UniProtKB - P45562 (XAPB_ECOLI)
Protein
Xanthosine permease
Gene
xapB
Organism
Escherichia coli (strain K12)
Status
Functioni
Uptake of xanthosine. Driven by a proton motive force. Can also transport other nucleosides such as inosine, adenosine, cytidine, uridine and thymidine.1 Publication
Activity regulationi
Transport is abolished by 2,4-dinitrophenol.1 Publication
Kineticsi
- KM=136 µM for xanthosine1 Publication
GO - Molecular functioni
- cytidine transmembrane transporter activity Source: GO_Central
- nucleoside transmembrane transporter activity Source: EcoliWiki
- uridine transmembrane transporter activity Source: GO_Central
- xanthosine transmembrane transporter activity Source: EcoliWiki
GO - Biological processi
- nucleobase-containing small molecule metabolic process Source: EcoliWiki
- nucleoside transport Source: EcoliWiki
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:XAPB-MONOMER ECOL316407:JW2397-MONOMER MetaCyc:XAPB-MONOMER |
Protein family/group databases
TCDBi | 2.A.1.10.2 the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Xanthosine permease1 PublicationAlternative name(s): Xanthosine transporterCurated |
Gene namesi | Name:xapB1 Publication Ordered Locus Names:b2406, JW2397 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 3 Publications; Multi-pass membrane protein Sequence analysis
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | Cytoplasmic1 Publication | 9 | |
Transmembranei | 10 – 30 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 31 – 41 | Periplasmic1 PublicationAdd BLAST | 11 | |
Transmembranei | 42 – 62 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 63 – 70 | Cytoplasmic1 Publication | 8 | |
Transmembranei | 71 – 91 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 92 – 112 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 113 – 136 | Cytoplasmic1 PublicationAdd BLAST | 24 | |
Transmembranei | 137 – 157 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 158 – 159 | Periplasmic1 Publication | 2 | |
Transmembranei | 160 – 180 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 181 – 209 | Cytoplasmic1 PublicationAdd BLAST | 29 | |
Transmembranei | 210 – 230 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 231 – 254 | Periplasmic1 PublicationAdd BLAST | 24 | |
Transmembranei | 255 – 275 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 276 – 277 | Cytoplasmic1 Publication | 2 | |
Transmembranei | 278 – 298 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 299 – 306 | Periplasmic1 Publication | 8 | |
Transmembranei | 307 – 327 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 328 – 348 | Cytoplasmic1 PublicationAdd BLAST | 21 | |
Transmembranei | 349 – 369 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 370 – 381 | Periplasmic1 PublicationAdd BLAST | 12 | |
Transmembranei | 382 – 402 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 403 – 418 | Cytoplasmic1 Publication1 PublicationAdd BLAST | 16 |
GO - Cellular componenti
- integral component of membrane Source: EcoliWiki
- integral component of plasma membrane Source: GO_Central
- membrane Source: EcoliWiki
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutant grows very slowly on xanthosine.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000066003 | 1 – 418 | Xanthosine permeaseAdd BLAST | 418 |
Proteomic databases
jPOSTi | P45562 |
PaxDbi | P45562 |
PRIDEi | P45562 |
Interactioni
Protein-protein interaction databases
BioGridi | 4262006, 20 interactors |
STRINGi | 511145.b2406 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 310 – 313 | Poly-LeuSequence analysis | 4 |
Sequence similaritiesi
Belongs to the major facilitator superfamily. Nucleoside:H(+) symporter (NHS) (TC 2.A.1.10) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG4105EAA Bacteria ENOG410ZVFU LUCA |
HOGENOMi | HOG000026502 |
InParanoidi | P45562 |
KOi | K11537 |
PhylomeDBi | P45562 |
Family and domain databases
InterProi | View protein in InterPro IPR020846 MFS_dom IPR036259 MFS_trans_sf IPR004740 Nuc_H_symport |
Pfami | View protein in Pfam PF03825 Nuc_H_symport, 1 hit |
SUPFAMi | SSF103473 SSF103473, 1 hit |
TIGRFAMsi | TIGR00889 2A0110, 1 hit |
PROSITEi | View protein in PROSITE PS50850 MFS, 1 hit |
i Sequence
Sequence statusi: Complete.
