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Protein

Palmitoyl-protein thioesterase 1

Gene

PPT1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.1 Publication

Catalytic activityi

Palmitoyl-[protein] + H2O = palmitate + [protein].1 Publication

Kineticsi

  1. KM=5.7 µM for palmitoyl-CoA1 Publication
  2. KM=2.3 µM for stearoyl-CoA1 Publication
  3. KM=2.8 µM for oleoyl-CoA1 Publication
  4. KM=7.5 µM for myristoyl-CoA1 Publication
  1. Vmax=5.7 nmol/min/mg enzyme with palmitoyl-CoA as substrate1 Publication
  2. Vmax=1.8 nmol/min/mg enzyme with stearoyl-CoA as substrate1 Publication
  3. Vmax=1.7 nmol/min/mg enzyme with oleoyl-CoA as substrate1 Publication
  4. Vmax=1.9 nmol/min/mg enzyme with myristoyl-CoA as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1151 Publication1
Active sitei2331 Publication1
Active sitei2891 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Protein family/group databases

ESTHERibovin-ppt Palmitoyl-protein_thioesterase

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyl-protein thioesterase 1 (EC:3.1.2.22)
Short name:
PPT-1
Alternative name(s):
Palmitoyl-protein hydrolase 1
Gene namesi
Name:PPT1
Synonyms:CLN1, PPT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
ChainiPRO_000002554928 – 306Palmitoyl-protein thioesterase 1Add BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi45 ↔ 46By similarity
Disulfide bondi96 ↔ 128By similarity
Disulfide bondi152 ↔ 160By similarity
Glycosylationi197N-linked (GlcNAc...) asparagine1
Glycosylationi212N-linked (GlcNAc...) asparagine1
Glycosylationi232N-linked (GlcNAc...) asparagine1

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP45478
PRIDEiP45478

Expressioni

Tissue specificityi

Spleen, brain, seminal vesicle, and testis. Lower levels of activity in liver, heart, lung, and skeletal muscle.1 Publication

Interactioni

Subunit structurei

Interacts with CLN5, ATP5F1A and ATP5F1B.By similarity

Protein-protein interaction databases

IntActiP45478, 2 interactors
STRINGi9913.ENSBTAP00000017780

Structurei

Secondary structure

1306
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP45478
SMRiP45478
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45478

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2541 Eukaryota
COG1075 LUCA
HOGENOMiHOG000199232
HOVERGENiHBG018186
InParanoidiP45478
KOiK01074

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002472 Palm_thioest
IPR030294 PPT1
PANTHERiPTHR11247:SF8 PTHR11247:SF8, 1 hit
PfamiView protein in Pfam
PF02089 Palm_thioest, 1 hit
PRINTSiPR00414 PPTHIESTRASE
SUPFAMiSSF53474 SSF53474, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

P45478-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASSSCLWLL ALAFLLGSCA SLALGHLDPP APLPLVIWHG MGDSCCNPLS
60 70 80 90 100
MGAIKKMVEK KIPGIHVLSL EIGKTLREDV ENSFFLNVNS QVTTVCQILA
110 120 130 140 150
KDPKLQQGYN AMGFSQGGQF LRAVAQRCPS PPMVNLISVG GQHQGVFGLP
160 170 180 190 200
RCPGESSHIC DFIRKTLNAG AYNKAIQERL VQAEYWHDPI REDIYRNHSI
210 220 230 240 250
FLADINQERG VNESYKKNLM ALKKFVMVKF LNDTIVDPVD SEWFGFYRSG
260 270 280 290 300
QAKETIPLQE STLYTQDRLG LKAMDKAGQL VFLALEGDHL QLSEEWFYAH

IIPFLE
Length:306
Mass (Da):34,142
Last modified:November 1, 1995 - v1
Checksum:iF9893D3CE8099D51
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F1MSA1F1MSA1_BOVIN
Palmitoyl-protein thioesterase 1
PPT1
306Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti72I → F in clone BOVPTT-25. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34261 mRNA Translation: AAA59357.1
BC134479 mRNA Translation: AAI34480.1
PIRiB54717
RefSeqiNP_776579.1, NM_174154.2
UniGeneiBt.5019

Genome annotation databases

GeneIDi281421
KEGGibta:281421

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34261 mRNA Translation: AAA59357.1
BC134479 mRNA Translation: AAI34480.1
PIRiB54717
RefSeqiNP_776579.1, NM_174154.2
UniGeneiBt.5019

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EH5X-ray2.50A28-306[»]
1EI9X-ray2.25A28-306[»]
1EXWX-ray2.40A28-306[»]
ProteinModelPortaliP45478
SMRiP45478
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP45478, 2 interactors
STRINGi9913.ENSBTAP00000017780

Protein family/group databases

ESTHERibovin-ppt Palmitoyl-protein_thioesterase

Proteomic databases

PaxDbiP45478
PRIDEiP45478

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281421
KEGGibta:281421

Organism-specific databases

CTDi5538

Phylogenomic databases

eggNOGiKOG2541 Eukaryota
COG1075 LUCA
HOGENOMiHOG000199232
HOVERGENiHBG018186
InParanoidiP45478
KOiK01074

Miscellaneous databases

EvolutionaryTraceiP45478

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002472 Palm_thioest
IPR030294 PPT1
PANTHERiPTHR11247:SF8 PTHR11247:SF8, 1 hit
PfamiView protein in Pfam
PF02089 Palm_thioest, 1 hit
PRINTSiPR00414 PPTHIESTRASE
SUPFAMiSSF53474 SSF53474, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPT1_BOVIN
AccessioniPrimary (citable) accession number: P45478
Secondary accession number(s): A7YWB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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