UniProtKB - P45531 (TUSC_ECOLI)
Protein
Protein TusC
Gene
tusC
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions.1 Publication
GO - Biological processi
- tRNA wobble position uridine thiolation Source: EcoCyc
Keywordsi
Biological process | tRNA processing |
Enzyme and pathway databases
BioCyci | EcoCyc:G7713-MONOMER MetaCyc:G7713-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Protein TusCAlternative name(s): tRNA 2-thiouridine synthesizing protein C |
Gene namesi | Name:tusC Synonyms:yheM Ordered Locus Names:b3344, JW3306 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- sulfurtransferase complex Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 76 | C → S: No effect. 1 Publication | 1 | |
Mutagenesisi | 79 | C → S: No effect. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000214883 | 1 – 119 | Protein TusCAdd BLAST | 119 |
Proteomic databases
PaxDbi | P45531 |
PRIDEi | P45531 |
Interactioni
Subunit structurei
Heterohexamer, formed by a dimer of trimers. The hexameric TusBCD complex contains 2 copies each of TusB, TusC and TusD. The TusBCD complex interacts with TusE.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4262468, 28 interactors |
ComplexPortali | CPX-2144, TusBCDE complex |
DIPi | DIP-12309N |
IntActi | P45531, 7 interactors |
STRINGi | 511145.b3344 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P45531 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P45531 |
Family & Domainsi
Sequence similaritiesi
Belongs to the DsrF/TusC family.Curated
Phylogenomic databases
eggNOGi | COG2923, Bacteria |
HOGENOMi | CLU_155943_1_0_6 |
InParanoidi | P45531 |
Family and domain databases
Gene3Di | 3.40.1260.10, 1 hit |
HAMAPi | MF_00389, Thiourid_synth_C, 1 hit |
InterProi | View protein in InterPro IPR027396, DsrEFH-like IPR003787, Sulphur_relay_DrsE/F-like IPR037450, Sulphur_relay_TusC IPR017462, Sulphur_relay_TusC/DsrF |
PANTHERi | PTHR38780, PTHR38780, 1 hit |
Pfami | View protein in Pfam PF02635, DrsE, 1 hit |
SUPFAMi | SSF75169, SSF75169, 1 hit |
TIGRFAMsi | TIGR03010, sulf_tusC_dsrF, 1 hit |
i Sequence
Sequence statusi: Complete.
P45531-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKRIAFVFST APHGTAAGRE GLDALLATSA LTDDLAVFFI ADGVFQLLPG
60 70 80 90 100
QKPDAVLARD YIATFKLLGL YDIEQCWVCA ASLRERGLDP QTPFVVEATP
110
LEADALRREL ANYDVILRF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58141.1 U00096 Genomic DNA Translation: AAC76369.1 AP009048 Genomic DNA Translation: BAE77947.1 |
PIRi | C65128 |
RefSeqi | NP_417803.1, NC_000913.3 WP_000820714.1, NZ_SSZK01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC76369; AAC76369; b3344 BAE77947; BAE77947; BAE77947 |
GeneIDi | 947853 |
KEGGi | ecj:JW3306 eco:b3344 |
PATRICi | fig|1411691.4.peg.3387 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58141.1 U00096 Genomic DNA Translation: AAC76369.1 AP009048 Genomic DNA Translation: BAE77947.1 |
PIRi | C65128 |
RefSeqi | NP_417803.1, NC_000913.3 WP_000820714.1, NZ_SSZK01000008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2D1P | X-ray | 2.15 | B/E/H | 1-119 | [»] | |
SMRi | P45531 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262468, 28 interactors |
ComplexPortali | CPX-2144, TusBCDE complex |
DIPi | DIP-12309N |
IntActi | P45531, 7 interactors |
STRINGi | 511145.b3344 |
Proteomic databases
PaxDbi | P45531 |
PRIDEi | P45531 |
Genome annotation databases
EnsemblBacteriai | AAC76369; AAC76369; b3344 BAE77947; BAE77947; BAE77947 |
GeneIDi | 947853 |
KEGGi | ecj:JW3306 eco:b3344 |
PATRICi | fig|1411691.4.peg.3387 |
Organism-specific databases
EchoBASEi | EB2734 |
Phylogenomic databases
eggNOGi | COG2923, Bacteria |
HOGENOMi | CLU_155943_1_0_6 |
InParanoidi | P45531 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7713-MONOMER MetaCyc:G7713-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P45531 |
PROi | PR:P45531 |
Family and domain databases
Gene3Di | 3.40.1260.10, 1 hit |
HAMAPi | MF_00389, Thiourid_synth_C, 1 hit |
InterProi | View protein in InterPro IPR027396, DsrEFH-like IPR003787, Sulphur_relay_DrsE/F-like IPR037450, Sulphur_relay_TusC IPR017462, Sulphur_relay_TusC/DsrF |
PANTHERi | PTHR38780, PTHR38780, 1 hit |
Pfami | View protein in Pfam PF02635, DrsE, 1 hit |
SUPFAMi | SSF75169, SSF75169, 1 hit |
TIGRFAMsi | TIGR03010, sulf_tusC_dsrF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TUSC_ECOLI | |
Accessioni | P45531Primary (citable) accession number: P45531 Secondary accession number(s): Q2M709 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families