P45562-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSIAMRLKVM SFLQYFIWGS WLVTLGSYMI NTLHFTGANV GMVYSSKGIA
60 70 80 90 100
AIIMPGIMGI IADKWLRAER AYMLCHLVCA GVLFYAASVT DPDMMFWVML
110 120 130 140 150
VNAMAFMPTI ALSNSVSYSC LAQAGLDPVT AFPPIRVFGT VGFIVAMWAV
160 170 180 190 200
SLLHLELSSL QLYIASGASL LLSAYALTLP KIPVAEKKAT TSLASKLGLD
210 220 230 240 250
AFVLFKNPRM AIFFLFAMML GAVLQITNVF GNPFLHDFAR NPEFADSFVV
260 270 280 290 300
KYPSILLSVS QMAEVGFILT IPFFLKRFGI KTVMLMSMVA WTLRFGFFAY
310 320 330 340 350
GDPSTTGFIL LLLSMIVYGC AFDFFNISGS VFVEQEVDSS IRASAQGLFM
360 370 380 390 400
TMVNGVGAWV GSILSGMAVD YFSVDGVKDW QTIWLVFAGY ALFLAVIFFF
410
GFKYNHDPEK IKHRAVTH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 63 – 80 | DKWLR…HLVCA → VQMRARRTCIHAVSPGVC in CAA52048 (PubMed:7559336).CuratedAdd BLAST | 18 | |
Sequence conflicti | 293 – 299 | LRFGFFA → CALASSP in CAA52048 (PubMed:7559336).Curated | 7 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73828 Genomic DNA Translation: CAA52048.1 U00096 Genomic DNA Translation: AAC75459.1 AP009048 Genomic DNA Translation: BAA16274.1 |
PIRi | E65014 |
RefSeqi | NP_416901.1, NC_000913.3 WP_000020402.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75459; AAC75459; b2406 BAA16274; BAA16274; BAA16274 |
GeneIDi | 946868 |
KEGGi | ecj:JW2397 eco:b2406 |
PATRICi | fig|1411691.4.peg.4326 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73828 Genomic DNA Translation: CAA52048.1 U00096 Genomic DNA Translation: AAC75459.1 AP009048 Genomic DNA Translation: BAA16274.1 |
PIRi | E65014 |
RefSeqi | NP_416901.1, NC_000913.3 WP_000020402.1, NZ_LN832404.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGridi | 4262006, 20 interactors |
STRINGi | 511145.b2406 |
Protein family/group databases
TCDBi | 2.A.1.10.2 the major facilitator superfamily (mfs) |
Proteomic databases
jPOSTi | P45562 |
PaxDbi | P45562 |
PRIDEi | P45562 |
Genome annotation databases
EnsemblBacteriai | AAC75459; AAC75459; b2406 BAA16274; BAA16274; BAA16274 |
GeneIDi | 946868 |
KEGGi | ecj:JW2397 eco:b2406 |
PATRICi | fig|1411691.4.peg.4326 |
Organism-specific databases
EchoBASEi | EB2951 |
Phylogenomic databases
eggNOGi | ENOG4105EAA Bacteria ENOG410ZVFU LUCA |
HOGENOMi | HOG000026502 |
InParanoidi | P45562 |
KOi | K11537 |
PhylomeDBi | P45562 |
Enzyme and pathway databases
BioCyci | EcoCyc:XAPB-MONOMER ECOL316407:JW2397-MONOMER MetaCyc:XAPB-MONOMER |
Miscellaneous databases
PROi | PR:P45562 |
Family and domain databases
InterProi | View protein in InterPro IPR020846 MFS_dom IPR036259 MFS_trans_sf IPR004740 Nuc_H_symport |
Pfami | View protein in Pfam PF03825 Nuc_H_symport, 1 hit |
SUPFAMi | SSF103473 SSF103473, 1 hit |
TIGRFAMsi | TIGR00889 2A0110, 1 hit |
PROSITEi | View protein in PROSITE PS50850 MFS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | XAPB_ECOLI | |
Accessioni | P45562Primary (citable) accession number: P45562 Secondary accession number(s): P77729 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1997 | |
Last modified: | November 13, 2019 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene