UniProtKB - P45548 (PHP_ECOLI)
Phosphotriesterase homology protein
php
Functioni
Catalyzes the hydrolysis of phosphorylated glyceryl acetates in which the presence of a phosphate group is required for the enzymatic hydrolysis. Hydrolyzes a dibutyl glycerol derivative suggesting it acts on phosphoglycerol substrates with a butyrate leaving group. Also active with aromatic acetates and propionates. No activity with various sugar phosphates, with various nitrophenylphosphate or nitrophenylphosphonate derivatives, or with phosphorylated or non-phosphorylated sugar lactones tested. Does not hydrolyze non-phosphorylated carboxyesters with long chain leaving groups (PubMed:30277746).
No general esterase, aminopeptidase, sulfatase, phosphatase, carbonic anhydrase, phosphodiesterase, and phosphotriesterase activities detected when tested with the following non-specific substrates: p-nitrophenyl acetate, L-alanine nitroanilide, p-nitrophenyl sulfate, bis(p-nitrophenyl) phosphate, paraoxon, and p-nitrophenyl phosphate (PubMed:9548740).
2 PublicationsCofactori
Activity regulationi
Kineticsi
- KM=1.8 mM for 2-naphthyl acetate (in the presence of 1.0 mM MnCl2, at pH 8.0 and 30 degrees Celsius)1 Publication
- KM=5.5 mM for (2S)-1,2-diacetyl glycerol-3-phosphate (in the presence of 1.0 mM MnCl2, at pH 8.0 and 30 degrees Celsius)1 Publication
- KM=6.0 mM for rac-(2R,2S)-1,2-diacetyl glycerol-3-phosphate (in the presence of 1.0 mM MnCl2, at pH 8.0 and 30 degrees Celsius)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 12 | Zinc 1; via tele nitrogenCombined sources2 Publications | 1 | |
Metal bindingi | 14 | Zinc 1; via tele nitrogenCombined sources2 Publications | 1 | |
Metal bindingi | 125 | Zinc 1Combined sources2 Publications | 1 | |
Metal bindingi | 125 | Zinc 2Combined sources2 Publications | 1 | |
Metal bindingi | 158 | Zinc 2; via pros nitrogenCombined sources2 Publications | 1 | |
Metal bindingi | 186 | Zinc 2; via tele nitrogenCombined sources2 Publications | 1 | |
Metal bindingi | 243 | Zinc 1Combined sources2 Publications | 1 | |
Binding sitei | 280 | Beta-D-glucoseCombined sources1 Publication | 1 | |
Binding sitei | 284 | Beta-D-glucoseCombined sources1 Publication | 1 |
GO - Molecular functioni
- hydrolase activity, acting on ester bonds Source: InterPro
- zinc ion binding Source: EcoCyc
GO - Biological processi
- catabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:G7731-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:php2 Publications Synonyms:yhfV Ordered Locus Names:b3379, JW3342 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000205362 | 1 – 292 | Phosphotriesterase homology proteinAdd BLAST | 292 |
Proteomic databases
PaxDbi | P45548 |
PRIDEi | P45548 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260764, 9 interactors |
DIPi | DIP-10504N |
IntActi | P45548, 2 interactors |
STRINGi | 511145.b3379 |
Structurei
Secondary structure
3D structure databases
SMRi | P45548 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P45548 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 148 – 149 | Beta-D-glucose bindingCombined sources1 Publication | 2 | |
Regioni | 176 – 191 | Beta-D-glucose bindingCombined sources1 PublicationAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1735, Bacteria |
