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UniProtKB - P45575 (DSVB_DESVH)
Protein
Sulfite reductase, dissimilatory-type subunit beta
Gene
dsvB
Organism
Desulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / NCIMB 8303 / VKM B-1760 / Hildenborough)
Status
Functioni
Catalyzes the reduction of sulfite to sulfide. This is the terminal oxidation reaction in sulfate respiration, a process catalyzed by the sulfate-reducing bacteria.
2 PublicationsCatalytic activityi
- [DsrC protein]-disulfide + 2 A + 3 H2O + hydrogen sulfide = [DsrC protein]-dithiol + 2 AH2 + H+ + sulfite2 PublicationsEC:1.8.99.52 Publications
- [DsrC protein]-S-sulfanyl-L-cysteine/L-cysteine + 3 A + 3 H2O = [DsrC protein]-disulfide + 3 AH2 + 2 H+ + sulfite2 PublicationsEC:1.8.99.52 Publications
Cofactori
Protein has several cofactor binding sites:- [4Fe-4S] cluster1 PublicationNote: Binds 2 [4Fe-4S] clusters per subunit.1 Publication
- siroheme1 PublicationNote: Binds 1 siroheme per subunit.1 Publication
Kineticsi
kcat is 0.31 (sec-1) for sulfite. kcat is 0.038 (sec-1) for nitrite.1 Publication
- KM=0.06 mM for sulfite1 Publication
- KM=0.028 mM for nitrite1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 151 | Iron-sulfur (4Fe-4S) 1Combined sources1 Publication | 1 | |
Metal bindingi | 188 | Iron-sulfur (4Fe-4S) 1Combined sources1 Publication | 1 | |
Metal bindingi | 189 | Iron-sulfur (4Fe-4S) 1Combined sources1 Publication | 1 | |
Metal bindingi | 193 | Iron (siroheme axial ligand)1 Publication | 1 | |
Metal bindingi | 193 | Iron-sulfur (4Fe-4S) 1Combined sources1 Publication | 1 | |
Metal bindingi | 231 | Iron-sulfur (4Fe-4S) 2Combined sources1 Publication | 1 | |
Metal bindingi | 258 | Iron-sulfur (4Fe-4S) 2Combined sources1 Publication | 1 | |
Metal bindingi | 261 | Iron-sulfur (4Fe-4S) 2Combined sources1 Publication | 1 | |
Metal bindingi | 264 | Iron-sulfur (4Fe-4S) 2Combined sources1 Publication | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- electron transfer activity Source: InterPro
- heme binding Source: InterPro
- hydrogensulfite reductase activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- sulfur compound metabolic process Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | DVUL882:G1GT1-424-MONOMER |
BRENDAi | 1.8.99.5, 1914 |
Names & Taxonomyi
Protein namesi | Recommended name: Sulfite reductase, dissimilatory-type subunit beta (EC:1.8.99.52 Publications)Alternative name(s): Desulfoviridin subunit beta1 Publication Dissimilatory sulfite reductase subunit beta1 Publication Short name: dSiR beta1 Publication Hydrogensulfite reductase subunit beta |
Gene namesi | Name:dsvB Synonyms:dsrB1 Publication Ordered Locus Names:DVU_0403 |
Organismi | Desulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / NCIMB 8303 / VKM B-1760 / Hildenborough) |
Taxonomic identifieri | 882 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000080032 | 2 – 381 | Sulfite reductase, dissimilatory-type subunit betaAdd BLAST | 380 |
Proteomic databases
PaxDbi | P45575 |
Interactioni
Subunit structurei
Heterohexamer of two alpha, two beta and two gamma subunits.
