAL
310 320 330 340 350
GADGAPEGRA QHYLELGAEI ARTCHESYNR TFMKLGPEAF RFDGGVEAIA
360 370 380 390 400
TRQNEKYYIL RPEVVETYMY MWRLTHDPKY RKWAWEAVEA LESHCRVNGG
410 420 430 440 450
YSGLRDVYFT HEKYDNVQQS FFLAETLKYL YLIFSDDDLL PLEHWIFNTE
460
AHLLPILPTD QKEVEVKVK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U04301 mRNA Translation: AAA17748.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U04301 mRNA Translation: AAA17748.1 |
3D structure databases
SMRi | P45701 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000005793 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
Phylogenomic databases
eggNOGi | KOG2204, Eukaryota |
InParanoidi | P45701 |
OMAi | QKVMNIR |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MA1A1_RABIT | |
Accessioni | P45701Primary (citable) accession number: P45701 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families