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UniProtKB - P45543 (FRLD_ECOLI)
Protein
Fructoselysine 6-kinase
Gene
frlD
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the ATP-dependent phosphorylation of fructoselysine to fructoselysine 6-phosphate (PubMed:12147680).
Functions in a fructoselysine degradation pathway that allows E.coli to grow on fructoselysine or psicoselysine (PubMed:12147680, PubMed:14641112).
To a much lesser extenst, is also able to phosphorylate psicoselysine (PubMed:14641112).
2 PublicationsCatalytic activityi
- EC:2.7.1.2181 Publication
Kineticsi
- KM=18 µM for fructoselysine1 Publication
- KM=50 µM for ATP1 Publication
- KM=10 mM for psicoselysine1 Publication
- Vmax=30 µmol/min/mg enzyme with fructoselysine as substrate1 Publication
- Vmax=9 µmol/min/mg enzyme with psicoselysine as substrate1 Publication
: fructoselysine degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes D-glucose 6-phosphate and lysine from fructoselysine.2 Publications This subpathway is part of the pathway fructoselysine degradation, which is itself part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucose 6-phosphate and lysine from fructoselysine, the pathway fructoselysine degradation and in Carbohydrate metabolism.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- carbohydrate kinase activity Source: EcoCyc
- phosphotransferase activity, alcohol group as acceptor Source: InterPro
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G7726-MONOMER |
BRENDAi | 2.7.1.218, 2026 |
SABIO-RKi | P45543 |
UniPathwayi | UPA00784;UER00769 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:frlD1 Publication Synonyms:yhfQ Ordered Locus Names:b3374, JW3337 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080066 | 1 – 261 | Fructoselysine 6-kinaseAdd BLAST | 261 |
Proteomic databases
PaxDbi | P45543 |
Expressioni
Inductioni
Induced by fructoselysine and psicoselysine. Makes part of the frl operon with FrlA, FrlB, FrlC and FrlR.2 Publications
Interactioni
Subunit structurei
Monomer.
1 PublicationBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 4262482, 9 interactors 852195, 5 interactors |
DIPi | DIP-12326N |
IntActi | P45543, 9 interactors |
STRINGi | 511145.b3374 |
Family & Domainsi
Sequence similaritiesi
Belongs to the carbohydrate kinase PfkB family.Curated
Phylogenomic databases
eggNOGi | COG0524, Bacteria |
HOGENOMi | CLU_027634_13_0_6 |
InParanoidi | P45543 |
PhylomeDBi | P45543 |
Family and domain databases
Gene3Di | 3.40.1190.20, 1 hit |
InterProi | View protein in InterPro IPR002173, Carboh/pur_kinase_PfkB_CS IPR011611, PfkB_dom IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit |
PROSITEi | View protein in PROSITE PS00583, PFKB_KINASES_1, 1 hit PS00584, PFKB_KINASES_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P45543-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKTLATIGDN CVDIYPQLNK AFSGGNAVNV AVYCTRYGIQ PGCITWVGDD
60 70 80 90 100
DYGTKLKQDL ARMGVDISHV HTKHGVTAQT QVELHDNDRV FGDYTEGVMA
110 120 130 140 150
DFALSEEDYA WLAQYDIVHA AIWGHAEDAF PQLHAAGKLT AFDFSDKWDS
160 170 180 190 200
PLWQTLVPHL DFAFASAPQE DETLRLKMKA IVARGAGTVI VTLGENGSIA
210 220 230 240 250
WDGAQFWRQA PEPVTVIDTM GAGDSFIAGF LCGWSAGMTL PQAIAQGTAC
260
AAKTIQYHGA W
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58171.1 U00096 Genomic DNA Translation: AAC76399.1 AP009048 Genomic DNA Translation: BAE77917.1 |
PIRi | A65132 |
RefSeqi | NP_417833.1, NC_000913.3 WP_000853353.1, NZ_SSZK01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC76399; AAC76399; b3374 BAE77917; BAE77917; BAE77917 |
GeneIDi | 947886 |
KEGGi | ecj:JW3337 eco:b3374 |
PATRICi | fig|511145.12.peg.3467 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58171.1 U00096 Genomic DNA Translation: AAC76399.1 AP009048 Genomic DNA Translation: BAE77917.1 |
PIRi | A65132 |
RefSeqi | NP_417833.1, NC_000913.3 WP_000853353.1, NZ_SSZK01000008.1 |
3D structure databases
SMRi | P45543 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262482, 9 interactors 852195, 5 interactors |
DIPi | DIP-12326N |
IntActi | P45543, 9 interactors |
STRINGi | 511145.b3374 |
Proteomic databases
PaxDbi | P45543 |
Genome annotation databases
EnsemblBacteriai | AAC76399; AAC76399; b3374 BAE77917; BAE77917; BAE77917 |
GeneIDi | 947886 |
KEGGi | ecj:JW3337 eco:b3374 |
PATRICi | fig|511145.12.peg.3467 |
Organism-specific databases
EchoBASEi | EB2748 |
Phylogenomic databases
eggNOGi | COG0524, Bacteria |
HOGENOMi | CLU_027634_13_0_6 |
InParanoidi | P45543 |
PhylomeDBi | P45543 |
Enzyme and pathway databases
UniPathwayi | UPA00784;UER00769 |
BioCyci | EcoCyc:G7726-MONOMER |
BRENDAi | 2.7.1.218, 2026 |
SABIO-RKi | P45543 |
Miscellaneous databases
PROi | PR:P45543 |
Family and domain databases
Gene3Di | 3.40.1190.20, 1 hit |
InterProi | View protein in InterPro IPR002173, Carboh/pur_kinase_PfkB_CS IPR011611, PfkB_dom IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit |
PROSITEi | View protein in PROSITE PS00583, PFKB_KINASES_1, 1 hit PS00584, PFKB_KINASES_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FRLD_ECOLI | |
Accessioni | P45543Primary (citable) accession number: P45543 Secondary accession number(s): Q2M739 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families