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UniProtKB - P45522 (KEFB_ECOLI)

Entry version 165 (25 May 2022)
Sequence version 1 (01 Nov 1995)
Previous versions | rss
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Protein

Glutathione-regulated potassium-efflux system protein KefB

Gene

kefB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Pore-forming subunit of a potassium efflux system that confers protection against electrophiles. Catalyzes K+/H+ antiport.

UniRule annotation2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Activated by adducts between glutathione and electrophiles.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

  • potassium ion transport Source: EcoliWiki
  • regulation of intracellular pH Source: ComplexPortal
Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processAntiport, Ion transport, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:KEFB-MONOMER

Protein family/group databases

Transport Classification Database

More...TCDBi		2.A.37.1.2, the monovalent cation:proton antiporter-2 (cpa2) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Glutathione-regulated potassium-efflux system protein KefBUniRule annotation
Alternative name(s):
K(+)/H(+) antiporterUniRule annotation
NEM-activable K(+)/H(+) antiporter
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:kefBUniRule annotation
Synonyms:trkB
Ordered Locus Names:b3350, JW3313
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei4 – 24HelicalUniRule annotationAdd BLAST21
Transmembranei29 – 49HelicalUniRule annotationAdd BLAST21
Transmembranei55 – 75HelicalUniRule annotationAdd BLAST21
Transmembranei87 – 107HelicalUniRule annotationAdd BLAST21
Transmembranei115 – 135HelicalUniRule annotationAdd BLAST21
Transmembranei152 – 172HelicalUniRule annotationAdd BLAST21
Transmembranei177 – 197HelicalUniRule annotationAdd BLAST21
Transmembranei207 – 227HelicalUniRule annotationAdd BLAST21
Transmembranei230 – 250HelicalUniRule annotationAdd BLAST21
Transmembranei268 – 288HelicalUniRule annotationAdd BLAST21
Transmembranei291 – 311HelicalUniRule annotationAdd BLAST21
Transmembranei324 – 344HelicalUniRule annotationAdd BLAST21
Transmembranei356 – 376HelicalUniRule annotationAdd BLAST21

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001965961 – 601Glutathione-regulated potassium-efflux system protein KefBAdd BLAST601

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P45522

PRoteomics IDEntifications database

More...PRIDEi		P45522

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with the regulatory subunit KefG.

UniRule annotation

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4262061, 18 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-3090, Glutathione-regulated potassium-efflux system KefB-KefG complex

STRING: functional protein association networks

More...STRINGi		511145.b3350

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		P45522

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P45522

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini402 – 524RCK N-terminalUniRule annotationAdd BLAST123

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. KefB subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0475, Bacteria
COG1226, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_005126_9_3_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P45522

Identification of Orthologs from Complete Genome Data

More...OMAi		IRPFHDV

Database for complete collections of gene phylogenies

More...PhylomeDBi		P45522

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.20.1530.20, 1 hit

HAMAP database of protein families

More...HAMAPi		MF_01412, K_H_efflux_KefB, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR006153, Cation/H_exchanger
IPR004771, K/H_exchanger
IPR020884, K_H_efflux_KefB
IPR038770, Na+/solute_symporter_sf
IPR036291, NAD(P)-bd_dom_sf
IPR003148, RCK_N

