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UniProtKB - P45510 (DHAK_CITFR)
Protein
Dihydroxyacetone kinase
Gene
dhaK
Organism
Citrobacter freundii
Status
Functioni
Catalyzes the phosphorylation of dihydroxyacetone.
Catalytic activityi
- EC:2.7.1.292 Publications
Cofactori
Kineticsi
- KM=30 µM for dihydroxyacetone1 Publication
- KM=70 µM for ATP1 Publication
: glycerol fermentation Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route). This subpathway is part of the pathway glycerol fermentation, which is itself part of Polyol metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from glycerol (oxidative route), the pathway glycerol fermentation and in Polyol metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 109 | Substrate | 1 | |
Binding sitei | 114 | Substrate | 1 | |
Active sitei | 220 | Tele-hemiaminal-histidine intermediatePROSITE-ProRule annotation1 Publication | 1 | |
Binding sitei | 468 | ATP; via carbonyl oxygen1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 385 – 388 | ATP1 Publication | 4 | |
Nucleotide bindingi | 431 – 432 | ATP1 Publication | 2 | |
Nucleotide bindingi | 476 – 477 | ATP1 Publication | 2 | |
Nucleotide bindingi | 533 – 535 | ATP1 Publication | 3 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glycerone kinase activity Source: UniProtKB-EC
- lipid binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- anaerobic glycerol catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Glycerol metabolism |
Ligand | ATP-binding, Calcium, Lipid-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.1.29, 1398 |
SABIO-RKi | P45510 |
UniPathwayi | UPA00617;UER00669 |
Names & Taxonomyi
Protein namesi | Recommended name: Dihydroxyacetone kinase (EC:2.7.1.29)Short name: DHA kinase Alternative name(s): Glycerone kinase |
Gene namesi | Name:dhaK |
Organismi | Citrobacter freundii |
Taxonomic identifieri | 546 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Citrobacter › Citrobacter freundii complex |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 380 | D → A: Loss of kinase activity. 1 Publication | 1 | |
Mutagenesisi | 385 | D → A: Loss of kinase activity. 1 Publication | 1 | |
Mutagenesisi | 387 | D → A: Loss of kinase activity. 1 Publication | 1 | |
Mutagenesisi | 388 | T → H: Reduced kinase activity. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB01775, Dihydroxyacetone DB04395, Phosphoaminophosphonic Acid-Adenylate Ester |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000121528 | 2 – 552 | Dihydroxyacetone kinaseAdd BLAST | 551 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
STRINGi | 1333848.CFNIH1_02650 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P45510 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P45510 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 327 | DhaKPROSITE-ProRule annotationAdd BLAST | 320 | |
Domaini | 356 – 548 | DhaLPROSITE-ProRule annotationAdd BLAST | 193 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 58 – 61 | Substrate binding | 4 |
Domaini
The C-terminal domain consists of a eight-helix alpha barrel. The eight helices form a pocket that includes a tightly bound phospholipid. The cellular function of this lipid is unknown; it has no significant effect on enzyme activity.
Family and domain databases
Gene3Di | 1.25.40.340, 1 hit |
InterProi | View protein in InterPro IPR012734, DhaK_ATP IPR004006, DhaK_dom IPR004007, DhaL_dom IPR036117, DhaL_dom_sf |
Pfami | View protein in Pfam PF02733, Dak1, 1 hit PF02734, Dak2, 1 hit |
SMARTi | View protein in SMART SM01120, Dak2, 1 hit |
SUPFAMi | SSF101473, SSF101473, 1 hit |
TIGRFAMsi | TIGR02361, dak_ATP, 1 hit |
PROSITEi | View protein in PROSITE PS51481, DHAK, 1 hit PS51480, DHAL, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P45510-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQFFFNQRT HLVSDVIDGA IIASPWNNLA RLESDPAIRI VVRRDLNKNN
60 70 80 90 100
VAVISGGGSG HEPAHVGFIG KGMLTAAVCG DVFASPSVDA VLTAIQAVTG
110 120 130 140 150
EAGCLLIVKN YTGDRLNFGL AAEKARRLGY NVEMLIVGDD ISLPDNKHPR
160 170 180 190 200
GIAGTILVHK IAGYFAERGY NLATVLREAQ YAASNTFSLG VALSSCHLPQ
210 220 230 240 250
ETDAAPRHHP GHAELGMGIH GEPGASVIDT QNSAQVVNLM VDKLLAALPE
260 270 280 290 300
TGRLAVMINN LGGVSVAEMA IITRELASSP LHSRIDWLIG PASLVTALDM
310 320 330 340 350
KGFSLTAIVL EESIEKALLT EVETSNWPTP VPPREITCVV SSHASARVEF
360 370 380 390 400
QPSANALVAG IVELVTATLS DLETHLNALD AKVGDGDTGS TFAAAAREIA
410 420 430 440 450
SLLHRQQLPL NNLATLFALI GERLTVVMGG SSGVLMSIFF TAAGQKLEQG
460 470 480 490 500
ANVVEALNTG LAQMKFYGGA DEGDRTMIDA LQPALTSLLA QPKNLQAAFD
510 520 530 540 550
AAQAGAERTC LSSKANAGRA SYLSSESLLG NMDPGAQRLA MVFKALAESE
LG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09771 Genomic DNA Translation: AAB48843.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09771 Genomic DNA Translation: AAB48843.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1UN8 | X-ray | 2.50 | A/B | 1-552 | [»] | |
1UN9 | X-ray | 3.10 | A/B | 2-552 | [»] | |
SMRi | P45510 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 1333848.CFNIH1_02650 |
Chemistry databases
DrugBanki | DB01775, Dihydroxyacetone DB04395, Phosphoaminophosphonic Acid-Adenylate Ester |
Enzyme and pathway databases
UniPathwayi | UPA00617;UER00669 |
BRENDAi | 2.7.1.29, 1398 |
SABIO-RKi | P45510 |
Miscellaneous databases
EvolutionaryTracei | P45510 |
Family and domain databases
Gene3Di | 1.25.40.340, 1 hit |
InterProi | View protein in InterPro IPR012734, DhaK_ATP IPR004006, DhaK_dom IPR004007, DhaL_dom IPR036117, DhaL_dom_sf |
Pfami | View protein in Pfam PF02733, Dak1, 1 hit PF02734, Dak2, 1 hit |
SMARTi | View protein in SMART SM01120, Dak2, 1 hit |
SUPFAMi | SSF101473, SSF101473, 1 hit |
TIGRFAMsi | TIGR02361, dak_ATP, 1 hit |
PROSITEi | View protein in PROSITE PS51481, DHAK, 1 hit PS51480, DHAL, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHAK_CITFR | |
Accessioni | P45510Primary (citable) accession number: P45510 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 92 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references