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UniProtKB - P45522 (KEFB_ECOLI)
Protein
Glutathione-regulated potassium-efflux system protein KefB
Gene
kefB
Organism
Escherichia coli (strain K12)
Status
Functioni
Pore-forming subunit of a potassium efflux system that confers protection against electrophiles. Catalyzes K+/H+ antiport.
UniRule annotation2 PublicationsActivity regulationi
Activated by adducts between glutathione and electrophiles.1 Publication
GO - Molecular functioni
- glutathione-regulated potassium exporter activity Source: UniProtKB-UniRule
- solute:proton antiporter activity Source: InterPro
GO - Biological processi
- potassium ion transport Source: EcoliWiki
- regulation of intracellular pH Source: ComplexPortal
Keywordsi
Biological process | Antiport, Ion transport, Potassium transport, Transport |
Ligand | Potassium |
Enzyme and pathway databases
BioCyci | EcoCyc:KEFB-MONOMER |
Protein family/group databases
TCDBi | 2.A.37.1.2, the monovalent cation:proton antiporter-2 (cpa2) family |
Names & Taxonomyi
Protein namesi | Recommended name: Glutathione-regulated potassium-efflux system protein KefBUniRule annotationAlternative name(s): K(+)/H(+) antiporterUniRule annotation NEM-activable K(+)/H(+) antiporter |
Gene namesi | Name:kefBUniRule annotation Synonyms:trkB Ordered Locus Names:b3350, JW3313 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation1 Publication
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: EcoCyc
Other locations
- membrane Source: GO_Central
- potassium:proton antiporter complex Source: ComplexPortal
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 4 – 24 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 29 – 49 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 55 – 75 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 87 – 107 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 115 – 135 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 152 – 172 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 177 – 197 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 207 – 227 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 230 – 250 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 268 – 288 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 291 – 311 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 324 – 344 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 356 – 376 | HelicalUniRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000196596 | 1 – 601 | Glutathione-regulated potassium-efflux system protein KefBAdd BLAST | 601 |
Proteomic databases
PaxDbi | P45522 |
PRIDEi | P45522 |
Interactioni
Subunit structurei
Interacts with the regulatory subunit KefG.
UniRule annotationProtein-protein interaction databases
BioGRIDi | 4262061, 18 interactors |
ComplexPortali | CPX-3090, Glutathione-regulated potassium-efflux system KefB-KefG complex |
STRINGi | 511145.b3350 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 402 – 524 | RCK N-terminalUniRule annotationAdd BLAST | 123 |
Sequence similaritiesi
Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. KefB subfamily. [View classification]UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0475, Bacteria COG1226, Bacteria |
HOGENOMi | CLU_005126_9_3_6 |
InParanoidi | P45522 |
OMAi | IRPFHDV |
PhylomeDBi | P45522 |
Family and domain databases
Gene3Di | 1.20.1530.20, 1 hit |
HAMAPi | MF_01412, K_H_efflux_KefB, 1 hit |
InterProi | View protein in InterPro IPR006153, Cation/H_exchanger IPR004771, K/H_exchanger IPR020884, K_H_efflux_KefB IPR038770, Na+/solute_symporter_sf IPR036291, NAD(P)-bd_dom_sf IPR003148, RCK_N |
Pfami | View protein in Pfam PF00999, Na_H_Exchanger, 1 hit PF02254, TrkA_N, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00932, 2a37, 1 hit |
PROSITEi | View protein in PROSITE PS51201, RCK_N, 1 hit |
i Sequence
Sequence statusi: Complete.