2 PublicationsBinary interactionsi
P45575
With | #Exp. | IntAct |
---|---|---|
dsvA [P45574] | 5 | EBI-9016987,EBI-9016991 |
Protein-protein interaction databases
IntActi | P45575, 4 interactors |
STRINGi | 882.DVU_0403 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P45575 |
SMRi | P45575 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P45575 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 249 – 276 | 4Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 28 |
Phylogenomic databases
eggNOGi | COG2221, Bacteria |
HOGENOMi | CLU_044442_0_0_7 |
OMAi | HTQGWLH |
PhylomeDBi | P45575 |
Family and domain databases
Gene3Di | 3.30.413.10, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR011808, DsrB IPR005117, NiRdtase/SiRdtase_haem-b_fer IPR036136, Nit/Sulf_reduc_fer-like_dom_sf IPR006067, NO2/SO3_Rdtase_4Fe4S_dom IPR045169, NO2/SO3_Rdtase_4Fe4S_prot IPR045854, NO2/SO3_Rdtase_4Fe4S_sf |
PANTHERi | PTHR11493, PTHR11493, 1 hit |
Pfami | View protein in Pfam PF01077, NIR_SIR, 1 hit PF03460, NIR_SIR_ferr, 1 hit |
SUPFAMi | SSF55124, SSF55124, 1 hit SSF56014, SSF56014, 1 hit |
TIGRFAMsi | TIGR02066, dsrB, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P45575-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAFISSGYNP EKPMANRITD IGPRKFDEFF PPVIAKNFGS WLYHEILEPG
60 70 80 90 100
VLMHVAESGD KVYTVRVGAA RLMSITHIRE MCDIADKYCG GHLRFTTRNN
110 120 130 140 150
VEFMVADEAS LKALKEDLAS RKFDGGSLKF PIGGTGAGVS NIVHTQGWVH
160 170 180 190 200
CHTPATDASG PVKAIMDEVF EDFQSMRLPA PVRISLACCI NMCGAVHCSD
210 220 230 240 250
IGVVGIHRKP PMIDHEWTDQ LCEIPLAVAS CPTAAVRPTK LEIGDKKVNT
260 270 280 290 300
IAIKNERCMY CGNCYTMCPA LPISDGEGDG VVIMVGGKVS NRISMPKFSK
310 320 330 340 350
VVVAYIPNEP PRWPSLTKTI KHIIEVYSAN AYKYERLGEW AERIGWERFF
360 370 380
SLTGLEFSHH LIDDFRDPAY YTWRQSTQFK F
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U16723 Genomic DNA Translation: AAA70108.1 AE017285 Genomic DNA Translation: AAS94886.1 |
PIRi | S21238 |
RefSeqi | WP_010937710.1, NZ_CABHLV010000001.1 YP_009627.1, NC_002937.3 |
Genome annotation databases
EnsemblBacteriai | AAS94886; AAS94886; DVU_0403 |
KEGGi | dvu:DVU_0403 |
PATRICi | fig|882.5.peg.380 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U16723 Genomic DNA Translation: AAA70108.1 AE017285 Genomic DNA Translation: AAS94886.1 |
PIRi | S21238 |
RefSeqi | WP_010937710.1, NZ_CABHLV010000001.1 YP_009627.1, NC_002937.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2V4J | X-ray | 2.10 | B/E | 1-381 | [»] | |
AlphaFoldDBi | P45575 | |||||
SMRi | P45575 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P45575, 4 interactors |
STRINGi | 882.DVU_0403 |
Proteomic databases
PaxDbi | P45575 |
Genome annotation databases
EnsemblBacteriai | AAS94886; AAS94886; DVU_0403 |
KEGGi | dvu:DVU_0403 |
PATRICi | fig|882.5.peg.380 |
Phylogenomic databases
eggNOGi | COG2221, Bacteria |
HOGENOMi | CLU_044442_0_0_7 |
OMAi | HTQGWLH |
PhylomeDBi | P45575 |
Enzyme and pathway databases
BioCyci | DVUL882:G1GT1-424-MONOMER |
BRENDAi | 1.8.99.5, 1914 |
Miscellaneous databases
EvolutionaryTracei | P45575 |
Family and domain databases
Gene3Di | 3.30.413.10, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR011808, DsrB IPR005117, NiRdtase/SiRdtase_haem-b_fer IPR036136, Nit/Sulf_reduc_fer-like_dom_sf IPR006067, NO2/SO3_Rdtase_4Fe4S_dom IPR045169, NO2/SO3_Rdtase_4Fe4S_prot IPR045854, NO2/SO3_Rdtase_4Fe4S_sf |
PANTHERi | PTHR11493, PTHR11493, 1 hit |
Pfami | View protein in Pfam PF01077, NIR_SIR, 1 hit PF03460, NIR_SIR_ferr, 1 hit |
SUPFAMi | SSF55124, SSF55124, 1 hit SSF56014, SSF56014, 1 hit |
TIGRFAMsi | TIGR02066, dsrB, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DSVB_DESVH | |
Accessioni | P45575Primary (citable) accession number: P45575 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 140 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references