HOGENOMi | CLU_054760_1_1_6 |
InParanoidi | P45548 |
PhylomeDBi | P45548 |
Family and domain databases
CDDi | cd00530, PTE, 1 hit |
InterProi | View protein in InterPro IPR017947, AryldialkylPase_Zn-BS IPR032466, Metal_Hydrolase IPR001559, Phosphotriesterase |
PANTHERi | PTHR10819, PTHR10819, 1 hit |
Pfami | View protein in Pfam PF02126, PTE, 1 hit |
PIRSFi | PIRSF016839, PhP, 1 hit |
SUPFAMi | SSF51556, SSF51556, 1 hit |
PROSITEi | View protein in PROSITE PS01322, PHOSPHOTRIESTERASE_1, 1 hit PS51347, PHOSPHOTRIESTERASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSFDPTGYTL AHEHLHIDLS GFKNNVDCRL DQYAFICQEM NDLMTRGVRN
60 70 80 90 100
VIEMTNRYMG RNAQFMLDVM RETGINVVAC TGYYQDAFFP EHVATRSVQE
110 120 130 140 150
LAQEMVDEIE QGIDGTELKA GIIAEIGTSE GKITPLEEKV FIAAALAHNQ
160 170 180 190 200
TGRPISTHTS FSTMGLEQLA LLQAHGVDLS RVTVGHCDLK DNLDNILKMI
210 220 230 240 250
DLGAYVQFDT IGKNSYYPDE KRIAMLHALR DRGLLNRVML SMDITRRSHL
260 270 280 290
KANGGYGYDY LLTTFIPQLR QSGFSQADVD VMLRENPSQF FQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58176.1 U00096 Genomic DNA Translation: AAC76404.1 AP009048 Genomic DNA Translation: BAE77912.1 |
PIRi | F65132 |
RefSeqi | NP_417838.1, NC_000913.3 WP_000007010.1, NZ_SSZK01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC76404; AAC76404; b3379 BAE77912; BAE77912; BAE77912 |
GeneIDi | 947891 |
KEGGi | ecj:JW3342 eco:b3379 |
PATRICi | fig|1411691.4.peg.3351 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58176.1 U00096 Genomic DNA Translation: AAC76404.1 AP009048 Genomic DNA Translation: BAE77912.1 |
PIRi | F65132 |
RefSeqi | NP_417838.1, NC_000913.3 WP_000007010.1, NZ_SSZK01000008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BF6 | X-ray | 1.70 | A/B | 2-292 | [»] | |
4LEF | X-ray | 1.84 | A/B/C/D/E/F/G/H | 1-292 | [»] | |
SMRi | P45548 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260764, 9 interactors |
DIPi | DIP-10504N |
IntActi | P45548, 2 interactors |
STRINGi | 511145.b3379 |
Proteomic databases
PaxDbi | P45548 |
PRIDEi | P45548 |
Genome annotation databases
EnsemblBacteriai | AAC76404; AAC76404; b3379 BAE77912; BAE77912; BAE77912 |
GeneIDi | 947891 |
KEGGi | ecj:JW3342 eco:b3379 |
PATRICi | fig|1411691.4.peg.3351 |
Organism-specific databases
EchoBASEi | EB2753 |
Phylogenomic databases
eggNOGi | COG1735, Bacteria |
HOGENOMi | CLU_054760_1_1_6 |
InParanoidi | P45548 |
PhylomeDBi | P45548 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7731-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P45548 |
PROi | PR:P45548 |
Family and domain databases
CDDi | cd00530, PTE, 1 hit |
InterProi | View protein in InterPro IPR017947, AryldialkylPase_Zn-BS IPR032466, Metal_Hydrolase IPR001559, Phosphotriesterase |
PANTHERi | PTHR10819, PTHR10819, 1 hit |
Pfami | View protein in Pfam PF02126, PTE, 1 hit |
PIRSFi | PIRSF016839, PhP, 1 hit |
SUPFAMi | SSF51556, SSF51556, 1 hit |
PROSITEi | View protein in PROSITE PS01322, PHOSPHOTRIESTERASE_1, 1 hit PS51347, PHOSPHOTRIESTERASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PHP_ECOLI | |
Accessioni | P45548Primary (citable) accession number: P45548 Secondary accession number(s): Q2M744 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | June 2, 2021 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families