P45522-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEGSDFLLAG VLFLFAAVAA VPLASRLGIG AVLGYLLAGI AIGPWGLGFI
60 70 80 90 100
SDVDEILHFS ELGVVFLMFI IGLELNPSKL WQLRRSIFGV GAAQVLLSAA
110 120 130 140 150
LLAGLLMLTD FAWQAAVVGG IGLAMSSTAM ALQLMREKGM NRSESGQLGF
160 170 180 190 200
SVLLFQDLAV IPALALVPLL AGSADEHFDW MKVGMKVLAF VGMLIGGRYL
210 220 230 240 250
LRPVFRFIAA SGVREVFTAA TLLLVLGSAL FMDALGLSMA LGTFIAGVLL
260 270 280 290 300
AESEYRHELE TAIDPFKGLL LGLFFISVGM SLNLGVLYTH LLWVVISVVV
310 320 330 340 350
LVAVKILVLY LLARLYGVRS SERMQFAGVL SQGGEFAFVL FSTASSQRLF
360 370 380 390 400
QGDQMALLLV TVTLSMMTTP LLMKLVDKWL SRQFNGPEEE DEKPWVNDDK
410 420 430 440 450
PQVIVVGFGR FGQVIGRLLM ANKMRITVLE RDISAVNLMR KYGYKVYYGD
460 470 480 490 500
ATQVDLLRSA GAEAAESIVI TCNEPEDTMK LVEICQQHFP HLHILARARG
510 520 530 540 550
RVEAHELLQA GVTQFSRETF SSALELGRKT LVTLGMHPHQ AQRAQLHFRR
560 570 580 590 600
LDMRMLRELI PMHADTVQIS RAREARRELE EIFQREMQQE RRQLDGWDEF
E
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58147.1 U00096 Genomic DNA Translation: AAC76375.1 AP009048 Genomic DNA Translation: BAE77940.1 |
PIRi | A65129 |
RefSeqi | NP_417809.1, NC_000913.3 WP_000399147.1, NZ_SSZK01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC76375; AAC76375; b3350 BAE77940; BAE77940; BAE77940 |
GeneIDi | 947858 |
KEGGi | ecj:JW3313 eco:b3350 |
PATRICi | fig|1411691.4.peg.3380 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58147.1 U00096 Genomic DNA Translation: AAC76375.1 AP009048 Genomic DNA Translation: BAE77940.1 |
PIRi | A65129 |
RefSeqi | NP_417809.1, NC_000913.3 WP_000399147.1, NZ_SSZK01000008.1 |
3D structure databases
AlphaFoldDBi | P45522 |
SMRi | P45522 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262061, 18 interactors |
ComplexPortali | CPX-3090, Glutathione-regulated potassium-efflux system KefB-KefG complex |
STRINGi | 511145.b3350 |
Protein family/group databases
TCDBi | 2.A.37.1.2, the monovalent cation:proton antiporter-2 (cpa2) family |
Proteomic databases
PaxDbi | P45522 |
PRIDEi | P45522 |
Genome annotation databases
EnsemblBacteriai | AAC76375; AAC76375; b3350 BAE77940; BAE77940; BAE77940 |
GeneIDi | 947858 |
KEGGi | ecj:JW3313 eco:b3350 |
PATRICi | fig|1411691.4.peg.3380 |
Organism-specific databases
EchoBASEi | EB4144 |
Phylogenomic databases
eggNOGi | COG0475, Bacteria COG1226, Bacteria |
HOGENOMi | CLU_005126_9_3_6 |
InParanoidi | P45522 |
OMAi | IRPFHDV |
PhylomeDBi | P45522 |
Enzyme and pathway databases
BioCyci | EcoCyc:KEFB-MONOMER |
Miscellaneous databases
PROi | PR:P45522 |
Family and domain databases
Gene3Di | 1.20.1530.20, 1 hit |
HAMAPi | MF_01412, K_H_efflux_KefB, 1 hit |
InterProi | View protein in InterPro IPR006153, Cation/H_exchanger IPR004771, K/H_exchanger IPR020884, K_H_efflux_KefB IPR038770, Na+/solute_symporter_sf IPR036291, NAD(P)-bd_dom_sf IPR003148, RCK_N |
Pfami | View protein in Pfam PF00999, Na_H_Exchanger, 1 hit PF02254, TrkA_N, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR00932, 2a37, 1 hit |
PROSITEi | View protein in PROSITE PS51201, RCK_N, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KEFB_ECOLI | |
Accessioni | P45522Primary (citable) accession number: P45522 Secondary accession number(s): Q2M716 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families