UniProtKB - P45464 (LPOA_ECOLI)
Protein
Penicillin-binding protein activator LpoA
Gene
lpoA
Organism
Escherichia coli (strain K12)
Status
Functioni
Regulator of peptidoglycan synthesis that is essential for the function of penicillin-binding protein 1A (PBP1a). Stimulates transpeptidase activity of PBP1a in vitro.2 Publications
GO - Molecular functioni
- enzyme regulator activity Source: UniProtKB
- hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
GO - Biological processi
- peptidoglycan biosynthetic process Source: UniProtKB
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Biological process | Cell shape, Peptidoglycan synthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:G7642-MONOMER MetaCyc:G7642-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Penicillin-binding protein activator LpoAShort name: PBP activator LpoA Alternative name(s): Lipoprotein activator of PBP from the outer membrane A |
Gene namesi | Name:lpoA Synonyms:yraM Ordered Locus Names:b3147, JW3116 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell outer membrane 2 Publications; Lipid-anchor 2 Publications; Periplasmic side 2 Publications Note: Localizes at the sidewall of elongating cells.
GO - Cellular componenti
- cell outer membrane Source: EcoCyc
- periplasmic side of cell outer membrane Source: UniProtKB
- periplasmic space Source: InterPro
Keywords - Cellular componenti
Cell outer membrane, MembranePathology & Biotechi
Disruption phenotypei
Absence of both LpoA and LpoB leads to a decrease in peptide cross-linking and to cell lysis.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Sequence analysisAdd BLAST | 26 | |
ChainiPRO_0000169452 | 27 – 678 | Penicillin-binding protein activator LpoAAdd BLAST | 652 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 27 | N-palmitoyl cysteineSequence analysis | 1 | |
Lipidationi | 27 | S-diacylglycerol cysteineSequence analysis | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
jPOSTi | P45464 |
PaxDbi | P45464 |
PRIDEi | P45464 |
Interactioni
Subunit structurei
Interacts with PBP1a.
2 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4261989, 57 interactors |
DIPi | DIP-12897N |
IntActi | P45464, 4 interactors |
STRINGi | 511145.b3147 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P45464 |
SMRi | P45464 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the LpoA family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3107, Bacteria |
HOGENOMi | CLU_026091_1_1_6 |
InParanoidi | P45464 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
HAMAPi | MF_01890, LpoA, 1 hit |
InterProi | View protein in InterPro IPR007443, LpoA IPR008939, Lytic_TGlycosylase_superhlx_U IPR028082, Peripla_BP_I IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR38038, PTHR38038, 1 hit |
Pfami | View protein in Pfam PF04348, LppC, 1 hit |
SUPFAMi | SSF48435, SSF48435, 1 hit SSF53822, SSF53822, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P45464-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVPSTFSRLK AARCLPVVLA ALIFAGCGTH TPDQSTAYMQ GTAQADSAFY
60 70 80 90 100
LQQMQQSSDD TRINWQLLAI RALVKEGKTG QAVELFNQLP QELNDAQRRE
110 120 130 140 150
KTLLAVEIKL AQKDFAGAQN LLAKITPADL EQNQQARYWQ AKIDASQGRP
160 170 180 190 200
SIDLLRALIA QEPLLGAKEK QQNIDATWQA LSSMTQEQAN TLVINADENI
210 220 230 240 250
LQGWLDLQRV WFDNRNDPDM MKAGIADWQK RYPNNPGAKM LPTQLVNVKA
260 270 280 290 300
FKPASTNKIA LLLPLNGQAA VFGRTIQQGF EAAKNIGTQP VAAQVAAAPA
310 320 330 340 350
ADVAEQPQPQ TVDGVASPAQ ASVSDLTGEQ PAAQPVPVSA PATSTAAVSA
360 370 380 390 400
PANPSAELKI YDTSSQPLSQ ILSQVQQDGA SIVVGPLLKN NVEELLKSNT
410 420 430 440 450
PLNVLALNQP ENIENRVNIC YFALSPEDEA RDAARHIRDQ GKQAPLVLIP
460 470 480 490 500
RSSLGDRVAN AFAQEWQKLG GGTVLQQKFG STSELRAGVN GGSGIALTGS
510 520 530 540 550
PITLRATTDS GMTTNNPTLQ TTPTDDQFTN NGGRVDAVYI VATPGEIAFI
560 570 580 590 600
KPMIAMRNGS QSGATLYASS RSAQGTAGPD FRLEMEGLQY SEIPMLAGGN
610 620 630 640 650
LPLMQQALSA VNNDYSLARM YAMGVDAWSL ANHFSQMRQV QGFEINGNTG
660 670
SLTANPDCVI NRNLSWLQYQ QGQVVPVS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57950.1 U00096 Genomic DNA Translation: AAC76181.1 AP009048 Genomic DNA Translation: BAE77193.1 |
PIRi | G65104 |
RefSeqi | NP_417616.1, NC_000913.3 WP_000249104.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76181; AAC76181; b3147 BAE77193; BAE77193; BAE77193 |
GeneIDi | 57728582 947663 |
KEGGi | ecj:JW3116 eco:b3147 |
PATRICi | fig|511145.12.peg.3242 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57950.1 U00096 Genomic DNA Translation: AAC76181.1 AP009048 Genomic DNA Translation: BAE77193.1 |
PIRi | G65104 |
RefSeqi | NP_417616.1, NC_000913.3 WP_000249104.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MHK | NMR | - | A | 28-256 | [»] | |
6DR3 | X-ray | 2.10 | A | 31-252 | [»] | |
BMRBi | P45464 | |||||
SMRi | P45464 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261989, 57 interactors |
DIPi | DIP-12897N |
IntActi | P45464, 4 interactors |
STRINGi | 511145.b3147 |
Proteomic databases
jPOSTi | P45464 |
PaxDbi | P45464 |
PRIDEi | P45464 |
Genome annotation databases
EnsemblBacteriai | AAC76181; AAC76181; b3147 BAE77193; BAE77193; BAE77193 |
GeneIDi | 57728582 947663 |
KEGGi | ecj:JW3116 eco:b3147 |
PATRICi | fig|511145.12.peg.3242 |
Organism-specific databases
EchoBASEi | EB2631 |
Phylogenomic databases
eggNOGi | COG3107, Bacteria |
HOGENOMi | CLU_026091_1_1_6 |
InParanoidi | P45464 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7642-MONOMER MetaCyc:G7642-MONOMER |
Miscellaneous databases
PROi | PR:P45464 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
HAMAPi | MF_01890, LpoA, 1 hit |
InterProi | View protein in InterPro IPR007443, LpoA IPR008939, Lytic_TGlycosylase_superhlx_U IPR028082, Peripla_BP_I IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR38038, PTHR38038, 1 hit |
Pfami | View protein in Pfam PF04348, LppC, 1 hit |
SUPFAMi | SSF48435, SSF48435, 1 hit SSF53822, SSF53822, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LPOA_ECOLI | |
Accessioni | P45464Primary (citable) accession number: P45464 Secondary accession number(s): Q2M963 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families