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00999, Na_H_Exchanger, 1 hit
PF02254, TrkA_N, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF51735, SSF51735, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00932, 2a37, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51201, RCK_N, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P45522-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEGSDFLLAG VLFLFAAVAA VPLASRLGIG AVLGYLLAGI AIGPWGLGFI 
        60         70         80         90        100
SDVDEILHFS ELGVVFLMFI IGLELNPSKL WQLRRSIFGV GAAQVLLSAA 
       110        120        130        140        150
LLAGLLMLTD FAWQAAVVGG IGLAMSSTAM ALQLMREKGM NRSESGQLGF 
       160        170        180        190        200
SVLLFQDLAV IPALALVPLL AGSADEHFDW MKVGMKVLAF VGMLIGGRYL 
       210        220        230        240        250
LRPVFRFIAA SGVREVFTAA TLLLVLGSAL FMDALGLSMA LGTFIAGVLL 
       260        270        280        290        300
AESEYRHELE TAIDPFKGLL LGLFFISVGM SLNLGVLYTH LLWVVISVVV 
       310        320        330        340        350
LVAVKILVLY LLARLYGVRS SERMQFAGVL SQGGEFAFVL FSTASSQRLF 
       360        370        380        390        400
QGDQMALLLV TVTLSMMTTP LLMKLVDKWL SRQFNGPEEE DEKPWVNDDK 
       410        420        430        440        450
PQVIVVGFGR FGQVIGRLLM ANKMRITVLE RDISAVNLMR KYGYKVYYGD 
       460        470        480        490        500
ATQVDLLRSA GAEAAESIVI TCNEPEDTMK LVEICQQHFP HLHILARARG 
       510        520        530        540        550
RVEAHELLQA GVTQFSRETF SSALELGRKT LVTLGMHPHQ AQRAQLHFRR 
       560        570        580        590        600
LDMRMLRELI PMHADTVQIS RAREARRELE EIFQREMQQE RRQLDGWDEF 

E
Length:601
Mass (Da):66,411
Last modified:November 1, 1995 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB7534C7F4541497E
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA58147.1
U00096 Genomic DNA Translation: AAC76375.1
AP009048 Genomic DNA Translation: BAE77940.1

Protein sequence database of the Protein Information Resource

More...PIRi		A65129

NCBI Reference Sequences

More...RefSeqi		NP_417809.1, NC_000913.3
WP_000399147.1, NZ_SSZK01000008.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC76375; AAC76375; b3350
BAE77940; BAE77940; BAE77940

Database of genes from NCBI RefSeq genomes

More...GeneIDi		947858

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW3313
eco:b3350

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.3380

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA58147.1
U00096 Genomic DNA Translation: AAC76375.1
AP009048 Genomic DNA Translation: BAE77940.1
PIRiA65129
RefSeqiNP_417809.1, NC_000913.3
WP_000399147.1, NZ_SSZK01000008.1

3D structure databases

AlphaFoldDBiP45522
SMRiP45522
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4262061, 18 interactors
ComplexPortaliCPX-3090, Glutathione-regulated potassium-efflux system KefB-KefG complex
STRINGi511145.b3350

Protein family/group databases

TCDBi2.A.37.1.2, the monovalent cation:proton antiporter-2 (cpa2) family

Proteomic databases

PaxDbiP45522
PRIDEiP45522

Genome annotation databases

EnsemblBacteriaiAAC76375; AAC76375; b3350
BAE77940; BAE77940; BAE77940
GeneIDi947858
KEGGiecj:JW3313
eco:b3350
PATRICifig|1411691.4.peg.3380

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB4144

Phylogenomic databases

eggNOGiCOG0475, Bacteria
COG1226, Bacteria
HOGENOMiCLU_005126_9_3_6
InParanoidiP45522
OMAiIRPFHDV
PhylomeDBiP45522

Enzyme and pathway databases

BioCyciEcoCyc:KEFB-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P45522

Family and domain databases

Gene3Di1.20.1530.20, 1 hit
HAMAPiMF_01412, K_H_efflux_KefB, 1 hit
InterProiView protein in InterPro
IPR006153, Cation/H_exchanger
IPR004771, K/H_exchanger
IPR020884, K_H_efflux_KefB
IPR038770, Na+/solute_symporter_sf
IPR036291, NAD(P)-bd_dom_sf
IPR003148, RCK_N
PfamiView protein in Pfam
PF00999, Na_H_Exchanger, 1 hit
PF02254, TrkA_N, 1 hit
SUPFAMiSSF51735, SSF51735, 1 hit
TIGRFAMsiTIGR00932, 2a37, 1 hit
PROSITEiView protein in PROSITE
PS51201, RCK_N, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiKEFB_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P45522
Secondary accession number(s): Q2M716
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 25, 2022
This is version 165 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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